Extracellular vesicle signatures and protein citrullination are modified in shore crabs (Carcinus maenas) infected with Hematodinium sp

Epizootiologists recurrently encounter symbionts and pathobionts in the haemolymph (blood equivalent) of shellfish. One such group is the dinoflagellate genus , which contains several species that cause debilitating disease in decapod crustaceans. The shore crab acts as a mobile reservoir of microparasites, including sp., thereby posing a risk to other co-located commercially important species, e.g. velvet crabs ( ). Despite the widespread prevalence and documented seasonality of infection dynamics, there is a knowledge gap regarding host-pathogen antibiosis, namely, how avoids the host's immune defences. Herein, we interrogated the haemolymph of -positive and -negative crabs for extracellular vesicle (EV) profiles (a proxy for cellular communication), alongside proteomic signatures for post-translational citrullination/deimination performed by arginine deiminases, which can infer a pathologic state. Circulating EV numbers in parasitized crab haemolymph were reduced significantly, accompanied by smaller EV modal size profiles (albeit non-significantly) when compared to -negative controls. Differences were observed for citrullinated/deiminated target proteins in the haemolymph between the parasitized and control crabs, with fewer hits identified overall in the former. Three deiminated proteins specific to parasitized crab haemolymph were actin, Down syndrome cell adhesion molecule (DSCAM), and nitric oxide synthase - factors that contribute to innate immunity. We report, for the first time, sp. could interfere with EV biogenesis, and that protein deimination is a putative mechanism of immune-modulation in crustacean- interactions

Long Term Potentiation (LTP) and Long Term Depression (LTD) Cause Differential Spatial Redistribution of the Synaptic Vesicle Protein Synaptophysin in the Middle Molecular Layer of the Dentate Gyrus in Rat Hippocampus

The presynaptic modifications that accompany long-term changes in synaptic plasticity are still not fully understood. Synaptophysin is a major synaptic vesicle protein involved in neurotransmitter release. We have used quantitative electron microscopy to study synaptophysin (Syn) immunolabelling in the hippocampus of adult rats 24h after induction in vivo of long term potentiation (LTP), and long term depression (LTD). Electrodes were implanted chronically in hippocampus with stimulation at either the medial (MPP) or lateral perforant path (LPP). 24h following induction of LTP or LTD rats were rapidly perfusion fixed and hippocampal tissue processed to electron microscopy via freeze substitution method. Anti-synaptophysin post-embedding immunolabelling was performed and tissue was imaged in the middle molecular layer (MML) of the dentate gyrus. There was a significant decrease in number of Syn labelled vesicles per unit area of bouton after LTP, but not LTD. An analysis of the spatial distribution of Syn labelled synaptic vesicles showed an increase in nearest neighbour distances, more so in the LTP than the LTD group, which is consistent with the overall decrease of Syn after LTP. These data are in agreement with the suggestion that Syn is involved in clathrin-dependent and “kiss and run” endocytosis which occurs perisynaptically. Thus, an increase in release of neurotransmitter and in consequence endocytosis would be consistent with an increased active zone distance for vesicles containing Syn

The X-ray spectra of the flaring and quiescent states of YZ CMi observed by XMM-Newton

We analyse the X-ray spectrum of the active late-type star YZ CMi (M4.5V); for quiescent as well as active stages, we derive emission measure (EM) distributions, elemental abundances, and electron temperatures and densities, which are in turn used to estimate flare loop lengths as well as coronal magnetic field strengths. YZ CMi was observed in the wavelength range 1-40 Å by the X-ray detectors RGS, EPIC-MOS and EPIC-pn onboard XMM-Newton. Some flares occurred during the observation. We perform a multi-temperature fit and model the differential EM of both the flaring and the quiescent parts of the spectrum and derive the coronal temperature distribution, EMs, and elemental abundances of the flaring and quiescent states. The observed temperature covers a range from about 1.3 to 42 MK. The total volume EM in this temperature interval is 13.7 ± .8 × 1050 cm−3 for the quiescent state and 21.7 ± 1.4 × 1050 cm−3 for the active state. The abundance pattern in the quiescent state shows some depletion of low first ionization potential (FIP) elements relative to high-FIP elements, indicating the presence of an I(nverse)FIP effect in this active star. No abundance differences between the quiescent and the active states are established. Based on the X-ray light curves in combination with the temperature, density and EM, the coronal magnetic field strength at flare-site is found to be between 50 and 100 G and the flaring loop lengths are estimated to be in the range of 5 -13 × 109 c

