Optimization of solid culture conditions for TNF-α inhibitor qiaonanmycin via response surface methodology

目的利用响应面法对潜在的Tnf-α抑制剂桥南霉素固态发酵培养基进行优化。方法用HPlC做检测方法,以桥南霉素的洗脱峰面积为指标,先通过PlACkETT-burMAn(P-b)实验筛选出影响固态发酵产量的主要因素,进而进行单因素优化实验和响应面中心组合设计实验,确定主要影响因素的最佳浓度,得到最优的固体发酵培养基组成。结果优化后的发酵培养基组成:土豆400g/l、葡萄糖35g/l、蛋白胨10.5g/l、(nH4)2SO4 0.6g/l。按此配方固态发酵10l,对得到的粗提物进行HPlC检测,桥南霉素的实际产量为4.3Mg/l,与模型的理论值基本相符,较优化前的产量1.5Mg/l提高了186.7%。结论响应面方法是一种有效的培养基优化方法,经此优化方法桥南霉素的产量得到了较大的提高。Objective The solid fermentation culture conditions of compound qiaonanmycin were optimized via response surface methodology.Methods Based on quantitative detection method of qiaonanmycin by HPLC, the key fermentation factors were determined via P-B test, then their optimized concentrations were confirmed through response face method.Results The optimum solid fermentation culture medium compositions of qiaonanmycin were: potato 400g/L, dextrose 35g/L, peptone 10.5g/L, and(NH4)2SO4 0.6g/L.Under this condition, the yield of qiaonanmycin was 4.3mg/L, resulting in a 186.7% increase comparing with original production yield, and the optimized yield was consistent well with theoretical value predicted by response face method.Conclusion The optimized solid fermentation culture conditions were reliable and repeatable, it was warranted for large-scale fermentation process.中央高校基本业务费(No.2010121092); 厦门市科技计划项目(No.3502Z20123010

Experiment on EHD Printing under DC High Voltage

基于直流电压作用下的电液耦合微喷印系统,考察了溶液浓度和电压两个参数对喷印过程和沉积形态的影响。实验结果表明射流在收集板上沉积的宽度随溶液浓度增大而减小;浓度低时射流沉积为连续形态;浓度增大,射流沉积为液滴-细线相间或者液滴-卫星液滴相间的珠状结构;浓度增大至18%时,产生纳米纤维,直径600nM左右。对给定的EHd实验系统,仅当电压值在2.6kV~4.8kV范围内变化时可保证稳定喷射;且随着电压值增大,珠状结构的直径减小;电压值在该范围之外,无法实现稳定喷射。Based on the EHD printing system under the DC high voltage,the influence of the concentration of solution and the voltage value on jetting process and the collected lines is investigated.Experimental results show that the collected line gets thinner with the concentration of solution increasing.The collected line is continuous at low concentration of solution.The bead form of a droplet with a filament or satellite droplets is collected with the concentration of solution increasing.At 18 % concentration of solution nanofibre with the diameter of 600nm is collected.For a given EHD printing system,when the voltage value is in the range of 2.6 kV ～ 4.8 kV,the jetting process is stable and the diameter of the bead decreases with the voltage value increasing.Outside this range,the stable jetting process can not happen.高等学校科技创新工程重大项目培育资金项目资助(708055);国家863项目资助(2007AA04Z308);国家自然科学基金项目资助(50675184

Time-resolved PL Spectra Study of Ordered Ga_(0.52)In_(0.48)P Alloys

【中文摘要】 在室温和低温液氮下 ,研究了有序和无序 Ga0 .52 In0 .4 8P的时间分辨发光谱。对实验结果的拟合表明 ,有序 Ga0 .52 In0 .4 8P的发光呈双指数规律衰退。其中快过程对应着有序区域上载流子的复合 ,慢过程则对应着有序区域和无序区域的空间分离中心上载流子的复合。无序 Ga0 .52 In0 .4 8P的发光在室温下呈单指数规律衰退。同时从低温下的时间分辨发光谱还可以看出有序样品的发光峰随着延迟时间的变长而蓝移 ,说明低温下在有序 Ga0 .52In0 .4 8P中存在着载流子从无序区域到有序区域的转移 【英文摘要】 The time resolved photoluminescence(TRPL) spectra of ordered and disordered Ga 0.52 In 0.48 P alloys grown by MOVPE were investigated.The least square fitting of the luminescence decay spectra of ordered alloys shows that there exist two exponential processes:The short lifetime process was corresponding to the high energy peak in PL spectra which is attributed to the recombination of the carriers in the ordered domains and the long lifetime process was corresponding to the low energy peak in the P...国家自然科学基金 (编号:69776011); 福建省自然科学基金 (编号:A9910004和 A0110007

