Subcellular distribution and expression of cofilin and ezrin in human colon adenocarcinoma cell lines with different metastatic potential

The dynamic reorganization of the actin cytoskeleton is regulated by a number of actin binding proteins (ABPs). Four human colon adenocarcinoma cell lines – parental and three selected sublines, which differ in motility and metastatic potential, were used to investigate the expression level and subcellular localization of selected ABPs. Our interest was focused on cofilin and ezrin. These proteins are essential for cell migration and adhesion. The data received for the three more motile adenocarcinoma sublines (EB3, 3LNLN, 5W) were compared with those obtained for the parental LS180 adenocarcinoma cells and fibroblastic NRK cells. Quantitative densitometric analysis and confocal fluorescence microscopy were used to examine the expression levels and subcellular distribution of the selected ABPs. Our data show distinct increase in the level of cofilin in adenocarcinoma cells accompanied by the reduction of inactive phosphorylated form of cofilin. In more motile cells, cofilin was accumulated at cellular periphery in co-localization with actin filaments. Furthemore, we indicated translocation of ezrin towards the cell periphery within more motile cells in comparison with NRK and parental adenocarcinoma cells

VLBI study of maser kinematics in high-mass SFRs. II. G23.01-0.41

The present paper focuses on the high-mass star-forming region G23.01-0.41. Methods: Using the VLBA and the EVN arrays, we conducted phase-referenced observations of the three most powerful maser species in G23.01-0.41: H2O at 22.2 GHz (4 epochs), CH3OH at 6.7 GHz (3 epochs), and OH at 1.665 GHz (1 epoch). In addition, we performed high-resolution (> 0".1), high-sensitivity (< 0.1 mJy) VLA observations of the radio continuum emission from the HMC at 1.3 and 3.6 cm. Results: We have detected H2O, CH3OH, and OH maser emission clustered within 2000 AU from the center of a flattened HMC, oriented SE-NW, from which emerges a massive 12CO outflow, elongated NE-SW, extended up to the pc-scale. Although the three maser species show a clearly different spatial and velocity distribution and sample distinct environments around the massive YSO, the spatial symmetry and velocity field of each maser specie can be explained in terms of expansion from a common center, which possibly denotes the position of the YSO driving the maser motion. Water masers trace both a fast shock (up to 50 km/s) closer to the YSO, powered by a wide-angle wind, and a slower (20 km/s) bipolar jet, at the base of the large-scale outflow. Since the compact free-free emission is found offset from the putative location of the YSO along a direction consistent with that of the maser jet axis, we interpret the radio continuum in terms of a thermal jet. The velocity field of methanol masers can be explained in terms of a composition of slow (4 km/s in amplitude) motions of radial expansion and rotation about an axis approximately parallel to the maser jet. Finally, the distribution of line of sight velocities of the hydroxyl masers suggests that they can trace gas less dense (n(H2) < 10^6 cm^-3) and more distant from the YSO than that traced by the water and methanol masers, which is expanding toward the observer. (Abridged)Comment: 23 pages, 8 figures, 4 tables, accepted by Astronomy and Astrophysic

12.2-GHz methanol maser MMB follow-up catalogue - I. Longitude range 330 to 10 degrees

We present a catalogue of 12.2-GHz methanol masers detected towards 6.7-GHz methanol masers observed in the unbiased Methanol Multibeam (MMB) survey in the longitude range 330\circ (through 360\circ) to 10\circ. This is the first portion of the catalogue which, when complete, will encompass all of the MMB detections. We report the detection of 184 12.2-GHz sources towards 400 6.7-GHz methanol maser targets, equating to a detection rate of 46 per cent. Of the 184 12.2-GHz detections, 117 are reported here for the first time. We draw attention to a number of 'special' sources, particularly those with emission at 12.2-GHz stronger than their 6.7-GHz counterpart and conclude that these unusual sources are not associated with a specific evolutionary stage.Comment: accepted to MNRAS 21 Dec 201

12.2-GHz methanol maser MMB follow-up catalogue - II. Longitude range 186 to 330 degrees

