Determination of Trace Ammonia in Water by Fluorescence Spectrophotometry Combining with Flow-Injection Analysis

研究了在碱性介质中， 基于氨（ 铵离子） 与邻苯二甲醛及2 - 巯基乙醇反应生成强荧光性吲哚取代衍生物的体系测定微量氨（ 铵离子） 的流动注射荧光分析法. 非离子表面活性剂曲拉通X-100 （TrITOn X- 100） 对该体系具有良好的增稳作用. 研究了该体系测定氨（ 铵离子） 的最佳实验条件. 测定氨（ 铵离子） 的线性范围0 ~0-7Mg/l， 相对标准偏差为1-3 % （ CnH3 ＝ 0-2Mg/l，n ＝ 11） ，检测限为0-0012Mg/l.A method is described for determination of trace ammonia by fluorescence spectrophotometer combining with flow-injection Analysis.The method is based on the system:in base media,ammonia reacts with o-phthaladehyde(OPA) and 2-mercaptoethanol(ME) producing derivative of indol which has intensive fluorescence.Nonionic surfactant Triton X-100 enhances stability in the system.The optimum reaction condition is investigated.A linear relationship between ammonia concentration and fluorescent intensity is observed over the range 0 1～0 7mg/L with a relative standard deviation(RSD) of 1 3% at ammonia concentration of 0 2 mg/L(n=11).The limit of determination is 0 001 2mg/L.国家863计划资助!（818－Q－09

Research of Oxygen Fiber-Optic Sensor with Complexes of Ru(Ⅱ) as Probe

研究了PH、温度对钌（ Ⅱ） 的配合物ru （bPy）3 Cl2 及ru （PHEn）3 Cl2（bPy ＝ 2 ，2′- 联吡啶，PHEn ＝ 1 ， 10 - 邻菲罗啉） 荧光性质的影响， 并试验了O2 对ru （PHEn）3Cl2 在水溶液、醋酸纤维素膜及多孔塑料光纤中的荧光猝灭情况.Determination of trace O 2 based on O 2 quenchingfluorescence of complexes of Ru(Ⅱ) has been an advanced research region in fiber-optic chemical sensor for recent years.Effects of pH,temperature on fluorescence of Ru(bpy) 3 Cl 2 and Ru(phen) 3 Cl 2 are described in this article.And O 2 quenching fluorescence of Ru(phen) 3 Cl 2 in water,celluloseacetate film,plastic porous fiber-optic is investigated too.国家863计划资助!（818－Q－09

Ammonia-Sensitive Porous Plastic Fiber Optical Probe Utilising Tri-iodo-fluorescein as Fluorescence Indicator

研究了以甲基丙烯酸甲酯、双甲基丙烯酸四乙二醇酯和庚烷为聚合原料， 藻红为荧光指示剂对氨敏感的多孔塑料光纤探头， 同时探讨PH 及氨对其荧光强度的影响.Ammonia-sensitive porous plastic fiber optical probe, which is polymerized by methyl methylacrylate,tetraethylene-glycol bis(methylacrylate) and heptane,utilising tri-iodo-fluorescein as fluorescent indicator is invistigated;effect of pH and ammonia is also discussed.国家863计划资助!（818－Q－09

Determination of Trace PO_4--(-3) by Quenching Fluorescence with Flow Injection Analysis

研究了以铝- 桑色素为荧光试剂， 在酸性条件下利用荧光熄灭法， 并结合了流动注射分析测定了水中微量磷酸根离子的方法. 测定PO3 -4 的线性范围1 ~9 Mg/l， 检测限为0-002 Mg/l， 1H可以测定60 份样品， 相对标准偏差为1-09 % .A method is investigated to determinate trace PO 4 3- in acid solution based on oxygen quenching aluminium-morin fluorescence intensity combining with flow-injection analysis.The linearship is between 1～9mg/L with the limit of detection 0 002mg/L.60 samples per hour can determinated by this method with RDS 1 09%.国家863计划资助!（818－Q－09

