海上丝绸之路与闽南戏曲当代发展——东亚文化格局中的闽南戏曲二次创业研究

通过海上丝绸之路,闽南戏曲在历史上曾经成功地“走出去“与“走进去“,形成影响深远的“闽南戏曲文化圈“。闽南戏曲承载着闽南族群在地化、处身性的民间记忆,区别于在选择性过滤机制作用下之平板整一的宏大叙述,在全球本土化的时代语境中起着重述历史记忆与抵抗时间遗忘的重要作用。缘此,作为闽南族群重要的精神家园,闽南戏曲并非与现实无涉的休闲审美活动,其深刻表征海内外闽南人共有的情感结构与文化记忆,成为闽南族群建构身份认同的路径之一。国家社科基金项目《“陈三五娘”故事的传播及其当代意义研究》(11BZW107)阶段性成果; 国家级大学生创新创业训练计划《东亚文化语境中的闽南戏曲二次创业研究》(201410399009)研究成果; 泉州师范学院2014年度大学生科研基金项目《东亚文化格局中的泉州戏曲当代发展研究》(2014DSK14)最终成

Study on the Interaction of Gliotoxin with BSA

应用荧光、圆二色和紫外—可见吸收等波谱法研究胶毒素与牛血清白蛋白(bSA)的相互作用。荧光光谱实验结果表明胶毒素主要靠疏水作用与bSA结合,而对其内源荧光产生猝灭作用,其淬灭方式为静态猝灭,胶毒素与bSA的结合常数为7.2x103l/MOl。圆二色光谱检测发现,随着胶毒素浓度的增加,bSA的α-螺旋数量也增加,当胶毒素浓度为bSA浓度的100倍时,bSA的α-螺旋增加40.1%,表明胶毒素与bSA的结合改变了bSA的空间构象。The interaction between Gliotoxin and bovine serum albumin (BSA) was studied by the fluo-rescence, Circular Dichroism (CD) and ultraviolet visible (UV-Vis) techniques.The fluorescent experiment showed that the intrinsic fluorescence of BSA was quenched by the binding of gliotoxin in a static quenching procedure, with an association constant of 7.2×103 L/mol and in hydropobic forces.And the CD spectrum revealed that gliotoxin effected the conformation of BSA by increased the mass of α-helix.国家863计划项目(No.2006AA09Z410

A Rapiderodiagnosis For Antibodies Against Salmonella LPS

建立两种颗粒——乳胶颗粒及磁性颗粒快速检测沙门氏菌lPS相关抗体（IgM或Igg的方法。乳胶颗粒用lPS包被，磁性颗粒用羊抗人IgM或Igg包被，将两种颗粒及血清标本置于酶标条的微孔中，室温振荡30MIn后置于磁铁上使磁性颗粒沉淀。上清液在400nM的酶标仪上读取浊度以判定反应结果。该方法比乳胶凝集试验的灵敏度高。其结果与用ElISA方法检测的结果一致，但步骤少，时间短，操作更简便。是一种快速诊断沙门氏菌感染的有效方法。An eFFicient assay has been developed For detection antibodies againstSalmonella lipopolysaccharide(LPS).Latex coated with I/PS,polystyrene beads( Dynabeads )coated with goat anti-human μ or γ,and serum is mixed in the well of micro plate and shaken For30 min at room temperature。Then sediment by a magnet, the turbidity of the resuItant latexsuspension is measured spectrophotometrically at wavelength of 400 nm.The assay gives a highersensitivity as compared with latex agglutination.The results are in accordance with ELISA,while easier and can be accomplished by Fewer steps

Research on Effects of Gap on Weld Quality of Unequal-thickness Blanks Tailored Laser Welding and Its Compensation Method

焊缝凹陷是激光拼焊板的常见缺陷之一,严重影响焊缝强度,间隙是产生焊缝凹陷的主要原因。基于理论分析和工艺试验,研究了间隙的产生原因和对焊缝质量的影响,分析了间隙与工艺参数等因素的关系。研究结果表明:在不等厚板激光拼焊中,允许间隙的大小主要与偏移量、板厚组合、焊缝宽度以及薄板最小熔宽有关,薄板最小熔宽主要与钢板的材料有关。可以采用正偏移量对间隙进行补偿。该研究通过建立最大允许间隙和偏移量的计算公式,给出了间隙的偏移量补偿方法,从而提高了激光拼焊对间隙的适应能力。该结果可为优化工艺参数提供理论指导