Properties of high-degree oscillation modes of the Sun observed with Hinode/SOT

Aims. With the Solar Optical Telescope on Hinode, we investigate the basic properties of high-degree solar oscillations observed at two levels in the solar atmosphere, in the G-band (formed in the photosphere) and in the Ca II H line (chromospheric emission). Methods. We analyzed the data by calculating the individual power spectra as well as the cross-spectral properties, i.e., coherence and phase shift. The observational properties are compared with a simple theoretical model, which includes the effects of correlated noise. Results. The results reveal significant frequency shifts between the Ca II H and G-band spectra, in particular above the acoustic cut-off frequency for pseudo-modes. The cross-spectrum phase shows peaks associated with the acoustic oscillation (p-mode) lines, and begins to increase with frequency around the acoustic cut-off. However, we find no phase shift for the (surface gravity wave) f-mode. The observed properties for the p-modes are qualitatively reproduced in a simple model with a correlated background if the correlated noise level in the Ca II H data is higher than in the G-band data. These results suggest that multi-wavelength observations of solar oscillations, in combination with the traditional intensity-velocity observations, may help to determine the level of the correlated background noise and to determine the type of wave excitation sources on the Sun.Comment: 4 pages, 3 figures (6 plots), accepted by A&A Letters for Hinode special issue. v2 includes minor changes suggested by the referee (incl. math. definitions) as well as edited languag

Performance of Geant4 in simulating semiconductor particle detector response in the energy range below 1 MeV

Geant4 simulations play a crucial role in the analysis and interpretation of experiments providing low energy precision tests of the Standard Model. This paper focuses on the accuracy of the description of the electron processes in the energy range between 100 and 1000 keV. The effect of the different simulation parameters and multiple scattering models on the backscattering coefficients is investigated. Simulations of the response of HPGe and passivated implanted planar Si detectors to \beta{} particles are compared to experimental results. An overall good agreement is found between Geant4 simulations and experimental data

Protein Deimination Signatures in Plasma and Plasma-EVs and Protein Deimination in the Brain Vasculature in a Rat Model of Pre-Motor Parkinson’s Disease

The identification of biomarkers for early diagnosis of Parkinson’s disease (PD) is of pivotal importance for improving approaches for clinical intervention. The use of translatable animal models of pre-motor PD therefore offers optimal opportunities for novel biomarker discovery in vivo. Peptidylarginine deiminases (PADs) are a family of calcium-activated enzymes that contribute to protein misfolding through post-translational deimination of arginine to citrulline. Furthermore, PADs are an active regulator of extracellular vesicle (EV) release. Both protein deimination and extracellular vesicles (EVs) are gaining increased attention in relation to neurodegenerative diseases, including in PD, while roles in pre-motor PD have yet to be investigated. The current study aimed at identifying protein candidates of deimination in plasma and plasma-EVs in a rat model of pre-motor PD, to assess putative contributions of such post-translational changes in the early stages of disease. EV-cargo was further assessed for deiminated proteins as well as three key micro-RNAs known to contribute to inflammation and hypoxia (miR21, miR155, and miR210) and also associated with PD. Overall, there was a significant increase in circulating plasma EVs in the PD model compared with sham animals and inflammatory and hypoxia related microRNAs were significantly increased in plasma-EVs of the pre-motor PD model. A significantly higher number of protein candidates were deiminated in the pre-motor PD model plasma and plasma-EVs, compared with those in the sham animals. KEGG (Kyoto encyclopedia of genes and genomes) pathways identified for deiminated proteins in the pre-motor PD model were linked to “Alzheimer’s disease”, “PD”, “Huntington’s disease”, “prion diseases”, as well as for “oxidative phosphorylation”, “thermogenesis”, “metabolic pathways”, “Staphylococcus aureus infection”, gap junction, “platelet activation”, “apelin signalling”, “retrograde endocannabinoid signalling”, “systemic lupus erythematosus”, and “non-alcoholic fatty liver disease”. Furthermore, PD brains showed significantly increased staining for total deiminated proteins in the brain vasculature in cortex and hippocampus, as well as increased immunodetection of deiminated histone H3 in dentate gyrus and cortex. Our findings identify EVs and post-translational protein deimination as novel biomarkers in early pre-motor stages of PD

