Progress in thermal-mechantical effects induced by laser

对激光辐照诱导的热与力学问题研究进展进行了综述,包括材料在高温、高升温速率下的本构关系,典型薄板和柱壳等结构在激光辐照下的热力破坏效应,多层材料体系的激光破坏行为等几个方面,并着重介绍了包含相变与烧蚀过程的激光破坏分析模型与机制研究,激光辐照效应的流-热-固耦合数值模拟方法,以及短脉冲激光引起的冲击与破坏机理等方面的研究新进展.</p

Influence of Particle Damper Configurations on the Dynamic Characteristic for Gear Transmission System

齿轮传动正朝着高速、重载和高精度方向发展,对其动态性能要求越来越高。颗粒阻尼通过颗粒间、颗粒与阻尼器壁间的非弹性碰撞和摩擦作用耗能,具有减振效果; 显著、耐高温、各向同性、对原结构改动小等优点。利用有限元法对齿轮进行了有预应力的模态分析,通过齿轮系统动力学分析,研究单双齿啮合激励对齿轮传动的; 影响;同时建立齿轮传动离心场中颗粒系统耗能模型,将离散元法计算颗粒系统耗能和试验测试对比,分析阻尼器配置对齿轮传动系统动特性的影响。结果显示,阻; 尼器配置方案是影响齿轮传动系统动特性的一个重要因素,在阻尼孔中填充一定数量的颗粒,当阻尼器总体积相同且齿轮结构静刚度相差不大时,阻尼器个数越多颗; 粒系统能耗越大;当阻尼孔直径相同时,阻尼孔个数越多颗粒系统总能耗越小;当阻尼孔个数相同时,阻尼孔直径越大颗粒系统能耗越小。通过试验和仿真对比验证; 了模型的正确性,为颗粒阻尼在离心场中的应用提供重要的理论依据。The gear transmission is advancing towards high speed, heavy load and; high precision. The requirements for the dynamic performance of gear; system are urgently proposed. The particle damping dissipates mechanical; system energy through inelastic collisions and friction between; particles. It is an effective and simple measure for vibration; reduction. It has many advantages, such as isotropy, high temperature; resistance and less modification to the original structure. This paper; has conducted the prestressed modal analysis of gear system by the; finite element method. Based on the dynamics analysis of gear system,; the effect of single tooth and double teeth meshing incentive on gear; transmission has been analyzed. The energy dissipation model of particle; damping for gear transmission in centrifugal field has been established.; By contrasting theoretical analysis and the test, the effect of damper; configuration on dynamic characteristic of gear transmission system is; analyzed. The results show that damper configuration is an important; factor of the dynamic characteristic for gear transmission system.; Filling a certain number of particles, when the gears static stiffness; are similar and the total dampers volume are equal, the more damping; hole, the greater the total energy loss. When the damping holes diameter; are the same, the more number of damping hole, the smaller the total; energy loss. When the damping hole number is the same, the bigger the; diameter, the less the total energy loss. Experimental results are; consistency with the theoretical analysis. These results can provide; guidelines for the application of particle damping in centrifugal field.国家自然科学基金; 福建省重点科技专项; 中央高校基本科研业务费专项资金; 福建省高端装备制造协同创新中心资助项

柱层析和MALDI-TOF质谱技术筛选食道癌血清标志多肽

目的探索建立柱层析和基质辅助激光解吸电离飞行时间(MALD I-TOF)质谱技术筛选食道癌血清标志多肽的方法。方法选用Sephadex G-100葡聚糖作为分离介质,自制小型分离层析柱,分别采集30名健康人、30例食道良性疾病患者和30例食道癌患者血清进行多肽分离,结合MALD I-TOF质谱仪鉴定。结果通过多肽质荷比(m/z)比对技术,筛选出4种诊断食道癌疾病的潜在血清标志多肽,m/z分别为7 651.76、8 091.34、9 264.98、11 784.79。结论柱层析和质谱技术筛选食道癌血清标志多肽所建立的分析技术不仅快速简单,而且同样适合于筛选其他人类重大疾病的血清标志多肽或酶蛋白

Baseline-free damage identification of metallic sandwich panels with truss core based on vibration characteristics

