卓上加工機による複雑形状加工物の加工[ポスター]

開催日時 : 平成17年6月9日、10日 場所 : 理化学研究所　和光研究所　鈴木梅太郎ホールおよび2階ホール　 主催 : 独立行政法人理化学研究所（ＶＣＡＤシステム研究プログラム）textapplication/pdfconference objec

Specialized Pro-Resolving Mediators Do Not Inhibit the Synthesis of Inflammatory Mediators Induced by Tumor Necrosis Factor-α in Synovial Fibroblasts

Background : Tumor necrosis factor (TNF)-α, a proinflammatory cytokine, is involved in the pathogenesis of rheumatoid arthritis (RA). The omega-3 unsaturated fatty acid-derived metabolites resolvin (Rv) D1, RvE1, and maresin-1 (MaR1) have been reported as anti-inflammatory lipid mediators and are known as specialized pro-resolving mediators (SPMs). In this study, we aimed to investigate the anti-inflammatory effects of SPMs on TNF-α-induced responses in synovial fibroblasts. Methods: We investigated the effects of SPMs on gene expression and/or production of cyclooxygenase-2 (COX-2), microsomal prostaglandin E synthase-1 (mPGES-1), interleukin (IL)-6, and matrix metalloproteinase (MMP)-3, which are involved in TNF-α-induced synovitis in RA or OA synovial fibroblasts, by quantitative real-time PCR. We also investigated the effects of SPMs on the mitogen-activated protein kinase (MAPK) signaling pathway by western blotting. Anti-inflammatory effects of SPMs were evaluated by applying SPMs to cultured synovial fibroblasts, followed by TNF-α stimulation. Results: The induction of COX-2, mPGES-1, IL-6, and MMP-3 by TNF-α in synovial fibroblasts was not suppressed by omega 3-derived SPMs regardless of their origin such as RA or OA. SPMs had no effect on lipid mediator receptor gene expression induce by TNF-α and did not inhibit the TNF-α-activated MAPK signaling pathway. The production of COX-2 and IL-6 protein was significantly decreased by p38 inhibitor. Conclusion: Despite reports on the anti-inflammatory effect of omega 3-derived SPMs, its anti-inflammatory effect on TNF-α-induced responses was not observed in synovial fibroblasts. The reason may be that SPMs have no suppressive effect on p38 activation, which plays an important role in the production of inflammatory cytokines in synovial fibroblasts.journal articl

さくら市における地域運営の方向性に関する一考察 ――河戸地区を事例に――

text紀要論文 / Departmental Bulletin Paperdepartmental bulletin pape

Measurement of the B0-B0 mixing rate with B0(B0)→D*∓π± partial reconstruction

journal articl

2007ネンド トショカン ゲンバ エンシュウ ホウコク アサクラシ チュウオウ トショカン イズモ シリツ タイシャ トショカン イバラキシ チュウオウ トショカン ウジシ チュウオウ トショカン オオサカ シリツ チュウオウ トショカン オオサカ フリツ チュウオウ トショカン オオサカ フリツ ナカノシマ トショカン オオタケ シリツ トショカン シガ ケンリツ トショカン センリ コクサイ ガクエン トショカン ドウシシャ ジョシ ダイガク トショ ジョウホウ センター ドウシシャ ジョシ チュウガッコウ コウトウ ガッコウ トショカン ドウシシャ ダイガク ソウゴウ ジョウホウ センター トヤマ シリツ トショカン ナラ ケンリツ トショ ジョウホウカン ヒラカタ シリツ チュウオウ トショカン フクオカ ケンリツ トショカン ヤス トショカン ヤワタ シリツ ヤワタ シミン トショカン リットウ シリツ トショカン

application/pdfdepartmental bulletin pape

付箋紙を用いたフォーカシングの空間づくり（２）

2002-03-25In this paper Clearing a Space of Focusing by posting labels was examined by 4 cases. Focusers were 12 persons. Two focusers did this method of Clearing a Space with a guide individually. Three focusers did their work in group situation. Nine focusers did their work in group situation. A focuser had a concern or issue come up one by one. And she wrote the headline of the concern on the front of a label and the feelings about the concern on the opposite side of it. Then she posted the label somewhere on the white mount. She could choose one among three colors of labels. They were blue, yellow and pink. Focusers repeated this work of putting each concern out of them until the concern came to disappear in their mind. Then they had group review and group sharing. They had two rounds of these procedures. Focusers estimated experiencing this method of Clearing a Space positive and helpful. The number of concerns that came up in the first round decreased in the second round. Some items in the first round came up also in the second round. Several new items came up in the second round. Most focusers posted the labels in the middle area of the mount.departmental bulletin pape

