Characterization of marine diatom-infecting virus promoters in the model diatom Phaeodactylum tricornutum

Viruses are considered key players in phytoplankton population control in oceans. However, mechanisms that control viral gene expression in prominent microalgae such as diatoms remain largely unknown. In this study, potential promoter regions isolated from several marine diatom-infecting viruses (DIVs) were linked to the egfp reporter gene and transformed into the Pennales diatom Phaeodactylum tricornutum. We analysed their activity in cells grown under different conditions. Compared to diatom endogenous promoters, novel DIV promoter (ClP1) mediated a significantly higher degree of reporter transcription and translation. Stable expression levels were observed in transformants grown under both light and dark conditions, and high levels of expression were reported in cells in the stationary phase compared to the exponential phase of growth. Conserved motifs in the sequence of DIV promoters were also found. These results allow the identification of novel regulatory regions that drive DIV gene expression and further examinations of the mechanisms that control virus-mediated bloom control in diatoms. Moreover, the identified ClP1 promoter can serve as a novel tool for metabolic engineering of diatoms. This is the first report describing a promoter of DIVs that may be of use in basic and applied diatom research

Remodeling of the Vertebral Body in Hereditary Lordoscoliotic Rabbits Revealed by In Situ Hybridization

The etiology of human idiopathic scoliosis remains still unknown, although several etiologic factors and many animal models of scoliosis have been reported. Kin previously reported radiological and histological findings of spinal deformities in the Hereditary Lordoscoliotic Rabbit (LSR), which develops thoracic lordosis or lordoscoliosis during growth. We investigated biological activities of bone cells of the LSR vertebrae by in situ hybridization. Digoxigenin labeled in situ hybridization probes of mRNA of osteopontin, type I collagen and bone morphogenetic protein (BMP)-2 were prepared. Hybridization was carried out on the sagittal section of lordotic or lordoscoliotic thoracic vertebra in the LSR. In the apical vertebral body and spinous process of the lordotic thoracic spine of LSR during developmental age, stronger positive signals for type I collagen and BMP-2 mRNA were detected in osteoblasts in the endosteum of the ventral portion than those in the dorsal portion of the vertebra, which indicates osteogenesis. In the apical vertebral body of the lordotic thoracic spine, positive signals for osteopontin mRNA were detected in osteoclasts of the Howship’s lacunae and osteocytes around these pits in dorsal endosteal portion, which indicates bone resorption. No such signals were detected in the ventral endosteal portion of the same vertebral body. The vertebral bones of the LSR showed a specific mode of remodeling response to the force producing lordosis.departmental bulletin pape

Incidence rate of needlestick and sharps injuries in 67 Japanese hospitals: a national surveillance study

平成25年

動的問題における数値計算法の精度について

applicationdepartmental bulletin pape

義務教育諸学校の学校事務職員の職務の明確化・人事・人材育成に関する調査報告書

目次 本プロジェクトの目指すもの … 1 目次 ……………………………… 2 研究組織 ………………………… 3 調査成果の概要 ………………… 5 １．本調査の概要 ……………… 11 （１）本調査の目的 ………… 11 （２）調査日程・調査対象者の概要 ………………………… 11 ２．学校事務職員の職務の再定義に取り組んでいる教育委員会の人事・人材育成の特徴 … 14 （１）学校事務職員の職務の再定義の実施状況 …………… 14 （２）学校事務職員の人事に関する取組 …………………… 14 （３）学校事務職員の人材育成に関する取組 ……………… 18 （４）教育委員会が特に重視する資質・能力 ……………… 19 （５）教育委員会が今後特に重視する資質・能力 ………… 22 ３．学校事務職員の再定義の取組内容 ………………………… 26 （１）学校事務職員の職務の再定義の取組内容 …………… 26 （２）学校事務職員の職務の再定義における学校事務の共同実施の取組 ………………… 26 （３）学校事務職員の職務の再定義に取り組む契機・時期，促進要因・阻害要因 ……… 27 ４．学校事務職員の具体的な職務・役割・権限の再設定と通知・規則等での明確化の実態 … 30 （１）学校事務職員の具体的な職務・役割・権限の再設定と通知・規則等での明確化 … 30 （２）学校事務職員の具体的な職務・役割・権限の再設定と通知・規則等での明確化の 取組 …………………… 30 ５．学校事務職員の職務の明確化・人事・人材育成の現状認識と今後の展望 ……………… 32 （１）学校事務職員の職務の明確化・人事・人材育成に関する現状認識 ………………… 32 （２）学校事務職員向けの公的研修の課題 ………………… 32 （３）学校事務職員の職務の明確化・人事・人材育成に関する今後の国レベルの取組に 対する期待 …………… 33 自由記述 ………………………… 37 調査票 …………………………… 45research repor

村上左一郎の信仰と民権

departmental bulletin pape

toratoratora

2022 ねこ展 210×297 mm 色鉛筆、アルコールマーカー、Photosho

De filio prodigo, sive Exhortatio peccatoris ad poenitentiam.

Copia digital. Madrid : Ministerio de Cultura. Subdirección General de Coordinación Bibliotecaria, 200