DNA content detection, Differential proteomic study and expression analysis of pstB in Microcystis aeruginosa PCC 7806 cultured on different N/P nutrients

铜绿微囊藻(Microcystisaeruginosa)是富营养化湖泊和水库中形成“水华”的主要藻种。利用分子生物学技术和蛋白质组学，研究常见赤潮、水华藻种在特定环境中的蛋白质组差异，从中寻找出有价值的差异蛋白质分子，这在理论和实践上对水华的防治都具有重要意义。 在水华蓝藻的形成因素中，氮磷比例是一个很大的影响因素，其最适N/P为16：1。我们设想，该N/P值是与细胞的生长分裂相关系的。磷在细胞中的作用是参与一些代谢途径的磷酸化反应，更重要的可能是与细胞DNA、RNA的合成有关；氮则是参与了蛋白质的合成，氮磷之间合适的比例，就代表了细胞生长与分裂之间量的关系。为了验证此设想，通过流式细胞术P...Microcystis aeruginosa is the main cyanobacteria, which can form “water bloom” in lakes and reservoirs.So use the technologies of molecular biology and proteomics to study valued differential proteins expressed under specific conditions, which will either in theory or in practice pour great effect on the prevention of algae bloom. In the formation of algae bloom, the proportion of nitrogen and...学位：理学硕士院系专业：生命科学学院生物学系_生物化学与分子生物学学号：2172006115220

DNA content detection of microcystis aeruginosa PCC 7806 on different N/P nutrients by FCM

在蓝藻水华的形成因素中,氮磷比例是一个很大的影响因素,其最适n/P为16∶1。因此笔者设想,该n/P值是与细胞的生长分裂相关系的。磷在细胞中的主要作用与细胞dnA、rnA的合成有关;氮则是参与了蛋白质的合成,氮磷之间合适的比例,就代表了细胞生长与分裂之间量的关系。为了验证该设想,通过流式细胞术P I染色法来检测微囊藻细胞的dnA含量,设置不同的n/P比值,缺nP、1∶1、16∶1、160∶1四组,结果表明在n/P为16∶1时,藻细胞的dnA含量较其他对照组高,间接地反映了藻细胞的数目,即当n/P为16∶1藻细胞处于分裂的最佳水平。In the formation of algae bloom,the proportion of nitrogen and phosphorus is the major influential factor.The optimal N/P for alage is 16:1.It is assumed that the particular proportion be related to the growth and division of cyanobacteria cells.The most vital role in vivo of phosphorus is involved in DNA、RNA synthesis.As for nitrogen,it is related to the protein synthesis.The appropriate ratio between N and P may be presumed as a reflection of the relation in quantity between the growth and division of alage cell.In order to verify the assumption,we used the technology of FCM to detect the cells of M.aeruginosa stained by PI which were cultured on different N/P nutrients and set up four groups of different N/P ratios,16∶1,160∶1,1∶1 and lacking of NP.It was found that when N/P=16∶1,the DNA content of M.aeruginosa cells is higher than others.We concluded that the division of these cells is at an optimal level

天山北部沙漠－绿洲－冰雪带的水热循环与环境效应研究

该项目利用树木年轮重建了天山中段北坡370年的降水日数序列,分析了树木年轮与非常规积温的关系。项目揭示了新疆极端降水的变化和空间分布差异,得出天山南北麓绿洲的冬、夏和年极端降水量都有不同程度的增多趋势,年极端降水量于1980年发生了突变。在天山北坡四工河流域绿洲—沙漠交界处进行了野外大气观测实验,研究了天山北坡中部经向剖面上的沙漠—绿洲—高山冰雪气候带气候变化随高度的响应,在各个高度上,冬季增温幅度都要大于夏季;在沙漠的低海拔地区,年降水量增加幅度并不是最大的,而在海拔较高的山前绿洲地带和在3500m的高山区降水量增幅相对较大。项目研究发现隆冬时期低纬系统异常活跃,在一定的大气环流条件下,阿拉..

中国脑血管病临床管理指南（第2版）（节选）——第3章 脑血管病高危人群管理 Chinese Stroke Association Guidelines for Clinical Management of Cerebrovascular Diseases (Second Edition) (Except) ——Chapter Three Management of Patients at Hige-risk of Cerebrovascular Diseases

针对脑血管病的危险因素积极进行早期干预管理是减少脑血管病危害最有效的方法。本章节介绍脑血管病高危人群管理的推荐意见，包括对不可干预和可干预血管危险因素的控制，阿司匹林在缺血性卒中一级预防中的应用以及首次卒中风险评估等方面。 Abstract: Early intervention to manage the risk factors of cerebrovascular disease is the most effective way to reduce the harm of cerebrovascular diseases. This chapter presents recommendations for the management of populations at high risk for cerebrovascular disease, including control of non-interventionable and interventionable vascular risk factors, the use of aspirin in the primary prevention of ischaemic stroke, and the risk assessment of first-time stroke

JUNO Sensitivity on Proton Decay p→νˉK+p\to \bar\nu K^+ Searches

The Jiangmen Underground Neutrino Observatory (JUNO) is a large liquid scintillator detector designed to explore many topics in fundamental physics. In this paper, the potential on searching for proton decay in $p\to \bar\nu K^+$ mode with JUNO is investigated.The kaon and its decay particles feature a clear three-fold coincidence signature that results in a high efficiency for identification. Moreover, the excellent energy resolution of JUNO permits to suppress the sizable background caused by other delayed signals. Based on these advantages, the detection efficiency for the proton decay via $p\to \bar\nu K^+$ is 36.9% with a background level of 0.2 events after 10 years of data taking. The estimated sensitivity based on 200 kton-years exposure is $9.6 \times 10^{33}$ years, competitive with the current best limits on the proton lifetime in this channel

JUNO sensitivity on proton decay p → ν K + searches*

The Jiangmen Underground Neutrino Observatory (JUNO) is a large liquid scintillator detector designed to explore many topics in fundamental physics. In this study, the potential of searching for proton decay in the $p\to \bar{\nu} K^+$ mode with JUNO is investigated. The kaon and its decay particles feature a clear three-fold coincidence signature that results in a high efficiency for identification. Moreover, the excellent energy resolution of JUNO permits suppression of the sizable background caused by other delayed signals. Based on these advantages, the detection efficiency for the proton decay via $p\to \bar{\nu} K^+$ is 36.9% ± 4.9% with a background level of $0.2\pm 0.05({\rm syst})\pm$$0.2({\rm stat})$ events after 10 years of data collection. The estimated sensitivity based on 200 kton-years of exposure is $9.6 \times 10^{33}$ years, which is competitive with the current best limits on the proton lifetime in this channel and complements the use of different detection technologies