新型稠环电子受体的界面修饰对钙钛矿太阳能电池性能的影响

钙钛矿太阳能电池(perovskite solar cells, PSCs)因具有能量转换效率(power conversion efficiency, PCE)高、成本低、易于大面积制造等优点而被科学家们广泛关注.氧化物电子传输层的合理界面设计及修饰对提高器件的PCE和工作长期稳定性有着十分重要的意义.因此,本文采用一种含有烷基噻吩基侧链的稠环电子受体材料3,9-二(2-亚甲基-(3-(1,1-二氰甲烯基)-茚酮))-5,5,11,11-四(5-己基噻吩)-二噻吩并[2,3-d:2′,3′-d′]-s-引达省[1,2-b:5,6-b′]二噻吩(3,9-bis(2-methylene-(3-(1,1-dicyanomethylene)-indanone))-5,5,11,11-tetrakis(5-hexylthienyl)-dithieno[2,3-d:2′,3′-d′]-s-indaceno[1,2-b:5,6-b′]dithiophene, ITIC-Th)修饰TiO2电子传输层,制备高效稳定的平面结PSCs.研究结果显示,ITIC-Th的界面修饰改善了TiO2薄膜的形貌、接触角等性质,促进了钙钛矿晶粒的高质量生长,大幅度减少了器件表界面的电荷复合,明显提升了光生载流子的抽取率和输运效率,使经ITIC-Th界面修饰的PSC的PCE从未经界面修饰的15.43%显著提高到18.91%.与此同时,器件稳定性的研究结果显示,在室温和湿度为30%的条件下,经ITICTh界面修饰的PSC的PCE在放置约1000 h后依然保持原来的90%,明显高于未经界面修饰的PSC.研究结果对PSC光伏性能的提升具有重要的实际应用价值和学术意义.国家自然科学基金(61605164);;\n陕西省重点科技创新团队计划(2016KCT-28);;\n陕西省重点研发计划(2017ZDXM-GY-046);;\n福建省科技厅高校产学合作项目(2016H6023)资

近海生态系统碳汇过程、调控机制及增汇模式

海洋是地球上最大的碳库,发挥着全球气候变化缓冲器的作用.蓝色碳汇,简称蓝碳,即由海洋生态系统捕获的碳(主要是有机碳),是海洋储碳的重要机制之一.; 蓝碳最初认识的形式是可见的海岸带植物固碳.其实之前没有得到足够重视的、看不见的微型生物(浮游植物、细菌、古菌、病毒、原生动物)占海洋生物量90%; 以上,是蓝碳的主要贡献者.中国陆架边缘海占国土总面积的1/3,碳汇潜力巨大,亟待研发.本文以近海生态系统碳汇过程、调控机制及增汇模式为主线,论述; 了近海生态系统结构与碳循环功能特征、碳汇形成过程与机理,并结合近海碳汇在沉积记录中的地史过程演变探讨了自然过程和人类活动对碳汇的可能影响,展望了; 碳汇工程在增加近海海洋储碳能力方面的应用前景.国家重大科学研究计划项目; 国家重点研发计划项目; 国家自然科学基金项目; 国家海洋局全球变化与海气相互作用专项项

Synthesis of C-3v-(C64H4)-C-#1911 using a low-pressure benzene/oxygen diffusion flame: Another pathway toward non-IPR fullerenes

通讯作者地址: Huang, RB (通讯作者), Xiamen Univ, Dept Chem, Coll Chem & Chem Engn, 422 S Shimin Rd, Xiamen 361005, Peoples R China 地址: 1. Xiamen Univ, Dept Chem, Coll Chem & Chem Engn, Xiamen 361005, Peoples R China 2. Xiamen Univ, State Key Lab Phys Chem Solid Surfaces, Xiamen 361005, Peoples R China 电子邮件地址: [email protected] addition to the traditional Kratschmer-Huffman graphite arc-discharge method, a combustion method has been developed to synthesize fullerenes that defy the isolated pentagon rule (IPR). The C-3v-symmetric (C64H4)-C-#1911, as well as other non-IPR fullerenes such as the possible non-IPR C-60 isomer, have been synthesized as fullerene hydrides in a low-pressure benzene/oxygen diffusion flame. The non-IPR structure of the chromatographically isolated C-64 species was unambiguously identified by its HPLC retention time, UV absorption and mass spectrum by comparison with the reference material C3v-#1911C64H4. The synthesis, isolation and identification of C-3v-(#1911) C64H4, as described in this work, show that this combustion method is successful, is energy efficient and is a promising candidate for the macroscopic synthesis of non-IPR fullerenes. (C) 2009 The Combustion Institute. Published by Elsevier Inc. All rights reserved.Institute of Chemistry, Chinese Academy of Sciences 20571062 20721001 20525103 National Basic Research Program (973 Program) of China 2007CB81530

The graphite arc-discharge in the presence of CCl4: Chlorinated carbon clusters in relation with fullerenes formation

