Study on synthesis and application of 17β-Estradiol molecularly imprinted composite membrane

以多孔乙酸纤维膜为载体,17β-雌二醇为模板,采用紫外光引发制备了17β-雌二醇乙酸纤维分子印迹复合膜。试验对膜的选择吸附性和分离透过性进行了探讨。实验结果表明,所合成的乙酸纤维分子印迹复合膜对模板分子17β-雌二醇具有较好的选择吸附性,吸附量达10.2μMOl/g;17β-雌二醇/17α-炔雌醇的分离因子为4.7。膜透过实验表明,该分子印迹复合膜能将17β-雌二醇与17α-炔雌醇分离开,表明17α-炔雌醇作为结构类似物在复合膜上的结合牢固性不如模板分子。In this paper,the 17β-Estradiol molecularly imprinted composite membrane through UV initiation was prepared by using porous cellulose acetate membrane as support.The selective binding properties and separation capacity of the membranes were investigated.The results showed that the 17β-Estradiol molecularly imprinted composite membrane had high binding capacity for 17β-Estradiol,the binding amount Q for 17β-Estradiol was 10.2 μmol/g and separation factor was 4.7 for 17β-Estradiol/17α-ethinylestradiol.Permeation experiment results showed that the molecularly imprinted composite membrane could separate 17β-Estradiol from 17α-ethinylestradiol.The bonding force between 17α-ethinylestradiol and the membrane was weaker than the template.福建省教育厅B类项目(JB09241

A Study on Electrochemical Activity and ECL Properties of Some Bioactive Peptides

探讨了某些生物活性肽的电化学和电致化学发光特性的联系.The relativity of the peptides′ electrochemistry active and chemiluminescence intensity information are investigated.The electrochemiluminescence behavior of some bioactive peptides was studied by FIA-ECL.These peptides′ electrochemistry active at the glassy carbon electrode was also studied using a BAS-100 model electrochemical analyzer.国家自然科学基金!（296750003）;福建省自然科学基金!（B9810006

The Establishment of a FIA System with ECL Detection

讨论了一种流动注射电致化学发光检测系统的研制， 并用luMInOl 对其性能进行了测试.A novel ECL detection system for HPLC and FIA has been set-up.The performance of our detection system has been studied with luminol.国家自然科学基金!（296750003）;福建省自然科学基金!（B9810006

Determination of Glutathione by FIA with ECL Detection

发现氧化型谷胱甘肽能增强钌联吡啶的电致化学发光， 而还原型谷胱甘肽抑制钌联吡啶的电致化学发光. 根据增强或抑制程度的大小， 可定量测定谷胱甘肽.We reported the determination of glutathione based on electrogenerated chemiluminescence in the presence of ruthenium compound.The various parameter influencing the ECL-FIA of peptides as electroysis pulse parameters and medium conditions were optimized indetail.The sensitivity is sufficient.国家自然科学基金!（296750003）;福建省自然科学基金!（B9810006

表层弥散预应变硬化对薄钢板变形局部化的影响

试验研究了具有一定形貌及粗糙度的激光毛面薄钢板及同材质普通薄钢板的失稳行为。结果表明激光毛面薄钢板变形局部化过程明显滞后于普通板，其表层弥散分布的预应变硬化点在稳定薄板变形、延缓变形局部化过程中起到重要作用

激光和电子束表面强化技术的发展及其应用

激光和电子束表面强化是当前引入注目的两种高新技术。文章系统地介绍了这两种技术的发展状况及应用，同时比较了二者的优缺点。最后对目前存在的问题及未来的发展方向作了简要的讨论

Determination of Trace Ammonia in Water by Fluorescence Spectrophotometry Combining with Flow-Injection Analysis

研究了在碱性介质中， 基于氨（ 铵离子） 与邻苯二甲醛及2 - 巯基乙醇反应生成强荧光性吲哚取代衍生物的体系测定微量氨（ 铵离子） 的流动注射荧光分析法. 非离子表面活性剂曲拉通X-100 （TrITOn X- 100） 对该体系具有良好的增稳作用. 研究了该体系测定氨（ 铵离子） 的最佳实验条件. 测定氨（ 铵离子） 的线性范围0 ~0-7Mg/l， 相对标准偏差为1-3 % （ CnH3 ＝ 0-2Mg/l，n ＝ 11） ，检测限为0-0012Mg/l.A method is described for determination of trace ammonia by fluorescence spectrophotometer combining with flow-injection Analysis.The method is based on the system:in base media,ammonia reacts with o-phthaladehyde(OPA) and 2-mercaptoethanol(ME) producing derivative of indol which has intensive fluorescence.Nonionic surfactant Triton X-100 enhances stability in the system.The optimum reaction condition is investigated.A linear relationship between ammonia concentration and fluorescent intensity is observed over the range 0 1～0 7mg/L with a relative standard deviation(RSD) of 1 3% at ammonia concentration of 0 2 mg/L(n=11).The limit of determination is 0 001 2mg/L.国家863计划资助!（818－Q－09

关节镜下多股半腱肌腱与股薄肌腱重建前十字韧带的临床应用

目的探讨应用自体半腱肌腱与股薄肌腱重建前十字韧带(anteriorcruciateligament,ACL)的方法与疗效。方法对30例ACL损伤患者行关节镜下半腱肌腱与股薄肌腱ACL重建术。半腱肌腱与股薄肌腱均取自同侧,其中25例应用半腱肌腱与股薄肌腱进行重建;5例单纯应用半腱肌腱。将肌腱反复折叠为长约8.5cm的4～5股,在距两端2.5cm处分别用丝线编织捆绑缝合,用直径为7～8mm,长20～25mm的SoftSilk型挤压螺钉于屈膝20°～40°位固定,等长重建ACL。合并损伤于先期或同期治疗。结果术后随访7～30个月,平均15.9个月。所有患者术后4周膝关节均达正常活动范围。X线片显示24例内固定物位置良好,5例股骨侧和1例胫骨侧位置明显偏前。12例胫骨隧道和8例股骨隧道轻度扩大,无骨隧道明显扩大的病例。26例术后Lachman试验≤Ⅰ度,27例轴移试验阴性或Ⅰ度,Lysholm评分平均为87.5分,较术前平均54.5分显著增高,差异有显著性意义(P<0.05)。Tegner运动评级平均为5.2级,较术前平均2.8级有所增高。结论关节镜下挤压螺钉固定自体多股半腱肌腱与股薄肌腱重建ACL是治疗急、慢性ACL损伤的较好的方法

Research of Oxygen Fiber-Optic Sensor with Complexes of Ru(Ⅱ) as Probe

研究了PH、温度对钌（ Ⅱ） 的配合物ru （bPy）3 Cl2 及ru （PHEn）3 Cl2（bPy ＝ 2 ，2′- 联吡啶，PHEn ＝ 1 ， 10 - 邻菲罗啉） 荧光性质的影响， 并试验了O2 对ru （PHEn）3Cl2 在水溶液、醋酸纤维素膜及多孔塑料光纤中的荧光猝灭情况.Determination of trace O 2 based on O 2 quenchingfluorescence of complexes of Ru(Ⅱ) has been an advanced research region in fiber-optic chemical sensor for recent years.Effects of pH,temperature on fluorescence of Ru(bpy) 3 Cl 2 and Ru(phen) 3 Cl 2 are described in this article.And O 2 quenching fluorescence of Ru(phen) 3 Cl 2 in water,celluloseacetate film,plastic porous fiber-optic is investigated too.国家863计划资助!（818－Q－09