Involvement of Abnormal MicroRNA Expression in the Mechanism of Drug Resistance with Acute Myeloid Leukemia

目的：分析miRNA-98的表达异常在急性髓系白血病化疗耐药机制的作用，通过靶向过表达miRNA-98的表达水平探讨以miRNA-98为靶点增加化疗药物敏感性的可能性并分析其可能作用的机制。评价异常表达的血清miRNA-155是否可作为急性髓系白血病的预后标志。 方法：离体实验中，采用qRT-PCR方法检测K562和其耐药株K562/A02中miR-98、E2F1基因表达情况。通过瞬时转染miR-98mimic处理耐药细胞K562/A0248h后，通过qRT-PCR法检测miR-98表达情况；通过qRT-PCR和Westernblot方法检测E2F1表达的变化，应用CCK8方法检测耐药细胞的...Objective：To analyze the effects of miRNA-98 on drug resistance in chemotherapy of acute myeloid leukemia(AML).To explore the possibility and mechanism of increasing the sensitivity of chemotherapeutic drugs by using miRNA-98 as a target and its overexpression. To evaluate whether the abnormal expression of serum miRNA-155 can be used as the prognostic marker in AML. Methods：In vitro...学位：医学硕士院系专业：医学院_内科学学号：2452013115346

优化海兔肝蛋白质组提取与分离技术

为了提高海兔肝脏蛋白质组的提取及研究效率,本文描述和比较了3种提取海兔肝脏蛋白质组的方法:裂解液浸泡-超速离心,丙酮-TCA沉淀-裂解液浸泡,裂解液浸泡-丙酮-TCA-裂解液溶解.然后采用常规SDS-PAGE双向电泳对提取出的蛋白质进行检验,用M elan ie 4 Trial软件分析电泳图谱,通过对蛋白质分辨率和斑点总数的比较,选出一种更适合海兔肝蛋白质的提取方法.结果表明采用丙酮-TCA沉淀-裂解液浸泡法提取海兔肝蛋白质所得的双向电泳图谱不论从蛋白质斑点分辨率还是总数上都优于其他两种方法,更适合提取分离海兔肝脏蛋白质

Association of TolC expression with resistance to tetracycline in Escherichia coli K-12

随着抗生素的开发与使用,细菌在对多种抗生素的适应过程中逐渐发展出对抗生素耐药的反应机制。TolC是药物排出转运体系的外膜成分,与AcrAB一起形成主要的药物排出泵,有关其表达量与耐药间的关系目前尚不清楚。试验将tolC克隆到pET-32a载体上进行诱导表达,镍柱纯化,免疫新西兰大白兔,获得1:4000的抗血清。Western blotting分析表明,TolC的表达量在耐四环素的大肠埃希菌K-12中比对照组提高50%。细菌TolC高表达试验发现,其MIC从100μg/ml提高到200μg/ml。结果说明TolC的表达量与四环素耐药性直接相关,提示细菌可以通过调节外膜蛋白的表达实现对抗生素的耐受。Association of TolC expression with resistance to tetracycline in Escherichia coli K-12 was studid.TolC was an outer membrane protein,which was bound with AcrAB to form a critical multidrug efflux pump.However,association of TolC amount with drug resistance was ill-defined.In the study,tolC was cloned into pET-32a vector induced expression and its recombinant proteins were purified by Ni-NTA.The purified recombinant proteins were used for preparing of anti-TolC.The prepared antiserum with titer 1:4000 was utilized as the primary antibody in Western blotting.It showed that TolC amount was increased 50% in tetracycline-resistant E.coli K-12 compared to its original control strain.When TolC was overexpressed,the strain showed strong resistance to tetracycline with its MIC from 100μg/ml to 200μg/ml.These findings indicated that TolC amount was directly correlated to bacterial resistance to tetracycline,suggesting that bacteria mounted an adaptive feedback to antibiotics by regulating outer membrane protein expression

