Intermolecular Multiple- Quantum Coherences and Their Applications on Biological Tissues

高分辨核磁共振(nuClEAr MAgnETIC rESOnAnCE,nMr)波谱是一种能够在非侵入性状态下获得生物组织分子信息的有效手段。分子间多量子相干(InTErMOlECulAr MulTIPlE-QuAnTuM COHErEnCE,IMQC)信号源于分子间的远程偶极相互作用,仅依赖于偶极相关距离内的磁场相对均匀性,因此可用于在不均匀磁场下获得高分辨的nMr谱。文中简要介绍了IMQC的发展历程及现状,并主要讨论了IMQC方法及其在活体及生物组织中的应用。虽然IMQC目前应用在老鼠身上比较常见,但最近的研究表明IMQC方法已被用于人体小脑的研究,将来IMQC方法将有可能应用于人体的其他器官以及其他的动物研究,具有广阔的发展前景。High resolution nuclear magnetic resonance(NMR) spectroscopy offers an effectively noninvasive method to obtain molecular information in biological tissues.Intermolecular multiple- quantum coherences(iMQCs) originating from distant dipolar interactions between spins just rely on the relative homogeneity of magnetic field within the dipolar correlation distance.Therefore,iMQC can acquire high- resolution NMR spectroscopy in inhomogeneous fields.In this review,the development history and current situation of iMQC were briefly introduced,and the applications of iMQC on in vivo biological tissues were mainly discussed.Although it is more common to apply iMQC in mice /rats,recent investigation indicated that iMQCs can also been used in the study of human cerebellums.In the future,iMQC may be applied to study other organs of human and other animals,indicating that it is a promising method.国家自然科学基金资助项目(项目编号:11105114

Study on the Interaction of Gliotoxin with BSA

应用荧光、圆二色和紫外—可见吸收等波谱法研究胶毒素与牛血清白蛋白(bSA)的相互作用。荧光光谱实验结果表明胶毒素主要靠疏水作用与bSA结合,而对其内源荧光产生猝灭作用,其淬灭方式为静态猝灭,胶毒素与bSA的结合常数为7.2x103l/MOl。圆二色光谱检测发现,随着胶毒素浓度的增加,bSA的α-螺旋数量也增加,当胶毒素浓度为bSA浓度的100倍时,bSA的α-螺旋增加40.1%,表明胶毒素与bSA的结合改变了bSA的空间构象。The interaction between Gliotoxin and bovine serum albumin (BSA) was studied by the fluo-rescence, Circular Dichroism (CD) and ultraviolet visible (UV-Vis) techniques.The fluorescent experiment showed that the intrinsic fluorescence of BSA was quenched by the binding of gliotoxin in a static quenching procedure, with an association constant of 7.2×103 L/mol and in hydropobic forces.And the CD spectrum revealed that gliotoxin effected the conformation of BSA by increased the mass of α-helix.国家863计划项目(No.2006AA09Z410

Biological fixed nitrogen of Tokin Gulf in winter

2006年12月至2007年1月(冬季)期间,对北部湾海区的生物固氮作用进行了初步研究,结果表明:当培养时间为2 H时,水体浮游生物固氮速率最高,且固氮速率随着培养时间的延长而逐渐降低。北部湾冬季固氮速率存在周日变化,8∶10~11∶10时段内固氮速率达到最高值。10 M和30 M水深在夜间仍能检测到固氮活性,这可能是浮游生物昼夜垂直移动引起的。北部湾冬季固氮速率的范围为447.5~1447.2 PMOl/(l.H),固氮速率呈现出从湾内往湾外不断增加的趋势,湾口海区(b06站)的积分固氮速率为319.5μMOl/(M2.d)。b06站fE加富实验表明,添加100 nMOl/l fE能够明显促进生物固氮作用,北部湾冬季湾口海区生物固氮作用可能受到fE限制。The biological nitrogen fixation rates were investigated in Tokin Gulf waters in winter from Dec.2006 to Jan.2007.The nitrogen fixation rates were decreased as extension of culture time,the optimal culture time was 2 h.The nitrogen fixation rates showed diurnal variations,the highest nitrogen fixation rate appeared at 8∶ 10--11∶ 10,the diazotrophs still have the nitrogen fixation activity during the night at the depths of 10 m and 30 m,which may result from the diurnal migration of planktons.The nitrogen fixation rates ranged from 447.5 pmol/(L·h) to 1 447.2 pmol/(L·h) of Tokin Gulf waters in winter.Which showed a longitudinally increasing trend,the integral nitrogen fixation rate at station B06 was 319.5 μmol/(m2·d).The iron enrichment experiment at the mouth of the bay(B06) showed that the biological nitrogen fixation significantly promoted by adding iron concentration of 100 nmol/L.The biological nitrogen fixation rates at the bay mouth might be iron limited.国家自然科学基金重大研究计划重点项目资助(90411016);国家教育部高等学校骨干教师计划基金资助项

Development of aquantitative ELISA detection method for Varicella Zoster Virus(VZV) antigen

