The Star Formation History of the Carina Dwarf Galaxy

We have analyzed deep B and V photometry of the Carina dwarf spheroidal reaching below the old main-sequence turnoff to about V = 25. Using simulated color-magnitude diagrams to model a range of star formation scenarios, we have extracted a detailed, global star formation history. Carina experienced three significant episodes of star formation at about 15 Gyr, 7 Gyr, and 3 Gyr. Contrary to the generic picture of galaxy evolution, however, the bulk of star formation, at least 50%, occured during the episode 7 Gyr ago, which may have lasted as long as 2 Gyr. For unknown reasons, Carina formed only 10-20% of its stars at an ancient epoch and then remained quiescent for more than 4 Gyr. The remainder (~30%) formed relatively recently, only 3 Gyr ago. Interest in the local population of dwarf galaxies has increased lately due to their potential importance in the understanding of faint galaxy counts. We surmise that objects like Carina, which exhibits the most extreme episodic behavior of any of the dwarf spheroidal companions to the Galaxy, are capable of contributing to the observed excess of blue galaxies at B = 24 only if the star formation occurred instantaneously.Comment: 23 pages of text, 20 figures, 8 tables. AJ, in pres

The Star Formation History of the Local Group dwarf galaxy Leo I

We present a quantitative analysis of the star formation history (SFH) of the Local Group dSph galaxy Leo I, from the information in its HST [(V-I),I] color-magnitude diagram (CMD). The method we use is based in comparing, via synthetic CMDs, the expected distribution of stars in the CMD for different evolutionary scenarios, with the observed distribution. We consider the SFH to be composed by the SFR(t), the Z(t), the IMF, and a function $\beta(f,q)$, controlling the fraction $f$ and mass ratio distribution $q$ of binary stars. The comparison between the observed CMD and the model CMDs is done through chi-square minimization of the differences in the number of stars in a set of regions of the CMD. Our solution for the SFH of Leo I defines a minimum of chi-square in a well defined position of the parameter space, and the derived SFR(t) is robust, in the sense that its main characteristics are unchanged for different combinations of the remaining parameters. However, only a narrow range of assumptions for Z(t), IMF and $\beta(f,q)$ result in a good agreement between the data and the models, namely: Z=0.0004, a Kroupa et al. (1993) IMF or slightly steeper, and a relatively large fraction of binary stars. Most star formation activity (70% to 80%) occurred between 7 and 1 Gyr ago. At 1 Gyr ago, it abruptly dropped to a negligible value, but seems to have been active until at least ~ 300 Myr ago. Our results don't unambiguously answer the question of whether Leo I began forming stars around 15 Gyr ago, but it appears that the amount of this star formation, if existing at all, would be small.Comment: 25 pages + 14 figures. Accepted by The Astronomical Journa

CCD BV survey of 42 open clusters

We present results of a photometric survey whose aim was to derive structural and astrophysical parameters for 42 open clusters. While our sample is definitively not representative of the total open cluster sample in the Galaxy, it does cover a wide range of cluster parameters and is uniform enough to allow for simple statistical considerations. BV wide-field CCD photometry was obtained for open clusters for which photometric, structural, and dynamical evolution parameters were determined. The limiting and core radii were determined by analyzing radial density profiles. The ages, reddenings, and distances were obtained from the solar metallicity isochrone fitting. The mass function was used to study the dynamical state of the systems, mass segregation effect and to estimate the total mass and number of cluster members. This study reports on the first determination of basic parameters for 11 out of 42 observed open clusters. The angular sizes for the majority of the observed clusters appear to be several times larger than the catalogue data indicate. The core and limiting cluster radii are correlated and the latter parameter is 3.2 times larger on average. The limiting radius increases with the cluster's mass, and both the limiting and core radii decrease in the course of dynamical evolution. For dynamically not advanced clusters, the mass function slope is similar to the universal IMF slope. For more evolved systems, the effect of evaporation of low-mass members is clearly visible. The initial mass segregation is present in all the observed young clusters, whereas the dynamical mass segregation appears in clusters older than about log(age)=8. Low-mass stars are deficient in the cores of clusters older than log(age)=8.5 and not younger than one relaxation time.Comment: 13 pages, 8 figure