エリート段階における中国遠隔高等教育 : 普通大学・広播電視大学の在学生・卒業生調査より

日本教育社会学会第46回大会, 1994年10月(椙山女学園大学), Ⅳ-7部会 社会発展と教育(2

EFFECTS OF SALINITY ON THE SETTLEMENT AND METAMORPHOSIS IN Styela canopus LARVAE

通过盐度梯度实验检验了盐度对冠瘤海鞘 (Styelacanopus)幼体附着和变态的影响。在盐度10～40范围内 ,低盐度抑制幼体变态 ,高盐度促进其开始变态及完全变态 ,但盐度40不促进完全变态。低盐度可使幼体附着 (除盐度10抑制附着 ) ,高盐度促进附着 ,但盐度达40不促进附着。The effects of salinity on the settlement and metamorphosis of Styela canopus larvae were investigated.Within the range of10～40,lower salinities restrain S.canopus larvae from motamorphosing,and higher salinities promote S.canopus larvae initiating metamorphosis and completing metamorphosis,but the salinity of40does not facilitate completed meta-morphosis.S.canopus larvae can settle in lower salinities(except that the salinity of10restrains settlement),and higher salinities promote settlement.But when the salinity is as high as40,settlement isn't facilitated.国家自然科学基金资助项目49976034

Ionic control of settlement and metamorphosis in the tadpole larvae of ascidian, Styela canopus Savigny

研究了人工海水中三种金属离子K+,Mg2+和Ca2+分别在不同浓度下对冠瘤海鞘(Styelaca nopusSavigny)幼体附着和变态的影响,同时采用K+通道阻滞剂TEA检验其可兴奋细胞膜上的K+通道特性,对冠瘤海鞘幼体附着和变态的离子控制机制进行初步探讨,结果表明:K+的浓度为20~40mmol/dm3 时诱导幼体附着和变态的效果最佳,低于4 5mmol/dm3 和高于80mmol/dm3 的K+浓度都表现出抑制效果;Mg2+浓度在0 ~ 32 mmol/dm3 时显著促进幼体附着和变态,达88mmol/dm3时起抑制作用;在人工海水中无论添加或减少Ca2+,均有效抑制幼体变态.TEA不能阻抑增加K+的诱导作用,这表明冠瘤海鞘幼体可兴奋细胞膜上的K+通道对外部TEA不敏感.另外,人工海水中缺乏K+或Ca2+或K+浓度达80mmol/dm3 都会对冠瘤海鞘幼体产生毒害作用.The effects of changes in concentrations of potassium, magnesium and calcium on settlement and metamorphosis in the Styela canopus Savigny larvae in artifical seawater and the characteristic of the potassium channels in externally accessible, excitable cell membrane were studied. K~(+) effectively induced larval settlement and metamorphosis during the concentrations of 20~40 mmol/dm~3, but showed inhibitive effect in the concentrations being less than or egual to 4.5 mmol/dm~3 or being greater than or egual to 80 mmol/dm~3.The inductive effect of 0~32 mmol/dm~3 Mg~(2+) was remarkable, but 88 mmol/dm~3 Mg~(2+) inhibited larval settlement and metamorphosis. The settlement and metamorphosis of the larvae would be inhibited no matter whether increased or decreased Ca~(2+) in artificial seawater. The failure of inhibiting the inductive effect of K~(+) by a K~(+)-channel blocker TEA (tetraethylammonium chloride) indicated that the potassium channels in externally accessible, excitable cell membrane of Styela canopus Savigny larvae are insensitive to external TEA. Like 80 mmol/dm~3 K~(+), K~(+)-free or Mg~(2+)-free seawater would produce evidence of toxicity. The mechanisms of ionic control of settlement and metamorphosis in the Styela canopus Savigny larvae were discussed.国家自然科学基金资助项目(49976034)

油滴铺展与聚并的数学模型

对复合驱采出液破乳过程中的某些微观现象进行了观测和研究，在复全驱采油液的破乳过程中，随着油滴在水相中上浮，会发生油滴变形铺展成油膜，油膜之间又很快发生聚并的现象，其原因是当前油滴浮出水相，作用在油滴上的表面张力发生变化，使油滴以固定的比例铺展成同膜，理论推导和实验数据都证明其比例系数是只与乳液体系的自身性质有关的常数，聚并前后的油膜半径变化规律与球状油滴聚并变化规律一样，这是对油滴按比例铺展成油

Preliminary study on chemical cues in the conspecific aggregation of Styela canopus

