Research on the Fluorescence Characteristics of Synechococcus sp.and Salt Algae

[目的]研究聚球藻和盐藻的荧光特性,确定其在特征激发、发射波长下藻浓度和荧光强度的关系,寻找一种确定藻类浓度的简便方法。[方法]以岛津rf5301型荧光分光光度计分析检测聚球藻和盐藻的激发和发射光谱,并获得它们的荧光强度与以血球计数板计数的藻浓度之间的关系。[结果]聚球藻和盐藻的激发、发射光谱有着明显的不同。在它们特征的激发、发射波长下,其浓度与荧光强度呈良好的线性关系。[结论]通过测定藻类的荧光强度就可推算出特定藻类的浓度,且该方法比血球计数板计算藻类的浓度更为便捷。[Objective] A simple determining method of the concentration of the known algae:Synechococcus sp.and salt algae,was formed through the confirmation of the relationship between its concentration and fluorescence intensity under its characteristics of excitation and emission wavelength based on research on its fluorescence characteristics.[Method] The excitation and emission spectra of Synechococcus sp.and salt algae was measured with the fluorescence spectrophotometer(RF5301) and the relationship between the fluorescence intensity and the concentration of alga based on the number at blood counting plate was gotten.[Results] There was a marked difference in the excitation and emission spectra between Synechococcus sp.and salt algae and there was good linear relationship between its concentration and the fluorescence intensity under its special excitation and emission wavelength.[Conclusion] The concentration of specific algae could be calculated through the determination of its fluorescence intensity,and the method was more convenient in the concentration-testing of algae than that of the blood cell counting sheet

Research of Oxygen Fiber-Optic Sensor with Complexes of Ru(Ⅱ) as Probe

研究了PH、温度对钌（ Ⅱ） 的配合物ru （bPy）3 Cl2 及ru （PHEn）3 Cl2（bPy ＝ 2 ，2′- 联吡啶，PHEn ＝ 1 ， 10 - 邻菲罗啉） 荧光性质的影响， 并试验了O2 对ru （PHEn）3Cl2 在水溶液、醋酸纤维素膜及多孔塑料光纤中的荧光猝灭情况.Determination of trace O 2 based on O 2 quenchingfluorescence of complexes of Ru(Ⅱ) has been an advanced research region in fiber-optic chemical sensor for recent years.Effects of pH,temperature on fluorescence of Ru(bpy) 3 Cl 2 and Ru(phen) 3 Cl 2 are described in this article.And O 2 quenching fluorescence of Ru(phen) 3 Cl 2 in water,celluloseacetate film,plastic porous fiber-optic is investigated too.国家863计划资助!（818－Q－09

Ammonia-Sensitive Porous Plastic Fiber Optical Probe Utilising Tri-iodo-fluorescein as Fluorescence Indicator

研究了以甲基丙烯酸甲酯、双甲基丙烯酸四乙二醇酯和庚烷为聚合原料， 藻红为荧光指示剂对氨敏感的多孔塑料光纤探头， 同时探讨PH 及氨对其荧光强度的影响.Ammonia-sensitive porous plastic fiber optical probe, which is polymerized by methyl methylacrylate,tetraethylene-glycol bis(methylacrylate) and heptane,utilising tri-iodo-fluorescein as fluorescent indicator is invistigated;effect of pH and ammonia is also discussed.国家863计划资助!（818－Q－09

Determination of Trace Ammonia in Water by Fluorescence Spectrophotometry Combining with Flow-Injection Analysis

