Subchronic safety evaluation of hot-water extract from thinned immature mangos (Mangifera indica ‘Irwin’): 90-days oral toxicity study in rats

application/pdfThinned immature fruit of the mango tree （Mangifera indica ‘Irwin’） are handled as waste. In this study, we conducted a 90-days toxicity study in male and female Sprague Dawley rats to evaluate the safety of a hot-water extract of thinned immature mango fruits （TIMEx） administered by oral gavage at doses of 500, 1000 and 2500 mg/kg body weight/day. Treatment did not result in death or changes in the behavior or external appearance of the animals. No alterations were observed in hematological or serum chemical parameters, urinalysis, food consumption, body weight gain or organ weights at the end of the treatment period, with the exception of higher mean corpuscular volume in male rats that received high doses and lower serum creatine phosphokinase levels in female rats that received medium doses. Under the conditions of this study and based on the toxicological endpoints evaluated, the no-observed-adverse-effect level （NOAEL） for TIMEx was 2500 mg/kg/day. The findings indicate that TIMEx is safe for consumption and should be investigated as a candidate food

２段連続バイオリアクターを用いたタマネギ食酢製造の最適操作

application/pdfOnion vinegar was produced using a 2-stage continuously stirred tank reactor. Regarding the alcohol fermentation and the acetic acid fermentation examined in this study, the immobilized cells on porous ceramics offered stable production of alcohol and acetic acid for long periods of 300 and 700 days, respectively. Compared with the steady-state operation method, the temperature-change forced-cyclic operation method increased ethanol yield of alcohol fermentation by a maximum of 15％. Acetic acid yield in the vinegar showed no difference between the steady-state operation and temperature-change forced-cyclic operation. The onion vinegar produced in this study contained 3.5-11.5 times the amount of organic acids and 1.6-6.9 times the amount of amino acids as commercially sold vinegars.departmental bulletin pape

Fundamental Studies on the Rheological Properties of Fresh Concrete : The Propagation Characteristics of the Elastic Wave in Mortar

departmental bulletin pape

Study of CP-Violating Asymmetries in B0→π+π- Decays

journal articl

昆虫の変態・休眠の分子機構

平成8年度から平成11年度までの4年間に実施された特定領域研究(A)(1)「昆虫の変態・休眠の分子機構(略称:変態・休眠機構)」は,昆虫の有する旺盛な生存,生活戦略を変態と休眠という高次の生命現象に焦点を合わせ,この分子機構を多面的に解明することを目的として,延べ62名の研究者の参加を得て進められ,多くの研究成果を挙げてきた.「変態・休眠を支配するホルモン分子の動態と環境応答」に関する研究では,脳神経ペプチドホルモンの遺伝子の同定,合成分泌とその調節機構,ホルモンの血中動態及びホルモン受容体の解析を進め,新たな知見を得た.また「変態における自己・非自己の認識転換と形作りの分子機構」に関する研究では,幼虫組織の識別と排除機構,成虫組織の分化発生機構,ミツバチの社会性分化をもたらす遺伝子の同定とその発現機構を解明した.これらの成果の一端は平成12年12月神戸市にいて開催された平成12年度文部省主催の「大学と科学」公開シンポジウム「昆虫から学ぶ生きる知恵」において,研究者のみならず一般市民約500名に対し,公表された.一方,総括班員を中心とした研究成果の最終まとめに関する検討をもとにして,学術審議会科学研究費分科会審査第一部会生物系小委員会による特定領域研究研究終了ヒアリングを受け,その結果,Aの評価を得た.また,本研究の成果は,研究目的や研究経過に加え,本研究参加者の研究結果と発表論文リストを編集した研究成果報告書として発刊し,関連機関や研究者等に配布した.科学研究費補助金 研究種目:特定領域研究(A)(1) 課題番号:08276103 研究代表者:山下 興亜 研究期間:1996-2000年度research repor

〈英文〉岐路に立つカナダ他文化主義放送法： 参加型民主主義と文化的多様性の混同

departmental bulletin pape

コウオン カンキョウカ デノ ブンレツ コウボ ノ セイイク セイギョ ニ カカワル Nrp1 オヨビ SPBC17D1.05 ノ ソウゴ サヨウ カイセキ

奈良先端科学技術大学院大学修士(バイオサイエンス)master thesi

mRNA levels of NOS3 in HUVECs in neutrophil-containing culture.

(A) Expression of NOS3/YWHAZ mRNA by RT-PCR using HUVECs alone as a relative control (ratio = 1). (B) H2O2 concentration with and without A23187 stimulation in the supernatants of HUVECs co-cultured with X-CGD neutrophils for 30 minutes compared with healthy control neutrophils. (C)Total nitrate and nitrite concentration in the supernatant of co-cultures of HUVECs incubated with healthy control and X-CGD neutrophils for 30 minutes with and without A23187 stimulation. *P<0.05 compared with the medium and normal neutrophils +A23187 group.</p

Clinical characteristics of X-CGD patients and healthy participants (controls).

Clinical characteristics of X-CGD patients and healthy participants (controls).</p

mRNA levels of <i>EDN1 and</i> ET-1 protein levels in HUVECs in neutrophil-containing culture.

(A) Expression of EDN1/YWHAZ mRNA in HUVECs was examined by RT-PCR and real-time PCR using HUVECs alone as a relative control (ratio = 1). (B) ET1 protein expression and GAPDH in HUVECs in neutrophil-containing transwell system cultures. Representative blots are shown. Arrow at approximately 25kDa indicates ET-1. (C) Quantification of ET-1 bands compared with GAPDH expression. *P<0.05 compared with the HUVECs with normal neutrophils +A23187 group.</p