建立两种颗粒——乳胶颗粒及磁性颗粒快速检测沙门氏菌lPS相关抗体（IgM或Igg的方法。乳胶颗粒用lPS包被，磁性颗粒用羊抗人IgM或Igg包被，将两种颗粒及血清标本置于酶标条的微孔中，室温振荡30MIn后置于磁铁上使磁性颗粒沉淀。上清液在400nM的酶标仪上读取浊度以判定反应结果。该方法比乳胶凝集试验的灵敏度高。其结果与用ElISA方法检测的结果一致，但步骤少，时间短，操作更简便。是一种快速诊断沙门氏菌感染的有效方法。An eFFicient assay has been developed For detection antibodies againstSalmonella lipopolysaccharide(LPS).Latex coated with I/PS,polystyrene beads( Dynabeads )coated with goat anti-human μ or γ,and serum is mixed in the well of micro plate and shaken For30 min at room temperature。Then sediment by a magnet, the turbidity of the resuItant latexsuspension is measured spectrophotometrically at wavelength of 400 nm.The assay gives a highersensitivity as compared with latex agglutination.The results are in accordance with ELISA,while easier and can be accomplished by Fewer steps
