海西对台光电产业产学研合作现状及趋势

海峡西岸对台光电产业产学研合作是两岸科技界高度重视的一项重点工程,在两岸政策的引导下,在市场需求及产业界、学术界的共同推动下,合作领域越发广泛,合作交流的形式和渠道不断拓宽,带动两岸科技进步及经济发展。本文着重分析了两岸光电产业的发展现状和海西对台光电产业产学研合作现状、趋势,阐明了合作机制及相应的目标与重点、方式等。在此基础上提出了海峡西岸经济区对台光电产业合作的策略及对两岸光电产业良性发展的建议。福建省科技计划项目（2016R0091）;福建省高等教育教学改革研究项目（JAS151398）

Fas ligand and Anti-human DR5 monoclonal antibody induce tumor cell lines apoptosis

目的探讨FasL、Anti-DR5mAb对肿瘤细胞Hela、BGC823、MCF-7、L342、H9101、D6的杀伤作用及机制。方法采用RT-PCR、MTT比色法、电泳、DNA倍体分析、Westernblot等方法。结果H9101、Hela细胞株DR5mRNA水平有表达,D6细胞无表达;H9101、L342细胞株FasmRNA水平有表达,D6细胞无表达。H9101、L342细胞株对FasL、Anti-DR5mAb敏感并呈剂量依赖性;MCF-7、BGC823细胞株对FasL敏感,对Anti-DR5mAb相对敏感。Hela对FasL相对敏感,对Anti-DR5mAb敏感;D6对两种凋亡诱导剂耐受。结论FasL、Anti-DR5mAb能不同程度地诱导肿瘤细胞凋亡,其机制与Fas、DR5、Caspase-8、Bcl-2的表达有关。 【英文摘要】 Objective:To study the cytotoxic effects on tumor cell lines Hela,BGC823,MCF-7,L342,H9101,D6 induced by Fas ligand and anti-human DR5 monoclonal antibodies(Anti-DR5 mAb) and the underlying mechanism.Methods:Fas/DR5 mRNA were detected by RT-PCR. Cytotoxicity exerted by FasL/Anti-DR5 mAb on tumor cell lines was measured by MTT colorimetry and the induced apoptosis was determined by agarose gel electrophoresis.Results:The expression of DR5 on BGC823 and Hela cells were higher and DR5 didn’t express in D6. The ...厦门大学科研启动基金(No.Z03103)资

Testing System of Phosphor Excited by Blue LED Based on Integral Sphere

为尽可能反映荧光粉在实际工作条件下的发光性质,准确测量荧光粉的发光性能,提出了一种基于积分球的荧光粉发光性能测试系统。该系统采用蓝光lEd作为激发光源,配置有TEC控温系统对光源有效实施控温,可提供持续稳定的激发荧光粉发光。采用出光筒控制光源方向和积分球收集光线,能有效防止能量损失,提高测试的精确性。通过实验得到了不同强度蓝光激发下的荧光粉光谱功率分布以及发光效能、量子效率、光转换效率等参数的变化规律。随着驱动电流的增大,由于蓝光芯片内量子限制斯塔克效应,从而导致蓝光峰值波长出现小幅度的蓝移。三种效率在小电流下基本呈线性下降趋势,且在大电流下趋于平缓。实验结果表明,该系统及方法可以有效地评价实际lEd芯片工作状态下的荧光粉发光性能。A testing system for phosphor luminous characteristics based on integral sphere is proposed so as to show luminous characteristics of phosphor at actual operation circumstances and measure its luminous characteristics accurately.Blue LED is used as an excited source in the system.TEC temperature control system is used to control the temperature of the source effectively.And continuous and stable excited light is provided to excite phosphor illumining.Energy loss is prevented effectively and testing accuracy is enhanced for the direction of source controlled by a light tube and light ray collected by integral sphere.Based on experiments,spectrum power distributions of phosphor excited by blue light with difference light strength and variation rules of parameters such as luminous efficiency,quantum efficiency and light conversion efficiency are got.With the increasing of driving current,a small blue shift appears in blue light peak wavelength for quantum confined Stark effect in blue light chip.Three kinds of efficiency almost show linear decline trend at low current and then approach to constant at high current.Experimental results show that luminous characteristics of phosphor on real LED chip at operation state can be evaluated effectively by the system and method.国家自然科学基金项目(11104230); 福建省重点科技项目(2011H6025;2012H0039

