Studies on the groups of soil bacteria in mangrove forest with different salt stress and its function

摘要红树林是热带亚热带海岸潮间带的一种常绿阔叶林，盐生生境是该生态系统的重要特征之一；目前，已经有不少关于红树植物如何适应盐生生境的形态解剖和生理生化特征报道，关于盐度对其适应机制的影响的研究也大量出现，但是，国内外盐度对红树林区微生物尤其是细菌的影响研究未见报道。红树林区含有丰富的微生物资源，尤其是底部沉积物，其微生物数量比海水丰富得多，这些微生物长期适应红树林区潮间带的盐生生境，形成了红树林区特有的微生物类型，至今尚无关于盐胁迫下红树林微生物类群的研究用以分离筛选出红树林中的耐盐、嗜盐微生物。本试验以九龙江口秋茄林为研究对象，利用不同培养基从林内表层土壤微生物筛选出各种嗜盐细菌，找出其最适...Abstract Mangrove is one of evergreen broad-leaved forests in tropical sub-tropical tideland.Salt environment is one of important characteres for this kind of ecosystem. At present, there are some research on the morphology-anatomy and physiological biochemistry about how mangrove is adapted to the salt environment. At the same time,many reseaches on how the salt concentration affects the adap...学位：理学硕士院系专业：生命科学学院生物学系_植物学学号：20022600

Microorganism resource of mangrove ecosystems

随着工业的迅猛发展,工业废料源源不断地向海洋输出,污染日趋严重。人们在大力开发海洋微生物自净能力的同时,也对海岸线的绿色卫士———红树林给予了密切关注,积极展开红树林区微生物资源的开发利用。本文从红树林区微生物库的资源多样性、微生物在物质循环和能量流动中的作用、生理活性物质、代谢产物和污染治理等几个方面进行综述。 【英文摘要】 With the rapid development of industry,more and more industrial waste is constantly exported to the ocean which causes serious pollution problems.Researchers are paying more attention to the mangrove,the important guard of the coastline,and the exploitation of mangrove microorganism resource has been expanded in many aspects,as well as developing the self-clean ability of ocean microorganism.This review summarized the diversity of mangrove microorganism resource,the role of microorganism in material recycle...国家自然科学基金资助项目(30270272)

不同群落类型的长苞铁杉林的根际微生物研究

对长苞铁杉纯林、长苞铁杉猴头杜鹃混交林和长苞铁杉毛竹混交林3个不同群落类型的根际微生物进行研究,发现3个不同群落的微生物数量及组成各不相同,其具体大小关系为(单位:cfu/g·(drymass)×104):长苞铁杉纯林:根际细菌(20.00)>非根际细菌(12.00)>根际真菌(9.37)>非根际真菌(6.74)>根际放线菌(2.69)>非根际放线菌(1.83);长猴混交林:根际细菌(34.90)>非根际细菌(18.00)>根际真菌(6.54)>非根际真菌(3.78)>根际放线菌(2.96)>非根际放线菌(1.76);长毛混交林:非根际细菌(88.00)>根际细菌(68.60)>非根际放线菌(13.40)>根际放线菌(12.60)>根际真菌(4.64)>非根际真菌(3.46).通过比较根际与非根际土壤微生物得出不同群落类型长苞铁杉的根际效应,3个群落类型的总微生物的根际效应大小为:长猴混交林(1.87)>长苞铁杉纯林(1.56)>长毛混交林(0.82)

College laboratory hazardous waste treatment status and countermeasures

随着我国高等教育的快速发展,高校实验室特别是化学、生物类实验室的数量和规模不断地增多和扩大,实验室产生的危险废物越来越多,对环境污染的风险也越来越大。通过对我国普通高校实验室产生危险废物的处置现状进行原因分析,提出了对实验室危险废物处置的对策思考。With the rapid development of China＇s higher education, university laboratories, especially chemistry, biology laboratory the number and size continue to increase and expand, more and more hazardous waste generated by the laboratory, the risk of environmental pollution is also growing big. Cause analysis and countermeasures laboratory hazardous waste disposal by the disposal of hazardous waste generated by the status quo of college laboratory

Isolation and identification of Bacillus cereus producing cellulase and enzymatic properties analysis

