Impact of phenol-enriched virgin olive oils on serum metabonome and its relationship with cardiometabolic parameters

Phenol-rich foods consumption such as virgin olive oil (VOO) has been shown to have beneficial effects on cardiovascular diseases. The broader biochemical impact of VOO and phenolenriched OOs remains, however, unclear. A randomized, double-blind, cross-over, controlled trial was performed with thirty-three hypercholesterolemic individuals who ingested for 3-weeks (25 mL/day): (1) an OO enriched with its own olive oil phenolic compounds (PCs) (500 ppm; FOO); (2) an OO enriched with its own olive oil PCs (250 ppm) plus thyme PCs (250 ppm; FOOT); and (3) a VOO with low phenolic content (80 ppm). Serum lipid and glycemic profiles, serum 1H-NMR spectroscopybased metabolomics, endothelial function, blood pressure, and cardiovascular risk were measured. We combined OPLS-DA with machine learning modelling to identify metabolites discrimination of the treatment groups. Both phenol-enriched OO interventions decreased the levels of glutamine, creatinine, creatine, dimethylamine, and histidine in comparison to VOO one. In addition, FOOT decreased the plasma levels of glycine and DMSO2 compared to VOO, while FOO decreased the circulating alanine concentrations but increased the plasma levels of acetone and 3-HB compared to VOO. Based on these findings, phenol-enriched OOs were shown to result in a favorable shift in the circulating metabolic phenotype, inducing a reduction in metabolites associated with cardiometabolic diseases.We are grateful to the CERCA Program/Generalitat de Catalunya for institutional support. J.M.-P. acknowledges the support of the Instituto de Salud Carlos III (ISCIII) through project PI20/01090 co-funded by the European Union under the European Regional Development Fund (FEDER) ‘A way to make Europe’ and project CP18/00009 co-funded by the European Union under the European Social Fund (FSE) ‘Investing in your Future’

A comparative evaluation of the phenol and lycopene content of tomato by-products subjected to different drying methods

This study is focused on exploring tomato by-products to provide ingredients rich in bioactive compounds with higher functional value through applying innovative techniques (microwave and spiral flash dryer) and conventional techniques (freeze-drying and hot air-drying) to the dehydration of industrial tomato pomace. The effect of the drying method on the bioactive composition of the tomato by-products (phenols, lycopene and soluble dietary fiber) was analyzed. The phenolic composition was determined using a targeted chromatographic approach based on UHPLC-QqQ-MS/MS. Large amounts of naringenin (194.7¿949.4 mg/kg) were detected, together with quercetin, caffeic acid, coumaric acid and 4-hydroxybenzoic acid. Microwave dehydration improved the retention of flavanone-like compounds, especially naringenin, and lycopene, while tomato products dehydrated with Spiral Flash dryer showed higher concentrations of flavonols and phenolic acids. The results showed that the industrial application of drying processes using Spiral Flash, and especially with microwaves, could be promising for producing high added-value ingredients from tomato by-products.The authors are grateful to the technical staff, Cristina Moreta and Miguel Ángel Fernández-Recio, of the Instrumental Analysis Service at the ICVV by their UHPLC-QqQ-MS/MS analytical and technical support in the phenol analysis. This work has been co-financed by the European FEADER funds, the Ministry of Agriculture and Food of Spain and the Government of La Rioja, through the project funded in call PDR “plusPRODUCT: By-product valorization and development of the Circular Economy in the Agrifood Industry” (reference: 23M/20) and pre-doctoral grants