Porphyrin Intercalation in G4-DNA Quadruplexes by Molecular Dynamics Simulations

We investigated the intercalation of a porphyrin derivative (TMPyP) between guanine tetrads (G4-tetrads, G4t’s) in 4-stranded G4-DNA oligomers by classical molecular dynamics simulations. Contrary to experimental evidence on very short oligomers that contain stabilizing cations, we find that TMPyP can stack with the G4-tetrads in the absence of interplane cations. A high TMPyP/G4t stoichiometric ratio of 1/2 induces strong deformations of the G4-quadruplexes. A lower ratio of 1/8 is better compatible with the helical conformation. When a TMPyP is accommodated between two tetrads, the stacking distance between the intercalated molecule and a G4-tetrad is ∼4.3−4.7 Å. We find that the possibility of regular TMPyP intercalation depends on the length of the quadruplex, on the stoichiometric ratio and on the edge termination motif

Ab Initio Optical Absorption Spectra of Size-Expanded xDNA Base Assemblies

We present the results of time-dependent density functional theory calculations of the optical absorption spectra of synthetic nucleobases and of their hydrogen-bonded and stacked base pairs. We focus on size-expanded analogues of the natural nucleobases obtained through the insertion of a benzene ring bonded to the planar heterocycles (x-bases), according to the protocol designed and realized by the group of Eric Kool (e.g., see:  Gao, J.; Liu, H.; Kool, E.T. Angew. Chem., Int. Ed. 2005, 44, 3118, and references therein). We find that the modifications of the frontier electron orbitals with respect to natural bases, which are induced by the presence of the aromatic ring, also affect the optical response. In particular, the absorption onset is pinned by the benzene component of the HOMO of each x-base (xA, xG, xT, xC). In addition, the main trait of the H-bonding interbase coupling is a conspicuous red shift of spectral peaks in the low-energy range. Finally, the hypochromicity, a well-known fingerprint of stacking, is more pronounced in stacked xG−C and xA−T pairs than that in stacked G−C and A−T pairs, an index of enhanced stacking

Stability and Migration of Metal Ions in G4-Wires by Molecular Dynamics Simulations

We present a molecular dynamics investigation of guanine quadruple helices based on classical force fields. We analyze the dependence of the helical conformation on various compositional factors, such as the length of the G4-wire, as well as the incorporation into the helix channel of alkali ions of different species and in different amounts. In compliance with previous indications, our results suggest that monovalent alkali cations assist the stability of the quadruplex arrangement against disruption on the few nanoseconds time scale in the order of increasing van der Waals radius. Whereas very short G4-wire fragments immediately unfold in the absence of coordinating metal ions or in the presence of tiny ions (e.g., Li+) in agreement with the experimental evidence that empty short guanine quadruplexes are not formed in any synthetic conditions, our simulations show that longer empty helices do not discompose. This finding supports the possibility of producing long G4-wires with different guanine-cation stoichiometries than those routinely known. The classical trajectories allow us to identify different stationary axial sites for the different metal species, which are confirmed by complementary quantum calculations

Strain-Dependence of the Electronic Properties in Periodic Quadruple Helical G4-Wires

The electronic structure of periodic quadruple helix guanine wires, which mimic G4-DNA molecules, was studied as a function of the stacking distance between consecutive planes, by means of first principles density functional theory calculations. We show that, whereas for the native DNA interplane separation of 3.4 Å the HOMO- and LUMO-derived bands are poorly dispersive, the bandwidths can be significantly increased when compressive strain is applied along the helical axis. Our findings indicate that efficient band conduction for both holes and electrons can be supported by such wires for stacking distances below 2.6 Å, which imply a huge axial deformation with respect to double and quadruple helices in solutions and in crystals