Gonad shafting hormone level quantitative analysis and clinical significance for the patients with premenopausal breast disease

目的:研究绝经前(滤泡期或称卵泡前期)的女性乳腺增生和乳腺癌患者HPO轴系激素表达,包括激素雌二醇(E2)、促卵泡激素(FSH)、促黄体激素(LH)、孕酮(P)、睾酮(T)和促催乳素(PRL)6种,为乳腺增生和乳腺癌提供诊断依据。方法:采用放射免疫法检测正常人、乳腺增生和乳腺癌患者各62例血浆E2、FSH、P、T和PRL水平(E2、P和T单位为μg/L,FSH和LH单位为I U/L,PRL单位为g/L),并应用逻辑回归与判别分析等方法进行统计分析。结果:乳腺癌、乳腺增生症和正常人3组人群的激素分布不同,乳腺癌患者的E2(192.59)、FSH水平增高(24.25),LH(30.235 3)与E2、FSH存在协同性增高,Logistic回归分析和聚类分析发现,T水平乳腺癌组低于乳腺增生与正常人群组,差异有统计学意义,P<0.05。P和PRL水平越高,患乳腺癌的危险度上升。结论:通过回归分析和聚类分析,可能提高临床应用性激素检测判别疾病的能力及危险度预测,判别准确率为91.2%。OBJECTIVE: To investigate the HPO shafting including E2,FSH,LH,P,T and PRL in the mammary gland hyperplasia and breast cancer of premenopausal(follicle period or prophase) female patients,and provide bases for mammary gland hyperplasia and breast cancer diagnosis.METHODS: By the radiative immune method,the blood levels of E2,FSH,LH,P,T and PRL(the unit of E2,P and T was μg/L,the unit of FSH and LH was IU/L,and the unit of PRL was g/L) were detected in 62 normal persons,62 patients with mammary gland hyperplasia and 62 patients with breast cancer.Logistic regression analysis and discriminance methods were used for the identification of the results.RESULTS: The hormone distributions in the mammary gland hyperplasia patients,breast cancer patients and normal people were diffrerent.By logical regression analysis and discriminance methods,the levels of E2(192.59) and FSH(24.25) in the breast cancer group increased,while LH(30.235 3) increased cooperativly with the increase of E2 and FSH.The level of T hormone in the breast cancer group was significantly lower than that in the mammary gland hyperplasia group and the normal people group,P<0.05.The higher of P and PRL levels were,the higher the breast cancer risk was.CONCLUSION:The use of logistic regression and distinguish analysis are favorable methods in the diagnosis of mammary gland hyperplasia and breast cancer.The total accuracy is 91.2%.厦门市科技社会发展计划基金(3502Z20044003

长喙毛茛泽泻的花器官发生

用扫描电镜对长喙毛茛泽泻（ＲａｎａｌｉｓｍａｒｏｓｔｒａｔｕｍＳｔａｐｆ．）的花器官发生进行了观察。该种的花器官发生在泽泻科中既典型又比较特殊。其特殊之处在于从花原基的发生直至最外轮６枚雄蕊原基的发生过程都存在单向发育：３个萼片原基和３个花瓣原基的发生存在次序性，萼片原基和花瓣原基均为顺时针发生，邻近花序侧生分支复合芽的萼片原基和花瓣原基倾向于优先发生；最外轮６枚雄蕊的发生是成对的按对萼的形式发生。这是以往对该属花器官发生研究没有注意到的。由雄蕊原基向心皮原基的转变发生在与最外轮６枚雄蕊相交替的６个位点

死亡受体5胞外区域的重组、表达及活性鉴定

目的构建死亡受体5(deathreceptor5,DR5)胞外区域(eDR5)的表达载体,表达纯化重组蛋白并鉴定其生物特性。方法通过重叠PCR获得DR5胞外段编码序列,构建pET-22b(+)/DR5表达载体,转化大肠杆菌BL21(DE3),IPTG诱导表达,Ni2+柱亲和纯化,SDS-PAGE、直接ELISA鉴定纯化产物的纯度和特异性,用MTT法检测eDR5蛋白阻断DR5单克隆抗体FMU1.5和TRAIL诱导人胶质瘤细胞株U343(高表达DR5)、U373(低表达DR5)细胞凋亡的作用。结果获得了DR5胞外段编码序列,目的蛋白在上清及包涵体中都有表达,表达量占菌体总蛋白的30%以上,纯化的重组蛋白纯度达95%以上,蛋白产量达9mg/ml。ELISA结果表明所纯化蛋白为eDR5。eDR5蛋白可部分阻断FMU1.5和TRAIL诱导人胶质瘤细胞株U343细胞凋亡的作用,其阻断率与DR5表达相关。结论死亡受体5胞外段基因的成功重组、表达及纯化,为进一步的功能研究奠定了基础