A baseline-free damage identification method is proposed to identify damages in metallic sandwich panels with truss core in the article. The method is based on flexibility matrix and gapped smoothing method, with damage index defined DIm. The weight coefficient m is introduced to consider the effect of damages on both low-order modes and high-order modes. Numerical simulations and experiments are conducted to evaluate the present method. Besides, damage index DIm* is also defined by processing DIm with Teager energy operator, and comparisons between DIm and DIm* are also carried out. Results show that the proposed method is effective in detecting single damage and multiple damages of the same or different extent. The weight coefficient m plays a very important role in identification of multiple damages of different styles. When comparing with DIm*, it is found that the present index DIm is better at suppressing the singularity caused by contact nodes and detecting of multiple damages which contain small or slight damages.</p

反应温度和pH影响魟鱼肝铁蛋白释放铁速率的研究

小批量制备电泳纯魟鱼肝铁蛋白(liver ferritin ofDasyatis akajei,DALF).在不同反应温度和pH条件下,研究温度和pH对DALF释放铁速率、动力学和级数转换特性的影响.实验结果表明,随着反应温度递增(30~45℃),DALF释放铁的速率明显不同,但DALF释放铁的全过程均呈复杂途径.通过提高反应温度和增加释放铁的速率途径均无法使DALF释放铁的途径由复杂转换为简单.不同pH的反应介质直接影响DALF释放铁的速率,在弱酸介质中,DALF释放铁的速率明显高于在弱碱介质中.在pH5.0条件下,DALF以一级反应动力学的全过程途径释放铁,使释放铁的复杂途径趋于简单化.在生理pH或中性介质条件下,铁蛋白亚基之间相互作用强度起着控释DALF释放铁速率且表现出复杂动力学特性

基质辅助激光解吸电离飞行时间质谱技术研究人血清转铁蛋白稳定性及裂解产物

制备质谱纯人血清转铁蛋白(HTF),供分子结构分析。选用SDS-PAGE、胶外酶解、基质辅助激光解吸/电离质谱技术(MALDI-TOF)、数据库检索和比对技术鉴定铁饱和HTF、双铁HTF(HTF-2Fe3+)、单铁HTF(HTF-Fe3+)和脱铁HTF(apoHTF)的稳定性和裂解产物。以乙腈溶液作为洗脱相,发现铁饱和HTF在RP-HPLC分离纯化过程中产生裂解现象。铁饱和HTF和HTF-2Fe3+经乙腈处理后均能产生不同分子量的短肽裂解产物,指出HTF结构稳定性与络合铁离子数量有关。铁组分改善了HTF分子结构的稳定性。采用比对法,研究在乙腈作用下HTF裂解成为各种各样短肽的规律,初步阐明其裂解机理。在乙腈作用下,HTF可能通过蛋白质去折叠途径,形成不同多聚态HTF或多肽裂解产物。推测目前用于临床诊断先天性糖基化紊乱(CDG)和慢性酒精滥用(CAA)疾病低准确率的起因可能是受HTF裂解产物或多聚体的干扰

质谱技术在筛选肺癌血清差异多肽与蛋白质的应用

采用凝胶排阻层析(SEC)与MALD I-TOF质谱非在线联用技术研究男性血清中多肽与蛋白质的组成与分布.实验结果表明,分离介质Sephadex G-75比Sephadex G-50和Sephadex G-100更加适合血清多肽与蛋白质的预分离.男性血清经Sephadex G-75层析柱预分离和MALD I-TOF质谱分析后,可获得56个多肽与蛋白质的分子离子峰.差异比对实验表明,在正常人与患有肺癌疾病的男性血清中,正常男性血清存在着7种不同质荷比(m/z)的差异多肽与蛋白质,其m/z的比值分别为4 808,6 867,6 912,7 036,27 990,28 120,50 687;反之,在肺癌患者血清中发现差异的m/z值为6 139,6 544,8 417,8 480,11 109.这些差异多肽与蛋白质为临床诊断正常人和患有肺癌疾病患者的标记物提供一条途径

α-羟丁酸脱氢酶与乳酸脱氢酶比值的临床意义

目的探讨α-羟丁酸脱氢酶与乳酸脱氢酶比值对心肌梗死的诊断价值。方法采用470例血清HBDH升高患者血清,分为心肌梗死组、脑部疾病组、肺部疾病组、肝脏疾病组、肾脏疾病组,测定血清中α-羟丁酸脱氢酶与乳酸脱氢酶比值。同时,设正常对照组。结果心肌梗塞组及脑部疾病组HBDH/LDH比值明显升高,与正常对照组有着显著性差异(P0.05)。结论以HBDH/LDH的比值诊断心肌梗塞较之单纯用HBDH或LDH诊断特异性高