Coding-Complete Genome Sequence of a Black Queen Cell Virus Isolate from Honey Bees (Apis mellifera) in Italy

In this study, we documented the complete coding genome sequence of a Black queen cell virus (BQCV) isolate from honey bees in Italy. This genome sequence illustrates a high similarity with other BQCV isolates reported worldwide and could provide insights into BQCV genome phylogeny and divergence

Climate variability during MIS 20–18 as recorded by alkenone-SST and calcareous plankton in the Ionian Basin (central Mediterranean)

This study shows the first Mediterranean high-resolution record of alkenone-derived sea surface temperature (SST) in the marine sediments outcropping at the Ideale section (IS) (southern Italy, central Mediterranean) from late marine isotope stage (MIS) 20 - through early MIS 18. The SST pattern evidences glacial-interglacial up to submillennial-scale temperature variation, with lower values (~13 °C) in late MIS 20 and substage 19b, and higher values (up to 21 °C) in MIS 19c and in the interstadials of MIS 19a. The SST data are combined with the new calcareous plankton analysis and the available, chronologically well-constrained carbon and oxygen isotope records in the IS. The multi-proxy approach, together with the location of the IS near the Italian coasts, the lower circalittoral-upper bathyal depositional setting, and high sedimentation rate allow to document long-and short-term paleoenvironmental modifications (sea level, rainfall, inorganic/organic/fresh water input to the basin), as a response to regional and global climate changes. The combined proxies reveal the occurrence of a terminal stadial event in late MIS 20 (here Med-HTIX), and warm-cold episodes (here Med-BATIX and Med-YDTIX) during Termination IX (TIX), which recall those that occurred through the last termination (TI). During these periods and the following ghost sapropel layer (insolation cycle 74, 784 ka) in the early MIS 19, high frequency internal changes are synchronously recorded by all proxies. The substage MIS 19c is warm but quite unstable, with several episodes of paleoenvironmental changes, associated with fluctuating tropical-subtropical water inflow through the Gibraltar Strait, variations of the cyclonic regime in the Ionian basin, and the southward shift of westerly winds and winter precipitation over southern Europe and Mediterranean basin. Three high-amplitude millennial-scale oscillations in the patterns of SST and calcareous plankton key taxa during MIS 19a are interpreted as linked to changes in temperature as well as in salinity due to periodical water column stratification and mixing. The main processes involved in the climate variability include changes in oceanographic exchanges through the Gibraltar Strait during modulations of Atlantic meridional overturning circulation and/or variations in atmospheric dynamics related to the influence of westerly and polar winds acting in the paleo-Ionian basin. A strong climate teleconnection between the North Atlantic and Mediterranean is discussed, and a prominent role of atmospheric processes in the central Mediterranean is evidenced by comparing data sets at the IS with Italian and extra-Mediterranean marine and terrestrial records

What Are the Barriers and Motivators to Exercise in 50-65 Year-Old Adults?

Introduction. The benefit of exercise in adults has been well established. Research has demonstrated improved cardiovascular health, decreased bone fractures, and increased mental capacity. While the benefits of exercise has clearly been demonstrated, personal barriers to exercise are yet to be fully elucidated. Thus, in collaboration with the YMCA, this study aimed to clarify barriers to exercise in 50-65 year-old adults.https://scholarworks.uvm.edu/comphp_gallery/1085/thumbnail.jp

An optimized protocol for assessment of sputum macrorheology in health and muco-obstructive lung disease

Background: Airway mucus provides important protective functions in health and abnormal viscoelasticity is a hallmark of muco-obstructive lung diseases such as cystic fibrosis (CF). However, previous studies of sputum macrorheology from healthy individuals and patients with CF using different experimental protocols yielded in part discrepant results and data on a systematic assessment across measurement settings and conditions remain limited. Objectives: The aim of this study was to develop an optimized and reliable protocol for standardized macrorheological measurements of airway mucus model systems and native human sputum from healthy individuals and patients with muco-obstructive lung disease. Methods: Oscillatory rheological shear measurements were performed using bovine submaxillary mucin (BSM) at different concentrations (2% and 10% solids) and sputum samples from healthy controls (n = 10) and patients with CF (n = 10). Viscoelastic properties were determined by amplitude and frequency sweeps at 25°C and 37°C with or without solvent trap using a cone-plate geometry. Results: Under saturated atmosphere, we did not observe any temperature-dependent differences in 2% and 10% BSM macrorheology, whereas in the absence of evaporation control 10% BSM demonstrated a significantly higher viscoelasticity at 37°C. Similarly, during the measurements without evaporation control at 37°C we observed a substantial increase in the storage modulus G′ and the loss modulus G″ of the highly viscoelastic CF sputum but not in the healthy sputum. Conclusion: Our data show systematically higher viscoelasticity of CF compared to healthy sputum at 25°C and 37°C. For measurements at the higher temperature using a solvent trap to prevent evaporation is essential for macrorheological analysis of mucus model systems and native human sputum. Another interesting finding is that the viscoelastic properties are not much sensitive to the applied experimental deformation and yield robust results despite their delicate consistency. The optimized protocol resulting from this work will facilitate standardized quantitative assessment of abnormalities in viscoelastic properties of airway mucus and response to muco-active therapies in patients with CF and other muco-obstructive lung diseases