Thermal characteristic analysis of high-power LEDs by structure functions

运用电学法测量功率型lEd冷却瞬态温度曲线,通过数学方法将其转化为积分和微分结构函数来分析器件各区域的热阻和热容,结果发现,各层材料的测量值与理论值基本一致。1μS的瞬态数据采集精度和高的重复性保证了实验结果的准确性和可靠性,运用这种方法比较了3种不同金属芯印刷电路板(MCPCb)对功率型lEd的散热效果,贝格斯Al基板散热性能最好,AnTAl基板次之,普通Al基板最差。研究表明,利用结构函数分析功率型lEd的热特性是一种强有力的方法。The cooling transient temperature curves of high-power LEDs are measured by electritical method.The cumulative and differential structure functions are extracted from these curves to analyse thermal resistances and thermal capacitances of all regimes of high-power LEDs with numeric computational method.It is found out that calculated and measured values of various materials are essentially conformable.The sampling resolution of 1 μs of transient data and high repetition assure the veracity and reliability of experimental result.Subsequently,thermal conduction capabilities of three different metal core printed circuit boards(MCPCBs) with high-power LEDs are compared by this method,and it is discovered that bergquist′s MCPCB has the best thermal conduction capability,ANT′s MCPCB takes second place,and the common MCPCB is the worst.So the structure functions are powerful tools for thermal characteristic analysis of high-power LEDs.国家“863”计划资助项目(2006AA03A175);福建省科技项目(2006H0092;2008J0030);厦门市重大专项资助项目(3502Z20061004

The Effect of Multi-irreversibilities on Thermoeconomic Performance of a Heat Transformer

【中文文摘】基于一类不可逆三热源热变换器的循环模型,提出以每单位成本的泵热率作为热经济学目标函数,优化热变换器的各种性能参数.分析内不可逆性、热漏和经济参数对热变换器性能的影响,利用数值解计算热经济学目标函数的最大值及所对应的各种性能参数. 【英文文摘】The heating load per unit cost is taken as the thermoeconomic objective function with the cycle model of a class of irreversible three-heat-source heat transformers as the basis. The objective function is used to optimize the performance of the cycle. The effect of internal irreversibility, heat leak and economic parameter on the performance of the cycle is analyzed. The maximum thermoeconomic function and the corresponding performance parameters are obtained by using numerical calculation. The results obtained here provide some valuable guidance for the optimal design and operation of practical systems.国家自然科学基金资助项目(59976033

Mechanism of geniposide in improving free fatty acid metabolism in rats with non-alcoholic fatty liver disease

