均匀设计法筛选茵陈蒿汤抗脂肪肝脂质代谢异常的效应组分

目的:运用均匀设计法筛选茵陈蒿汤抗脂肪肝脂质代谢紊乱的效应组分及其最优剂量配比,探讨揭示复方配伍理论科学内涵的研究方法。方法:将茵陈蒿汤(茵陈、栀子、大黄)进行分组设计,分为6种中药组合,对高脂饮食诱导的大鼠脂肪肝模型进行干预,观察各组大鼠肝脏的病理变化,并以血清三酰甘油(TG)、肝组织TG、血清低密度脂蛋白(LDL)测定作为筛选指标,分析不同作用途径的效应组分及其最优剂量配比。结果:较之正常组,模型组血清TG、LDL和肝组织TG含量均明显升高(P<0.05~0.01),肝组织存在明显脂肪变性;各药物组上述脂肪病理改变程度均明显减轻。根据均匀设计实验逐步回归分析,降低血清TG和降低肝组织TG的效应组分分别是茵陈、栀子。而茵陈与大黄对降低血清LDL有明显疗效,以茵陈0.3g+大黄0.15g为最优剂量组合。结论:茵陈蒿汤通过多途径调节脂肪肝的脂质代谢,其不同作用途径存在特定的效应组分及相应的最优剂量配比

股市泡沫的生成机理和度量

本文首先系统地回顾了有关股市泡沫的研究成果 , 指出股市泡沫研究中的三大难题 , 即股市泡沫定义、度量和成因; 接着简要地介绍股市泡沫的度量模型和检验方法 , 并联系我国实际 , 根据资本资产定价模型 (CAPM) 和市盈率定价方法 (Pricing by Price - Earning Ratio) 提出一个股市泡沫的度量模型; 最后根据理性预期理论分析理性泡沫的生成机理 , 同时根据信息不对称、反馈理论和选美博弈分析非理性泡沫的生成机理 , 得出我国股市泡沫更多的表现为非理性泡沫的结论 , 并进一步分析其主要成因

舌鳞癌微血管密度与预后的关系

【目的】研究舌鳞状细胞癌(简称舌鳞癌, 下同)组织中新生微血管的密度(MVD)与舌鳞癌患者预后的关系。【方 法】用免疫组织化学S-P 法对75 例舌鳞癌标本进行染色, 依据Weidner 法进行肿瘤微血管密度计数, 并与舌鳞癌的病理分级、 颈淋巴结转移与否及患者生存期作比较。【结果】不同分化程度舌鳞癌组织中新生血管密度不同(P <0.01), 低分化者 M VD 高于高分化者;G1 , G2 , G3 期MVD 分别为24±10, 31 ±11 和48 ±12 ;颈淋巴结转移组MVD 为36 ±11 , 无颈淋巴结转移 组MVD 为27±9(t =2.383, P <0.01);五年生存组MVD 为26 ±9, 五年内死亡组MVD 为34±10(t =2.042, P <0.01)。 【结论】肿瘤微血管密度与舌鳞癌生物学行为相关, 可以作为舌鳞癌患者预后的指标

Analysis on the characteristics of macrobenthis community in the North-west Daya Bay of South China Bay in spring

