<Original Article> Sister Chromatid Exchange (SCE) Frequency in Human and Simian Lymphoid Cell Lines Infected with Simian Retroviruses Closely Related to Human T-cell Leukemia Virus Type-1

Sister chromatid exchange (SCE) frequencies were examined on 4 simian lymphoid cell lines producing simian retroviruses closely related to human T-cellleukemia virus type-1 and 6 human lymphoid cells immortalized by these simian viruses. The frequencies of SCE in both simian and human lymphoid cell lines were higher than those in normal cultured simian and/or human lymphocytes. We also observed the change of SCE frequencies in human cell lines immortalized by STLV at several passage levels. At an early passage level the frequency of SCE in these human cell lines was already higher than that in normal lymphocytes culture. This rise in SCE frequency did not change significantly during the observation period for 180 days. From these findings we concluded that human lymphocytes were infected with STLV as simian lymphocytes, and that STLV caused same DNA damage in human lymphocytes as in simian lymphocytes. These phenomena induced by the retrovirus infections may correlate with leukemogenesis .journal articl

Study of double charmonium production in e+e- annihilation at sqrt[s]≈10.6 GeV

journal articl

カンケイ データベース オ モチイタ XML ブンショ ノ カクノウ ト ケンサク シュホウ XRel ノ ジッソウ ト ソノ ヒョウカ

奈良先端科学技術大学院大学修士(工学)master thesi

Unveiling the principle of microRNA-mediated redundancy in cellular pathway regulation

<p>Understanding the multifaceted nature of microRNA (miRNA) function in mammalian cells is still a challenge. Commonly accepted principles of cooperativity and multiplicity of miRNA function imply that individual mRNAs can be targeted by several miRNAs whereas a single miRNA may concomitantly regulate a subset of different genes. However, there is a paucity of information whether multiple miRNAs regulate critical cellular events and thereby acting redundantly. To gain insight into this notion, we conducted an unbiased high-content miRNA screen by individually introducing 1139 miRNA mimics into Chinese hamster ovary (CHO) cells. We discovered that 66% of all miRNAs significantly impacted on proliferation, protein expression, apoptosis and necrosis. In summary, we provide evidence for a substantial degree of redundancy among miRNAs to maintain cellular homeostasis.</p

ЛΕКЦИЯ ПО ИСТОРИИ РУССКОЙ СЛОВΕСНОСТИ, ЧИТАННАЯА. КОЛЕНКО в 1879 г. В РУССКОМ ОТЛЕНИИ ТОКИЙСКОЙШКОЛЫ ИНОСТРАННЫХ ЯЗЫКОВ : ТОКИО ГАЙКОКУГО ГАККО.

研究ノート(Note)application/pdfdepartmental bulletin pape

Supplementary Figure Legends from Dihydroartemisinin Induces Apoptosis by a Bak-Dependent Intrinsic Pathway

Supplementary Figure Legends from Dihydroartemisinin Induces Apoptosis by a Bak-Dependent Intrinsic Pathwa

Transfected SGBS preadipocytes retain their ability for adipogenic differentiation.

<p>Untreated cells, cells treated with transfection agent alone (mock) and cells transfected with a nontargeting (NT) control siRNA were exposed to differentiation medium 48 h after transfection and analyzed on day 14 of adipogenic differentiation. Phase contrast micrographs (A) taken with a 20x objective. Scale bars are 100 µm. Pictures from one representative experiment out of two performed are shown. Differentiation rates (B) and the qPCR analysis of the expression level of an adipocyte marker gene PPARγ (C). The experiment was carried out three times independently and the results are expressed as mean and SEM. ***p<0.0001 untreated vs. SFA.</p

Β. Одоевский. 《Русскне ночи》 (1)

翻訳(Translation)application/pdfdepartmental bulletin pape

Supplementary Figure 1 from Dihydroartemisinin Induces Apoptosis by a Bak-Dependent Intrinsic Pathway

Supplementary Figure 1 from Dihydroartemisinin Induces Apoptosis by a Bak-Dependent Intrinsic Pathwa

B. Oдоевский. 《Русские ночи》 (3)

翻訳(Translation)application/pdfdepartmental bulletin pape