High-performance liquid chromatography as a technique to measure the competitive adsorption of plasma proteins onto latices

Isotherms of human serum albumin (HSA), human immunoglobulin G (HIgG), and human fibrinogen (HFb) onto a polystyrene (PS)-latex were determined by depletion of protein in the solution, which was either followed by radioactivity measurements or by UV spectroscopy. Different adsorption isotherms for the same protein were obtained when either radioactivity measurements or UV spectroscopy was used as a detection technique. In order to obtain reliable results from competitive protein adsorption experiments, a method based on the use of high-performance liquid chromatography was developed. A strong preferential adsorption of HFb was observed when adsorption studies were carried out with mixtures of HSA, HFb, and HIgG. When adsorption studies were carried out with solutions containing HSA monomer and dimer, a strong preferential adsorption of HSA dimer was also observed

Endometrial injury in women undergoing assisted reproductive techniques

ACKNOWLEDGEMENTS We would like to express our appreciation to Dra Abha Maheshwari for her important authorial contribution to the previous version of this review. We also acknowledge the important help provided by the Cochrane Menstrual Disorders and Subfertility Group team, specially by Marian Showell, Trials Search Co-ordinator; by Helen Nagels, Managing Editor; and by Prof. Cindy Farquhar, Co-ordinating Editor. Finally, we would like to express our gratitude to the following investigators, who provided essential information for the preparation of this review: TK Aleyamma, Erin F Wolff, Lukasz Polanski, Nava Dekel, Neeta Singh, Suleyman Guven and Tracy YeungPeer reviewedPublisher PD

Blending PEG-based polymers and their use in surface micro-patterning by the FIMIC method to obtain topographically smooth patterns of elasticity

Dieser Beitrag ist mit Zustimmung des Rechteinhabers aufgrund einer (DFG geförderten) Allianz- bzw. Nationallizenz frei zugänglich.This publication is with permission of the rights owner freely accessible due to an Alliance licence and a national licence (funded by the DFG, German Research Foundation) respectively.We have designed and fabricated a library of polyethylene glycol (PEG)-based polymer blends, including blends of two PEG-based polymers that are liquid at room temperature where the optimisation of the blending method allows for the incorporation of higher molecular-weight PEG-based polymers which are solid at room temperature. The absence of a solvent in these blends makes them perfect candidates for use in our recently developed Fill-Molding in Capillaries (FIMIC) patterning method. As our FIMIC samples have shown to be not completely smooth (a small topography up to several nanometers has been seen previously), and this is likely to affect the cellular behaviour, we have improved our technique in order to obtain virtually smooth samples that exhibit a pattern of elasticity only. It is demonstrated that, by taking advantage of the differential swelling of the pattern components, we can level out the undesired topographic difference. In particular, by employing blends of materials, (1) the swelling degree of each component can be fine-tuned to even out any topography and (2) the use of the same blends in the sample, yet with varying cross-linker amounts, ensures the swelling degree and elasticity change without changing the surface chemistry significantly. Genuine, binary patterns of elasticity can thus be fabricated, which are a great asset to study cell migration phenomena in systematic detail.DFG, EXC 314, Unifying Concepts in Catalysi

VALUE study : a protocol for a qualitative semi-structured interview study of IVF add-ons use by patients, clinicians and embryologists in the UK and Australia

Funding This research was funded by an Obstetrics and Gynaecology Innovation Grant from the University of Melbourne Australia. MP is supported by a University of Melbourne Department of Obstetrics and Gynaecology MCR Fellowship. Acknowledgements Our heartfelt thanks to our PPI panel who gave up their time to contribute to the development of VALUE. UK: Isabella Dash, Jennifer Nisbett, Hannah Reid, Ally Richardson, Victoria Thomas, Bassel Wattar. Australia: Katherine Gobbi, Hilary Smith, Natasha Devetak, Alex Polyakov, Anna Ninnis, Lisa Lee, Vadim Mirmilstein.Peer reviewedPublisher PD

Why are feasibility studies accessing routinely collected health data? A systematic review

BACKGROUND: Feasibility trials are often undertaken to determine whether a larger randomised controlled trial (RCT) is achievable. In a recent review, 15 feasibility trials accessed routinely collected health data (RCHD) from UK national databases and registries. This paper looks at attributes of these trials and the reasons why they accessed RCHD. METHODS: We extracted data from all publicly available sources for the 15 feasibility studies found in a previous review of trials successfully accessing RCHD in the UK between 2013–2018 for the purpose of informing or supplementing participant data. We extracted trial characteristics, the registry accessed, and the way the RCHD was used. RESULTS: The 15 feasibility RCTs were conducted in a variety of disease areas, and were generally small (median sample size 100, range 41–4061) and individually randomised (60%, 9/15). The primary trial outcome was predominantly administrative (non-clinical) (80%, 12/15) such as feasibility of patient recruitment. They were more likely to recruit from secondary care (67%, 10/15) settings than primary (33%, 5/15). NHS Digital was the most commonly accessed registry (33% (5/15)) with SAIL databank (20% (3/15)), electronic Data Research and Innovation Service (eDRIS) and Paediatric Intensive Care Audit Network (PICANET) (each 13% 2/15) also being accessed. Where the information was clear, the trials used RCHD for data collection during the trial (47%, 7/15), follow-up after the trial (27%, 4/15) and recruitment (13%, 2/15). CONCLUSIONS: Between 2013 and 2018, 15 feasibility trials successfully accessed UK RCHD. Feasibility trials would benefit, as with other trials, from guidance on reporting the use of RCHD in protocols and publications

Особенности функционирования системы здравоохранения Томской области

Рассмотрены основные проблемы функционирования отрасли здравоохранения в Томской области в настоящее время. Предложены направления, в которых прописаны этапы, мероприятия, объем финансирования и ожидаемые результаты реализации предложенных направлений, за счет которых предполагается привести отрасль в соответствие современным трендам и повысить качество жизни населения

Nermin

Yenişehirlizade Halit Eyüp'ün Servet'te tefrika edilen Nermin adlı roman

Can Genetic Programming Do Manifold Learning Too?

Exploratory data analysis is a fundamental aspect of knowledge discovery that aims to find the main characteristics of a dataset. Dimensionality reduction, such as manifold learning, is often used to reduce the number of features in a dataset to a manageable level for human interpretation. Despite this, most manifold learning techniques do not explain anything about the original features nor the true characteristics of a dataset. In this paper, we propose a genetic programming approach to manifold learning called GP-MaL which evolves functional mappings from a high-dimensional space to a lower dimensional space through the use of interpretable trees. We show that GP-MaL is competitive with existing manifold learning algorithms, while producing models that can be interpreted and re-used on unseen data. A number of promising future directions of research are found in the process.Comment: 16 pages, accepted in EuroGP '1