Effect and mechanism of three kinds of drinking water on blood pressure of hypertensive mice

目的探究3种饮用水对高血压小鼠血压的影响及可能机制。方法测定3种饮用水的水质情况:溶解性总固体(TDS) 、耗氧量(OC); 、pH、氧化还原电位(ORP) 、电导率(EC); 、溶解氢、钙和镁;采用N'-硝基-L-精氨酸甲酯盐酸盐(L-NAME)连续灌胃法(30天)建立ICR小鼠高血压模型。然后将小鼠随机分为4组:正常; 对照组、模型对照组、模型自来水组和模型过滤水组,每个月检测小鼠血压,3个月后测定小鼠血清一氧化氮(NO) 、一氧化氮合酶(NOS); 、总超氧化物歧化酶(T-SOD) 、丙二醛(MDA) 、谷胱甘肽过氧化物酶(GSH-Px) 、内皮素(ET) 、血管紧张素(Ang); 、醛固酮(ALD) 、儿茶酚胺(CA) 、白细胞介素-6(IL-6); 、超敏反应蛋白(hs-CRP)及环磷酸鸟苷(c-GMP)含量;取小鼠心脏、肾脏和胸主动脉组织制作石蜡切片观察其形态。结果过滤水pH、TDS、OC; 、EC、钙和镁均高于纯水和自来水,ORP呈负值,且溶解氢含量高;模型过滤水组小鼠收缩压与平均血压[(106. 24 5. 31 )和(90.; 73 4. 99 ) mmHg]低于模型对照组[(119. 58 6. 08)和(96. 44 6. 48); mmHg],差异有统计学意义(P < 0. 05);模型过滤水组NO含量[(87. 05 39. 82) mumol; /L]明显高于模型对照组[(45. 01 9. 62) mumol /L]和模型自来水组[(46. 56 30. 54) mumol; /L],差异有统计学意义(P < 0. 05);模型自来水组和模型过滤水组IL-6[(201. 42 36. 41)和(173. 99 ; 114. 96) pg /mL]和Ang含量[(1319. 20 111. 90)和(1349. 38 180. 15) ng; /L]显著低于模型对照组[(363. 14 149. 00) pg /mL和(1736. 17 242. 86) ng; /L],差异有统计学意义(P < 0. 05);模型过滤水组T-SOD和GSH-Px活性[(268. 37 12. 25)和(712. 45 ; 30. 59 ) U/mL]显著高于模型对照组[(250. 46 15. 60 )和(678. 36 35. 80); U/mL],差异均有统计学意义(P < 0. 05) 。结论与纯水、自来水相比,过滤水可通过抗氧化、抗炎作用降低高血压小鼠血压。Objective To explore the effect of three kinds of drinking water and its; possible mechanism on blood pressure of hypertensive mice. Methods The; water quality parameters were measured for three kinds of drinking; water,and the parameters included total dissolved solids(TDS) ,oxygen; consumed(OC) ,pH,oxidation reduction potential(ORP ) ,electric; conductivity(EC ) ,dissolved hydrogen,calcium and magnesium.; Establishment of mouse model of high blood pressure by using; N'-nitro-L-arginine methyl ester hydrochloride(L-NAME) for 30 days. Then; the mice were randomly divided into 4 groups: normal control group,model; control group,group of tap water and group of filtered water. Blood; pressure in mice was detected every month. After 3 months,nitric; oxide(NO ) ,nitric oxide synthase(NOS ) ,total superoxide; dismutase(T-SOD ) ,malondialdehyde(MDA ) ,glutathione peroxidase(GSH-Px; ) ,endothelin(ET ) ,angiotensin(Ang) ,aldosterone(ALD); ,catecholamine(CA) ,high-sensitivity C-reactive protein(hs-CRP) ,; interleukin 6(IL-6) and cyclic guanosine monophosphate(c-GMP) in serum; of the mice were determined. Simultaneously morphological changes of; heart,kidney and thoracic aorta paraffin section were observed. Results; The pH,TDS,OC,EC,calcium and magnesium in filtered water were higher; than that of pure and tap water. The negative ORP and great quantities; of dissolved hydrogen were found in filtered water; The systolic blood; pressure and mean blood pressure((106. 24 5. 31) and(90. 73 4. 99); mmHg) of filtered water were lower than those of the pure water(119. 58 ; 6. 08 and 96. 44 6. 48 mmHg)(P < 0. 05) . The NO((87. 05 39. 82) mumol; /L) in the mice with filtered water were higher than those of with pure; and tap water((45. 01 9. 62) and(46. 56 30. 54) mumol /L)(P < 0. 05) .; Compared with those of the mice with pure water, significant decrease in; IL-6((201. 42 36. 41) and(173. 99 114. 96) vs.(363. 14 149. 00) pg; /mL) and Ang((1319. 20 111. 90 ) and(1349. 38 180. 15 ) vs.(1736. 17 ; 242. 86) ng /L) were observed in mice treated with tap and filtered; water(P < 0. 05) . The T-SOD and GSH-Px((268. 37 12. 25) and(712. 45 ; 30. 59) U/mL) in the mice with filtered water were significant higher; than those of pure water((250. 46 15. 60) and(678. 36 35. 80 ) U/mL)(P; < 0. 05 ) . Conclusion Compared with pure and tap water,filtered water; can reduce the blood pressure of the hypertensive mice by antioxidation; and anti-inflammatory.福建省高校产学合作项