【目的】筛选高效分泌纤维素酶的细菌,为纤维素资源利用提供理论依据。【方法】以羧甲基纤维素钠(CMC-Na)为唯一碳源,对通过筛选培养基从森林湿泥样品中分离筛选出的细菌,采用刚果红染色、革兰氏染色和生化特性及16SrDNA进行鉴定,并对菌株产酶性质进行初步研究。【结果】获得一株纤维素酶产生菌L-30,该菌株在pH4.8、50℃条件下的酶活力为4.25 U/mL,经鉴定L-30为蜡样芽孢杆菌。酶学性质研究表明,L-30菌株所产纤维素酶最适反应pH为6.0,最适温度为50℃,该条件下酶活力最高,达4.95 U/mL,该酶对CMC-Na具有较强的分解能力;L-30菌株在最佳生长条件下,于接种后48 h即可达到产酶高峰,L-30菌株的产纤维素酶能力能稳定遗传。【结论】分离到的L-30为一株高产纤维素酶蜡样芽孢杆菌,其酶学性质好,具有进一步开发利用的价值。[Objective]Cellulase, efficiently producing bacteria, was screened to provide references for cellulose application.[Method]Bacteria was screened by using CMC-Na as the only carbon source from humid soil sample. The methods of Congo red staining, gram staining, biochemical character and molecular property of 16S rDNA were used to identify the bacteria, and the enzymatic properties of the cellulose were also observed. [Result]One strain of cellulase producing bacteria （L-30） was isolated from the humid soil sample. The cellulase activity was 4.25 U/mL in the conditions of pH 4.8 and 50℃. L-30 was identified as Bacillus cereus. The preliminary study on enzymatic properties showed that under the optimum conditions of pH of 6.0 and temperature of 50℃, the cellulose activity reached 4.95 U/mL in this reaction system. The cellulase showed high ability to degrade CMC-Na. L-30 reached its largest amount of the enzyme after cultured for 48 hours and displayed genetic stability on secreting enzyme. [Conclusion]The isolated Bacillus cereus of L-30 with good enzymatic properties should be further developed and utilized.广西林产化学与工程协同创新高水平研究项目(2013B01)； 广西自然科学基金项目(2012GXNSFAA053060)； 广西高校科学技术研究项目(2013YB069

Reform and Exploration of Literature Retrieval Course Teaching

文献检索课是高校培养学生信息素养的重要课程。根据药学专业的培养目标和教学特点,从培养学生信息意识,提高药学文献检索技能和信息利用能力为着眼点,针对药学文献检索课程教学过程中出现的问题,在教学内容、教学方法及应用训练等方面进行改革和实践,取得了良好的效果。 Literature Retrieval course was an important course which cultivated students＂ intbrmation literacy. According to the training target and teaching characteristics of pharmacy, the information consciousness of students, the skills of retrieval and the ability of using information were focused on. In the light of the teaching problems from this course, some countermeasures on teaching contents, teaching methods and training were provided, and good teaching effects were obtained

CD44与不同分子量HA相互作用的差异以及对细胞黏附的影响

目的 CD44是一种I型跨膜糖蛋白,广泛分布于肿瘤细胞、免疫细胞、干细胞等,其与配体的相互作用介导细胞基质互作和细胞与细胞黏附。透明质酸HA是CD44的重要配体之一,二者相互作用在介导肝脏免疫反应中具有重要作用,其生物学功能与HA分子量密切相关。已有研究表明,低分子量HA促进炎症,而高分子量HA则抑制炎症。因此,探究CD44与不同分子量HA的相互作用对于理解上述生物学功能具有重要意义

Study on extraction process of total flavoniods and

目的:对宽叶金粟兰中总黄酮的提取工艺及抗氧化活性进行了研究,旨在为其开发利用提供理论依据。方法:采用微波辅助提取法对宽叶金粟兰中总黄酮的提取工艺进行优化,采用分光光度法测定提取物的还原力,并研究其对羟基自由基、DPPH自由基的清除作用。结果:最佳提取条件为:乙醇体积分数60%、料液比1∶30、微波功率300W,微波处理时间15min;在此条件下宽叶金粟兰的总黄酮提取率为6.11%。在实验浓度(0.2~0.8mg/mL)范围内,宽叶金粟兰黄酮对羟基自由基的清除率可达到100%,对DPPH自由基的清除率达到76.2%,同时还具有较强的还原能力。结论:研究结果表明宽叶金粟兰中的黄酮类物质具有较强的抗氧化活性,该植物具有一定的开发和应用前景。Abstract: Objective: To study the extraction technology of total flavonoids from Chloranthus henryi and its antioxidant activity.Methods: The optimum condition for extracting total flavonoids from Chloranthus henryi by microwave － assisted was obtained through orthogonal experiments.And the antioxidant activity of the extract was evaluated by spectrophotometry on its ability of scavenging hydroxyl free radical(·OH) and 1－ diphenyl－2－ picrylhydrazy free radical( DPPH·) ，total reducing power.Ｒesults: The optimum procedure for microwave－ assisted extraction of total flavonoids were ethanol concentration of 60%，ratio of material to solvent of 1 ∶ 30，microwave power of 300W， microwave time of 15min and that the flavonoids yield was up to 6.11%.The scavenging rates of total flavonoids from Chloranthus henryi against hydroxyl free radical and DPPH free radical were found to be 100% and 76.2%，and the extract also had a strong total reducing power at a certain concentration range( 0.2 ～ 0.8mg /mL) . Conclusion: The results showed that the extract from Chloranthus henryi was active for oxidation resistance，and it showed good application future for this plant.广西高校科学技术研究项目(YB2014100