RR Lyrae - Theory vs Observation

The luminosities, effective temperatures and metallicities that are derived empirically by Kovacs and Jurcsik from the light curves of a large number of globular cluster and field RRab and RRc stars are compared to theoretical RR Lyrae models. The strong luminosity dependence of the empirical blue and red edges (Log L vs Log Teff diagram) is in disagreement with that of both radiative and convective models. A reexamination of the theoretical uncertainties in the modelling leads us to conclude that the disagreement is irreconcilable.Comment: 6 pages, 5 figures (revised april 2000, revisions relatively minor

Hydrodynamical Survey of First Overtone Cepheids

A hydrodynamical survey of the pulsational properties of first overtone Galactic Cepheids is presented. The goal of this study is to reproduce their observed light- and radial velocity curves. The comparison between the models and the observations is made in a quantitative manner on the level of the Fourier coefficients. Purely radiative models fail to reproduce the observed features, but convective models give good agreement. It is found that the sharp features in the Fourier coefficients are indeed caused by the P1/P4 = 2 resonance, despite the very large damping of the 4th overtone. For the adopted mass-luminosity relation the resonance center lies near a period of 4.2d +/- 0.2 as indicated by the observed radial velocity data, rather than near 3.2d as the light-curves suggest.Comment: ApJ, 12 pages, (slightly) revise

Turbulent convection: comparing the moment equations to numerical simulations

The non-local hydrodynamic moment equations for compressible convection are compared to numerical simulations. Convective and radiative flux typically deviate less than 20% from the 3D simulations, while mean thermodynamic quantities are accurate to at least 2% for the cases we have investigated. The moment equations are solved in minutes rather than days on standard workstations. We conclude that this convection model has the potential to considerably improve the modelling of convection zones in stellar envelopes and cores, in particular of A and F stars.Comment: 10 pages (6 pages of text including figure captions + 4 figures), Latex 2e with AAS Latex 5.0 macros, accepted for publication in ApJ

RR Lyrae Variables in the Globular Cluster M55. The First Evidence for Non Radial Pulsations in RR Lyr Stars

We present the results of a photometric study of RR Lyrae variables in the field of the globular cluster M55. We have discovered nine new RR Lyrae stars, increasing the number of known variables in this cluster to 15 objects. Five of the newly discovered variables belong to Bailey type RRc and two to type RRab. Two background RRab stars are probable members of the Sagittarius dwarf galaxy. Fourier decomposition of the light curves was used to derive basic properties of the present sample of RR Lyrae variables. From an analysis of the RRc variables we obtain a mean mass of $M=0.53\pm0.03 M_\odot$, luminosity $\log L=1.75\pm0.01$, effective temperature $T_{eff}=7193\pm27$ K, and helium abundance $Y=0.27\pm0.01$. Based on the $B-V$ colors, periods and metallicities of the RRab stars we estimate the value of the color excess for M55 to be equal to $E(B-V)=0.11\pm0.03$. Using this value we derive the colors of the blue and red edges of the instability strip in M55. The blue edge lies at $(B-V)_0=0.20$ mag and the red edge lies at $(B-V)_0=0.38$ mag. We estimate the values of the visual apparent and dereddened distance moduli to be $13.65\pm0.11$ and $13.31\pm0.11$, respectively. The light curves of three of the RRc variables exhibit changes in amplitude of over 0.1 mag on the time scale of less than a week, rather short for the Blazhko effect, but with no evidence for another radial pulsational frequency. However we do detect other periodicities which are clearly visible in the light curve after removing variations with the first overtone radial frequency. This is strong evidence for the presence of non-radial pulsations, a behavior common for $\delta$ Scuti stars but not yet observed among RR Lyr variables.Comment: submitted to Astronomical Journal, 33 pages with 11 figure