Hollow Mesoporous Zirconia Nanocapsules for Drug Delivery

通讯作者地址: Tang, SH (通讯作者), Xiamen Univ, Coll Chem & Chem Engn, State Key Lab Phys Chem Solid Surfaces, Xiamen 361005, Peoples R China 地址: 1. Xiamen Univ, Coll Chem & Chem Engn, State Key Lab Phys Chem Solid Surfaces, Xiamen 361005, Peoples R China 2. Xiamen Univ, Coll Chem & Chem Engn, Dept Chem, Xiamen 361005, Peoples R China 电子邮件地址: [email protected] mesoporous zirconia nanocapsules (hm-ZrO2) with a hollow core/porous shell structure are demonstrated as effective vehicles for anti-cancer drug delivery. While the highly porous feature of the shell allows the drug, doxorubicin(DOX), to easily pass through between the inner void space and surrounding environment of the particles, the void space in the core endows the nanocapsules with high drug loading capacity. The larger the inner hollow diameter, the higher their DOX loading capacity. A loading of 102% related to the weight of hm-ZrO2 is achieved by the nanocapsules with an inner diameter of 385 nm. Due to their pH-dependent charge nature, hm-ZrO2 loaded DOX exhibit pH-dependent drug releasing kinetics. A lower pH offers a faster DOX release rate from hm-ZrO2. Such a property makes the loaded DOX easily release from the nanocapsules when up-taken by living cells. Although the flow cytometry reveals more uptake of hm-ZrO2 particles by normal cells, hm-ZrO2 loaded DOX release more drugs in cancer cells than in normal cells, leading to more cytotoxicity toward tumor cells and less cytotoxicity to healthy cells than free DOX.NSFC 20925103,20871100,20721001 Fok Ying Tung Education Foundation 121011 RFDP 200803841010 NSF of Fujian Province 2009J06005 Key Scientific Project of Fujian Province 2009HZ0002-

Superparamagnetic core-shell polymer particles for efficient purification of his-tagged proteins

地址: 1. Xiamen Univ, Coll Chem & Chem Engn, State Key Lab Phys Chem Solid Surfaces, Xiamen 361005, Peoples R China 2. Xiamen Univ, Coll Chem & Chem Engn, Dept Chem, Xiamen 361005, Peoples R China 电子邮件地址: [email protected] core-shell Fe3O4@SiO2@poly(styrene-alt-maleic anhydride) spheres enriched with Ni-NTA on their surface have been prepared by precipitation polymerization. The spheres have a core composed of superparamagnetic polycrystalline magnetite having a uniform size of similar to 220 nm, endowing the spheres with excellent magnetic responsivity and dispersity. The shell composition of poly(styrene-alt-maleic anhydride) allows the incorporation of more Ni-NTA affinity sites onto the surface of the magnetic spheres. Owing to the multivalency effect, the separation capacity of His-tagged proteins by the as-prepared Fe3O4@SiO2@polymer/Ni-NTA composites was four times as that by Fe3O4@SiO2/Ni-NTA, making them particularly promising for the magnetic separation of low-concentration His-tagged proteins. The magnentic polymer hybrid particles also exhibited excellent performance in the direct separation of His-tagged proteins from cells lysates.NSFC 20925103,20871100,20721001 Fok Ying Tung Education Foundation, 121011 MSTC,2009CB930703;NSF of Fujian Province 2009J06005; Key Scientific Project of Fujian Province 2009HZ0002-