利用2008年春季在大亚湾西北部海域开展的12个测站的大型底栖动物调查资料,对该海域大型底栖动物的群落特征进行分析。结果表明该海域内大型底栖动物优势种由粗帝汶蛤(TIMOClEA SCAbrA)、波纹巴非蛤(PAPHIA undulATA)、上野蜾蠃蜚(COrOPHIuM uEnOI)、方背鳞虫(lEPIdOnOTuS SQuAMATuS)、刀明樱蛤(MOErEllA CulTEr)和角海蛹(OPHElIA ACuMInATA)等6种组成;可划分为4个群落,群落Ⅱ和群落Ⅰ为主体群落,群落Ⅲ和群落Ⅳ分布区较小,群落间差异较大。大型底栖动物数量上以个体较大的软体动物占优,但群落的组成则以多毛类和甲壳类为主,其组成情况基本上反映了群落的特征。群落Ⅰ以多毛类-甲壳类为表征,群落Ⅱ则以甲壳类-多毛类-软体动物为表征。栖息环境的差异,是造成群落间差异的主要原因;群落多样性水平呈群落Ⅲ>群落Ⅱ>群落Ⅰ>群落Ⅳ,沉积环境的污染状况和人为扰动是影响多样性水平的主要因素;群落整体的稳定性较好,未受到明显的扰动,但分布于航道区域和渔业生产作业区的群落Ⅳ和群落Ⅲ,在航道建设、疏浚和渔业生产的影响下,群落受到一定程度的扰动,稳定性较弱。Based on the data collected from 12 research stations in the North-west Daya Bay,South China Sea in spring 2008,the characteristics of macrobenthis community were examined.The results showed that the dominant macrobenthic species were Timoclea scabra,Paphia undulate,Corophium uenoi,Lepidonotus squamatus,Moerella culter and Ophelia acuminate.The macrobenthos community could be divided into four groups with notable differences.Group Ⅰ and Ⅱ were the dominant groups,while the distribution areas of Groups Ⅲ and Ⅳ were relatively small.Overall,molluscs was dominant in quantity in the macrobenthos community but the community was mainly composed of Annelida and Arthropoda,reflecting the characteristics of the community.The dominant macrobenthic species of group Ⅰ were Timoclea scabra,Nephtys oligobranchia,Theora lata,Sigambra hanaokai,Glycinde gurjanvae,Prionospio malmgreni,Timoclea scabra,Turritella bacillum,Corophium tridentium,Phyllodocidae und.,Nectoneanthes alatopalpis,Ericthonius pugnax,Pseudopythina sp.and Stylochus ijimai.Species belonging to this group distributed in the nearshore area of Aotou Harbor and Nanhai Petrochemicals Basement Group Ⅱ assemblage includes Paphia undulata,Timoclea scabra,Corophium uenoi,Prionospio malmgreni,Sigambra hanaokai,Ophelia acuminata,Mediomastus sp.,Nephtys oligobranchia,Moerella culter,Corophium lamellatum,Paraprionospio pinnata and Prionospio pulchra.These species distributed mainly around the Chunzhou Island where suspended long line culture of oyster was common.The discriminating species for Group Ⅰ were S.hanaokai,N.soligobranchia,P.malmgreni,N.alatopalpis and E.pugnax while group Ⅱ were C.uenoi,P.malmgreni,S.hanaokai,N.oligobranchia,T.scabra,M.sp.,Phyllodocidae und.,P.pinnata,O.acuminata,C.lamellatum,M.culter and Chaetozone sp.Group Ⅲ assemblage were mainly found in the west side of Mabianzhou,where it was a fishing ground for Arthropoda in Daya Bay.Group Ⅳ was distributed in area where main shipping routes passed through,in the east side of area of Group Ⅱ and in between the area of Group Ⅰ and Ⅲ.For each community group,while group Ⅱ Group Ⅰ was dominated by Annelida and Arthropoda while Group Ⅱ was dominated by arthropoda,annelida and mollusca;such difference is mainly because of the difference in habitat conditions.Diversity of macrobentho community was highest in Group Ⅲ,followed by Group Ⅱ,Group Ⅰ and then Group Ⅳ.Poor habitat condition caused by pollution,sedimentation and other human disturbances were the main reasons for the differences in diversity between groups.In general,macrobenthos community in the North-west Daya Bay of South China Sea was stable in spring.However,community structure of Groups Ⅳ and Ⅲ,which distributed in the main navigation route and fishing grounds,were less stable;suggesting the disturbances from maintenance of navigation channel and fishing activities might have reduced community stability.中央级公益性科研院所基本科研业务费专项基金(2007ZD08;2007TS08;2010YD10

Separation and Purification of Naringin by Solid Phase Extraction Using Strong Anion Exchange Cartridge