通讯作者地址: Xie, SY (通讯作者), Xiamen Univ, Dept Chem, State Key Lab Phys Chem Solid Surfaces, Xiamen 361005, Peoples R China 地址: 1. Xiamen Univ, Dept Chem, State Key Lab Phys Chem Solid Surfaces, Xiamen 361005, Peoples R China 2. Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R ChinaThe graphite arc-discharge in the atmosphere consisting of CCl4 40 Torr and increasing partial pressure of He from 0 to 300 Torr produced, in addition to carbon species such as graphite and fullerenes C60 and C70, numerous chlorinated carbon clusters (CCCs). The yields of some CCCs, including C6Cl6 (perchlorobenzene), C10Cl8 (perchloronaphthalene), C12Cl8 (perchloroacenaphthylene) and C12Cl8 (isomer II), C14Cl8 (isomer I), C16Cl10 (perchlorofluoranthene), C18Cl10 (isomer I and II), and C20Cl10 (isomer I), correspond well with C60 yields, which may imply these CCCs share the same growth mechanism with fullerenes. Such a yield correlation can be used to distinguish the fullerene precursors from the irrelevant by-products, thus contributes to the mechanistic study of fullerene formation

Simple Combustion Production and Characterization of Octahydro[60]fullerene with a Non-IPR C-60 Cage

1. Xiamen Univ, State Key Lab Phys Chem Solid Surface, Xiamen 361005, Peoples R China 2. Xiamen Univ, Dept Chem, Coll Chem & Chem Engn, Xiamen 361005, Peoples R China 3. Xiamen Univ, Sch Life Sci, Xiamen 361005, Peoples R ChinaFor the first time an easier, operable combustion method is employed for the synthesis of non-IPR fullerene, and an octahydro[60)fullerene with a non-IPA C-60 cage (C-60 isomer C-#1809(60)) produced by combustion is isolated and characterized by MS, UV vis, IR, and NMR spectroscopies in combination with DFT calculations. This finding shows that, in addition to chlorine, hydrogen can be an ample cataloreactant for the production of non-IPR fullerene derivatives under such conditions as arc-burning and diffusion combustion.NSFC 20525103 20673088 20973137 20721001 20423002 21031004 973 projects 2007CB815301 2007CB81530

Combustion Synthesis and Electrochemical Properties of the Small Hydrofullerene C50H10

通讯作者地址: Xie, SY (通讯作者),Xiamen Univ, Coll Chem & Chem Engn, State Key Lab Phys Chem Solid Surfaces, Xiamen 361005, Peoples R China 地址: 1. Xiamen Univ, Coll Chem & Chem Engn, State Key Lab Phys Chem Solid Surfaces, Xiamen 361005, Peoples R China 2. Xiamen Univ, Coll Chem & Chem Engn, Dept Chem, Xiamen 361005, Peoples R China 3. Xiamen Univ, Sch Life Sci, Xiamen 361005, Peoples R China 电子邮件地址: [email protected]; [email protected] hydrofullerene C50H10 is synthesized by low-pressure benzeneoxygen diffusion combustion. The structure of C50H10 is identified through NMR, mass spectrometry, and IR and Raman spectroscopy as a D5h symmetric closed-cage molecule with five pairs of fused pentagons stabilized by ten hydrogen atoms. UV/Vis and fluorescence spectrometric analyses disclose its optical properties as comparable with those of its chloride cousin (C50Cl10). Cyclic and square-wave voltammograms reveal that the first reduction potential of C50H10 is more negative than that of C50Cl10 as well as C60, with implications for the utilization of C50H10 as a promising electron acceptor for photovoltaic applications.973 projects 2011CB935901 NSFC 21031004 21021061 2077310

1978～2008年中国湿地类型变化

分别基于美国陆地卫星(Landsat MSS/TM/ETM+)和中巴资源卫星(CBERS-02B)影像数据,以人工目视解译为主,完成了中国1978～2008年4期(基准年分别为1978,1990,2000和2008年)湿地遥感制图,并进行了大量的室内外验证.在此基础上,对我国湿地现状及近30年来湿地变化进行了初步分析,得到以下主要结论:(ⅰ)截止2008年,中国湿地面积约为324097km2,其中以内陆沼泽(35%)和湖泊湿地(26%)为主.(ⅱ)1978～2008年,中国湿地面积减少了约33%,而人工湿地增加了约122%.过去30年里湿地减少的速度大幅降低,由最初5523km2/a(1978～1990年)降为831km2/a(2000～2008年).(ⅲ)减少的自然湿地(包括滨海湿地和内陆湿地),其类型变化由湿地向非湿地转化的比例逐渐降低.初期(1978～1990年)几乎全部(98%)转换为非湿地;在1990～2000年间减少的自然湿地约有86%转化为非湿地,而在2000～2008年,这一比例下降为77%.(ⅳ)气候变化和农业活动是中国湿地变化的主要驱动因素,湿地变化在中国分为三大不同特征区域,即西部三省/自治区(西藏、新疆和青海)、北部两省/自治区(黑龙江和内蒙古)和其他省市区.其中西部区域尤其是青藏高原,湿地变化的驱动因子以气候增温为主;新疆湿地由于气候增温和农业活动共同作用造成变化不大.北部省/自治区的湿地变化则主要由农业活动引起;而其他省市区的湿地变化几乎完全受控于人类的农业经济活动