[目的]研究聚球藻和盐藻的荧光特性,确定其在特征激发、发射波长下藻浓度和荧光强度的关系,寻找一种确定藻类浓度的简便方法。[方法]以岛津rf5301型荧光分光光度计分析检测聚球藻和盐藻的激发和发射光谱,并获得它们的荧光强度与以血球计数板计数的藻浓度之间的关系。[结果]聚球藻和盐藻的激发、发射光谱有着明显的不同。在它们特征的激发、发射波长下,其浓度与荧光强度呈良好的线性关系。[结论]通过测定藻类的荧光强度就可推算出特定藻类的浓度,且该方法比血球计数板计算藻类的浓度更为便捷。[Objective] A simple determining method of the concentration of the known algae:Synechococcus sp.and salt algae,was formed through the confirmation of the relationship between its concentration and fluorescence intensity under its characteristics of excitation and emission wavelength based on research on its fluorescence characteristics.[Method] The excitation and emission spectra of Synechococcus sp.and salt algae was measured with the fluorescence spectrophotometer(RF5301) and the relationship between the fluorescence intensity and the concentration of alga based on the number at blood counting plate was gotten.[Results] There was a marked difference in the excitation and emission spectra between Synechococcus sp.and salt algae and there was good linear relationship between its concentration and the fluorescence intensity under its special excitation and emission wavelength.[Conclusion] The concentration of specific algae could be calculated through the determination of its fluorescence intensity,and the method was more convenient in the concentration-testing of algae than that of the blood cell counting sheet