作者简介：宁方刚(1986 —)，男，硕士研究生，研究方向为天然产物化学。E-mail：[email protected] * 通信作者：许建中(1954 —)，男，高级工程师，本科，研究方向为生物化工。E-mail：[email protected][中文文摘]目的:建立固相萃取分离纯化柚皮苷的方法。方法:以强阴离子交换(strong anion exchange,SAX)填料为吸附材料,利用柚皮苷与填料的功能基团之间的静电作用将柚皮苷吸附在填料上,考察洗脱液离子强度、洗脱液体积,洗脱流速对洗脱效果的影响。结果:选用5mL碳酸氢钠溶液(0.1mol/L,pH9.0)为洗脱液,以0.5mL/min的流速进行洗脱,回收率达到91%以上。结论:本方法简单、高效,选择性好,可为柚皮苷样品的纯化提供理论依据。 [英文文摘]Purpose: A solid-phase extraction procedure for the separation and purification of naringin(NG) was developed using strong anion exchange(SAX) cartridge.Method: Naringin was adsorbed onto the cartridge by electrostatic interaction between naringin and functional groups.Several variables affecting the elution performance of SAX,such as ionic strength of elution,elution volume and elution rate,were studied.Results: The application of 5 mL of sodium bicarbonate solution(0.1 mol/L,pH = 9.0) to elute SAX cartridge at a flow rate of 0.5 mL/min resulted in a recovery rate of 91%.Conclusions: The established method is simple and efficient.福建省科技计划重点项目(2010Y0035

Determination of Trace Metal Elements in Crude Oils by Microwave Digestion-Inductively Coupled Plasma-Mass Spectrometry Assisted with Organic Solvent

李景喜( 1980 － ) ，男，山东临沂市人，研究实习员，从事分析化学研究。E-mail: jxli@fio．org． cn。[中文摘要]建立了二氯甲烷溶剂辅助微波消解-电感耦合等离子体质谱法测定原油中V、Cr、Fe、Ni、Cu、Zn、Mo、Co、Pb等16种微量金属元素。研究表明,用CH2Cl2分散原油样品,以浓HNO3-H2O2为消解体系,采用CEM微波消解系统消解样品,各元素线性关系良好,相关系数≥0.9995;检出限可达ng/L;方法精密度较高,相对标准偏差(RSD,n=3)<5.0%;回收率为92%～110%。以w(Ni)/w(V)与w(Fe)/w(V)比值为变量参数对不同原油样品进行聚类分析,表明国内与国外不同地区原油样品中各金属元素含量差异较大。[英文文摘]A method for determination of V,Cr,Fe,Ni,Cu,Zn,Mo Co,Pb,etc in different crude oils was established by inductively coupled plasma-mass spectrometry assisted with organic solvent microwave digestion.The oil sample was digested by microwave using HNO3-H2O2 as oxidant after dispersing in organic solvent(CH2Cl2).The results showed that the detection limits of the method reached ng/L level for sixteen elements.The linear correlation of the method was preferable with the correlation coefficient of better than 0.9995． The recoveries of the method were in the range of 92% ～ 110% with the precision of less than 5.0% RSD (n=3)．The ratios of w(Ni)/w(V) and w(Fe)/w(V) were distinct in different crude oil samples from oversea and domestic，and the index can be used to the cluster analysis for differencing the crude oil samples．国家海洋局第一海洋研究所基本科研业务费专项资助(2010G23);海洋公益性行业科研专项资助(200705011);海洋溢油鉴别与损害评估技术重点实验室开放基金资助(200920

Successive modification of polydentate complexes gives access to planar carbon-and nitrogen-based ligands