Double-Mode Stellar Pulsations

The status of the hydrodynamical modelling of nonlinear multi-mode stellar pulsations is discussed. The hydrodynamical modelling of steady double-mode (DM) pulsations has been a long-standing quest that is finally being concluded. Recent progress has been made thanks to the introduction of turbulent convection in the numerical hydrodynamical codes which provide detailed results for individual models. An overview of the modal selection problem in the HR diagram can be obtained in the form of bifurcation diagrams with the help of simple nonresonant amplitude equations that capture the DM phenomenon.Comment: 34 pages, to appear as a chapter in Nonlinear Stellar Pulsation in the Astrophysics and Space Science Library (ASSL), Editors: M. Takeuti & D. Sasselov (prints double column with pstops '2:[email protected](22.0cm,-2cm)[email protected](22.0cm,11.0cm)' in.ps out.ps

Association between adenovirus viral load and mortality in pediatric allo-hct recipients. the multinational advance study

This multivariable analysis from the AdVance multicenter observational study assessed adenovirus (AdV) viremia peak, duration, and overall AdV viral burden—measured as time-averaged area under the viremia curve over 16 weeks (AAUC0-16)—as predictors of all-cause mortality in pediatric allo-HCT recipients with AdV viremia. In the 6 months following allo-HCT, 241 patients had AdV viremia ≥ 1000 copies/ml. Among these, 18% (43/241) died within 6 months of first AdV ≥ 1000 copies/ml. Measures of AdV viral peak, duration, and overall burden of infection consistently correlate with all-cause mortality. In multivariable analyses, controlling for lymphocyte recovery, patients with AdV AAUC0-16 in the highest quartile had a hazard ratio of 11.1 versus the lowest quartile (confidence interval 5.3–23.6); for peak AdV viremia, the hazard ratio was 2.2 for the highest versus lowest quartile. Both the peak level and duration of AdV viremia were correlated with short-term mortality, independent of other known risk factors for AdV-related mortality, such as lymphocyte recovery. AdV AAUC0-16, which assesses both peak and duration of AdV viremia, is highly correlated with mortality under the current standard of care. New therapeutic agents that decrease AdV AAUC0-16 have the potential of reducing mortality in this at-risk patient population

V440 Per: the longest period overtone Cepheid

V440 Per is a Population I Cepheid with the period of 7.57 day and low amplitude, almost sinusoidal light and radial velocity curves. With no reliable data on the 1st harmonic, its pulsation mode identification remained controversial. We obtained a radial velocity curve of V440 Per with our new high precision and high throughput Poznan Spectroscopic Telescope. Our data reach the accuracy of 130 m/s per individual measurement and yield a secure detection of the 1st harmonic with the amplitude of A_2= 140+/- 15 m/s. The velocity Fourier phase \phi_21 of V440 Per is inconsistent at the 7.25 \sigma level with those of the fundamental mode Cepheids, implying that the star must be an overtone Cepheid, as originally proposed by Kienzle et al.(1999). Thus, V440 Per becomes the longest period Cepheid with the securely established overtone pulsations. We show, that the convective nonlinear pulsation hydrocode can reproduce the Fourier parameters of V440 Per very well. Requirement to match the observed properties of V440 Per constrains free parameters of the dynamical convection model used in the pulsation calculations, in particular the radiative losses parameter.Comment: Submitted to MNRA

A two-columns formalism for time-dependent modelling of stellar convection. I. Description of the method

Despite all advances in multi-dimensional hydrodynamics, investigations of stellar evolution and stellar pulsations still depend on one-dimensional computations. The present work devises an alternative to the mixing length theory or turbulence models usually adopted for the modelling of convective transport in such studies. Assuming that the largest convective patterns generate the majority of convective transport, the convective velocity field is described using two parallel radial columns to represent up- and downstream flows. Horizontal exchange in the form of fluid flow and radiation over their connecting interface couples the two columns and allows a simple circulating motion. The main parameters of this convective description have a straightforward geometrical meaning, namely the diameter of the columns (corresponding to the size of the convective cells) and the ratio of cross section between up- and downdrafts. For this geometrical setup, the time-dependent solution of the equations of radiation hydrodynamics is computed from an implicit scheme which has the advantage of being not affected by the Courant-Friedrichs-Lewy time step limit. In order to demonstrate the approach, results for the example of convection zones in Cepheids are presented