Real-time PCR based on SYBR-Green I fluorescence: An alternative to the TaqMan assay for a relative quantification of gene rearrangements, gene amplifications and micro gene deletions

BACKGROUND: Real-time PCR is increasingly being adopted for RNA quantification and genetic analysis. At present the most popular real-time PCR assay is based on the hybridisation of a dual-labelled probe to the PCR product, and the development of a signal by loss of fluorescence quenching as PCR degrades the probe. Though this so-called 'TaqMan' approach has proved easy to optimise in practice, the dual-labelled probes are relatively expensive. RESULTS: We have designed a new assay based on SYBR-Green I binding that is quick, reliable, easily optimised and compares well with the published assay. Here we demonstrate its general applicability by measuring copy number in three different genetic contexts; the quantification of a gene rearrangement (T-cell receptor excision circles (TREC) in peripheral blood mononuclear cells); the detection and quantification of GLI, MYC-C and MYC-N gene amplification in cell lines and cancer biopsies; and detection of deletions in the OPA1 gene in dominant optic atrophy. CONCLUSION: Our assay has important clinical applications, providing accurate diagnostic results in less time, from less biopsy material and at less cost than assays currently employed such as FISH or Southern blotting

Dwarf Cepheids in the Carina Dwarf Spheroidal Galaxy

We have discovered 20 dwarf Cepheids (DC) in the Carina dSph galaxy from the analysis of individual CCD images obtained for a deep photometric study of the system. These short-period pulsating variable stars are by far the most distant (~100 kpc) and faintest (V ~ 23.0) DCs known. The Carina DCs obey a well-defined period-luminosity relation, allowing us to readily distinguish between overtone and fundamental pulsators in nearly every case. Unlike RR Lyr stars, the pulsation mode turns out to be uncorrelated with light-curve shape, nor do the overtone pulsators tend towards shorter periods compared to the fundamental pulsators. Using the period-luminosity (PL) relations from Nemec et al. (1994 AJ, 108, 222) and McNamara (1995, AJ, 109, 1751), we derive (m-M)_0 = 20.06 +/- 0.12, for E(B-V) = 0.025 and [Fe/H] = -2.0, in good agreement with recent, independent estimates of the distance/reddening of Carina. The error reflects the uncertainties in the DC distance scale, and in the metallicity and reddening of Carina. The frequency of DCs among upper main sequence stars in Carina is approximately 3%. The ratio of dwarf Cepheids to RR Lyr stars in Carina is 0.13 +/- 0.10, though this result is highly sensitive to the star-formation history of Carina and the evolution of the Horizontal Branch. We discuss how DCs may be useful to search effectively for substructure in the Galactic halo out to Galactocentric distances of ~100 kpc.Comment: 20 pages of text, 7 figure

Deep HST-WFPC2 photometry of NGC 288. II. The Main Sequence Luminosity Function

The Main Sequence Luminosity Function (LF) of the Galactic globular cluster NGC 288 has been obtained using deep WFPC2 photometry. We have employed a new method to correct for completeness and fully account for bin-to-bin migration due to blending and/or observational scatter. The effect of the presence of binary systems in the final LF is quantified and is found to be negligible. There is a strong indication of the mass segregation of unevolved single stars and clear signs of a depletion of low mass stars in NGC 288 with respect to other clusters. The results are in good agreement with the prediction of theoretical models of the dynamical evolution of NGC 288 that take into account the extreme orbital properties of this cluster.Comment: 16 pages, 6 .ps figures. Low resolution version of fig. 1; full resolution figure soon available at http://www.bo.astro.it/bap/BAPhome.html l. Latex. emulateapj5.sty macro included. Accepted for publication by The Astronomical Journa

The Effect of Star Formation History on the Inferred Initial Stellar Mass Function