We present the second portion of a catalogue of 12.2-GHz methanol masers detected towards 6.7-GHz methanol masers observed in the unbiased Methanol Multibeam (MMB) Survey. Using the Parkes radio telescope we have targeted all 207 6.7-GHz methanol masers in the longitude range 186 to 330 degrees for 12.2-GHz counterparts. We report the detection of 83 12.2-GHz methanol masers, and one additional source which we suspect is thermal emission, equating to a detection rate of 40 per cent. Of the 83 maser detections, 39 are reported here for the first time. We discuss source properties, including variability and highlight a number of unusual sources. We present a list of 45 candidates that are likely to harbor methanol masers in the 107.0-GHz transition.Comment: Accepted MNRAS 19 July 201

Towards the possibility to combine LOFAR and GNSS measurements to sense ionospheric irregularities

Inhomogeneities within the ionospheric plasma density affect trans-ionospheric radio signals, causing radio wave scintillation in the amplitude and phase of the signals. The amount of scintillation induced by ionospheric irregularities typically decreases with the radio wave frequency. As the ionosphere affects a variety of technological systems (e.g., civil aviation, financial operations) as well as low-frequency radio astronomy observations, it is important to detect and monitor iono- spheric effects with higher accuracy than currently available. Here, a novel methodology for the detection and characterization of ionospheric irregularities is established on the basis of LOFAR scintillation measurements at VHF that takes into account of the lack of ergodicity in the intensity fluctuations induced by scintillation. The methodology estimates the S 4 scintillation index originating from irregularities with spatial scales in the inertial sub-range of electron density fluctuations in the ionosphere. The methodology is illustrated by means of observations that were collected through the Polish LOFAR stations located in Bałdy, Borówiec and Łazy: its validation was carried out by comparing LOFAR VHF scintillation observations with independent GNSS observations that were collected through a high-rate receiver located near the LOFAR station in Bałdy as well as through geodetic receivers from the Polish ASG-EUPOS network. Two case stud- ies are presented: 31 March 2017 and 28 September 2017. The comparison between LOFAR S4 observations and independent ionospheric measurements of both scintillation and rate of change of TEC from GNSS reveals that the sensitivity of LOFAR and GNSS to ionospheric structures is different as a consequence of the frequency dependency of radio wave scintillation. Furthermore, it can be noticed that observations of LOFAR VHF scintillation can be utilised to detect plasma structures forming in the mid-latitude ionosphere, including electron density gradients occurring over spatial scales that are not necessarily detected through traditional GNSS measurements: the detection of all spatial scales is important for a correct monitoring and modelling of ionospheric processes. Hence, the different sensitivity of LOFAR to ionospheric structures, in addition to traditional GNSS ionospheric measurements, allows to expand the knowledge of ionospheric processes

Interferometric imaging of the type IIIb and U radio bursts observed with LOFAR on 22 August 2017

Context. The Sun is the source of different types of radio bursts that are associated with solar flares, for example. Among the most frequently observed phenomena are type III solar bursts. Their radio images at low frequencies (below 100 MHz) are relatively poorly studied due to the limitations of legacy radio telescopes. Aims. We study the general characteristics of types IIIb and U with stria structure solar radio bursts in the frequency range of 20-80 MHz, in particular the source size and evolution in different altitudes, as well as the velocity and energy of electron beams responsible for their generation. Methods. In this work types IIIb and U with stria structure radio bursts are analyzed using data from the LOFAR telescope including dynamic spectra and imaging observations, as well as data taken in the X-ray range (GOES and RHESSI satellites) and in the extreme ultraviolet (SDO satellite). Results. In this study we determined the source size limited by the actual shape of the contour at particular frequencies of type IIIb and U solar bursts in a relatively wide frequency band from 20 to 80 MHz. Two of the bursts seem to appear at roughly the same place in the studied active region and their source sizes are similar. It is different in the case of another burst, which seems to be related to another part of the magnetic field structure in this active region. The velocities of the electron beams responsible for the generation of the three bursts studied here were also found to be different

