我国985高校外文电子图书馆藏现状调研与利用分析

以985高校图书馆为研究对象,结合电子图书在世界范围内的出版状况,对985高校图书馆外文电子图书的馆藏现状及如何揭示电子图书馆藏进行调研,以期了解外文电子图书数据库在我国高校的发行概况,把握我国985高校图书馆外文电子图书数据库的订购情况及揭示程度。“外文电子图书馆藏的模式与策略”(课题编号:K82004)课题系列研究成果之一(受Springer公司资助

政治学视角下科举解额制与高考分省录取制之比较

公平是科举制度的第一块基石,但现实社会并不具备实现绝对公平的土壤,古代统治者便采取了科举解额制等一系列科举法,来保障选仕的公平性。当前,普通高等学校统一招生考试为了实现公平,也因袭古法实行分省录取。虽然科举解额制和高考分省录取制度对追求公平的努力是一脉相承的,但是其社会功能各异,所以两种制度涉及的利害关系也不尽相同

确保馆藏信息交流与转换的一致性—— 美国馆藏说明标准述评

美国馆藏说明标准历经近40年的发展，发展较为成熟且修订经验丰富，但其发展过程并非一帆风顺，历经新旧标准的更迭、 两个标准的统一、标准的多次修订及再确认这样一个复杂漫长的过程。拟以美国馆藏说明标准原始文本为基础，结合翻译过程中的扩展研究与思考，从美国馆藏说明标准的发展历史、主要内容和特点、与其他标准之间的关系及数据项和数据元四个方面进行系统的梳理与分析，以期为我国馆藏说明标准的完善提供有益参考。国家社科基金项目“中美电子资源国家标准比较研究”(项目编号:16BTQ027)研究成果之

灾害护理课程的设计与实践

2009-2010 > Academic research: refereed > Publication in refereed journalVersion of RecordPublishe

Research progress of high voltage electrolyte for lithium-ion batteries

随着对锂离子电池能量密度要求的提高,新一代高电压锂离子电池和与之匹配的高电压电解液的研究开发也日益受到重视。从优化电解液的组成和使用具有特殊功能的新型溶剂、添加剂等方面对锂离子电池高电压电解液的研究进行分析和总结,并对未来的应用前景进行展望。With the increasing requirements for the lithium-ion battery energy density, more and more attentions are focused on the research and development of a new generation of high voltage lithium-ion battery and high voltage electrolyte.The recent research progress related to high voltage electrolyte solutions was analyzed and summarized from the electrolyte composition, special solvent and additives

Analysis of the Content of Stevia Sweeteners by Capillary Zone Electrophoresis

本文介绍了一种用毛细管区带电泳法筛选甜菊糖苷突变体的有效方法。根据实验结果 ,优化的电泳条件为 :60mmol/LTris 硼酸缓冲液 (pH 8.0 ) ,柱温 30℃ ,工作电压 2 5kV。优化条件下 ,甜菊苷 (Ste vioside)迁移时间的R .S .D为 0 .45% (1 5次 ) ,且在 7.45× 1 0 - 5～ 1 .74× 1 0 - 2 mol/L的浓度范围内存在良好的线性关系 (r=0 .9994) ,甜菊主要糖苷在 5min内均可实现分离。在优化条件下 ,本实验研究了低能离子注入后甜菊主要糖苷含量变化 ,结果令人满意。This paper introduced an effect capillary zone electrophoresis method for screening of stevia mutation. According to the experimental results, the optimum separation conditions were selected as: 60 mmol/L Tris tetraborate buffer of pH 8.0, 30 ℃ and 25 KV. Under optimum conditions, the R.S.D. of stevioside migration time for 15 runs was 0.45%. The detector response for stevioside was linear over the range of 7.45×10 -5 ～1.74×10 -2 mol/L (r=0.9994). Stevia main sweeteners cound be separated effectively in less than five minutes. This method was applied for determining the variation of stevia main sweeteners after low_energy ions implantation

Development of Electrochemical Biosensor for Detection of PML/RARα Fusion Gene in Acute Promyelocytic Leukemia

针对急性早幼粒细胞白血病(APl)中PMl/rArα融合基因的碱基序列,设计了锁核酸(lnA)修饰的发夹结构捕获探针,结合信号探针构建新型的“三明治“电化学传感模式。信号探针末端修饰的生物素可与酶上的亲和素结合,通过检测酶催化H2O2氧化底物3,3',5,5'-四甲基联苯胺(TMb)产生的电化学信号,实现对靶序列的检测。该传感器可识别和定量检测PbS缓冲液中人工合成的PMl/rArα融合基因序列。结果表明,该传感器能很好地区分互补序列、单碱基及多碱基错配序列,杂交电流值与目标链浓度在1.0x10-11~1.6x10-10 MOl/l范围内呈较好的线性关系,检出限为1.0x10-13 MOl/l。同时,该新型传感器成功地用于无稀释人血清中PMl/rArα融合基因的检测,具有特异性强、灵敏度高和重复性好的优点,有望用于临床实际样品的检测,进而实现临床上急性早幼粒细胞白血病的早期诊断及预后判断。A novel DNA electrochemical probe(locked nucleic acid,LNA) was designed and involved in constructing an electrochemical DNA biosensor for the detection of PML/RARα fusion gene in acute promyelocytic leukemia(APL).This biosensor was based on a "sandwich" detection strategy,which involved a pair of LNA probes,e.g.hairpin capture probe and reporter probe.Streptavidin-HRP was bound to biotin labeled at the end of reporter probe via streptavidin-biotin affinity binding.In the presence of hydrogen peroxide(H2O2),HRP catalyzed the oxidation of the substrate 3,3′,5,5′-tetramethylbenzidene(TMB) to offer an enzymatically amplified electrochemical current signal for the detection of target DNA.This sensor was applied in the direct quantitative detection of synthetic PML/RARα fusion gene in PBS buffer.The results indicated that the biosensor showed an excellent specificity to distinguish the complementary sequence and different mismatch sequences.A linear relationship between the amperometric signal and the target concentration was obtained in the range of 1.0×10-11-1.6×10-10 mol/L with a detection limit of 1.0×10-13 mol/L.In addition,the biosensor was used for the determination of PML/RARα fusion gene in human serum samples without dilution with high sensitivity,selectivity and good repeatability.This method would be expected to use in real sample for further solving the actural problems of early diagnosis and prognosis monitoring of APL.863计划资助项目(2008AA02Z433);福建省高校产学研科技重点项目(2010Y4003);国家自然科学基金资助项目(20805006;20975021);福建省自然科学基金资助项目(2010J05019