Aplikasi Abu Sekam Pada Padi Gogo (Oryza Sativa L.) Terhadap Kandungan Silikat Dan Prolin Daun Serta Amilosa Dan Protein Biji

Tujuan dari penelitian ini adalah untuk mengetahui pengaruh aplikasi abu sekam terhadap kandungan prolin dan silikat daun serta kualitas hasil yaitu protein dan amilosa biji padi gogo dan hubungan korelasi antar keempat komponen tersebut pada kondisi pertanaman 80 persen kapasitas lapang pada skala pot. Penelitian dilakukan di polibag dalam screen house Fakultas Pertanian Unsoed dengan menggunakan rancangan acak kelompok pola faktorial dengan faktor varietas (Situ patenggang, Limboto, Towuti, Batutegi dan Aek sibundong) dan faktor abu sekam (0, 2, 4, 6 t/ha), diulang tiga kali. Hasil penelitian menunjukan bahwa pemberian abu sekam dosis 2âˆ\u276 t/ha mampu meningkatkan kandungan silikat daun antara 18,49âˆ\u2729,43% dan menurunkan kandungan amilosa biji pada lima varietas sekitar 4,19âˆ\u276,92%. Pemberian abu sekam dosis 2âˆ\u276 t/ha mampu meningkatkan kandungan prolin daun antara 27,56âˆ\u2770,63% dan protein biji antara 2,35âˆ\u2716,71%. Antarvarietas menunjukan bahwa kandungan prolin tertinggi dihasilkan oleh varietas Batu tegi 18,58 persen dan protein biji pada varietas Situ patenggang 9,55%. Terdapat korelasi antar karakter fisiologis yaitu antara silikat-prolin (0,62) dan kandungan protein-amilosa biji (-0,78)

Wisteria vein mosaic virus detected for the first time in Iran from an unknown host by analysis of aphid vectors

The development of reverse transcription-polymerase chain reaction using degenerate primers against conserved regions of most potyviral genomes enabled sampling of the potyvirome. However, these assays usually involve sampling potential host plants, but identifying infected plants when they are asymptomatic is challenging, and many plants, especially wild ones, contain inhibitors to DNA amplification. We used an alternative approach which utilized aphid vectors and indicator plants to identify potyviruses capable of infecting common bean (Phaseolus vulgaris). Aphids were collected from a range of asymptomatic leguminous weeds and trees in Iran, and transferred to bean seedlings under controlled conditions. Bean plants were tested serologically for potyvirus infections four-weeks post-inoculation. The serological assay and symptomatology together indicated the presence of one potyvirus, and symptomology alone implied the presence of an unidentified virus. The partial genome of the potyvirus, encompassing the complete coat protein gene, was amplified using generic potyvirus primers. Sequence analysis of the amplicon confirmed the presence of an isolate of Wisteria vein mosaic virus (WVMV), a virus species not previously identified from Western Asia. Phylogenetic analyses of available WVMV sequences categorized them into five groups: East Asian-1 to 3, North American and World. The Iranian isolate clustered with those in the World group. Multiple sequence alignment indicated the presence of some genogroup-specific amino acid substitutions among the isolates studied. Chinese isolates were sister groups of other isolates and showed higher nucleotide distances as compared with the others, suggesting a possible Eastern-Asian origin of WVMV, the main region where Wisteria might have originated

Pengaruh Aplikasi Pseudomonas Fluorescens P60 Terhadap Mutu Patologis, Mutu Fisiologis, Dan Pertumbuhan Bibit Padi Ir 64

Effect of Pseudomonas fluorescens P60 on pathological and physiological quality and growth of rice IR 64 seedlings. The research objectives were (1) detection and identification of seed-borne pathogens of IR 64 rice, (2) testing Pseudomonas fluorescents P60 in inhibiting the in vitro growth of seed-borne pathogens colonies, (3) testing P. fluorescents P60 for pathological and physiological seed quality, and (4) testing P. fluorescents P60 on the growth of seedlings in the greenhouse. The results showed that some seed-borne pathogens can be found both on farmers' IR 64 rice and factory's; they were Aspergillus flavus, Alternaria padwickii, Pseudomonas glumae, and P. syringae. Application of P. flourescens P60 was able to inhibit the in vitrogrowth of colonies of all seed-borne pathogens, except P. syringae. Related to pathological quality, the effect of P. flourescens P60 on percentage of seed-borne pathogens attack did not significantly different from that of benomil but smaller than distilled water. On the physiological quality of seeds, treatment of P. flourescens P60 has the same effect with benomil and distilled water, with germination rate was more than 80%. In the greenhouse study,treatment of seed immersion time in P. flourescens P60 suspension showed that the effect of immersion time as long as15 minutes and 25 minutes on seedling height, root length, and seedling dry weightdid not significantly different. were. However, 25 minutes immersion time resulted in fresh seedling weight and root dry weight higher than that of 15 minutes immersion time

Exploration and remediation ability test of indigenous bacteria from rice field pemalang regency on lead (Pb) contamined soil

