The Exploration of the Locale Network Construction based on Strategic Transition of China Telecom

中文摘要 当前，中国电信的企业战略转型已经进入新的阶段。集团公司2008年初提出：今后三年要“实施聚焦客户的信息化创新战略，努力成为业界领先的综合信息服务提供商”。本文以中国电信W市分公司为背景应用麦肯锡业务“三层面理论”探索和研究本地网[1]在市场竞争日趋激烈，固网话音业务不断下滑的大环境下，如何实现集团公司战略转型尤其是核心的业务转型在本地网落地和执行。我们针对工作中所遇到的问题进行调查研究，认识到当前重要的是要做好集团战略转型的战术组织和业务转型的有效实施提高团队执行力，要注重基础和转型业务组合营销，确保企业持续发展。最后还对全业务经营后的转型之路提出了自己的观点。 文章坚持以市场为...Abstract At present, China Telecom's business strategy in transition has entered a new stage. Group early in 2008 has announced: the next three years to "focus the implementation of information technology, innovative strategies, and strive to become the industry's leading integrated information service providers." Based on McKinsey’s “Three Dimensions Theory”, this paper has China Telecom W City ...学位：管理学硕士院系专业：管理学院高级经理教育中心（EMBA项目）_高级管理人员工商管理硕士(EMBA)学号：X200715602

Leukemia fusion genes and gene mutation detection methods based on real-time quantitative PCR platform

白血病是造血系统的恶性疾病，随着科学技术的发展，白血病的研究也由形态学步入到分子生物学。研究发现白血病的发生是多因素、多基因、多阶段的复杂的生物学现象。引起白血病最为常见的原因是染色体异常（通常形成融合基因）及基因突变，其中很多融合基因或者基因突变可作为分子标记物，用于预后判断、治疗方案的选择、微小残留病的监控及靶向治疗。本论文基于实时荧光定量PCR平台，建立了两种方法，一种用于检测融合基因，一种用于检测基因突变，以期可以用于临床辅助治疗或诊断。 第一章绪论 本章回顾了白血病发现及研究历史，介绍了白血病的分子遗传学基础及各种分型诊断方法。除此之外对实时荧光定量PCR也做了概述，基于实时荧光...Leukemia is a malignant disease of the hematopoietic system. With the scientific and technological development, the research of leukemia enters into the molecular biology from morphology. Studies find that the occurrence of leukemia is a multi-factor,multi-gene, multi-stage complex biological phenomena. Chromosomal abnormalities (usually formation of fusion genes) and gene mutations are common in ...学位：理学硕士院系专业：生物医学研究院_化学生物学专业学号：3052009115224

ADCP检定水池物理尺寸探讨

为满足当前ADCP计量检定的迫切需求,根据ADCP在水池拖车试验检定时,换能器发射声波的主瓣、旁瓣与检定水池池壁、池底形成的几何关系,推导出了抑制旁瓣、不抑制旁瓣时检定水池的尺寸需求。以市场占有率最高的ADCP产品为研究对象,计算得出了不同工作频率ADCP对检定水池宽度和深度的理论值。导出的公式,进一步完善了ADCP计量检定的理论基础,为ADCP在室内可控环境下的检定规范制定及检定平台设施建设提供参考与借鉴。国家自然科学基金(41775027);国家自然科学基金青年科学基金(41405025

小扭口藓(Barbula indica)芽胞发育特征的实验研究

在光照培养箱中人工对照培养小扭口藓(Barbulaindica(Hook. )Spreng)的芽胞,显微镜下观察并记录其发育成配子体的全过程。结果表明:小扭口藓芽胞在3 ~4d即可萌发; 10d左右开始分化出绿丝体、轴丝体及假根; 18d,轴丝体上的侧枝顶端细胞以分生缢割的方式产生单细胞或多细胞芽胞; 40d,轴丝体上开始出现配子体原始细胞;之后,配子体原始细胞发育成桑椹状的幼小配子体。还对芽胞形态发育、生理生态及配子体发生过程的特点进行了分析和讨论。，CN 23-1480/

Calculation of passive earth pressures on retaining wall considering soil arching effects of backfill clayey soil

为解释挡土墙后填土被动土压力的非线性分布现象,在考虑土拱形状为圆弧,滑裂面采用朗肯滑裂面的基础上,给出考虑土拱效应的被动土压力系数kAWn,进而基于应力状态法及土楔形体静力平衡两种思想求解了竖向平均应力V？公式,在该基础上,给出黏性土填料下的挡土墙被动土压力分布公式、合力公式及作用点高度计算公式。通过与试验与其他方法对比,文中提出的方法得到验证。最后,研究了黏性土填料下的挡土墙被动土压力变化规律,即考虑土拱效应求得的黏性土填料的被动土压力分布呈现上小下大的指数型分布。此外,随着δ/φ(δ为墙土摩擦角,φ为内摩擦角)的增大,土拱效应逐渐增强,土压力合力点逐渐降低。The nonlinear phenomenon which the passive earth pressure behind the retaining wall of both sand and cohesive soil is nonlinear distribution could be explained by the soil arching effect theory.The formula which calculated passive earth pressure coefficient of which assumed the stress state method and the soil wedge static equilibrium method were derived considering the soil arching effect, assumed arch as circle and the angle of slip surface as Rankine's theory.Then, the distribution of lateral earth pressure considering the soil arching was derived by the above formulations; also the formulas that calculating the magnitude and the point of application of lateral passive earth pressure were derived.The accuracy of proposed method is confirmed by comparing with the experimental tests and values from existing equations.Finally, the trends of distribution and the height of total passive earth pressure of clayey soil are studied; distribution of passive earth pressures considering the soil arching is likely an exponential curve; and the height of resultant earth pressure point is gradually reducing with increasing the ratio of soil-wall friction angle to internal angle δ/φ.国家自然科学基金资助(No.51209180); 浙江省自然科学基金资助(No.Y1091175