研究了在碱性介质中， 基于氨（ 铵离子） 与邻苯二甲醛及2 - 巯基乙醇反应生成强荧光性吲哚取代衍生物的体系测定微量氨（ 铵离子） 的流动注射荧光分析法. 非离子表面活性剂曲拉通X-100 （TrITOn X- 100） 对该体系具有良好的增稳作用. 研究了该体系测定氨（ 铵离子） 的最佳实验条件. 测定氨（ 铵离子） 的线性范围0 ~0-7Mg/l， 相对标准偏差为1-3 % （ CnH3 ＝ 0-2Mg/l，n ＝ 11） ，检测限为0-0012Mg/l.A method is described for determination of trace ammonia by fluorescence spectrophotometer combining with flow-injection Analysis.The method is based on the system:in base media,ammonia reacts with o-phthaladehyde(OPA) and 2-mercaptoethanol(ME) producing derivative of indol which has intensive fluorescence.Nonionic surfactant Triton X-100 enhances stability in the system.The optimum reaction condition is investigated.A linear relationship between ammonia concentration and fluorescent intensity is observed over the range 0 1～0 7mg/L with a relative standard deviation(RSD) of 1 3% at ammonia concentration of 0 2 mg/L(n=11).The limit of determination is 0 001 2mg/L.国家863计划资助!（818－Q－09

Determination of Trace PO_4--(-3) by Quenching Fluorescence with Flow Injection Analysis

研究了以铝- 桑色素为荧光试剂， 在酸性条件下利用荧光熄灭法， 并结合了流动注射分析测定了水中微量磷酸根离子的方法. 测定PO3 -4 的线性范围1 ~9 Mg/l， 检测限为0-002 Mg/l， 1H可以测定60 份样品， 相对标准偏差为1-09 % .A method is investigated to determinate trace PO 4 3- in acid solution based on oxygen quenching aluminium-morin fluorescence intensity combining with flow-injection analysis.The linearship is between 1～9mg/L with the limit of detection 0 002mg/L.60 samples per hour can determinated by this method with RDS 1 09%.国家863计划资助!（818－Q－09

马铃薯重离子辐射育种研究

在2000—2005年将杂交技术和重离子辐射相结合,采用55MeV/u的40Ar17+离子对马铃薯杂交种子和微型薯进行了不同剂量的贯穿辐射或离子注入处理。结果表明,对马铃薯微型薯进行重离子辐射能显著提高块茎的产量。而杂交技术和重离子辐射相结合是一种更高效的育种方式,不仅能提高块茎的增产幅度,还能改善其加工品质。微型薯重离子辐射的最佳剂量为60Gy,杂交种子辐射的适宜剂量范围在60—120Gy之间。通过对辐射后代的多年选育,获得了几个产量显著高于对照、品质优良的马铃薯新品系

Study of pH Sensitive Membrane Based on Ion Pairs Technique

提出一种新型pH吸收传感膜。指示剂溴酚蓝与季铵盐阳离子以离子对的形式结合,均匀分布于增塑的PVC膜中。该传感膜测定pH的范围为4.0～6.0。对传感膜的制备方法及响应时间、可逆性、重现性等进行了研究。并应用于实际样品的测定,获得了比较好的结果。A new pH sensitive membrane based on ion pairs technique was developed. Ion pairs indicator, which was formed by bromo-phenol blue reacting with quaternary ammonium cetyl trimethyl ammonium (CTA) bromide, was homogeneously immobilized on plas-ticized PVC membrane. The sensitive membrane covers the pH range between 4.0 and 6.0. Preparation, response time, reversibility and reproducibility of the sensitive membrane were investigated. The sensitive membrane was used to determine the effluents in the mixture pool of antibiotic factory.教育部《高等学校骨干教师资助计划》项目;; 福建省自然科学基金(D9910009);; 福建省科技三项资助项目(K99037);; 福州大学科技发展基

Study of pH sensitive membrane based on ion pairs technique

A new pH sensitive membrane based on ion pairs technique was developed. Ion pairs indicator, which was formed by bromophenol blue reacting with quaternary ammonium cetyl trimethyl ammonium (CTA) bromide, was homogeneously immobilized on plasticized PVC membrane. The sensitive membrane covers the pH range between 4.0 and 6.0. Preparation, response time, reversibility and reproducibility of the sensitive membrane were investigated. The sensitive membrane was used to determine the effluents in the mixture pool of antibiotic factory