灰绿曲霉产纤维素酶的研究

灰绿曲霉(Aspergillus glaucus)发酵液通过硫酸铵盐析、Sephadex G-100分子筛、DEAE Sepharose Fast Flow离子交换柱和Phenyl Sepharose Fast Flow疏水层析,分离纯化一种外切葡聚糖酶(CBH)和一种内切葡聚糖酶(EG).通过SDS-PAGE和凝胶柱层析法测定分子质量表明:CBH全酶分子质量为71 ku,由两个分子质量为35 ku的同型亚基组成;EG为单体蛋白,全酶分子质量为32 ku.酶学性质研究表明:CBH催化pNPC的最适pH为6.0,最适温度为55℃,酶活在pH 5.0~8.0区间和温度低于55℃时稳定;EG催化CMC-Na的最适pH为4.0,最适温度为50℃,酶活在pH3.5~7.5区间和温度低于65℃时稳定.Na+、K+、Ba2+、Mg2+以及NO3-和SO42-对CBH和EG酶活均无影响;Ca2+和Mn2+对CBH有激活作用,Fe2+和Mn2+对EG有激活作用,而Zn2+、Cd2+和Cu2+对CBH和EG均有不同程度的抑制效应.酶动力学分析表明:CBH催化pNPC水解的米氏常数Km值为1.4 mmol/L(pH 6.0,55℃),EG催化CMC-Na水解的米氏常数Km值为5.0 mg/mL(pH 4.0,50℃)

An iron silicate based pH-sensitive drug delivery system utilizing coordination bonding

E-mail Addresses: [email protected] we report a drug delivery system based on hollow iron silicate nanospheres. Fe3+ on the nanospheres' surface can effectively bind with doxorubicin (DOX), an anticancer drug, through coordination bonds. The bonds are fairly stable in a neutral environment but could easily break up in an acid environment. The release of DOX from hollow iron silicate nanospheres into cancer cells can be therefore triggered by a pH drop caused by endocytosis. The iron silicate shell allows a DOX loading content of up to 50.2% in weight, which is significantly higher than most drug delivery systems reported. Cell experiments show that DOX-loaded hollow iron silicate nanospheres exhibit a higher efficiency in killing cancer cells than free DOX, and a higher cytotoxicity for human hepatoma cells than hepatocyte cells at the same DOX-loaded nanospheres' concentration. Confocal laser scanning microscopy (CLSM) experiments show the releasing and transportation process of DOX, and confirm the enrichment of DOX in cell nuclei.MOST of China 2011CB932403 NSFC 21131005 21021061 20925103 Fok Ying Tung Education Foundation 12101

Preparation and photodynamic therapy application of NaYF4:Yb, Tm-NaYF4:Yb, Er multifunctional upconverting nanoparticles

E-mail Addresses: [email protected]; [email protected] preparation, characterization and application of NaYF4:Yb3+, Tm3+-NaYF4:Yb3+, Er3+ core-shell upconversion nanocrystals (UCNPs) with multiple emission peaks (e.g. 539, 654 and 802 nm) have been demonstrated in this work. The monodisperse nanocrystals were prepared via a modified thermal decomposition synthesis. The resulting UCNPs were similar to 31 nm in diameter with the lanthanide ions Tm3+ and Er3+ doped in the core and the shell, respectively. Under the laser diode excitation at 980 nm, these core-shell nanocrystals give strong upconversion emissions from the visible to near-infrared (NIR) region. By coating a PEG-phospholipid (PP) layer on the surface of the nanocrystals, the as-prepared UCNPs were favorably endowed with good water solubility for the potential biological applications. Here, a photosensitizer drug of Chlorin e6 (Ce6), which has maximum absorption that overlaps with the red emission of UCNPs, was loaded on these PP-coated UCNPs (UCNP@PP) by physical adsorption. The activity of the Ce6-loaded UCNP@PP (UCNP@PP-Ce6) in photodynamic therapy of cancer cells in vitro has been fully investigated in this work. Our results indicated that these multifunctional UCNP@PP-Ce6 nanoparticles have efficient NIR-to-NIR upconversion luminescence and photodynamic therapy capabilities, which could be potentially employed as a theranostic platform for cancer treatment.National Natural Science Foundation of China 21101131 21021061 20925103 Natural Science Foundation of Fujian Province 2012J01056 Fundamental Research Funds for the Central Universities 2010121015 Scientific Research Foundation for the Returned Overseas Chinese Scholars of State Education Ministry NFFTBS J121001

Core-Shell Pd@Au Nanoplates as Theranostic Agents for In-Vivo Photoacoustic Imaging, CT Imaging, and Photothermal Therapy

通讯作者地址: Liu, GMinistry of Science and Technology of China 2011CB932403 2014CB932004 National Natural Science Foundation of China 21420102001 21131005 8142202