Effect of short-term probiotic Enterococcus faecium SF68 dietary supplementation in overweight and obese cats without comorbidities

Obesity in cats is associated with metabolic abnormalities and increased susceptibility to diseases such as diabetes mellitus. Studies in mouse models and human beings have shown that probiotics can reduce food intake, promote weight loss and improve metabolic profile. Studies assessing the effects of probiotics on these same parameters are absent in cats. Therefore, the aim of this study was to determine if probiotic Enterococcus faecium strain SF68 dietary supplementation reduces food intake, promotes weight loss and improves metabolic profile in overweight and obese cats without comorbidities. Twenty overweight and obese specific pathogen-free cats without comorbidities were acclimatised to a dry diet for four weeks. After exclusion of four cats for unrelated reasons, eight cats received a daily oral probiotic for eight weeks and eight control cats received no probiotic. All cats were fed ad libitum with food intake measured daily and bodyweight weekly. Blood was collected at three time points: after four weeks of acclimatisation to the diet, after eight weeks of intervention and after six weeks of washout for measurement of glucose, triglyceride, cholesterol, fructosamine, insulin, leptin, total adiponectin and deuterium oxide for body composition. There were no differences in food intake, metabolic parameters and body composition between the probiotic and control groups after eight weeks of intervention and six weeks of washout (P≥0.050). Short-term use of E faecium SF68 dietary supplementation had no significant effect on food intake, bodyweight, body composition or metabolic parameters in overweight and obese specific pathogen-free cats without comorbidities

TRENDS IN POPULATION SIZE OF OPHRYS ARGOLICA SUBSP. BISCUTELLA IN THE APPENNINO LUCANO-VAL D’AGRI-LAGONEGRESE NATIONAL PARK (ITALY)

n this study we focused on population monitoring of Ophrys argolica subsp. biscutella, an orchid endemic to the south of the Italian Peninsula belonging to the section Araniferae. The study concerned its main detected population located within the Appennino Lucano-Val d'Agri-Lagonegrese National Park (Basilicata Region, South Italy). Ten permanent plots of 10 × 10 m were annually surveyed from 2010 to 2015 using total counts of flowering plants. The following characters were measured: height of inflorescences, number of flowers, lost plants, fruiting plants and fruit set. Percentage cover of trees, shrubs, herbs and bare rocks were visually estimated in 1 × 1 m subplots and an accurate vegetation map of each plot was drawn. In regards to plant height and reproductive variables, no significant differences between the plots were detected. Similarly, with respect to population variables, no significant correlations with vegetation and environmental features (i.e. slope, aspect) emerged. On the contrary, a strong negative trend in the population size was observed (from 237 individuals in 2010 to 61 in 2015) and the number of plants per plot decreased from 23.7 individuals to 6.1 individuals. Reproductive variables (i.e. number of flowers, fruit set) showed a similar decreasing pattern. As for the lost of individuals we detected a higher incidence in 2011 (average number of lost plants per plot was 16.6) with 75% of lost spikes and fruit set amounting to zero. Although grazing management can heavily influence demographic parameters of Ophrys populations, there was no evidence of any kind of land cover or land use change. No appreciable differences were observed in vegetation cover, grazing type or grazing load. Moreover, no significant disturbance events occurred in this area. Finally, the inspection of meteorological data recorded during the monitoring interval lead to argue that the strong demographic decline of the Ophrys argolica subsp. biscutella population in the Appennino Lucano-Val d'Agri-Lagonegrese National Park must be mainly related to the late frost event that occurred in the spring of 2011

Impact of blood tube additives and timing of sampling on blood taurine concentrations in clinically healthy dogs

Introduction: Dilated cardiomyopathy can be associated with taurine deficiency in dogs. Blood taurine concentrations can be analyzed in whole blood (WB) and plasma. The study objectives were to investigate agreement between taurine concentrations measured in WB, heparin plasma, and EDTA plasma, deter-mine intraindividual variation in healthy dogs, and evaluate if time from feeding to sampling impacts concentrations. Animals: 10 English Cocker spaniels and 10 dogs of various breeds. Materials and methods: Dogs were fasted 12 h prior to initial blood sampling, and the blood was collected at five occasions over 8 h. Food was offered immediately after first and 1 h after fourth sampling time point. Results: Agreement between taurine concentrations in EDTA plasma and hepari-nized plasma was good (mean difference 4.5 nmol/mL, 95% confidence interval (CI) 36.8-45.8 nmol/mL). WB concentrations were systematically higher than EDTA and heparin plasma concentrations (mean difference 132.7 nmol/mL, 95% CI 23.6-241.8 nmol/mL, and 127.6 nmol/mL, 95% CI 28.6-226.6 nmol/mL, respectively, all P < 0.001). Intraindividual daily variations in taurine concentration were seen in all additives, with largest variations in plasma (P < 0.001). Taurine concentration in heparinized plasma was higher at first and fifth sampling time points compared to the fourth (P 1/4 0.014). Discussion: Agreement was found between taurine concentrations measured in dif-ferent additives, with expected higher concentration in WB than plasma. Taurine concentrations measured in heparinized plasma varied with sampling time point. Intraindividual daily variations were observed in all additives, but mainly in plasma samples. Conclusion: Taurine concentrations in dogs with suspected deficiency should be in-terpreted with caution. 2022 The Authors. Published by Elsevier B.V

A review of phosphorus homeostasis and the impact of different types and amounts of dietary phosphate on metabolism and renal health in cats