Near-IR spectra of ISOGAL sources in the Inner Galactic Bulge

In this work we present near-IR spectra (HK-band) of a sample of 107 sources with mid-IR excesses at 7 and 15 $\rm \mu$m detected during the ISOGAL survey. Making use of the DENIS interstellar extinction map from Schultheis et al. (1999) we derive luminosities and find that the $\rm M_{bol}$ vs.~$\rm ^{12}CO$ and $\rm M_{bol} vs. H_{2}O$ diagrams are powerful tools for identifying supergiants, AGB stars, giants and young stellar objects. The majority of our sample are AGB stars (~ 80%) while we find four good supergiant candidates, nine young stellar objects and 12 RGB candidates. We have used the most recent $\rm K_{0}-[15]$ relation by Jeong et al. (2002) based on recent theoretical modeling of dust formation of AGB stars to determine mass-loss rates. However, the uncertainties in the mass-loss rates are rather large. The mass-loss rates of the supergiants are comparable with those in the solar neighbourhood while the long-period Variables cover a mass-loss range from $\rm -5 < log \dot{\it{M}} < -7$. The red giant candidateslie at the lower end of the mass-loss rate range between $\rm -6.5 < log \dot{{\it{M}}} < -9$. We used the equivalent width of the CO bandhead at 2.3 $\rm \mu m$, the NaI doublet and the CaI triplet to estimate metallicities using the relation by Ram\'{\i}rez et al. (\cite{Ramirez2000}). The metallicity distribution of the ISOGAL objects shows a mean [Fe/H] $\sim$ -0.25 dex with a dispersion of $\rm \pm 0.40 dex$ which is in agreement with the values of Ram\'{i}rez et al. (\cite{Ramirez2000}) for Galactic Bulge fields between $\rm b = -4^{o}$ and $\rm b = -1.3^{o}$. A comparison with the solar neighbourhood sample of Lan\c{c}on & Wood (LW) shows that our sample is ~ 0.5 dex more metal-rich on average.Comment: 23 pages, 9 figures, 2 appendix with IR spectra. accepted for A&

Genome‐wide off‐target analyses of CRISPR/Cas9‐mediated T‐cell receptor engineering in primary human T cells

Objectives Exploiting the forces of human T cells for treatment has led to the current paradigm of emerging immunotherapy strategies. Genetic engineering of the T-cell receptor (TCR) redirects specificity, ablates alloreactivity and brings significant progress and off-the-shelf options to emerging adoptive T-cell transfer (ACT) approaches. Targeted CRISPR/Cas9-mediated double-strand breaks in the DNA enable knockout or knock-in engineering. Methods Here, we perform CRISPR/Cas9-mediated TCR knockout using a therapeutically relevant ribonucleoprotein (RNP) delivery method to assess the safety of genetically engineered T-cell products. Whole-genome sequencing was performed to analyse whether CRISPR/Cas9-mediated DNA double-strand break at the TCR locus is associated with off-target events in human primary T cells. Results TCRα chain and TCRβ chain knockout leads to high on-target InDel frequency and functional knockout. None of the predicted off-target sites could be confirmed experimentally, whereas whole-genome sequencing and manual Integrative Genomics Viewer (IGV) review revealed 9 potential low-frequency off-target events genome-wide. Subsequent amplification and targeted deep sequencing in 7 of 7 evaluable loci did not confirm these low-frequency InDels. Therefore, off-target events are unlikely to be caused by the CRISPR/Cas9 engineering. Conclusion The combinatorial approach of whole-genome sequencing and targeted deep sequencing confirmed highly specific genetic engineering using CRISPR/Cas9-mediated TCR knockout without potentially harmful exonic off-target effects