22 research outputs found
Diversity of hydrolytic enzymes in haloarchaeal strains isolated from salt lake
Production of ten hydrolytic enzymes was qualitatively studied on the
haloarchaeal strains isolated from Aran-Bidgol hypersaline lake in the
central desert area of Iran. A total of 293 haloarchea strains were
selected among 300 extremely halophilic isolated prokaryotes.
Accordingly, 142, 141, 128, 64, 38, 16, 7, 3 and 1 archaeal isolates
were able to produce DNase, amylase, lipase, inulinase, pullulanase,
protease, cellulase, chitinase and xylanase, respectively. None was
able to produce pectinase activity. Combined hydrolytic activity was
also detected in many strains. A total of 0.3 % of the strains showed 6
hydrolytic activities, 0.3 % of the strains had 5 hydrolytic
activities, 5.4 % of the strains presented 4 hydrolytic activities, 25
% of the strains presented 3 hydrolytic activities, 28 % of the strains
presented 2 hydrolytic activities and 18 % of the strains presented 1
hydrolytic activity. According to their phenotypic characteristics and
comparative partial 16 S rRNA sequence analysis, the halophilic strains
were all identified as members of family Halobacteriaceae within 12
different taxa from the following genera: Halorubrum, Haloarcula,
Natrinema, Halovivax and Natronomonas. Most enzymes production rate was
observed in the genera Halorubrum, Haloarculaand Natrinema whereas;
there was not any detectable amount of enzyme production in the genera
Halovivax and Natronomonas. The most hydrolytic isolate with 6
combinatorial enzyme production belonged to the genus Natrinema. This
investigation showed that the extreme halophilic archaea from
Aran-Bidgol lake are a potential source of hydrolytic enzyme under
stress conditions and may have possess commercial value
Halovenus aranensis gen. nov., sp. nov., an extremely halophilic archaeon from Aran-Bidgol salt lake
Haloarchaeobius iranensis gen. nov., sp. nov., an extremely halophilic archaeon isolated from a saline lake
Spatial and temporal distribution of archaeal diversity in meromictic, hypersaline Ocnei Lake (Transylvanian Basin, Romania)
Halapricum salinum gen. nov., sp. nov., an extremely halophilic archaeon isolated from non-purified solar salt
High metabolomic microdiversity within co-occurring isolates of the extremely halophilic bacterium Salinibacter ruber
Salinibacter ruber is an extremely halophilic member of the Bacteroidetes that thrives in crystallizer ponds worldwide. Here, we have analyzed two sets of 22 and 35 co-occurring S. ruber strains, newly isolated respectively, from 100 microliters water samples from crystalizer ponds in Santa Pola and Mallorca, located in coastal and inland Mediterranean Spain and 350 km apart from each other. A set of old strains isolated from the same setting were included in the analysis. Genomic and taxonomy relatedness of the strains were analyzed by means of PFGE and MALDI-TOF, respectively, while their metabolomic potential was explored with high resolution ion cyclotron resonance Fourier transform mass spectrometry (ICR-FT/MS). Overall our results show a phylogenetically very homogeneous species expressing a very diverse metabolomic pool. The combination of MALDI-TOF and PFGE provides, for the newly isolated strains, the same scenario presented by the previous studies of intra-specific diversity of S. ruber using a more restricted number of strains: the species seems to be very homogeneous at the ribosomal level while the genomic diversity encountered was rather high since no identical genome patterns could be retrieved from each of the samples. The high analytical mass resolution of ICR-FT/MS enabled the description of thousands of putative metabolites from which to date only few can be annotated in databases. Some metabolomic differences, mainly related to lipid metabolism and antibiotic-related compounds, provided enough specificity to delineate different clusters within the co-occurring strains. In addition, metabolomic differences were found between old and new strains isolated from the same ponds that could be related to extended exposure to laboratory conditions.This work was supported by the projects CLG2009-12651-C02-01 and 02; and CE-CSD2007-0005 of the Spanish Ministry of Science and Innovation, and all three projects were also co-financed with FEDER support from the European Union. JBE was financed by the Government of the Balearic Islands, Ministry of Economy and Finances