Complete removal of the lesion as a guidance in the management of patients with breast ductal carcinoma in Situ

Background: Considering highly selected patients with ductal carcinoma in situ (DCIS), active surveillance is a valid alternative to surgery. Our study aimed to show the reliability of post-biopsy complete lesion removal, documented by mammogram, as additional criterion to select these patients. Methods: A total of 2173 vacuum‐assisted breast biopsies (VABBs) documented as DCIS were reviewed. Surgery was performed in all cases. We retrospectively collected the reports of post‐ VABB complete lesion removal and the histological results of the biopsy and surgery. We calculated the rate of upgrade of DCIS identified on VABB upon excision for patients with post‐biopsy complete lesion removal and for those showing residual lesion. Results: We observed 2173 cases of DCIS: 408 classified as low‐grade, 1262 as intermediate‐grade, and 503 as high‐grade. The overall upgrading rate to invasive carcinoma was 15.2% (330/2173). The upgrade rate was 8.2% in patients showing mammographically documented complete removal of the lesion and 19% in patients without complete removal. Conclusion: The absence of mammographically documented residual lesion following VABB was found to be associated with a lower upgrading rate of DCIS to invasive carcinoma on surgical excision and should be considered when deciding the proper management DCIS diagnosis

Draft genome sequences of six listeria monocytogenes strains isolated from dairy products from a processing plant in Southern Italy

Here we announce the draft genome sequences of 6 Listeria monocytogenes strains from ricotta cheese produced in a dairy processing plant located in southern Italy and potentially involved in a multistate outbreak of listeriosis in the United States

Aspectos productivos y educativos de realidades mixtas

En las últimas décadas, y particularmente en los últimos años, hemos asistido a un notable desarrollo de la electrónica que ha propiciado no sólo un crecimiento casi exponencial en la potencia de cómputo, de almacenamiento y de comunicación, sino que además ha permitido crear un gran número de dispositivos de hardware que posibilitan interacciones mucho más naturales y ricas entre los humanos y la tecnología en sí misma. De la misma forma, ha impulsado la creación de más y mejores aplicaciones de Realidad Mixta (RM), concepto relacionado a lo continuo de la virtualidad, donde en un extremo tenemos el mundo físico y real y, en el otro, a la realidad virtual. La realidad mixta es la tecnología que se encuentra entre los dos extremos del continuo de la virtualidad. Dentro de éste podemos encontrar a la Realidad Aumentada (RA), que consiste en adicionar información y objetos del mundo virtual al mundo real para complementarlo. Esta línea de investigación, desarrollo e innovación comprenderá el estudio de los diferentes aspectos de las realidades mixtas con el propósito de diseñar e implementar nuevas aplicaciones enfocadas en aspectos educativos y productivos de la región de influencia de esta Universidad.Eje: Innovación en Sistemas de SoftwareRed de Universidades con Carreras en Informática (RedUNCI

Aspectos productivos y educativos de realidades mixtas

En las últimas décadas, y particularmente en los últimos años, hemos asistido a un notable desarrollo de la electrónica que ha propiciado no sólo un crecimiento casi exponencial en la potencia de cómputo, de almacenamiento y de comunicación, sino que además ha permitido crear un gran número de dispositivos de hardware que posibilitan interacciones mucho más naturales y ricas entre los humanos y la tecnología en sí misma. De la misma forma, ha impulsado la creación de más y mejores aplicaciones de Realidad Mixta (RM), concepto relacionado a lo continuo de la virtualidad, donde en un extremo tenemos el mundo físico y real y, en el otro, a la realidad virtual. La realidad mixta es la tecnología que se encuentra entre los dos extremos del continuo de la virtualidad. Dentro de éste podemos encontrar a la Realidad Aumentada (RA), que consiste en adicionar información y objetos del mundo virtual al mundo real para complementarlo. Esta línea de investigación, desarrollo e innovación comprenderá el estudio de los diferentes aspectos de las realidades mixtas con el propósito de diseñar e implementar nuevas aplicaciones enfocadas en aspectos educativos y productivos de la región de influencia de esta Universidad.Eje: Innovación en Sistemas de SoftwareRed de Universidades con Carreras en Informática (RedUNCI

Il contratto di catering

Il contributo, nell’analizzare le principali caratteristiche del contratto di catering con riferimento alle obbligazioni delle parti ed alle regole che vi presiedono, mira specificamente a rivisitare la sua qualificazione giuridica, valorizzandone la natura di somministrazione di servizi ed escludendone la configurazione quale contratto di appalto o altra figura atipica. Con tale rinnovata prospettiva si è proceduto all’indagine intorno alle norme relative agli elementi costitutivi, all’esecuzione, nonché all’estinzione del contratto e del rapporto