以碳和氮为键合原子的多齿螯合物是配合物家族中非常重要的一类。具有更高齿数的平面构型NC螯合物实例相对较少，代表性的例子为四齿金属碳卟啉类化合物，这类螯合物以其独特的结构和丰富的物理化学性质引起广泛关注。然而平面五齿、六齿的NC螯合物由于几何构型“拥挤”导致合成难度高，该工作从含三元环内金属卡宾结构的CCCC型碳龙配合物出发，利用经典有机反应（炔烃对金属卡宾的插入反应），成功地实现了CCCCN/NCCCN型平面五齿螯合物的合成。这一研究为高配位型螯合物的合成提供了新思路并为平面五齿螯合物家族添加新成员。特别是，这些高配位型螯合物在可见光和近红外区域均有较好的吸收，表现出良好的光声成像、光热转换及声动力学性能。 该研究工作在张弘教授指导下完成，第一作者为iChEM博士后周小茜。该工作充分体现了多学科协同研究优势：相关化合物的合成、表征及理论计算工作由周小茜博士完成；声动力学性能研究由厦门大学公共卫生学院庞鑫博士及刘刚教授完成；光声成像研究由厦门大学公共卫生学院聂立铭教授完成。iChEM fellow卓庆德博士、博士生卓凯玥、陈志昕参与了部分实验工作。夏海平教授、香港科技大学林振阳教授和南京大学朱从青教授对研究工作给予了大力支持。【Abstract】Polydentate complexes containing combinations of nitrogen and carbon (N and C) ligating atoms are among the most fundamental and ubiquitous molecules in coordination chemistry, yet the formation of such complexes with planar high-coordinate N/C sites remains challenging. Herein, we demonstrate an efficient route to access related complexes with tetradentate CCCN and pentadentate CCCCN and NCCCN cores by successive modification of the coordinating atoms in complexes with a CCCC core. Combined experimental and computational studies reveal that the rich reactivity of metal-carbon bonds and the inherent aromaticity of the metallacyclic skeletons play key roles in these transformations. This strategy addresses the paucity of synthetic approaches to mixed N/C planar pentadentate chelating species and provides valuable insights into the synthesis of carbon-based high-coordinate complexes. Furthermore, the resulting complexes are the examples of organometallic species with combined photoacoustic, photothermal, and sonodynamic properties, which makes them promising for application in related areas.This research was supported by the National Natural Science Foundation of China (Nos. 21572185, 21561162001, and 81571744), the Research Grants Council of Hong Kong (N_HKUST603/15), the Excellent Youth Foundation of Fujian Scientific Committee (2018J06024), and the Fundamental Research Funds for the Central Universities (20720170065).该工作得到国家自然科学基金委、香港研究资助局、福建省自然科学基金、厦门大学校长基金的资助

城乡链接与农民合作

2009-2010 > Academic research: refereed > Publication in refereed journalVersion of RecordPublishe

CCCCC pentadentate chelates with planar Möbius aromaticity and unique properties

本课题充分发挥了厦门大学多学科协同研究优势，通讯作者为夏海平教授（合成、表征）、刘刚教授（生物医学应用）和吕鑫教授（理论计算）。合成实验和结构表征由朱从青（第一作者，目前在麻省理工学院、2005年诺贝尔化学奖得主Richard Schrock教授课题组从事博士后研究）完成；生物医学应用由杨彩霞（共同第一作者）、林凎、杨宇惠、王晓勇合作完成；理论计算由朱军、王永恒、朱从青完成。美国NIH的陈小元教授参与了生物医学应用的讨论。该研究工作得到国家自然科学基金委、科技部项目的支持。The coordinating atoms in polydentate chelates are primarily heteroatoms. We present the first examples of pentadentate chelates with all binding atoms of the chelating agent being carbon atoms, denoted as CCCCC chelates. Having up to five metal-carbon bonds in the equatorial plane has not been previously observed in transition metal chemistry. Density functional theory calculations showed that the planar metallacycle has extended Craig-Möbius aromaticity arising from 12-center–12-electron dπ-pπ π-conjugation. These planar chelates have broad absorption in the ultraviolet-visible–near-infrared region and, thus, notable photothermal performance upon irradiation by an 808-nm laser, indicating that these chelates have potential applications in photothermal therapy. The combination of facile synthesis, high stability, and broad absorption of these complexes could make the polydentate carbon chain a novel building block in coordination chemistry.the National Basic Research Program of China (nos. 2012CB821600 and 2014CB744503) , the National Science Foundation of China (nos. 21332002, 81422023, 51273165, 21490573, and 21573179)