研究了不同浓度的冠瘤海鞘成体被囊萃取物和幼体萃取物及不同的幼体密度对冠瘤海鞘(Styelacanopus)幼体附着和变态的影响.结果表明,冠瘤海鞘被囊萃取物和幼体萃取物在5%和10%的浓度下均促进冠瘤海鞘幼体的附着和变态,但被囊萃取物的诱导效果明显强于幼体萃取物;幼体密度高于25只/cm3时,促进冠瘤海鞘幼体的附着和变态;幼体密度低于10只/cm3时,幼体的附着和变态不受密度影响.这些结果提示海区中冠瘤海鞘的同种群聚可能与其被囊或幼体内含诱导幼体附着和变态的化学因子有关.The effects of conspecific adult tunic extracts and larval tissue extracts in different concentrations and the effects of different larval density on settlement and metamorphosis in the Styela canopus larvae were investigated.Experiments show that conspecific adult tunic extracts and larval tissue extracts both were able to induce Styela canopus larvae to settle and metamorphose in the concentrations of 5% and 10%.It is found that Styela canopus larvae are induced to settle and metamorphose at densities of more than 25 larvae per millilitre and the settlement and metamorphosis are not influenced at densities of lower than 10 larvae per millilitre.All results suggest that the ability of chemical factors in adult tunic extracts or larval tissue extracts to induce settlement and metamorphosis may explain the conspecific aggregation of Styela canopus in nature.国家自然科学基金资助项目(49976034)

Influences of marine adhesive bacteria on settlement and metamorphosis of Styela conopus Savigny larvae

从冠瘤海鞘、皱瘤海鞘及乳突皮海鞘的被囊表面及其附着基和附近的海水中分离到290株细菌,根据其形态和生理生化特征,将其鉴定至弧菌属、肠杆菌属和无色杆菌属等10个属.分析海鞘被囊表面及其附着基和海水中的细菌组成,并从各属中挑出的29株细菌中分离筛选出附着能力较强的9株.将这9株细菌分别制成不同的单种细菌黏膜及9种细菌混合黏膜,检测细菌黏膜对冠瘤海鞘幼体附着和变态的影响.结果表明不同细菌黏膜对冠瘤海鞘幼体的附着和变态起不同的作用.无色杆菌属H-13显著地促进海鞘的附着和变态;弧菌属的H-4对海鞘的附着和变态均起抑制作用;弧菌属H-9明显地促进附着却强烈抑制海鞘幼体变态;其余细菌仅仅对海鞘幼体附着或变态的一个过程起作用或是对两者都没有明显效果.One hundred and eighty bacteria isolated from surface of tunics of Styela conopus Savigny, Styela plicata Lesueur, Molgula manhattensis Delay and their adhesive substrates, as well as 110 ones from nearby water, were investigated . On the basis of their morphological, biochemical and physiological characteristics, these bacteria belong to 10 genera and over 60% bacteria are Gram-negative. In bacteria from surfaces of sea squirt's tunics and their adhesive substrates, dominant colonies are Pseudomonas, while Aeromonas are primary in bacteria from water. Nine strains were screened out from 180 bacteria for their great ability of attachment. Then these 9 bacteria were cultured to form different bacterial films respectively, together with a mixed artificial film made of all 9 strains so that influences of these artificial films on settlement and metamorphosis of Styela conopus Savigny larvae were studied. It is revealed that different types of artificial films have dissimilar effects on settlement and metamorphosis of Styela conopus Savigny larvae. On one hand, 5 kinds of bacterial films, H6, H9, H13, H15 and the mixed film remarkably stimulate larva attachment, while H4 strongly inhibited and others are without evident influences. On the other hand, in 10 bacterial films, H4, H7, and H9 obviously restrain metamorphosis of lava of Styela conopus Savigny, but more larva are metamorphic when they grew on H13 and the mixed bacterial film. It illustrated that settlement and metamorphosis of Styela conopus Savigny larva were two correspondingly independent processes during these two courses, different effects were shown when larva grew on the same bacterial film.国家自然科学基金资助项目(49976034)

エリート段階における中国遠隔高等教育 : 普通大学・広播電視大学の在学生・卒業生調査より

日本教育社会学会第46回大会, 1994年10月(椙山女学園大学), Ⅳ-7部会 社会発展と教育(2