Peaks and lulls in the star formation rate (SFR) over the history of the Galaxy produce plateaux and declines in the present day mass function (PDMF) where the main-sequence lifetime overlaps the age and duration of the SFR variation. These PDMF features can be misinterpreted as the form of the intrinsic stellar initial mass function (IMF) if the star formation rate is assumed to be constant or slowly varying with time. This effect applies to all regions that have formed stars for longer than the age of the most massive stars, including OB associations, star complexes, and especially galactic field stars. Related problems may apply to embedded clusters. Evidence is summarized for temporal SFR variations from parsec scales to entire galaxies, all of which should contribute to inferred IMF distortions. We give examples of various star formation histories to demonstrate the types of false IMF structures that might be seen. These include short-duration bursts, stochastic histories with log-normal amplitude distributions, and oscillating histories with various periods and phases. The inferred IMF should appear steeper than the intrinsic IMF over mass ranges where the stellar lifetimes correspond to times of decreasing SFRs; shallow portions of the inferred IMF correspond to times of increasing SFRs. If field regions are populated by dispersed clusters and defined by their low current SFRs, then they should have steeper inferred IMFs than the clusters. The SFRs required to give the steep field IMFs in the LMC and SMC are determined. Structure observed in several determinations of the Milky Way field star IMF can be accounted for by a stochastic and bursty star formation history.Comment: accepted by ApJ for 1 Jan 2006, Vol 636, 12 pages + 6 figure

High dispersion spectroscopy of two A supergiant systems in the Small Magellanic Cloud with novel properties

We present the results of a spectroscopic investigation of two novel variable bright blue stars in the SMC, OGLE004336.91-732637.7 (SMC-SC3) and the periodically occulted star OGLE004633.76-731204.3 (SMC-SC4), whose photometric properties were reported by Mennickent et al. (2010). High-resolution spectra in the optical and far-UV show that both objects are actually A + B type binaries. Three spectra of SMC-SC4 show radial velocity variations, consistent with the photometric period of 184.26 days found in Mennickent et al. 2010. The optical spectra of the metallic lines in both systems show combined absorption and emission components that imply that they are formed in a flattened envelope. A comparison of the radial velocity variations in SMC-SC4 and the separation of the V and R emission components in the Halpha emission profile indicate that this envelope, and probably also the envelope around SMC-SC3, is a circumbinary disk with a characteristic orbital radius some three times the radius of the binary system. The optical spectra of SMC-SC3 and SMC-SC4 show, respectively, HeI emission lines and discrete Blue Absorption Components (BACs) in metallic lines. The high excitations of the HeI lines in the SMC-SC3 spectrum and the complicated variations of FeII emission and absorption components with orbital phase in the spectrum of SMC-SC4 suggests that shocks occur between the winds and various static regions of the stars' co-rotating binary-disk complexes. We suggest that BACs arise from wind shocks from the A star impacting the circumbinary disk and a stream of former wind-efflux from the B star accreting onto the A star. We dub these objects prototype of a small group of Magellanic Cloud wind-interacting A + B binaries.Comment: To be published in MNRA

Structures in surface-brightness profiles of LMC and SMC star clusters: evidence of mergers?

The LMC and SMC are rich in binary star clusters, and some mergers are expected. It is important to characterize single clusters, binary clusters and candidates to mergers. We selected a sample of star clusters in each Cloud with this aim. Surface photometry of 25 SMC and 22 LMC star clusters was carried with the ESO Danish 1.54 m telescope. 23 clusters were observed for the first time for these purposes. We fitted Elson, Fall and Freeman (1987, EFF) profiles to the data, deriving structural parameters, luminosities and masses. We also use isophotal maps to constrain candidates to cluster interactions.} {The structural parameters, luminosities and masses presented good agreement with those in the literature. Three binary clusters in the sample have a double profile. Four clusters (NGC 376, K 50, K 54 and NGC 1810) do not have companions and present as well important deviations from EFF profiles. The present sample contains blue and red Magellanic clusters. Extended EFF profiles were detected in some blue clusters. We find evidence that important deviations from the body of EFF profiles might be used as a tool to detect cluster mergers.Comment: 16 pages and 8 figures. Accepted by A&