Photocatalytic activity of titania nanopowders prepared by a sol–gel process at various pHs

A strategy has been proposed to prepare photocatalytically active titania nanopowders through a sol-gel route using high-degree molecular separation upon the dilution of reagents, high water/alkoxide ratios, high reagent mixing rates, and pH effects. This strategy has been successfully used to isolate, from sols, anatase powders with high surface areas (100–310 m2/g) dependent on the pH value during the synthesis. The photocatalytic activity of titania nanopowders prepared by the sol-gel process at various pHs has been tested in photodestruction of organic dyes (Rodamine B, Methylene Blue, and Anthraquinone AcidBlue) in acid solutions. UV-radiation-induced dye destruction rates are found to depend on the surface properties (including surface area and ζ potential) and hydration specifics of the titania

Suspension of Mitotic Activity in Dentate Gyrus of the Hibernating Ground Squirrel

Neurogenesis occurs in the adult mammalian hippocampus, a region of the brain important for learning and memory. Hibernation in Siberian ground squirrels provides a natural model to study mitosis as the rapid fall in body temperature in 24 h (from 35-36°C to +4–6°C) permits accumulation of mitotic cells at different stages of the cell cycle. Histological methods used to study adult neurogenesis are limited largely to fixed tissue, and the mitotic state elucidated depends on the specific phase of mitosis at the time of day. However, using an immunohistochemical study of doublecortin (DCX) and BrdU-labelled neurons, we demonstrate that the dentate gyrus of the ground squirrel hippocampus contains a population of immature cells which appear to possess mitotic activity. Our data suggest that doublecortin-labelled immature cells exist in a mitotic state and may represent a renewable pool for generation of new neurons within the dentate gyrus

A nanoflare model of quiet Sun EUV emission

Nanoflares have been proposed as the main source of heating of the solar corona. However, detecting them directly has so far proved elusive, and extrapolating to them from the properties of larger brightenings gives unreliable estimates of the power-law exponent $\alpha$ characterising their distribution. Here we take the approach of statistically modelling light curves representative of the quiet Sun as seen in EUV radiation. The basic assumption is that all quiet-Sun EUV emission is due to micro- and nanoflares, whose radiative energies display a power-law distribution. Radiance values in the quiet Sun follow a lognormal distribution. This is irrespective of whether the distribution is made over a spatial scan or over a time series. We show that these distributions can be reproduced by our simple model.Comment: 13 pages, 18 figures, accepted for publication by A&

Multi-wavelength spatially resolved analysis of quasi-periodic pulsations in a solar flare

Aims. We aim to perform a spatially resolved analysis of a quasi-periodic pulsation event from 8th May 1998 using microwave data from the Nobeyama Radioheliograph and Radiopolarimeter, and X-ray data from the Yohkoh satellite. Methods. Time spectra of the signals integrated over the emission source are constructed with the use of the Lomb-Scargle periodogram method, revealing the presence of a pronounced 16 s periodicity. The Pixon image reconstruction algorithm and Hanaoka algorithm are used to reconstruct images from the hard X-ray data from Yohkoh/HXT and Nobeyama Radioheliograph respectively. The phase relationship of the microwave emission was analysed with the use of cross-correlation techniques. Results. The flaring loop was resolved in the microwave band. The hard X-ray sources are found to be located near the footpoint and at the loop apex determined by the soft X-ray image. The apex source is much fainter than footpoint one. In microwave, all parts of the loop are seen to oscillate with the same period and almost in phase. It was not possible to determine the spatial structure of the oscillation in the hard X-ray band. The period and the coherent spatial structure of the oscillation are indicative of the presence of either an MHD sausage mode or a periodic regime of magnetic reconnectio