The research aims to obtain isolates and determine the morphological characteristics of bacteria that are resistant to Lead (Pb) contamination. The materials and tools used were soil from conventional rice farming land, Nutrient Agar (NA) medium, alcohol 70%, aquades, PbNO3 solution, aluminum foil, cling wrap, heat-resistant plastic, spiritus, physiological solution of 0.85% NaCl, autoclave, test tubes, petri dishes, erlenmeyer flasks, measuring cylinder, beaker glass, laminar air flow (LAF), shaker, magnetic stirrer, micropipette, refrigerator, analytical balance, ose needle, pH meter, vortex, ice box, shovel , tube racks, bunsen, stationery, labels, and other supporting materials and equipment. This research is a sampling research and continued with laboratory tests including bacterial isolation, colony count calculation and macromorphological characterization. The data obtained from morphological observations are presented in the form of descriptions, while quantitative data are presented in the form of numbers and are used as primary data. The results of isolating bacteria with NA medium added with 5 ppm lead, there are five bacterial isolates were selected, namely KMPb O, KT1Pb C, KT2Pb H, KB1Pb H, and KB2Pb K. Based on the TPC (Total Plate Count) test with a range of 30-300 colonies, the number of bacterial colonies ranged from 10,45 x 106 – 28,9 x 106 CFU/ml. The colony morphological characteristics of the five bacterial isolates were dominated by round in shape, smooth texture and flat elevation

checklist and a key to the Iranian species

In this study, we reported the presence of six zerconid species belonging to two genera (Prozercon and Zercon) which were collected between May 2014 and November 2015 from East Azerbaijan and Lorestan Provinces of Iran. Of these, Prozercon bircanae, Zercon colligans, Z. notabilis, and Z. turcicus are newly recorded for the Iran acarofauna. Some known zerconid mites are recorded from new localities in the country. In addition, a checklist, distribution maps, and a key to the species of the family Zerconidae known from Iran were given

from Mazandaran Province, Iran, with a review of the Iranian species

A new species of Olopachys Berlese, Olopachys (Olopachys) magnoexudatus sp. nov., is described herein, based on female specimens collected from soil and litter in the natural forests of Behshahr, Mazandaran Province, Iran. All previous records of Olopachys species in Iran are reviewed and discussed, and an identification key to the five Iranian species is provided.C1 [Moghimi, Fatemeh; Ahadiyat, Ali] Islamic Azad Univ, Dept Plant Protect, Sci & Res Branch, Tehran, Iran.[Kiadaliri, Hadi] Islamic Azad Univ, Dept Forest Range & Watershed Management, Sci & Res Branch, Tehran, Iran.[Karaca, Mehmet] Pamukkale Univ, Dept Elect & Automat, Denizli Vocat Sch Tech Sci, Denizli, Turkey

PENGARUH APLIKASI PSEUDOMONAS FLUORESCENS P60 TERHADAP MUTU PATOLOGIS, MUTU FISIOLOGIS, DAN PERTUMBUHAN BIBIT PADI IR 64

Effect of Pseudomonas fluorescens P60 on pathological and physiological quality and growth of rice IR 64 seedlings. The research objectives were (1) detection and identification of seed-borne pathogens of IR 64 rice, (2) testing Pseudomonas fluorescents P60 in inhibiting the in vitro growth of seed-borne pathogens colonies, (3) testing P. fluorescents P60 for pathological and physiological seed quality, and (4) testing P. fluorescents P60 on the growth of seedlings in the greenhouse. The results showed that some seed-borne pathogens can be found both on farmers' IR 64 rice and factory's; they were Aspergillus flavus, Alternaria padwickii, Pseudomonas glumae, and P. syringae. Application of P. flourescens P60 was able to inhibit the in vitrogrowth of colonies of all seed-borne pathogens, except P. syringae. Related to pathological quality, the effect of P. flourescens P60 on percentage of seed-borne pathogens attack did not significantly different from that of benomil but smaller than distilled water. On the physiological quality of seeds, treatment of P. flourescens P60 has the same effect with benomil and distilled water, with germination rate was more than 80%. In the greenhouse study,treatment of seed immersion time in P. flourescens P60 suspension showed that the effect of immersion time as long as15 minutes and 25 minutes on seedling height, root length, and seedling dry weightdid not significantly different. were. However, 25 minutes immersion time resulted in fresh seedling weight and root dry weight higher than that of 15 minutes immersion time

Phoretic uropodine mites (Acari: Mesostigmata) associated with the red palm weevil, Rhynchophorus ferrugineus (Coleoptera: Curculionidae) in Iran

During an investigation on phoretic mite associates of the red palm weevil, Rhynchophorus ferrugineus, in Sistan and Balouchestan Province of Iran, two uropodine species were collected and identified as Centrouropoda almerodai (Uropodidae) and Uroobovella marginata (Dinychidae). This is the first record of the genus Centrouropoda from Iran and the first record of phoretic mites associated with this weevil from the country