Transfer of a chloroplast-bound precursor protein into the translocation apparatus is impaired after phospholipase C treatment

We have studied the influence of phospholipase C treatment of intact purified chloroplast on the translocation of a plastid destined precursor protein. Under standard import conditions, i.e. in the light in the presence or 2 mM ATP translocation was completely abolished but binding was observed at slightly elevated levels. An experimental regime which allowed binding but not import of the precursor protein, i.e. in the dark in the presence of 10 μM ATP, demonstrated that translocation intermediates, normally detected at this stage, were missing in phospholipase treated chloroplasts. The precursor was completely sensitive to protease treatment, indicating that the transfer of the precursor from the receptor to the import apparatus was blocked by phospholipase treatment

An Evaluation of The Home Economics News Packet

When it comes to getting Extension news to editors, county home economists are effective relays

Neutrality, Partiality, and Meaning in Life

Discussion of whether values and norms are neutral or not has mainly appeared in works on the nature of prudential rationality and morality. Little systematic has yet appeared in the up and coming field of the meaning of life. What are the respects in which the value of meaningfulness is neutral or, in contrast, partial, relational, or ‘biased’? In this article, I focus strictly on answering this question. First, I aim to identify the salient, and perhaps exhaustive, respects in which issues of neutrality arise in the contexts of life’s meaning. In addition to providing a taxonomy of the key points of contention, a second aim is to advance reflection about them by considering the most important arguments that have been marshalled in favour of one side or the other, particularly as they appear in recent neutral positions. I conclude that meaning in life is neutral with respect to time but not any other conditions such as agents and patients, with a third aim being to point out that this makes the value of meaning different from the kinds of non/neutrality encountered in some salient conceptions of prudence and morality

Utilitarianism and animal cruelty: Further doubts

Utilitarianism has an apparent pedigree when it comes to animal welfare. It supports the view that animal welfare matters just as much as human welfare. And many utilitarians support and oppose various practices in line with more mainstream concern over animal welfare, such as that we should not kill animals for food or other uses, and that we ought not to torture animals for fun. This relationship has come under tension from many directions. The aim of this article is to add further considerations in support of that tension. I suggest three ways in which utilitarianism comes significantly apart from mainstream concerns with animal welfare. First, utilitarianism opposes animal cruelty only when it offers an inefficient ratio of pleasure to pain; while this may be true of eating animal products, it is not obviously true of other abuses. Second, utilitarianism faces a familiar problem of the inefficacy of individual decisions; I consider a common response to this worry, and offer further concerns. Finally, the common utilitarian argument against animal cruelty ignores various pleasures that humans may get from the superior status that a structure supporting exploitation confers

Optimal damping profiles for a heaving buoy wave-energy converter

This paper explores optimal damping profiles for a heaving buoy wave energy converter (WEC). The approach is mathematical and the model of Eidsmoen (1995) is used as a basis. In order to permit analytical development, the model is initially simplified and an optimal damping profile is determined using numerical optimization. Having found the optimal damping profile, a semi-analytical solution methodology is developed to determine the optimal damping parameters. Finally, the procedure is validated on the original model and some aspects related to the control problem are addressed

Meaning in Life and the Metaphysics of Value

According to subjectivist views about a meaningful life, one's life is meaningful in virtue of desire satisfaction or feelings of fulfilment. Standard counterexamples consist of satisfaction found through trivial or immoral tasks. In response to such examples, many philosophers require that the tasks one is devoted to are objectively valuable, or have objectively valuable consequences. I argue that the counterexamples to subjectivism do not require objective value for meaning in life. I also consider other reasons for thinking that meaning in life requires objective value and raise doubts about their strength. Finally, I argue that beauty is not plausibly objective, but that it seems important for meaning. This puts pressure on the objectivist to explain why objectivity matters in the case of other values

On Grasping a Tumbling Debris Object with a Free-Flying Robot

The grasping and stabilization of a tumbling, non-cooperative target satellite by means of a free-flying robot is a challenging control problem, which has been addressed in increasing degree of complexity since 20 years. A novel method for computing robot trajectories for grasping a tumbling target is presented. The problem is solved as a motion planning problem with nonlinear optimization. The resulting solution includes a first maneuver of the Servicer satellite which carries the robot arm, taking account of typical satellite control inputs. An analysis of the characteristics of the motion of a grasping point on a tumbling body is used to motivate this grasping method, which is argued to be useful for grasping targets of larger size

Surveillant assemblages of governance in massively multiplayer online games:a comparative analysis

This paper explores governance in Massively Multiplayer Online Games (MMOGs), one sub-sector of the digital games industry. Informed by media governance studies, Surveillance Studies, and game studies, this paper identifies five elements which form part of the system of governance in MMOGs. These elements are: game code and rules; game policies; company community management practices; player participatory practices; and paratexts. Together these governance elements function as a surveillant assemblage, which relies to varying degrees on lateral and hierarchical forms of surveillance, and the assembly of human and nonhuman elements.Using qualitative mixed methods we examine and compare how these elements operate in three commercial MMOGs: Eve Online, World of Warcraft and Tibia. While peer and participatory surveillance elements are important, we identified two major trends in the governance of disruptive behaviours by the game companies in our case studies. Firstly, an increasing reliance on automated forms of dataveillance to control and punish game players, and secondly, increasing recourse to contract law and diminishing user privacy rights. Game players found it difficult to appeal the changing terms and conditions and they turned to creating paratexts outside of the game in an attempt to negotiate the boundaries of the surveillant assemblage. In the wider context of self-regulated governance systems these trends highlight the relevance of consumer rights, privacy, and data protection legislation to online games and the usefulness of bringing game studies and Surveillance Studies into dialogue