抗SARS-CoV N蛋白单克隆抗体的特异性及其识别抗原表位的初步鉴定

为了明确抗SARS-CoVN蛋白单克隆抗体的特异性,并鉴定其识别表位,首先在E.coli中表达了人类冠状病毒229E(HCoV-229E)和OC43(HCoV-OC4)N蛋白,用Westernblotting和间接免疫荧光方法分别检测了4株抗SARS-CoVN蛋白单克隆抗体(1-1C2、1-1D6、2-8F11和2-2E5)与HCoV-OC43和HCoV-229E及其N蛋白的交叉反应情况,而后应用12种重组截短型SARS-CoVN蛋白对上述4种单克隆抗体的识别表位进行了初步定位。结果显示:(1)在4株抗N蛋白单克隆抗体中,1-1C2、1-1D6和2-2E5不与HCoV-OC43和HCoV-229E及其N蛋白发生交叉反应,为SARS-CoVN蛋白特异性抗体;(2)2-8F11、1-1D6和2-2E5针对的抗原表位位于SARS-CoVN蛋白的aa30-60,1-1C2针对的抗原表位则位于SARS-CoVN蛋白的aa170-184。这一研究为阐明SARS-CoVN蛋白的免疫学特征,建立特异性免疫诊断技术和研究其致病机制提供了必要的依据和材料

JUNO Sensitivity on Proton Decay p→νˉK+p\to \bar\nu K^+ Searches

The Jiangmen Underground Neutrino Observatory (JUNO) is a large liquid scintillator detector designed to explore many topics in fundamental physics. In this paper, the potential on searching for proton decay in $p\to \bar\nu K^+$ mode with JUNO is investigated.The kaon and its decay particles feature a clear three-fold coincidence signature that results in a high efficiency for identification. Moreover, the excellent energy resolution of JUNO permits to suppress the sizable background caused by other delayed signals. Based on these advantages, the detection efficiency for the proton decay via $p\to \bar\nu K^+$ is 36.9% with a background level of 0.2 events after 10 years of data taking. The estimated sensitivity based on 200 kton-years exposure is $9.6 \times 10^{33}$ years, competitive with the current best limits on the proton lifetime in this channel

JUNO sensitivity on proton decay p → ν K + searches*

The Jiangmen Underground Neutrino Observatory (JUNO) is a large liquid scintillator detector designed to explore many topics in fundamental physics. In this study, the potential of searching for proton decay in the $p\to \bar{\nu} K^+$ mode with JUNO is investigated. The kaon and its decay particles feature a clear three-fold coincidence signature that results in a high efficiency for identification. Moreover, the excellent energy resolution of JUNO permits suppression of the sizable background caused by other delayed signals. Based on these advantages, the detection efficiency for the proton decay via $p\to \bar{\nu} K^+$ is 36.9% ± 4.9% with a background level of $0.2\pm 0.05({\rm syst})\pm$$0.2({\rm stat})$ events after 10 years of data collection. The estimated sensitivity based on 200 kton-years of exposure is $9.6 \times 10^{33}$ years, which is competitive with the current best limits on the proton lifetime in this channel and complements the use of different detection technologies