The trace fossil Lepidenteron lewesiensis: a taphonomic window on diversity of Late Cretaceous fishes

The trace fossil Lepidenteron lewesiensis (Mantell 1822) provides an exceptional taphonomic window to diversity of fishes as shown for the Upper Cretaceous of Poland, in the Middle Turonian–Lower Maastrichtian deposits of the Opole Trough, Miechów Trough, Mazury-Podlasie Homocline, and SE part of the Border Synclinorium. Lepidenteron lewesiensis is an unbranched burrow lined with small fish scales and bones, without a constructed wall. It contains scales, vertebrae, and bones of the head belonging to ten taxa of teleostean fishes: two undetermined teleosteans, six undetermined Clupeocephala, one Dercetidae, and one undetermined euteleostean. The preservation of fish remains suggests that fishes were pulled down into the burrow by an animal, probably by eunicid polychaetes.Das Spurenfossil Lepidenteron lewesiensis (Mantell 1822) ermöglicht einen biostratinomischen Einblick in die Diversität von Fischen, wie Fossilmaterial aus der Oberkreide von Polen zeigt. Es stammt aus dem Mittelturonium bis Untermaastrichtium des südöstlichen Abschnittes der Grenz-Synklinale, dem Opolen-Trog, dem Miechów-Trog und der Masuren-Podlachien-Homoklinale. L. lewesiensis ist ein unverzweigter Grabgang ohne ausgekleidete Wände, dessen Ränder von kleinen Fischschuppen und—knochen gebildet werden. Diese setzen sich aus Schuppen, Wirbel und Schädelknochen von zehn Teleostei-Taxa zusammen und zwar aus zwei unbestimmte Teleosteer, sechs unbestimmten Clupeocephala, einem Dercetidae und einem unbestimmten Euteleostei. Die Erhaltung der Fischüberreste deutet darauf hin, dass die Fische von einem Tier, wahrscheinlich einem Polychaeten der Familie Eunicidae, in den Bau gezogen wurden.We are very grateful to Dr. Lionel Cavin (Geneva) and the anonymous reviewer for constructive comments on an earlier version of the manuscript. Additional support was provided by the Jagiellonian University (DS funds), National Science Center (Grant Number: PRO-2011/01/N/ST10/07717), and the Laboratory of Geology (University of Lodz) BSt Grant No. 560/844. We are grateful to Dr. Johann Egger (Wien) and Kilian Eichenseer M.Sc. (Erlangen) for help with translating the abstract into German. We are grateful to Dr. Ursula Göhlich (Wien) for access to the Dercetis specimen

Predicting rolling resistance of farm tractor wheels running on soil

W pracy przedstawiono ocenę dokładności modelu opona-gleba zastosowanego do prognozowania oporu toczenia kół wybranych ciągników rolniczych poruszających się po spulchnionej glebie lekkiej. Wykazano, że prognozowanie oporu toczenia badanych ciągników za pomocą tego modelu jest dokonywane z dokładnością obarczoną błędem mniejszym od 11%. Stwierdzono, że dokładność prognozowania oporu toczenia wybranych pojedynczych kół prowadzących i kół napędowych jest obarczona błędami względnymi wynoszącymi poniżej 18%. Z badań empirycznych wynika, że opór toczenia kół prowadzących, o znacznie mniejszych wymiarach od kół napędowych, stanowi bardzo duży udział w oporze toczenia całego ciągnika wynosząc od 40 do 50%.The paper presents evaluation of accuracy of the tyre-soil model employed to predict rolling resistance for wheels of selected farm tractors running on a scarified light soil. It has been proven that predicting rolling resistance for the examined tractors with this model is carried out with accuracy burdened with error under 11%. It has been observed that the accuracy of rolling resistance predicting for selected single leading wheels and drive wheels is burdened with relative errors under 18%. Empirical research proves that rolling resistance of leading wheels, which are much smaller than drive wheels, has very high share in rolling resistance of the whole tractor, ranging from 40 to 50%