EFFECT OF MICROALGAE ON GROWTH AND FATTY ACID PROFILES OF HARPACTICOID COPEPOD, Tisbe holothuriae

Growth of marine copepods is influenced by feed. The purposes of this trial were to observe both growth and fatty acid compositions of harpacticoid copepod nauplii, Tisbe holothuriae by feeding with several microalgal species in laboratory: (A) Isochrysis tahiti; (B) Nannochloropsis oculata; (C) Rhodomonas sp., and (D) Tetraselmis chuii. The trial was carried out for 35 days with randomized complete design and triplicates in each treatment. The results showed that final copepod nauplii densities were not significantly different (P>0.05) in all treatments. However, lipid content of copepod nauplii fed with T. chuii was significantly higher (P<0.05) compared to that of other treatments while fatty acid profiles of EPA, DHA and DHA/EPA ratios showed both insignificant and significant differences among treatments

Pelatihan Program Pengembangan Keprofesian Berkelanjutan (Pkb) Untuk Guru Sekolah Dasar Di Lingkungan Dinas Pendidikan Dan Kebudayaan Kota Sukabumi

Becoming a teachers means always encourage yourself for longlife education. This is one of the benefit in Teacher career. We used to be learn anything and everything to update our professionalism. As a Teacher, we should be aware of teachers world, knowledge, technology, and student needs changing. Indonesian Government facilitate it through PKB (Continuity Profesionalism Development) program. PKB goals is to increasing teachers career competence continualy. Although Government publish this program since 2009, Education and Culture Departement of Sukabumi City started it in 2015 October. This lateness becoming our concern to organize a PKB Workshop specialy for elemetary school teachers. This workshop attendence by fifty teachers from seven distrik in Sukabumi city. The Workshop goals is to diseminate PKB and how to running it in teachers career. Beside that, the workshop also trained teacher how to entablish class action reasearch. This workshop held in three days from 2nd to 4th November 2016. From the workshop we know that PKB is very important to increase teachers competence and to maintain teachers career

PRELIMINARY STUDY ON POPULATION DYNAMIC OF HARPACTICOID COPEPOD Euterpina acutifrons IN CULTURE CONDITION

The most important factor to high mortality rate in larval rearing is feeding success in early larval stage related to kind and size of natural live food. Copepod basically is the main source of natural food in the open ocean having some advantages such as smaller size of nauplii, attractive movement and high nutritional value. Observation on population dynamic of harpacticoid copepod Euterpina acutifrons was carried out using 5-L plastic bucket with initial density 100 ind./L. Green algae Nannochloropsis sp. was added to culture media at density of 50,000 cells/mL as a basic feed and additional feeds given were wheat flour (group A) and chicken liver (group B) at a rate of 50 mg/bucket. The result showed that there was no difference on population pattern in both groups where the incubation time took eight days to hatch, from nauplii to the copepodite stage was three days and from copepodite to adult copepod took five-to-six days. The differences came up from population number: in group (A) the highest number of copepod-bearing-egg was only 133 ind., nauplii production up to 62,833 ind. and number of copepodites was 22,333 ind. lower compared to group (B) with the highest copepod-egg was 308 ind., nauplii was 113,333 ind. and copepodite was 51,167 ind. The conclusion pointed out that the kind of food did not influence population pattern (quality) but gave effect to population growth

Pengaruh Pemberian Nata De Nanno Dan Nannochloropsis Oculata Terhadap Populasi Brachionus Plicatilis [Effect of Giving Nata De Nanno and Nannochloropsis Oculata on Population of Brachionus Plicatilis]

This study aims to determine the effect of Nata De Nanno and Nannochloropsis oculata for Brachionus plicatilis population. The research method used was experimental with a Completely Randomized Design (CRD) with six treatments and four replications. The treatments used were: (A) Nata de Nanno 5x109 cells/L N. oculata, (B) Nata de Nanno 4x109 cells/L N. oculata, (C) Nata de Nanno 3x109 cells/L N. oculata, (D) N. oculata 5x109 cells/L, (E) N. oculata 4x109 cells/L and (F) N. oculata 3x109 cells/L. Analysis of data using variant analysis (ANOVA) and to determine the differences between treatments performed tests Distance Regression Duncan. The results showed that administration of Nata De Nanno and Nannochloropsis oculata for Brachionus plicatilis populations exert a significantly different (p<0,05) in each treatment delivery Nata De Nanno and Nannochloropsis oculata with the highest peak population of young B. plicatilis achieved on the fifth day of treatment D (N. oculata) with a concentration of 5x109 cells/L produce a population young B. plicatilis as much as 98,5 individuals/ml. The lowest peak population of young B. plicatilis on the fifth in treatment C (Nata de Nanno) with a concentration of 3x109 cells/L N. oculata 76,75 produce a population of young B. plicatilis 76,75 individuals/ml. Water quality parameters during the study was the temperature ranges between 27-31°C, a salinity of 31 ppt, pH value 7, DO (Dissolved oxygen) ranged between 5-8 mg/L, and ammonia levels ranged from 0-0.25 mg/L

FATTY ACID PROFILES OF CYCLOPOID COPEPOD NAUPLII Apocyclops panamensis AND THE EFFECTS OF SALINITY CHANGE

