Characterization and application of nucleocapsid protein of porcine reproductive and respiratory syndrome virus expressed in E.coli

从已构建的PRRSV ORF7重组质粒pUCm-T-ORF7中用PCR扩增ORF7基因亚克隆至表达载体pGEX-4T-3,构建重组表达质粒pGEX-4T-3-ORF7并转化大肠杆菌。经SDS-PAGE及Western blotting鉴定,成功表达了谷胱苷肽转移酶(GST)融合的核衣壳蛋白(N),重组N蛋白表达量约为菌体总蛋白的35%,主要以可溶的形式存在,且能形成同源二聚体。重组N蛋白经谷胱苷肽凝胶(glutathione sepharose 4B)亲和层析后得到高度纯化,并将该蛋白作为抗原建立了间接ELISA检测方法。利用该方法对某猪场76份猪血清进行检测并将结果与IDEXX公司ELISA试剂盒检测结果作比较,2种方法的总符合率达93.4%,检测结果之间差异不显著(P>0.05)。结果表明大肠杆菌表达的重组GST融合N蛋白具有良好的抗原性,因而有望利用该重组蛋白开发为试剂盒应用于临床PRRSV抗体的检测。Porcine reproductive and respiratory syndrome virus(PRRSV) FJ-1 was a newly identified virus isolate in Fujian province.The ORF7 gene of FJ-1 was amplified by RT-PCR and cloned into vector pUCm-T,then subcloned into expression vector of pGEX-4T-3.The recombinant GST-tagged nucleocapsid protein(rN) was expressed in E.coli and the molecular weight was approximately 38 000 as identified by SDS-PAGE and Western blotting.Expression level of the rN protein was approximately 35% of the total bacterial protein and mostly soluble.The rN protein was purified to homogeneity using GST affinity chromatography.Analysis of the rN protein under nonreducing conditions revealed that similar to native protein,the rN protein also possesses homo-dimerization property.An indirect enzyme-linked immunosorbent assay(ELISA) for detecting PRRSV antibody was developed using the purified rN protein as antigen.76 serum samples were detected by the method and the result was compared with that using IDEXX PRRS HerdChek ELISA kit.An identity of 93.4% was revealed between the two ELISA kits and no significant difference(P>0.05) was detected.The data indicate the rN protein has the potential usefulness for detection of the PRRSV antibodies.福建省科技攻关计划重点资助项目(2003N083);; 厦门市科技攻关计划重点资助项目(3502Z20031054

急性脑卒中患者的记忆障碍与肽类物质的关系

本实验对12例缺血性脑血管病(ICVD)患者及12例对照者和12例正常健康对照者进行了临床记忆量表检查,并测定了患者及对照者的CSF、VIP及SS含量。结果:(1) ICVD患者的记忆总分及MQ均显著低于两个对照组;分项测试中联想学习,图像自由回忆成绩亦显著降低。(2) ICVD患者CSF SS含量显著低于对照组。(3) ICVD患者CSF SS含量与MQ呈显著正相关。本结果提示ICVD患者有不同程度的记忆障碍,SS与记忆有关,测定CSF SS对患者记忆状况的判断有参考价值

NMR Studies on Interactions between Diperoxovanadate and Imidazole-like Ligands

为探讨咪唑环上取代基团对反应平衡的影响,在模拟生理条件(0.15 mol·L~(-1)NaCl溶液)下,应用多核(~1H、~(13)C和~(51)V)、扩散排序谱(DOSY)以及变温NMR等谱学技术研究双过氧钒配合物NH_4[OV(O_2)_2{2-(2'-Pyri-dine)- [midazole}]·4H_2O(简写为bpV(Imi-Py))和咪唑类配体(咪唑、2-甲基-咪唑、4-甲基-咪唑和组氨酸)的相互作用,其从强到弱的顺序为咪唑≈4-甲基-咪唑>2-甲基-咪唑>组氨酸.研究结果表明,咪唑环上取代基团空间位阻对反应平衡产生较大影响,同时竞争配位的结果导致新的6配位过氧物种[OV(O_2)_2L]~-(L为咪唑类配体)的生成,当配体为4-甲基-咪唑和组氨酸时,生成的则是一对异构体.To understand the substituting effects of imidazole ring on the reaction equilibrium,the interactions between diperoxovanadate complex NH_4[OV(O_2)_2{2-(2'-Pyridine)-Imidazole}]·4H_2O (abbr.bpV(Imi-Py)) and a series of imidazole-like ligands (imidazole,2-methyl-imidazole,4-methyl-imidazole,and histidine) in solution were explored using multinuclear (~1H,~(13)C,and ~(51)V) magnetic resonance,diffusion ordered spectroscopy (DOSY),and variable temperature NMR in 0.15 mol·L~(-1) NaCl ionic medium for mimicking the physiological conditions.The experimental results indicated that the activities of bpV(Imi-Py) and organic ligands were as follows:imidazole≈4-methyl-imidazole>2-methyl- imidazole>histidine.The stedc effect of the organic ligands affects the reaction equilibrium.At the same time,new six- coordinated peroxovanadate species [OV(O_2)_2L]-(L=imidazole-like ligands) were formed due to the competitive coordination between 2-(2'-pyridyl)-imidazole and the imidazole-like ligands.When the ligand was 4-methyl- imidazole or histidine,a pair of isomers was formed.国家自然科学基金(20772027);; 973子课题(2003CB716005);; 湖南省自然科学基金(06JJ30004);; 中国博士后科学基金(20070410805);; 湖南省教育厅青年项目(068028);; 卫生部(福建省)卫生教育联合攻关计划(3502220051027);; 厦门市重大疾病攻关研究基金(Wkj2005-2-019);; 固体表面物理化学国家重点实验室开放基金和湖南科技大学博士基金(E55107)资

