目的：分析miRNA-98的表达异常在急性髓系白血病化疗耐药机制的作用，通过靶向过表达miRNA-98的表达水平探讨以miRNA-98为靶点增加化疗药物敏感性的可能性并分析其可能作用的机制。评价异常表达的血清miRNA-155是否可作为急性髓系白血病的预后标志。 方法：离体实验中，采用qRT-PCR方法检测K562和其耐药株K562/A02中miR-98、E2F1基因表达情况。通过瞬时转染miR-98mimic处理耐药细胞K562/A0248h后，通过qRT-PCR法检测miR-98表达情况；通过qRT-PCR和Westernblot方法检测E2F1表达的变化，应用CCK8方法检测耐药细胞的...Objective：To analyze the effects of miRNA-98 on drug resistance in chemotherapy of acute myeloid leukemia(AML).To explore the possibility and mechanism of increasing the sensitivity of chemotherapeutic drugs by using miRNA-98 as a target and its overexpression. To evaluate whether the abnormal expression of serum miRNA-155 can be used as the prognostic marker in AML. Methods：In vitro...学位：医学硕士院系专业：医学院_内科学学号：2452013115346
