目的利用响应面法对潜在的Tnf-α抑制剂桥南霉素固态发酵培养基进行优化。方法用HPlC做检测方法,以桥南霉素的洗脱峰面积为指标,先通过PlACkETT-burMAn(P-b)实验筛选出影响固态发酵产量的主要因素,进而进行单因素优化实验和响应面中心组合设计实验,确定主要影响因素的最佳浓度,得到最优的固体发酵培养基组成。结果优化后的发酵培养基组成:土豆400g/l、葡萄糖35g/l、蛋白胨10.5g/l、(nH4)2SO4 0.6g/l。按此配方固态发酵10l,对得到的粗提物进行HPlC检测,桥南霉素的实际产量为4.3Mg/l,与模型的理论值基本相符,较优化前的产量1.5Mg/l提高了186.7%。结论响应面方法是一种有效的培养基优化方法,经此优化方法桥南霉素的产量得到了较大的提高。Objective The solid fermentation culture conditions of compound qiaonanmycin were optimized via response surface methodology.Methods Based on quantitative detection method of qiaonanmycin by HPLC, the key fermentation factors were determined via P-B test, then their optimized concentrations were confirmed through response face method.Results The optimum solid fermentation culture medium compositions of qiaonanmycin were: potato 400g/L, dextrose 35g/L, peptone 10.5g/L, and(NH4)2SO4 0.6g/L.Under this condition, the yield of qiaonanmycin was 4.3mg/L, resulting in a 186.7% increase comparing with original production yield, and the optimized yield was consistent well with theoretical value predicted by response face method.Conclusion The optimized solid fermentation culture conditions were reliable and repeatable, it was warranted for large-scale fermentation process.中央高校基本业务费(No.2010121092); 厦门市科技计划项目(No.3502Z20123010
