4,102 research outputs found
Phosphorylation of CENP-A on serine 7 does not control centromere function
CENP-A is the histone H3 variant necessary to specify the location of all eukaryotic centromeres via its CENP-A targeting domain and either one of its terminal regions. In humans, several post-translational modifications occur on CENP-A, but their role in centromere function remains controversial. One of these modifications of CENP-A, phosphorylation on serine 7, has been proposed to control centromere assembly and function. Here, using gene targeting at both endogenous CENP-A alleles and gene replacement in human cells, we demonstrate that a CENP-A variant that cannot be phosphorylated at serine 7 maintains correct CENP-C recruitment, faithful chromosome segregation and long-term cell viability. Thus, we conclude that phosphorylation of CENP-A on serine 7 is dispensable to maintain correct centromere dynamics and function
Simultaneous X-ray and optical spectroscopy of the Oef supergiant lambda Cep
Probing the structures of stellar winds is of prime importance for the
understanding of massive stars. Based on their optical spectral morphology and
variability, the stars of the Oef class have been suggested to feature
large-scale structures in their wind. High-resolution X-ray spectroscopy and
time-series of X-ray observations of presumably-single O-type stars can help us
understand the physics of their stellar winds. We have collected XMM-Newton
observations and coordinated optical spectroscopy of the O6Ief star lambda Cep
to study its X-ray and optical variability and to analyse its high-resolution
X-ray spectrum. We investigate the line profile variability of the He II 4686
and H-alpha emission lines in our time series of optical spectra, including a
search for periodicities. We further discuss the variability of the broadband
X-ray flux and analyse the high-resolution spectrum of lambda Cep using
line-by-line fits as well as a code designed to fit the full high-resolution
X-ray spectrum consistently. During our observing campaign, the He II 4686 line
varies on a timescale of ~18 hours. On the contrary, the H-alpha line profile
displays a modulation on a timescale of 4.1 days which is likely the rotation
period of the star. The X-ray flux varies on time-scales of days and could in
fact be modulated by the same 4.1 days period as H-alpha, although both
variations are shifted in phase. The high-resolution X-ray spectrum reveals
broad and skewed emission lines as expected for the X-ray emission from a
distribution of wind-embedded shocks. Most of the X-ray emission arises within
less than 2R* above the photosphere.Comment: Accepted for publication in Astronomy & Astrophysic
Building block libraries and structural considerations in the self-assembly of polyoxometalate and polyoxothiometalate systems
Inorganic metal-oxide clusters form a class of compounds that are unique in their topological and electronic versatility and are becoming increasingly more important in a variety of applications. Namely, Polyoxometalates (POMs) have shown an unmatched range of physical properties and the ability to form structures that can bridge several length scales. The formation of these molecular clusters is often ambiguous and is governed by self-assembly processes that limit our ability to rationally design such molecules. However, recent years have shown that by considering new building block principles the design and discovery of novel complex clusters is aiding our understanding of this process. Now with current progress in thiometalate chemistry, specifically polyoxothiometalates (POTM), the field of inorganic molecular clusters has further diversified allowing for the targeted development of molecules with specific functionality. This chapter discusses the main differences between POM and POTM systems and how this affects synthetic methodologies and reactivities. We will illustrate how careful structural considerations can lead to the generation of novel building blocks and further deepen our understanding of complex systems
The EDELWEISS Experiment : Status and Outlook
The EDELWEISS Dark Matter search uses low-temperature Ge detectors with heat
and ionisation read- out to identify nuclear recoils induced by elastic
collisions with WIMPs from the galactic halo. Results from the operation of 70
g and 320 g Ge detectors in the low-background environment of the Modane
Underground Laboratory (LSM) are presented.Comment: International Conference on Dark Matter in Astro and Particle Physics
(Dark 2000), Heidelberg, Germany, 10-16 Jul 2000, v3 minor revision
Spitzer view on the evolution of star-forming galaxies from z=0 to z~3
We use a 24 micron selected sample containing more than 8,000 sources to
study the evolution of star-forming galaxies in the redshift range from z=0 to
z~3. We obtain photometric redshifts for most of the sources in our survey
using a method based on empirically-built templates spanning from ultraviolet
to mid-infrared wavelengths. The accuracy of these redshifts is better than 10%
for 80% of the sample. The derived redshift distribution of the sources
detected by our survey peaks at around z=0.6-1.0 (the location of the peak
being affected by cosmic variance), and decays monotonically from z~1 to z~3.