目的:建立水痘-带状疱疹病毒(VzV)抗原的双抗体夹心ElISA定量检测方法,用于质控VzV灭活疫苗研发和生产中抗原含量。方法:以VzV中和单抗5f6C8为包被抗体、8H5d1为酶标抗体,构建定量检测VzV抗原的双抗体夹心ElISA方法,并对本方法的特异性、灵敏度、准确性、线性和稳定性等性能进行分析。结果:建立的双抗体夹心定量检测VzV抗原的ElISA方法,线性范围为0.4μg~13μg/Ml,相关系数为r2=0.994,定量限度为0.4μg/Ml;变异系数CV80%。与VzV以外的相关病毒样本没有交叉反应。结论:构建的VzV抗原ElISA定量检测方法的各项性能符合定量检测需要,可用于VzV灭活疫苗的研发和生产过程的抗原含量检测。Objective:To develop a quantitative enzyme linked immunosorbent assay(Q-ELISA) to determine the concentration of Varicella Zoster Virus(VZV) antigen.This method was used to determine VZV antigen content at each stage of VZV inactived vaccine developing and manufacturing process.Methods: A double antibody sandwich Q-ELISA was developed to determine concentration of VZV antigen,which was based on the the high-affinity neutralizing monoclonal antibodies 5F6C8 as capture antibodies,and 8H5D1 as HRP-labeled antibody.The performance of reagent were evaluated.Results: The Q-ELISA for VZV antigen content was successfully developed.The reagent had good performance.The quantitation scope was 0.4 μg~13 μg/ml,The coefficient correlation was 0.994,the limit of detection was 0.4 μg /ml,the recovery was between 87.5% and 111.6%.The stability was up to 80% after reagent was heated for 6 days at 37℃.The variation coefficient was lower than 15%,and the reagent was no reaction with other sample except VZV antigen.Conclusion: The Q-ELISA for VZV antigen was developed with good specificity,accuracy and stability.The method can be used to determine VZV antigen content during development and production of VZV inactived vaccine

纪念五四宪法颁布暨全国人民代表大会成立五十周年理论研讨会纪要

今年 9月是五四宪法颁布、全国人民代表大会成立五十周年。五四宪法是新中国的第一部宪法 ,为我国的社会主义民主法制奠定了基础 ,具有划时代的意义。坚持和完善人民代表大会制度 ,是新的历史条件下推进政治体制改革和社会主义政治文明建设的重要内容。基于“以学术表达纪念、以纪念促进学术”的目的 ,中国社会科学院法学研究所于 2 0 0 4年 9月 5日举办了“纪念五四宪法颁布暨全国人民代表大会成立五十周年理论研讨会”。来自全国各地的 70余位代表莅临会议 ,研讨了诸多热点和前沿问题

Distribution of Symbiotic and Epiphyte Antibiotic Microorganisms Associated with Marine Organisms in the Intertidal Zone of Xiamen Sea Area

研究厦门海区潮间带海洋动植物共附生抗菌微生物的分布.结果表明,海洋动植物体表或肠道存在细菌10--4~10--5个/g、真菌10--3~10--5个/g和放线菌10--3~10--4个/g.其菌量随宿主而变化;在289株分离菌株中,53株菌株有抑菌活性,28株为细菌,17株为放线菌,8株为真菌,30%的拮抗菌可抑制3种以上敏感菌的生长;鉴定结果表明,拮抗细菌以革兰氏阴性菌为主,放线菌为链霉菌和小单孢菌,真菌分属于6个不同属。本研究结果提示在与海洋动植物共附生的微生物中存在着丰富的抗菌资源。The purpose of this work is to study distribution of symbiotic and epiphyte antibiotic-roducing microorganisms associated with marine organisms in the intertidal zone of Xiamen sea area.The number of microorganisms vary with the hosts, and in general there are bacteria 104--105 cells/g, fungi 103--105 cells/g and actinomycetes 103-- 104 cells/g in the surface or intestine of marine organisms studied.Among the 289 strains isolated, there are 53 strains having an antimicrobial activity, in which 28 strains belong to bacteria, 17 to acti-nomycetes and 8 to fungi.About 30% of the strains of antibiotic ?producing microorganisms isolated could inhibit the growth of at least 3 sensitive strains tested.The main antibiotic ?producing bacteria are Gram negative whereas the antibiotic - producing actinomycetes belong to Streptomyces and Micromonospora and the antibiotic -roducing fungi belong to 6 different genera.The results of this work indicate that there exists a rich resource of antibiotic ?producers in the symbiotic and epiphyte microorganisms associated with marine organisms.国家自然科学基金资助项目(编号:39670015

Antimicrobial activity of symbiotic and epiphyte microorganisms on marine organisms in intertidal zone of Xiamen

从厦门海区潮间带石莼、浒苔、江蓠、海兔、海葵及鲨鱼肠道分离得289株共附生微生物，以8种敏感菌为指示菌，从中获得有拮抗活性的细菌28株、放线菌17株、真菌8株。拮抗细菌以假单胞菌（39%）、弧菌（25%）和芽孢杆菌（14%）为主，主要为抗革兰氏阳性细菌（占总桔抗菌的78.6%），抗真菌的菌株比例较低（占总拮抗菌的39.3%）。拮抗放线菌分属于链霉菌（88%）和小单孢菌（12%），其抗革兰氏阳性菌、阴，比菌和真菌的菌株比例分别为65%、47%和23%。拮抗真菌分属于青霉属和芽技零属等6属，仅抑制细菌的生长。The 289 strains of the symbiotic and epiphyte microorganisms were lsolated from marineorganisms (Ulra, Enteromorpha, Gracilaria, Actinia, Mustelus, and Aplysia)collected from theintertidal zone of Xiamen.Among them, 28 strains of bacteria, 17 strains of actinomycetesand 8 strains of fungi have shown to have antagonistic activity on bacterial or fungal growth.the main antibiotic-producing bacteria belong to Pseudomonas (39% ), Vibro (25%) andBacillus (14%).The number of bacterial strains, that inhibits the growth of G+ bacteria, isthe largest and the number of bacterial strains that inhibits the growth of fungi,is the least.The antibiotic-producing actinomycetes respectively belong to Streptomyes (88%) and Micromonospora (12%), 65% of strains inhibits the growth of G+ bacteria,47% for G- bacteriaand 23% for fungi.The antibiotic-producing fungi, belonged to 6 different genera, could only inhibit bacterial growth.国家自然科学基金!3967001