Scanning of c-kit mutations in acute myeloid leukemia using probe melting curve method

目的用改良的杂交探针结合溶解曲线新方法检测急性髓性白血病C-kIT基因17号外显子上基因突变。方法利用PrIMEr PrEMIErV5.00、TM uTIlITyV1.3等软件设计引物探针,两个阶段探针分别针对d816、n820和n822等突变热点。取患者dnA样品5μl进行PCr扩增及熔解曲线分析,PCr扩增产物进行测序分析并与熔解曲线分析方法进行比较。结果阳性质粒灵敏度试验的结果显示,n820g的灵敏度为10%,其余突变灵敏度均能达到5%。12例Cbf-AMl标本中,5例检测为阳性,突变率为41.7%,测序结果与测序检测结果相符。结论新方法具有方便快速、灵敏度高、特异性好等特点,可用于Cbf-AMl患者的个体化诊断和治疗。Objective To detect c-kit gene mutations of acute myeloid leukemia in the hotspot exon 17 of the c-kit gene using a modified hybridization probe combining with melting curve.Methods Primer Premier v5.00 and Tm Utility v1.3 software were used to design the primers and probe,the probe contained two parts,the first segment of the probe could detect mutations around D816 and the second segment could detect mutations at N820 and N822.5 μl DNA samples from patients were used for PCR amplification and melting curve analysis.PCR products were sent to be sequenced,these results were compared with those of the mutation-positive plasmids.Results The detection sensitivity on positive control plasmids was 10% for N820G and 5% for the other mutations.c-kit mutations were identified in 5 of 12 patients with CBF-AML(41.7%),the result was confirmed by sequencing analysis.Conclusion The new method is simple,rapid,and specific with high sensitivity and could be used in individual diagnosis and treatment of CBF-AML patients

Reviews on Ecosystem Services Valuation and GIS-Based Mapping

生态系统服务价值评估是当前生态经济学领域的研究热点,为解决生态系统对人类的重要性提供了量化参考。文章论述了国内外生态系统服务价值的评估方法,分别从静态评估、动态评估和gIS技术应用三大方面进行了归纳分析,提出今后应重点关注生态系统服务价值的时空动态模型开发、评价指标体系与评价方法的标准化制订和生态系统综合管理与应用等方面的研究。Ecosystem services valuation has become one of the hotspots in the field of ecological economics,and provides quantitative evidence concerning the importance of the ecosystem for human-beings.In this paper,we first discussed ESV method in static,dynamic and GIS based respectively.We pointed out that scientific community should focus on several topics in the near future to deepen the understanding of the values of ecosystems.These topics include the development of spatially and temporarily explicit dynamic valuation models,standardization of valuation methods and payment for ES and meeting the requirements of regional ecosystem management and decision making.北京市科学技术委员会“永定河生态修复目标体系研究”(D090409004009003);北京市生态学重点学科项

急性髓系白血病NPM1基因突变检测方法的研究

本研究旨在建立检测急性髓系细胞白血病(AML)患者NPM1基因突变的方法。针对NPM1基因突变集中区域设计引物/探针,建立高分辨熔解曲线方法(HRM)和等位基因特异PCR方法(AS-PCR),通过89份AML标本进行了临床评估,并采用毛细管电泳法(CE)和测序法作为对照进行了验证。结果表明,通过上述4种方法共检出阳性标本17例(19.1%),其中A型突变15例,B型和Nm型突变各1例。上述方法中HRM法检测全面,灵敏度为5%,而AS-PCR法有一定的漏检,但灵敏度高达0.01%。结论:考虑到操作的难易程度,同时也结合临床样品的检测情况,HRM在临床上可法用于NPM1突变筛查,而AS-PCR法可用于后续的微小残留病定量检测

Electrosynthesis of Glycerol Carbonate from CO2 and Glycerol

在常温常压下，采用电化学方法，以丙三醇和CO2为原料合成了丙三醇碳酸酯，并详细考察了各种条件对该反应的影响规律. 择优条件下，目标碳酸酯的产率可达73%，远远高于普通的催化方法. 并借助循环伏安曲线研究了反应体系的电化学行为，分析了整个反应的可能历程.Glycerol carbonate was obtained from glycerol and carbon dioxide under room temperature and normal pressure by electrochemical method. The effects of synthesis conditions, such as cathode, charge, current density and temperature, on the reaction of glycerol and carbon dioxide have been investigated. The yields of 73% were produced by electrosynthesis of carbonate under the optimized condition, which is much higher than that by common catalysis method. The electrochemical behavior of the reaction system has been studied by cyclic voltammetry, through which the reaction process has been proposed.国家自然科学基金项目（No. 211730885，No. 21203066）资助作者联系地址：上海市绿色化学与化工过程绿色化重点实验室，华东师范大学化学系，上海 200062Author's Address: Shanghai Key Laboratory of Green Chemistry and Chemical Process, Department of Chemistry, East China Normal University, Shanghai 200062, China通讯作者E-mail：[email protected]