We have fitted infrared luminosity functions in several redshift bins in the
range 0<z<~3. Our results constrain the density and/or luminosity evolution of
infrared-bright star-forming galaxies. The typical infrared luminosity (L*)
decreases by an order of magnitude from z~2 to the present. The cosmic star
formation rate (SFR) density goes as (1+z)^{4.0\pm0.2} from z=0 to z=0.8. From
z=0.8 to z~1.2, the SFR density continues rising with a smaller slope. At
1.2<z<3, the cosmic SFR density remains roughly constant. The SFR density is
dominated at low redshift (z<0.5) by galaxies which are not very luminous in
the infrared (L_TIR<1.e11 L_sun, where L_TIR is the total infrared luminosity,
integrated from 8 to 1000 micron). The contribution from luminous and
ultraluminous infrared galaxies (L_TIR>1.e11 L_sun) to the total SFR density
increases steadily from z~0 up to z~2.5, forming at least half of the
newly-born stars by z~1.5. Ultraluminous infrared galaxies (L_TIR>1.e12 L_sun)
play a rapidly increasing role for z>~1.3.Comment: 28 pages, 17 figures, accepted for publication in Ap
Pleiades or Not? Resolving the Status of the Lithium-rich M Dwarfs HHJ 339 and HHJ 430
Oppenheimer et al. discovered two M5 dwarfs in the Pleiades with nearly primordial lithium. These stars are not low enough in mass to represent the leading edge of the lithium depletion boundary at Pleiades age (~125 Myr). A possible explanation for the enhanced lithium in these stars is that they are actually not members of the Pleiades but instead are members of a younger moving group seen in projection toward the Pleiades. We have used data from Gaia DR2 to confirm that these two stars, HHJ 339 and HHJ 430, are indeed not members of the Pleiades. Based on their space motions, parallaxes, and positions in a Gaia-based color–magnitude diagram, it is probable that these two stars are about 40 parsecs foreground to the Pleiades and have ages of ~25 Myr. Kinematically they are best matched to the 32 Ori moving group
Background studies for the EDELWEISS dark matter experiment
The EDELWEISS-II collaboration has completed a direct search for WIMP dark
matter using cryogenic Ge detectors (400 g each) and 384 kgdays of
effective exposure. A cross-section of pb is excluded at
90% C.L. for a WIMP mass of 85 GeV. The next phase, EDELWEISS-III, aims to
probe spin-independent WIMP-nucleon cross-sections down to a few
pb. We present here the study of gamma and neutron background
coming from radioactive decays in the set-up and shielding materials. We have
carried out Monte Carlo simulations for the completed EDELWEISS-II setup with
GEANT4 and normalised the expected background rates to the measured
radioactivity levels (or their upper limits) of all materials and components.
The expected gamma-ray event rate in EDELWEISS-II at 20-200 keV agrees with the
observed rate of 82 events/kg/day within the uncertainties in the measured
concentrations. The calculated neutron rate from radioactivity of 1.0-3.1
events (90% C.L.) at 20-200 keV in the EDELWEISS-II data together with the
expected upper limit on the misidentified gamma-ray events (), surface
betas (), and muon-induced neutrons (), do not contradict 5
observed events in nuclear recoil band. We have then extended the simulation
framework to the EDELWEISS-III configuration with 800 g crystals, better
material purity and additional neutron shielding inside the cryostat. The
gamma-ray and neutron backgrounds in 24 kg fiducial mass of EDELWEISS-III have
been calculated as 14-44 events/kg/day and 0.7-1.4 events per year,
respectively. The results of the background studies performed in the present
work have helped to select better purity components and improve shielding in
EDELWEISS-III to further reduce the expected rate of background events in the
next phase of the experiment.Comment: 15 pages, 9 figures, to be published in Astroparticle Physic
Axion searches with the EDELWEISS-II experiment
We present new constraints on the couplings of axions and more generic
axion-like particles using data from the EDELWEISS-II experiment. The EDELWEISS
experiment, located at the Underground Laboratory of Modane, primarily aims at
the direct detection of WIMPs using germanium bolometers. It is also sensitive
to the low-energy electron recoils that would be induced by solar or dark
matter axions. Using a total exposure of up to 448 kg.d, we searched for
axion-induced electron recoils down to 2.5 keV within four scenarios involving
different hypotheses on the origin and couplings of axions. We set a 95% CL
limit on the coupling to photons GeV in
a mass range not fully covered by axion helioscopes. We also constrain the
coupling to electrons, , similar to the more
indirect solar neutrino bound. Finally we place a limit on , where is the
effective axion-nucleon coupling for Fe. Combining these results we
fully exclude the mass range keV for DFSZ axions and
keV for KSVZ axions
Measurements of , , , and proton production in proton-carbon interactions at 31 GeV/ with the NA61/SHINE spectrometer at the CERN SPS
Measurements of hadron production in p+C interactions at 31 GeV/c are
performed using the NA61/ SHINE spectrometer at the CERN SPS. The analysis is
based on the full set of data collected in 2009 using a graphite target with a
thickness of 4% of a nuclear interaction length. Inelastic and production cross
sections as well as spectra of , , p, and are
measured with high precision. These measurements are essential for improved
calculations of the initial neutrino fluxes in the T2K long-baseline neutrino
oscillation experiment in Japan. A comparison of the NA61/SHINE measurements
with predictions of several hadroproduction models is presented.Comment: v1 corresponds to the preprint CERN-PH-EP-2015-278; v2 matches the
final published versio
Multiplicity and transverse momentum fluctuations in inelastic proton-proton interactions at the CERN Super Proton Synchrotron
Measurements of multiplicity and transverse momentum fluctuations of charged
particles were performed in inelastic p+p interactions at 20, 31, 40, 80 and
158 GeV/c beam momentum. Results for the scaled variance of the multiplicity
distribution and for three strongly intensive measures of multiplicity and
transverse momentum fluctuations \$\Delta[P_{T},N]\$, \$\Sigma[P_{T},N]\$ and
\$\Phi_{p_T}\$ are presented. For the first time the results on fluctuations
are fully corrected for experimental biases. The results on multiplicity and
transverse momentum fluctuations significantly deviate from expectations for
the independent particle production. They also depend on charges of selected
hadrons. The string-resonance Monte Carlo models EPOS and UrQMD do not describe
the data. The scaled variance of multiplicity fluctuations is significantly
higher in inelastic p+p interactions than in central Pb+Pb collisions measured
by NA49 at the same energy per nucleon. This is in qualitative disagreement
with the predictions of the Wounded Nucleon Model. Within the statistical
framework the enhanced multiplicity fluctuations in inelastic p+p interactions
can be interpreted as due to event-by-event fluctuations of the fireball energy
and/or volume.Comment: 18 pages, 12 figure
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