Two Epothilones from Sorangium cellulosum Strain So0157-2

目的:对粘细菌SOrAngIuM CEllulOSuM SO0157-2的化学成分进行研究。方法:通过色谱层析对提取物进行分离纯化,并通过波谱解析(一维、二维的核磁共振谱和质谱)确定了化合物的结构。结果:分别鉴定为SECO-EPOTHIlOnEA(1)和1-METHyl-SECO-EPOTHIlOnEA(2)。结论:化合物1和2都是首次从该菌株中分离得到。其中1是首次分离得到的新天然产物。AIM:To study the chemical constituents of Sorangium cellulosum So0157-2.METHODS:The chemical constituents were isolated and purified by column chromatography,and their structures were identified by spectroscopic analyses including 1D-,2D-NMR data and MS analyses.RESULTS:Two epothilones were purified and identified as seco-epothilone A(1) and 1-methyl-seco-epothilone A(2).CONCLUSION:1 and 2 were obtained from this strain for the first time,and 1 was a new natural product.supportedbyKeyProgramofInternationalCooperation;MinistryofScienceandTechnologyofChina(No.2007DFA30970);the863ProgramofChina(No.20060102Z1083)---

用细胞电泳技术检测化疗药物电性

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一种聚酰亚胺填埋双沟脊型器件沟道的制作方法，包括如下步骤：步骤1：在衬底的表面依次生长有源层和限制层，作为器件的基本结构；步骤2：在限制层的表面制作光刻图形；步骤3：光刻，在限制层的表面刻蚀出双沟脊型结构，刻蚀深度到达有源层的表面；步骤4：在双沟脊型结构内及限制层的表面涂聚酰亚胺介质层，预固化；步骤5：在聚酰亚胺介质层的表面涂光刻胶，烘干；步骤6：曝光，显影去掉双沟脊型结构上的及限制层表面的聚酰亚胺介质层，固化，完成器件的制作