Study on the variation of the iodine nutrition status among children aged 8 to 10 and pregnant women in Xiamen city after the implement of new standard of iodized salt supply

目的研究新标碘盐供应前后厦门市沿海8-10岁儿童和孕妇的碘营养变化情况,为下一步碘缺乏病防控策略调整提供实证依据。方法在新标碘盐供应前及食用新标碘盐后的第3、6、9个月调查厦门市翔安区8-10岁学生和孕妇的尿碘、尿比重、碘盐覆盖率、碘盐合格率、合格碘盐食用率和居民日人均食盐摄入量。结果自来水水碘含量为4.2μg/l,基线调查、第二、三次评估日人均食盐摄入量均数分别为5.7、6.9、6.9 g;基线调查、第一、第二、三次评估,8-10岁学生碘盐合格率和合格碘盐食用率都分别为97.1、93.1、98.0和98.0,盐碘中位数分别为28.7、24.7、24.0、25.3 Mg/kg,尿碘中位数分别为132.0、177.7、181.7和134.5μg/l,尿比重均值分别为1.0157、1.0196、1.0213和1.0184;孕妇碘盐合格率分别为97.8%、84.6%、84.4%和90.2%,盐碘中位数分别为27.8、25.7、24.3和23.7Mg/kg,尿碘中位数分别为103.8、128.5、138.9和100.2μg/l,尿比重均值分别为1.0123、1.0153、1.0180和1.0143;尿碘与采样季节、气候及尿比重有关,尿碘值与尿比重呈正相关关系。结论新标碘盐实施前后,8-10岁儿童碘营养总体处于适宜水平,但孕妇碘营养均不足,要采取措施加强孕妇碘营养,保障人群健康素质。Objective To investigate the variation of iodine nutrition among children aged 8 to 10 and pregnant women after the implement of new standard of iodized salt supply, and to provide evidence for the further adjustment of control strategy against iodine deficiency disease.Method Survey has been performed to acquire the urinary iodine, specific gravity, iodized salt coverage rate, iodized salt qualification rate, qualified iodized salt consumption rate and average daily salt intake among children aged 8 to10 and pregnant women in Xiamen city.It has been conducted for 3 times repeatedly with a regular interval of 3 months after the implement of new standard.Results The tap water iodine content was 4.2 μg / L.The average daily salt intake of baseline survey, second and third assessment was 5.7, 6.9 and 6.9 g respectively.The iodized salt coverage rates among children aged 8 to10 and pregnant women were all reached 100% in 4 assessments.Among children aged 8 to 10, iodized salt qualification rate and qualified iodized salt consumption rate for 4 assessments were 97.1, 93.1, 98.0 and 98.0 respectively.Median of iodine salt samples was 28.7, 24.7, 24.0 and 25.3 mg / kg, respectively.The median of urinary iodine was 132.0,1 77.7, 181.7 and134.5 μg / L,respectively.The mean of specific gravity was 1.0184, 1.0157, 1.0196 and 1.0213, respectively.Among the pregnant women, the iodized salt qualification rate and qualified iodized salt consumption rate of pregnant women were 97.8, 84.6, 84.4 and 90.2%, respectively.Median of iodine salt samples was 27.8, 25.7, 24.3 and 23.7 mg / kg, respectively.Median of urinary iodine was 103.8, 128.5, 138.9 and 100.2 μg / L,respectively.Mean of specific gravity was 1.0123,1.0153,1.0180 and 1.0143, respectively.Urinary iodine was correlated with season of sampling, climate and specific gravity, and urinary iodine was positively correlated with specific gravity.Conclusion The iodine nutrition status of children aged 8 to 10 was remained in adequate level regardless of the implement of new standard of iodized salt.However, the results suggested that the corresponding indicator among pregnant women was in a relatively low level.Proper countermeasure should be taken to enhance the iodine su-pply among them and to further secure the quality of general population

第十八届美国理论与应用力学大会总结

1会议概况2018年6月5—9日,第18届美国理论与应用力学大会(18th U.S. National Congress of Theoretical and Applied Mechanics, USNCTAM2018)在美国芝加哥召开.本次大会由美国力学国家委员会和中国力学学会联合主办,旨在探讨和交流近四年世界范围内在理论和应用力学领域的基础研究、创新技术的最新进展,吸引了来自世界各地的近千名专家学

