Quantification of phytic acid in grains

This report describes the validation of a cost effective method for quantifying phytic acid in grains, namely, rice and wheat, using UV/Vis spectroscopy. Background information describing phytic acid and its impact on human biological systems and hence the importance of its analysis is included in this report. The validation method involved a range of tests to determine accuracy, precision and reproducibility of the method. Multiple sample matrices were used including standards and spiked samples as described in the validation plan and criteria in Appendix 2. The method employed a commercially available assay kit from Megazyme® and was found to give accurate reliable data according to the performance characteristics attained. This method also has the potential for transfer to laboratories with limited resources, in particular developing countries. It is applicable to survey scale and small batch analysis owing to its relatively low start up and running costs, fast analysis time and ease of instrument set up for each analytical batch compared to established methods using ion chromatography

Determining constraints imposed by salt fabrics on the morphology of solution-mined energy storage cavities, through dissolution experiments using brine and seawater in halite

Large-scale compressed air energy storage facilities offer one solution to the UK's energy demands, using solution-mined caverns in salt lithologies. For optimum gas storage efficiency, cavern geometry should ideally be smooth: spherical to cylindrical with a circular cross-section. However, such caverns are often irregular with marked asymmetry or ellipticity, and although the reasons for non-circular cross-sections developing during solution mining in some caverns can be related to, for example, the presence of interbedded lithologies, in other instances they are not fully understood. Cavities from dissolution experiments using five main end-member salt facies fabrics from the Triassic Preesall and Northwich Halite formations have been assessed to determine factors affecting cavity geometry, formation and variability in dissolution behaviour. Identical sets of experiments were performed on each fabric type, using two solution concentration strengths: brine and synthetic seawater. Comparison of experimental results using a combination of analytical and imaging techniques shows the extent to which the salt fabric and enhancement of features within the salt influence the resulting dissolution cavity. Observations show a visible increase in micropores within the adjacent halite matrix following dissolution. Smaller-scale features provide further insights into the dissolution processes, and salt fabric behaviour under different dissolution conditions. Supplementary material: A detailed description of methods is available at https://doi.org/10.6084/m9.figshare.c.428245

Herpes-Virus Infection in Patients with Langerhans Cell Histiocytosis: A Case-Controlled Sero-Epidemiological Study, and In Situ Analysis

BACKGROUND: Langerhans cell histiocytosis (LCH) is a rare disease that affects mainly young children, and which features granulomas containing Langerhans-type dendritic cells. The role of several human herpesviruses (HHV) in the pathogenesis of LCH was suggested by numerous reports but remains debated. Epstein-barr virus (EBV, HHV-4), & Cytomegalovirus (CMV, HHV-5) can infect Langerhans cells, and EBV, CMV and HHV-6 have been proposed to be associated with LCH based on the detection of these viruses in clinical samples. METHODOLOGY: We have investigated the prevalence of EBV, CMV and HHV-6 infection, the characters of antibody response and the plasma viral load in a cohort of 83 patients and 236 age-matched controls, and the presence and cellular localization of the viruses in LCH tissue samples from 19 patients. PRINCIPAL FINDINGS: The results show that prevalence, serological titers, and viral load for EBV, CMV and HHV-6 did not differ between patients and controls. EBV was found by PCR in tumoral sample from 3/19 patients, however, EBV small RNAs EBERs -when positive-, were detected by in situ double staining in bystander B CD20+ CD79a+ lymphocytes and not in CD1a+ LC. HHV-6 genome was detected in the biopsies of 5/19 patients with low copy number and viral Ag could not be detected in biopsies. CMV was not detected by PCR in this series. CONCLUSIONS/SIGNIFICANCE: Therefore, our findings do not support the hypothesis of a role of EBV, CMV, or HHV-6 in the pathogenesis of LCH, and indicate that the frequent detection of Epstein-barr virus (EBV) in Langerhans cell histiocytosis is accounted for by the infection of bystander B lymphocytes in LCH granuloma. The latter observation can be attributed to the immunosuppressive micro environment found in LCH granuloma

Bioavailability of copper, zinc and arsenic species in runoff waters from contrasted fields in a Mediterranean vineyard catchment

Poster *INRA UMR Sol et Environnement Montpellier (FRA) Diffusion du document : INRA UMR Sol et Environnement Montpellier (FRA)International audienc