RAPD Analysis of the Clustering Variation of Agarucus bisporus and Cloning of the Different DNA Fragment

通过双孢蘑菇正常菌株及其丛生变异菌株基因组DNA的RAPD研究,找到一个与双孢蘑菇丛生变异可能相关的DNA片段,将其克隆并测序.GenBank查询结果表明该片段可能的部分开放读框(ORF)与多种生物的二氢硫辛酰胺脱氢酶(DLDH)的部分氨基酸序列有较高的同源性,由此提出了一个双孢蘑菇丛生变异的分子机理假设.A DNA fragment possibly related to the clustering variation of A.bisporus was found by RAPD of the genomic DNA of healthy strains and their clustering variation ones, and it was cloned and sequenced. The results of GenBank searching suggested that the possible partial ORF of the fragment had a high homology with part of amino acids of dihydrolipoamide dehydrogenase (DLDH) from several organisms, so a molecular mechanism model for the clustering variation of A.bisporus was supposed.福建省自然科学基金(B0110032)资

Cloning of a DNA Fragment Correlated to the Quality of Agaricus bisporus Fruitbody

利用 2 0个十碱基随机引物 ,在基因组水平上对双孢蘑菇 (Agaricusbisporus)的优质低产型 (G型 )及低质高产型 (H型 )菌株进行了RAPD差异显示 ,获得一个与双孢蘑菇子实体品质相关的大小约为 2 0 0 0bp的差异DNA片段 ,将其克隆 ,并应用点杂交及RFLP技术对其区分两类菌株的有效性进行了检验。这为进一步建立特异性探针或PCR引物 ,从分子水平上提高双孢蘑菇菌种性状早期预测的准确性奠定了基础With 20 ten-mers random primers, the genomic differences between two types of Agaricus bisporus strain-good quality with low yield (G type) vs. poor quality with high yield (H type), were displayed by Random Amplified Polymorphic DNA (RAPD) technique. A 2000bp specific DNA fragment correlated to the quality of Agaricus bisporus fruitbody was obtained and cloned. Then dot hybridization and RFLP technique were used to determine the effectiveness of distinguishing two types of A. bisporus strains. And it was considered that our study had laid a foundation for further establishing a specific probe or PCR primer and for improving the accuracy of early predicting the characteristics of A. bisporus strains at molecular level.福建省自然科学基金资助项目!(C982 0 0 0 5

RAPD ANALYSIS OF TWO GENETIC FAMILIES OF AGARICUS BISPORUS

应用ＲＡＰＤ技术分析两个由同工酶为遗传标记建立的典型双孢蘑菇遗传家系中各亲子菌株间的遗传相关性，结果表明，单孢同校体的ＲＡＰＤ图谱较异核体而言有单一化的倾向；同时，随着遗传代数的增加，杂种子代与出发异核亲本间的遗传差异逐渐增大；研究结果还进一步印证同核单孢杂交比传统的单抱、多孢筛选更易产生优势明显的杂种．Using Random Amplified Polymorphic DNA (RAPD) techinque, the genetic relationship of the strains of two genetic families of barious bisporus, which had been founded based on the genetic marker of isozyme, was examined. The results showed that there was obvious decreasing tendency of DNA amplification polymorphism in homokaryons; as the number of genetic generation increases, the genetic distance between progenise and the original arents from which bybrids derived increased; hybrids had more advantageous characters than those isolates obtained by tranditional mule-spore or single-spore screening.福建省自然科学基金!No．C982000