21世纪中国金融学教学改革与发展战略

国家教育部新世纪教改工程项目《21 世纪中国金融学专业教育教学改革与发展战略研究》, 目前已取得阶段性成果。2001 年8 月16 日至21 日, 承担该项目的中央财经大学、中国人民大学、厦门大学和复旦大学在青海省西宁市举行了成果交流和研讨会。项目主持人、中央财经大学副校长、金融学博士生导师王广谦教授主持了这次会议, 全国所有有金融学博士学位授权点的高校和科研院所都有代表出席大会, 用主持人的话来说, “全国金融学界顶尖级人物基本上都到了”。出席会议的还有教育部高等教育司刘凤泰副司长和杨志坚处长。与会专家学者回顾了近20 年国内外金融业的迅猛发展, 分析了在21 世纪随着经济全球化、金融国际化对我国经济金融带来的机遇和挑战, 以及对我国金融研究、金融学科建设和人才培养带来的冲击,大家认识到, 经过20 年的改革开放和发展, 目前我国在这些领域虽有长足的进步, 但仍远远不能适应形势发展的要求。要想把我国金融学科建设成国际一流学科, 培养出一流的高素质金融专业人才, 还必须进行一系列改革与创新。与会专家教授根据各自的教学实践, 提出了许多具有建设性的意见。研讨会上发言踊跃、气氛热烈, 不时出现不同观念的碰撞和不同观点的交锋, 对推进金融学科建设十分有益。下面将著名专家教授颇具代表性的观点( 按发言顺序) 择要摘发, 以飨读者, 以推动中国金融学科的建设与繁荣

research on image blur algorithm optimization using opencl

现代GPU一般都提供特定硬件(如纹理部件、光栅化部件及各种片上缓存)以加速二维图像的处理和显示过程,相应的编程模型(CUDA、OpenCL)都定 义了特定程序设计接口(CUDA的纹理内存,OpenCL的图像对象)以便图像应用能利用相关硬件支持。以典型图像模糊化处理算法在AMD平台GPU的优 化为例,探讨了OpenCL的图像对象在图像算法优化上的适用范围,尤其是分析了其相对于更通用的基于全局内存加片上局部存储进行性能优化的方法的优劣。 实验结果表明,图像对象只有在图像为四通道且计算过程中需要缓存的数据量较小时才能带来较好的性能改善,其余情况采用全局内存加局部存储都能获得较好性能 。优化后的算法性能相对于精心实现的CPU版加速比为200~1000;相对于NVIDIA NPP库相应函数的性能加速比为1.3~5。Modern GPUs generally provide specific hardware(such as texture,grating components and various on-chip cache) to accelerate the 2D image processing and displaying process.Programming model defines specific APIs to facilitate image applications taking advantage of image-related GPU hardware,such as CUDAs texture memory and OpenCLs Images Object.Taking the optimization of image blur algorithm on AMD GPU as an example,the paper made a deep insight into the using of OpenCLs image object on image applications,especially its advantage and disadvantage compared to the more general optimization method based on global memory and the on-chip local memory.The experimental results demonstrate that the image object can provide better performance only when the processing image is four-channel and the amount of data to be cached is small.For other cases,optimizing with global memory and local memory can get better performance.After optimization,the speedup reaches 200x to 1000x in comparison with the well optimized CPU code,and the speedup over NVIDIA NPP version is upto 1.3x to 5x

基于OpenCL的图像模糊化算法优化研究

现代GPU一般都提供特定硬件(如纹理部件、光栅化部件及各种片上缓存)以加速二维图像的处理和显示过程,相应的编程模型(CUDA、OpenCL)都定义了特定程序设计接口(CUDA的纹理内存,OpenCL的图像对象)便于图像应用能利用相关硬件支持。以典型图像模糊化处理算法在AMD平台GPU的优化为例,探讨OpenCL的图像对象在图像算法优化上的适用范围,尤其是其相对于更通用的基于全局内存加片上局部存储进行性能优化方法的优劣。实验结果表明图像对象只有在图像为四通道且计算过程中需要缓存的数据量较小时能带来较好的性能改善,其余情况采用全局内存加局部存储能获得更好性能。优化后的算法性能相对于精心实现的CPU版加速比为200-1000;相对于NVIDIA NPP库相应函数的性能加速比为1.3-5。中国计算机学

基于OpenCL的归约算法优化研究

归约算法在科学计算和图像等领域有着广泛应用,本文系统研究了在OpenCL 框架下,归约算法在 GPU上的跨平台性能优化.已有研究工作一般只侧重单个硬件架构,本文基于 OpenCL 从向量化、片上存储体冲突、线程组织方式和指令选择优化等多个优化角度系统考察了不同优化方法在GPU 硬件平台的影响.具体以minMax函数为例对每种优化方法进行了详细的性能分析,并给出了提高性能的原因.在AMD GPU和NVIDIA GPU平台分别测试的结果表明优化后的算法在两个平台上都能实现很好的性能加速.在AMD ATI Radeon HD 5850平台上,Int和Float类型数据带宽利用最高达到了实测带宽的89％.在NVIDIA GPU Tesla C2050 平台上,性能也达到了CUDA版本的相应函数性能的1.3~1.9 倍.中国计算机学