观察栀子苷改善非酒精性脂肪性肝病的效果,从游离脂肪酸探讨栀子苷调节非酒精性脂肪性肝病的作用机制。健康雄性Wistar大鼠40只,随机分为4组:正常组、模型组、栀子苷组和血脂康组,每组大鼠10只,正常组大鼠给予正常大鼠饲料喂养,其余3组大鼠均采用高脂饲料喂养以诱导非酒精性脂肪性肝病,造模时间为8周,从第5周起至第8周末,栀子苷组和血脂康组分别灌服相应的药物。记录大鼠体重、肝湿重、脂肪质量;用相应方法检测肝组织TG,FFA,FAS,AMPK,ACCase及Malonyl-Co A含量,血清CHO,LDL-C的含量,血清AST,ALT的活性;观察肝脏组织肉眼及病理变化(HE染色法)。结果显示,与正常组相比,模型组大鼠的体重;肝湿重;脂肪重;血清CHO,LDL-C,ALT,AST;肝组织TG,FFA,FAS,ACCase及Malonyl-Co A含量皆显著升高(P<0.01);肝组织AMPK活性显著降低(P<0.01),肝组织肉眼外观及病理切片脂肪变性明显,并出现炎症损伤;与模型组相比,栀子苷组大鼠体重、脂肪质量、肝组织FFA含量、血清ALT,AST活性均显著降低(P<0.01),肝湿重,肝组织TG,FAS,ACCase及Malonyl-Co A含量明显降低(P<0.05),肝组织AMPK活性明显增多(P<0.05),肝脏肉眼外观及病理学表现均有所改善;与模型组相比,血脂康组大鼠的肝湿重、脂肪质量、肝组织TG,FFA和血清LDL-C水平明显降低(P<0.05);与血脂康组相比,栀子苷组大鼠的体重、脂肪质量、肝组织FFA含量均显著降低(P<0.01),其他方面无明显差异。结果表明,栀子苷具有显著的改善高脂饮食诱导的大鼠非酒精性脂肪性肝病的药理效应;其改善大鼠非酒精性脂肪性肝病的游离脂肪酸代谢是通过调节"AMPK-ACCase-Malonyl-Co A-FFA"轴来实现的。To observe the effect of geniposide on non-alcoholic fatty liver disease( NAFLD),and discuss the mechanism of geniposide for NAFLD from the aspect of free fatty acid,forty healthy Wistar male rats were randomly divided into normal group,model group,geniposide and Xuezhikang group. The rats in normal group were fed with normal diets,and the rats in other 3 groups were given with high-fat diet for 8 weeks to induce the NAFLD models. From the week 5 to end of week 8,the rats in geniposide and Xuezhikang group were intervened with corresponding medicines. The body weight,liver wet weight,and fat weight of the rats were recorded.Visual and pathological changes in hepatic tissues were observed with HE staining. The contents of TG,FFA,FAS,AMPK,ACCase and Malonyl-Co A in hepatic tissue,contents of CHO and LDL-C in serum and activities of AST and ALT in serum were detected by using corresponding methods. The results showed that the body weight,liver wet weight,and fat weight of the rats,CHO,LDL-C,ALT and AST levels in serum,TG,FFA,FAS,ACCase and Malonyl-Co A levels in hepatic tissues of the rats in model group were significantly higher than those in normal group( P < 0. 01),while AMPK activity was significantly lower than that of the normal group( P < 0. 01),with obvious visual and pathological steatosis in hepatic tissues,and inflammatory injury occurred in model group. Compared with the model group,body weight of the rat,fat weight,levels of FFA in hepatic tissues,ALT and AST activities in serum,liver wet weight,TG,FAS,ACCase and Malonyl-Co A levels were significantly decreased in geniposide group( P < 0. 01),while the AMPK activity in hepatic tissues was significantly increased( P < 0. 05),with improvement in visual and pathological performance.Compared with the model group,liver wet weight,fat weight,TG and FFA levels in hepatic tissues,and LDL-C level in serum were significantly decreased in Xuezhikang group( P < 0. 05). Compared with Xuezhikang group,the body weight of rat,fat weight and FFA level in hepatic tissues were significantly lower in geniposide group( P < 0. 01),but with no significant difference in other aspects. These findings indicated that geniposide was highly effective in improving the pharmacological effect of NAFLD induced by highfat diet,and the mechanism was achieved through AMPK-ACCase-Malonyl-Co A-FFA axis.国家自然科学基金项目(81274155;81503529);; 福建省自然科学基金项目(2014J01374);; 福建省高等学校新世纪人才计划项目;; 厦门市科技计划项目(3502Z20134020);; 福建省中医药科研项目(WZPW201308

在福建东南沿海局部地域献血员中检出HTLV-I

[目的 ]进一步阐明福建沿海 HTL V小流行区 HTL V- 的地理分布特点及毒株的基因类型。 [方法 ]对福建东南沿海莆田地区献血员以国产双抗原夹心法 EL ISA试剂筛选 ,对 EL ISA阳性血清用 Western blot(WB)进行确证。对抗体确证阳性者 ,采集外周血分离淋巴细胞用巢式 PCR扩增 HTL V- env区 gp46片段进行序列分析鉴定病毒亚型。 [结果 ]从 1998年 10月至 2 0 0 0年 4月止 ,共检测 45 6 4份标本 ,结果确证出 16例 HTL V- 抗体阳性者 ,感染率为 0 .35 %。HTL V- 抗体阳性者主要分布在某村献血人群中 ,并具有区域集中的特点。笏石秀屿一带某村献血员中感染率高达 4.7%。7例代表性毒株经病毒序列分析为 HTL V- C亚型 (COSMOPOL ITAN)。[结论 ]福建东南沿海某村献血员中发现 HTL V- 流行。提出在对某一地区人群进行 HTL V筛选时 ,既要注意整个地区“面”的筛查 ,又要注重某个村镇“点”的调查