Differential Proteomics Studies on Thermotolerance of Agaricus bisporus

通讯作者: [email protected][中文文摘]利用蛋白质组学和荧光定量PCR方法,研究双孢蘑菇(Agaricus bisporusLange(Imbach))02菌株在常温与高温胁迫下的蛋白质表达差异,发现了6个差异蛋白质点,经NCBI数据库比对分析,表明6个差异蛋白质分别为HSP70家族的HSS1热激蛋白、异柠檬酸裂解酶、细胞色素P450单氧化酶及3个未知蛋白质.这些蛋白质在双孢蘑菇受热胁迫下具有保护自身稳定的功能.[英文文摘]Agaricus bisporus is a kind of cultivated edible mushroom which has high economic value and important ecology significance.Environment temperature is the main restrained factor in its development,and this greatly limits its cultivation season.In this study,2D-eletrophoresis and real-time PCR are employed to discover the proteomic difference in Agaricus bisporus 02 strain after high temperature induction,and 6 differential expressed protein spots were finally obtained.Compared with the NCBI data base,spot 1 is the heat shock protein,spot 2 is isocitrately ase and spot 3 is cytochrome P450 monooxygenase.The other three spots are novel.Presumably, those proteins confer the thermo tolerance on strain 02 under high temperature.国家基础科学人才培养基金(J0630649);国家自然科学基金(30570254)资

MOLECULAR GENETIC STUDY ON THE PEDIGREE OF HYBRID STRAIN AS2796 OF AGARICUS BISPORUS

应用PCR和凝胶电泳等技术，对双孢蘑菇杂交菌株As2796及其亲本和子代作分子遗传标记跟踪分析，结果如下: 1) 总DNA的RAPD分析表明，随着遗传代数的增加，杂种子代和出发异核体亲本间的遗传差异逐渐增大; 2) mtDNA的酶切图谱表明，亲本8213及其杂交子代具有相同的基因型，表明双孢蘑菇的mtDNA呈单亲遗传; 3) Est同工酶的PAGE图谱表明， 结合了亲本02高产特征和8213优质特征的杂交子代具有两个亲本的标记带型，证明Est同工酶标记是双孢蘑菇新菌株特性预测或鉴定的有效指标。The pedigree of the hybrid A.bisporus strain As2796 was analyzed through molecular genetic markers tracking. The results were as follows: 1)RAPD analysis of total DNA showed that with the addition of the number of genetic generations， the genetic differences between hybrid offspring and their original heterokaryotic parents increased. 2)The patterns of mtDNA digested by endonucleases showed that the hybrid offspring had the same mitochondrial genotype as the parent 8213， indicating that the mtDNA of A.bisporus was inherited from one of the parents. 3)PAGE patterns of esterase isozyme(Est) showed that the hybrid offsprings which combined both the characteristic of high yield of parent 02 and good quality of 8213 had the same marked bands of their parents. The Est isozyme marker was found to be an effective index of new strains' characteristic prediction or determination of A.bisporus.福建省自然科学基金重点资助项目!（C9820005

Observation and Isolation of the Viruses in Cultured Penaeus Japonicus

从患病毒病的日本对虾剥取肝胰脏组织切片后电镜观察发现，很多肝胰细胞核中有典型的杆状病毒，病毒粒子已成熟，具有清晰可见的包膜结构，两端钝圆，直径约70~80nM，长度约250~300nM，有的整个细胞核中充满了未成熟的病毒粒子，核结构已瓦解，未观察到病毒包含体的存在，该病毒可能为bMnV.有的病虾标本肝胰细胞质中含有直径约55~60nM的具包膜结构的球状病毒.中肠中也发现了染色深的病毒颗粒，并有一些尚未成熟的病毒颗粒在微绒毛中形成串珠状结构，但均无发现病毒包含体.分别采用不同的提取方案从这些病虾中分离纯化了两种病毒，一为70~80nMx300~380nM的bMnV；另一为直径约60~65nM的日本对虾球状病毒，它们的大小与电镜测量值相一致Sections were made from hepatopancreas of penaeus japonicus affecting viral disease,and the typical and mature particales of baculoviruses had been observed in the hepatopancreatic cells by electron microscopic examination.The rodshaped virions were enveloped approximately with a diameter at 70～80 nm and length at 250￣300 nm, and identified as BMNV.There were unmature and mature viral particles in some cellular nucleus with collapsed structrue,but the inclusion body was not found.The enveloped spheroidviruses with 55～60 nm in diamater,which possibly belonged to RLV,were also observed in the cytoplasm of some hepatopancreatec cells.In the midgut of the shrimp,there existed stained viral particles and some unmature viral particles in microvilli but non inclusion body was observed in both cases.Two kinds of viruses,the baculovirus of 70～80 nm×300～380 nm(BMNV)and the spheroidvirus with a diamdter of 60～65 nm,were purified from P.japonicus with different methods.厦门市重大课题招标资