目的:对宽叶金粟兰中总黄酮的提取工艺及抗氧化活性进行了研究,旨在为其开发利用提供理论依据。方法:采用微波辅助提取法对宽叶金粟兰中总黄酮的提取工艺进行优化,采用分光光度法测定提取物的还原力,并研究其对羟基自由基、DPPH自由基的清除作用。结果:最佳提取条件为:乙醇体积分数60%、料液比1∶30、微波功率300W,微波处理时间15min;在此条件下宽叶金粟兰的总黄酮提取率为6.11%。在实验浓度(0.2~0.8mg/mL)范围内,宽叶金粟兰黄酮对羟基自由基的清除率可达到100%,对DPPH自由基的清除率达到76.2%,同时还具有较强的还原能力。结论:研究结果表明宽叶金粟兰中的黄酮类物质具有较强的抗氧化活性,该植物具有一定的开发和应用前景。Abstract: Objective: To study the extraction technology of total flavonoids from Chloranthus henryi and its antioxidant
activity.Methods: The optimum condition for extracting total flavonoids from Chloranthus henryi by microwave －
assisted was obtained through orthogonal experiments.And the antioxidant activity of the extract was evaluated by
spectrophotometry on its ability of scavenging hydroxyl free radical(·OH) and 1－ diphenyl－2－ picrylhydrazy free
radical( DPPH·) ，total reducing power.Ｒesults: The optimum procedure for microwave－ assisted extraction of total
flavonoids were ethanol concentration of 60%，ratio of material to solvent of 1 ∶ 30，microwave power of 300W，
microwave time of 15min and that the flavonoids yield was up to 6.11%.The scavenging rates of total flavonoids from
Chloranthus henryi against hydroxyl free radical and DPPH free radical were found to be 100% and 76.2%，and the
extract also had a strong total reducing power at a certain concentration range( 0.2 ～ 0.8mg /mL) . Conclusion: The
results showed that the extract from Chloranthus henryi was active for oxidation resistance，and it showed good
application future for this plant.广西高校科学技术研究项目(YB2014100
