Microbial Transformation of Gypenoside from Gynostemma pentaphyllum by Aspergullus glaucus and Its Biological Activities

以福建绞股蓝[Gynostemma pentaphyllum(Thunb)Makino]为材料,经乙醇提取和正丁醇萃取获得绞股蓝皂苷,利用灰绿曲霉(Aspergullus glaucus)微生物转化绞股蓝皂苷,通过测定绞股蓝皂苷和灰绿曲霉转化产物的抗癌、抗酪氨酸酶和抗氧化活性,比较绞股蓝皂苷经微生物转化修饰前后生物活性的差异.结果表明:灰绿曲霉转化产物对肝癌细胞SMMC7721有体外抑制作用,半抑制质量浓度(IC50)为91.66μg/m L,而绞股蓝皂苷抗癌效果不强;绞股蓝皂苷对蘑菇酪氨酸酶活力具有较强的抑制作用,IC50为0.14 mg/m L,其抑制作用属于可逆抑制,抑制类型为混合型抑制作用,而灰绿曲霉转化产物抗蘑菇酪氨酸酶活性较弱;绞股蓝皂苷和灰绿曲霉转化产物分别在0.2和2 mg/m L质量浓度下对DNA氧化损伤有保护作用.研究结果说明绞股蓝皂苷经微生物修饰后,抗癌效果增强,对蘑菇酪氨酸酶抑制作用和DNA氧化损伤保护作用减弱.该研究结果可为利用微生物转化法筛选抗癌绞股蓝皂苷奠定基础.Gynostemma pentaphyllum in Fujian Province was used as the material in this research.Gypenoside was extracted with ethanol and n-butyl alcohol. Gypenoside was microbially transformed by Aspergullus glaucus. The abilities of anticancer,anti-tyrosinase and anti-oxidation in gypenoside and transformation products were measured in our study.The difference of bioactivity between gypenoside and microbially transformed gypenoside was compared.The result showed that the products of transformation by A. glaucus affected the growth of hepatocellular carcinoma cells SMMC7721 with half maximal inhibitory concentration( IC50) values of 91. 66 μg/m L,but the anticancer activity of gypenoside was very weak.Gypenoside had strong inhibition to the mushroom tyrosinase. The value of IC50 was 0. 14 mg / m L. Moreover,the products of transformation by A. glaucus showed little anti-tyrosinase acti-vity.The kinesis study showed that the inhibition of gypenoside to mushroom tyrosinase was reversible and inhibition types were mixed. Gypenoside exhibited the active protective effect on H2O2-induced oxidative-stress damage at the concentration of 0. 2 mg / m L,and the products of transformation by A. glaucus were at 2 mg / m L. This research indicated that the anticancer effect of gypenoside modified by microorganisms was enhanced,but the activity of anti-tyrosinase and H2O2-induced oxidativestress damage became weakened.This study laid the foundation of screening anticancer gypenoside by means of microbial transformation.国家自然科学基金(81274149);; 厦门市科技创新项目(3502Z20132009,3502Z20154083);; 厦门市对台科技合作项目(3502Z20141041

A Study on Electrochemical Activity and ECL Properties of Some Bioactive Peptides

探讨了某些生物活性肽的电化学和电致化学发光特性的联系.The relativity of the peptides′ electrochemistry active and chemiluminescence intensity information are investigated.The electrochemiluminescence behavior of some bioactive peptides was studied by FIA-ECL.These peptides′ electrochemistry active at the glassy carbon electrode was also studied using a BAS-100 model electrochemical analyzer.国家自然科学基金!（296750003）;福建省自然科学基金!（B9810006

试论民间审计人员的法律责任

试论民间审计人员的法律责任陈汉文，池晓勃一、产生民间审计人员法律责任的社会原因任何一种职业，其应承担的责任与其社会地位之间有着直接的联系。对审计人员来说，只有当准备承担责任并对因未能满足规定的要求而引起的后果负责时，其地位和执业能力才会被认可。审计人..