Lipid and fatty acid profiles were described for copepod nauplii Apocy clops panamensis from fertilized brackish water ponds, and after being acclimated to fullsea water salinity. Mean total lipid content of copepod nauplii collected from ponds fertilized with inorganic fertilizer combined either with alfalfa meal, rice bran, wheat bran, and a combination of these fertilizers ranged from 5.66 ± 0.15 to 7.76% ± 0.27%. Non-polar (neutral) lipid fraction of pond copepod nauplii was a significantly higher percentage of the total lipid content (74.5 ± 1.8 - 93.5% ± 1.0%) compared to those of polar lipid (6.5 ± 1.0 - 21.3% ± 1.8%) (P= 0.000). DHA/EPA ratio in neutral lipids ranged from 1.8 ± 0.2 - 2.0 ± 0.1 with no significant differences in three fertilization regimes. DHA was 27.5% ± 0.56% of the neutral lipids and EPA 14.8% ± 0.8%. Acclimation of copepod nauplii for six hours from brackish to full-sea water salinity reduced their lipid content and individual dry weight significantly. Mean total lipid content was reduced 44.2%, non-polar lipid was reduced 46.9% and polar lipid was reduced 24.4%.Acclimation altered the DHA/EPA ratio, in the neutral fraction the ratio increased 26.3% but in the polar fraction it decreased 25%

RESOLUTION OF A RACEMIC ISOLEUCINE MIXTURE BY USING METHYL L-MANDELATE

A methyl ester of L-mandelic acid was found to be an effective resolving agent for resolution of commercial DL-isoleucine. The resolution was based on Steglich esterification between methyl L-mandelate and Boc-DL-isoleucine. The two resolved isomers were easily separated by using a conventional flash-column chromatography, giving quantitatively good yields. Unfortunately, the methyl L-mandelate was found to be ineffective to resolve four stereoisomers of Fmoc- isoleucine

Optimalization of Feed Dosage and Frequency in Production of Rotifer (Brachionus Rotundiformis)

The availability of rotifer as live food is importance in a larval mariculture. Therefore a continuous and high production of rotifer is needed. The study was aimed to determine the frequency and dosage of the best food in rotifer culture. Nannochloropsis oculata, yeast and scott\u27s emulsion are used as potential feeds for rotifer. Three dosages of N. oculata (150,000; 250,000, and 350,000 cells/ind.rotifer/day) were applied in the study and were given twice per day. Meanwhile, yeast(0.5 g/106 ind./day) and Scott\u27s emulsion with different dosages (2, 4, and 8 μg/106 ind./day) were given with two different feeding frequencies (two and four times a day) for each dosages. Each treatment was done in triplicates. Sampling of rotifer was conducted in the morning (AM) and afternoon (PM). Water quality (temperature, dissolved oxygen and ammonia) was also measured. Growth and productivity of rotifer were determined from the number of rotifer and the number of rotifer eggs, respectively. The results showed that the optimum productivity of rotifer was achieved by giving N. occulata of 250,000 sel/ind/day, twice a day in four days culture. Meanwhile, treatment with yeast and Scott\u27s emulsion gave best performance when applying 0.5 g/106 and 2 μg/106 ind./day twice per day, respectively. Yeast and scott\u27s emulsion treatment yielded optimum production in two days of culture

KULTUR ROTIFER DENGAN BEBERAPA JENIS PAKAN DAN KOMBINASINYA

Tujuan penelitian untuk mengetahui jenis dan kombinasi pakan yang terbaik pada kultur rotifer secara intensif. Penelitian menggunakan pakan alga Nannochloropsis oculata, ragi roti (Saccharomyces cerevisiae), vitamin B dan Scott’s emulsion, perlakuan pakan A) N.oculata, B) ragi roti + Scott’s emulsion, C) Ragi roti + Scott’s emulsion + Vit.B12, dan D) N.oculata + ragi roti + Vit.B12 + Scott’s emulsion. Wadah penelitian menggunakan kontainer plastik putih volume 20 L. dengan media pemeliharaan 18 L, densitas awal adalah 56 ekor rotifer/mL. Produksi tertinggi adalah 33,4 juta ekor         rotifer (1.867 ekor rotifer/mL) pada perlakuan D) dengan persentase produksi telur harian mencapai 29%. Peringkat kedua produksi ada pada perlakuan A) yang hanya mengandalkan N. oculata. Hasil pengamatan menunjukkan peranan vital alga sebagai dasar sumber energi yang akan berlipat hasilnya dengan penambahan suplemen yang essensial untuk memacu kemampuan reproduksi rotifer. Intensifikasi budidaya dan produksi rotifer dapat dikembangkan dengan pakan dasar alga dikombinasikan dengan suplemen essensial, dan didukung pengaturan kondisi pencahayaan yang tepat, diyakini akan memberikan hasil yang optimal.The aim of this study was to find out types of feed and their combinations for intensive culture of rotifer. Feeds used were Nannochloropsis oculata, beaker yeast (Saccharomyces cerevisiae), vitamin B12 and Scott’s emulsion. The treatments consisted of A) N.oculata, B) beaker yeast + Scott’s emulsion, C) beaker yeast + Scott’s emulsion + Vit.B12, and D) N. oculata + beaker yeast + Vit.B12 + Scott’s emulsion. White plastic containers were used for culturing the rotifer in 18 L. culture medium, with initial density of 56 ind./mL. The highest production was 33.4 million rotifers (1,867 rotifer/mL) in treatment D with daily production of eggs of 25%, and the second was reached by treatment A) (algae only). This result showed the important role of algae as a basic energy source for growth and rotifer reproduction can be accelerated with the addition of essential supplements. Development of intensive culture of rotifer using algae as a basic feed, combined with essential supplements, and cultured under appropriate light intensity could result in optimum growth of rotifer