Elevated concentrations of serum phosphate are linked with progression and increased case fatality rate in animals and humans with chronic kidney disease. Elevated concentrations of serum phosphate can be a risk factor for development of renal and cardiovascular diseases or osteoporosis in previously healthy people. In rodents, an excess intake of dietary phosphorus combined with an inverse dietary calcium : phosphorus ratio (<1 : 1) contributes to renal calcification. Renal injury also has occured in cats fed experimental diets supplemented with highly soluble phosphate salts, especially in diets with inverse calcium : phosphorus ratios. However, not all phosphorus sources contribute similarly to this effect. This review, which focuses on cats, summarizes the published evidence regarding phosphorus metabolism and homeostasis, including the relative impact of different dietary phosphorus sources, and their impact on the kidneys. No data currently shows that commercial cat foods induce renal injury. However, some diets contain high amounts of phosphorus relative to recommendations and some have inverse Ca : P ratios and so could increase the risk for development of kidney disease. While limiting the use of highly soluble phosphates appears to be important, there are insufficient data to support a specific upper limit for phosphate intake. This review also proposes areas where additional research is needed in order to strengthen conclusions and recommendations regarding dietary phosphorus for cats

Experimental characterization of the fracture properties of pultruded GFRP structural elements

Pultruded Fiber Reinforced Polymers (FRPs) are a class of novel composite materials with remarkable strength (comparable or even greater to that of steel) and resistance to environmental effects. However, the strongly orthotropic behavior of these materials and the relatively high deformability and spatial variability in mechanical properties bring challenges to the widespread adoption of these elements in structural applications. To this end, the orientation and distribution of the fibers are the most influential parameters that affect both the ultimate strength and stiffness of the specimens. This work presents an experimental campaign conducted on GFRP specimens in uniaxial tension and 3-point bending; coupon specimens with three different fibers orientations (namely 0, 45, and 90 degrees) were tested to characterize the ultimate strength and failure modes Results of such experimental campaign are first presented, and detailed statistical measures of the so-obtained strength values are presented with the ultimate goal of characterizing the variability in mechanical properties in commercially available profiles

Effects of obesity, energy restriction and neutering on the faecal microbiota of cats

Surveys report that 25–57 % of cats are overweight or obese. The most evinced cause is neutering. Weight loss often fails; thus, new strategies are needed. Obesity has been associated with altered gut bacterial populations and increases in microbial dietary energy extraction, body weight and adiposity. This study aimed to determine whether alterations in intestinal bacteria were associated with obesity, energy restriction and neutering by characterising faecal microbiota using 16S rRNA gene sequencing in eight lean intact, eight lean neutered and eight obese neutered cats before and after 6 weeks of energy restriction. Lean neutered cats had a bacterial profile similar to obese rodents and humans, with a greater abundance (P<0·05) of Firmicutes and lower abundance (P <0·05) of Bacteroidetes compared with the other groups. The greater abundance of Firmicutes in lean neutered cats was due to a bloom in Peptostreptococcaceae. Obese cats had an 18 % reduction in fat mass after energy restriction (P<0·05). Energy reduction was concurrent with significant shifts in two low-abundance bacterial genera and trends in four additional genera. The greatest change was a reduction in the Firmicutes genus, Sarcina, from 4·54 to 0·65 % abundance after energy restriction. The short duration of energy restriction may explain why few bacterial changes were observed in the obese cats. Additional work is needed to understand how neutering, obesity and weight loss are related to changes in feline microbiota and how these microbial shifts affect host physiology

Nanoparticle Tracking Analysis for the Enumeration and Characterization of Mineralo-Organic Nanoparticles in Feline Urine

Urinary stone disease, particularly calcium oxalate, is common in both humans and cats. Calcifying nanoparticles (CNP) are spherical nanocrystallite material, and are composed of proteins (fetuin, albumin) and inorganic minerals. CNP are suggested to play a role in a wide array of pathologic mineralization syndromes including urolithiasis. We documented the development of a clinically relevant protocol to assess urinary CNP in 9 healthy cats consuming the same diet in a controlled environment using Nanoparticle Tracking Analysis (NTA®). NTA® is a novel method that allows for characterization of the CNP in an efficient, accurate method that can differentiate these particles from other urinary submicron particulates. The predominant nanoscale particles in feline urine are characteristic of CNP in terms of their size, their ability to spontaneously form under suitable conditions, and the presence of an outer layer that is rich in calcium and capable of binding to hydroxyapatite binders such as alendronate and osteopontin. The expansion of this particle population can be suppressed by the addition of citrate to urine samples. Further, compounds targeting exosomal surfaces do not label these particulates. As CNP have been associated with a number of significant urologic maladies, the method described herein may prove to be a useful adjunct in evaluating lithogenesis risk in mammals

Relevant but neglected habitat types by the Directive 92/43 EEC in southern Italy

The 92/43/EEC Habitats Directive is the main European Union legal tool concerning nature conservation. The habitat types listed in Annex I to the Directive are phytosociology-based. It is widely acknowledged that phytosociological analysis is a crucial approach for habitats characterization and for monitoring their conservation status. Based on bibliographic investigations and new field survey campaigns, a list of habitat types neglected by the Habitats Directive is here presented and described for southern Italy. In this paper, 8 new habitat types and 13 subtypes are proposed. For each of these proposed new habitat types, a wide range of information, including ecology, chorology, species composition, syntaxonomy, threats, and conservation status, is here provided. To supply more detailed phytogeographical and coenological information about the proposed new habitat types, distribution maps based on 10 x 10 km reference grids and phytosociological tables including unpublished releves were carried out