The Establishment of a FIA System with ECL Detection

讨论了一种流动注射电致化学发光检测系统的研制， 并用luMInOl 对其性能进行了测试.A novel ECL detection system for HPLC and FIA has been set-up.The performance of our detection system has been studied with luminol.国家自然科学基金!（296750003）;福建省自然科学基金!（B9810006

Determination of Glutathione by FIA with ECL Detection

发现氧化型谷胱甘肽能增强钌联吡啶的电致化学发光， 而还原型谷胱甘肽抑制钌联吡啶的电致化学发光. 根据增强或抑制程度的大小， 可定量测定谷胱甘肽.We reported the determination of glutathione based on electrogenerated chemiluminescence in the presence of ruthenium compound.The various parameter influencing the ECL-FIA of peptides as electroysis pulse parameters and medium conditions were optimized indetail.The sensitivity is sufficient.国家自然科学基金!（296750003）;福建省自然科学基金!（B9810006

关于环境审计的几个问题探讨

关于环境审计的几个问题探讨陈汉文，池晓勃环境审计，是一项由审计部门对政府及企事业单位的环境管理责任进行的鉴证活动。人们对它的称谓不一，诸如：环境回顾、考查、调查、质量控制、环境评估和绿色审计等，对其历史发展、理论基础及主要内容等方面的理解也相异。本文..

Late Quaternary Sediment Stratigraphy and Marine Cycles in the Pingtan Island, Fujian Province

选择位于福建平潭岛北部芦洋埔海积平原的PT01钻孔,岩芯长度28.3 m,采用加速器碳同位素(AMS 14C)和光释光(OSL)测年建立地层年代框架,认为晚更新世富含有孔虫等海相生物化石的地层应归属于MIS 5高海面期,即MIS 5e的潮下带滨海相沉积。结合岩性特征、古生物化石、沉积物色度、粒度分析及其参数等指标,进行了沉积单元的划分,重建了MIS 6末期以来的沉积相变化过程,揭示出MIS 5期间的多次海平面波动变化,即MIS 5高海面期沉积环境仍存在滨海、潮坪、潟湖、风成砂等沉积相之间的转换,并可能存在短暂的陆相或风化过程。而该区整个末次冰期的沉积缺失或较薄,上部全新世沉积也不发育,厚度仅为3.79 m,以沼泽相―风成砂相为主。A 28.3 m long core, PT01, from the Pingtan Island, Southeast China, is selected to be studied. Radiocarbon dating(AMS 14C) and optical stimulated luminescene(OSL) are used for establishing chronology. Combining age-dating results with marine microfossil and color index, we classify the Late Quaternary sediment stratigraphy in the coastal region of Fujian Province. It is suggested that the late Pleistocene marine deposit, dated back to 124 ka, and abundant marine microfossil(e.g. foraminifer and ostracods), would belong to the Marine Isotope Stages 5(MIS 5). Moreover, the late Quaternary sediment could be divided into three units based on the analysis of lithology, microfossil, grain size and color index. Our results reveal that the sediment environment during the MIS 5 was significantly controlled by global sea level change which varied among coast, tidal flat, lagoon, and eolian sand, and probably there existed weathering process. A hiatus during the last glacial(MIS 2-4) was attributed to low relative sea level. And the Holocene deposit, as thick as 3.79 m, developed poorly and was mainly comprised of swamp facies and eolian dune.国家自然科学基金(41230101、41072128

养殖对虾一种新杆状病毒的研究

应用超薄切片、负染色和蔗糖梯度超离心技术,在自然发病和人工感染的对虾肝、肠、淋巴样器官、鳃和肌肉组织中,发现并纯化了一种新杆状病毒.该病毒直径96~112nM,是已知对虾病毒中最粗的一种.中央是高电子密度的核心,外裹衣壳和囊膜,两膜之间有宽阔的间隙,这是已报道的任何一种对虾杆状病毒所没有的.纯化的病毒核衣壳表面有螺旋排列的亚单位,这也是本病毒特有的.主要靶细胞是淋巴样细胞,未在肝、肠和鳃上皮细胞中观察到,这与对虾杆状病毒无一例外地侵染某一种上皮细胞迥然不同.病毒仅在靶细胞核内增殖,不形成封入体或包涵体,它是一种迄今未曾报道的新病毒,称之为“淋巴样细胞核型杆状病毒”.国家“八五”科技攻关;福建省水产厅市重大科技资助项

对虾口服虾康素后对一些生理指标的影响

本文对对虾口服虾康素后的血细胞密度、血液蛋白质、耐受低溶解氧能力、饲料消化能力和蜕壳频率进行测定分析。结果显示对虾血细胞密度和血液蛋白质含量均比对照组提高,其中部分组别的血细胞密度与对照组形成极显著的差异。口服药物对血液蛋白质的影响早于对血细胞密度的影响。对虾的耐低溶解氧能力在用药组也有所增强,但药物剂量增大时,耐低溶解氧能力又接近对照组。用药组对饲料的摄食强度比对照组高50%以上。而排出的粪便含蛋白质量仍比对照组低。此外,用药各组在试验过程中蜕壳尾数明显多于对照组