Comparative study of large yellow croaker (Pseudosciaena crocea) and marine medaka (Oryzias melastigma) Hepcidin gene expression and peptide functions

抗菌肽是一类从生物体中分离的小分子多肽，具有抗细菌，真菌，病毒，肿瘤和寄生虫等多种作用。由于抗菌肽来源于生物体本身，同时病原菌不易对其产生耐药性，所以被视为一种极有潜力的抗生素的替代品。Hepcidin抗菌肽是鱼类抗菌肽家族中的重要一种，鱼体内的Hepcidin更是鱼类先天免疫因子的重要组成部分。本研究运用分子生物学技术对养殖鱼类大黄鱼（Pseudosciaenacrocea）Hepcidin和模式动物海水青鳉（Oryziasmelastigma）Hepcidin的基因表达特性与功能进行了比较研究和探讨，主要研究成果如下： 1. 克隆获得了源于海水养殖大黄鱼Hepcidin（Pc-hepc）...Antimicrobial peptides (AMPs) are a group of small peptides amplified from organisms which have anti-bacterial activity, anti-fungi activity, anti-virus activity, anti-tumors activity, etc. AMPs are thought to be very good substitutes for antibiotics that they can defense pathogens invading with less drug tolerance for they are derived from organism themselves. Hepcidin is an important AMP family,...学位：理学博士院系专业：海洋与环境学院环境科学与工程系_环境科学学号：2262007015392

Prevention for Seismic Secondary Geological Disasters and Aseismic nalysis for Rural and School Buildings in Xiamen City

2008年5月12日，我国四川省汶川县发生里氏8.0级特大地震。此次大地震造成了大量的房屋破坏，农村房屋的破坏最为严重。农村房屋抗震能力低下、抗震措施不足是最主要的原因。学校建筑在这次地震中也受到不同程度的破坏，作为公共性建筑和避难场所，这样的破坏会造成很大的损失，其根本的原因是学校建筑结构体系的缺陷。除了以上两种原生灾害外，地震次生地质灾害所带来的破坏也极为严重。 地质情况不佳、农村房屋抗震性能差、学校在役建筑抗震能力低恰恰是厦门地区存在的抗震薄弱环节，如果地震来临，也将遭受严重的破坏，厦门市这几个方面的系统研究还比较少，为此，本文针对这三个方面做了深入的研究和探讨。 本文首先分析了地震...A destructive earthquake with the seismic magnitude of 8.0 degree occrued in May 12 2008, known as Wenchuan earthquake in Sichuan Province. This severe earthquake led to the collapse and severe damage to huge numbers of buildings, especially the rural houses. The main reason is lower seismic defence capacity and deficient seismic measures. As a public building and evacuee shelter, school buildings...学位：工学硕士院系专业：建筑与土木工程学院土木工程系_结构工程学号：2532007115219

论我国对外反倾销实践的特点及存在的问题

反倾销是保护国内产业健康发展,维护经济安全的一个重要手段。本文分析了入世后中国对外反倾销的特点,并分析了现存反倾销实践中所存在的问题。最后,笔者对如何完善反倾销实践及应注意的问题,提出了自己的看法

提高闽南地区农村房屋抗震能力的探讨

本文从农村房屋震害分析入手,针对闽南地区农村房屋结构特点及在抗震设防中存在的问题,提出了改善农村房屋抗震性能的具体措施。本文还就新建农村房屋的抗震设防及既有农村房屋的加固改造提出相关建议

典型框架式学校建筑的抗震性能分析

本文在研究汶川地震灾区学校建筑震害的基础上,探讨了中小学校典型框架结构即外廊悬挑式单跨框架结构的抗震性能。基于静力非线性方法,用ETABS对典型框架结构进行弹塑性分析,对不同水准下结构的出铰顺序,出铰数量,侧向位移等进行对比,得出相应的抗震性能评价,本文还对该框架结构的薄弱部位进行分析,提出加固改造的相关建议。本文的研究工作对提高学校建筑的抗震能力具有实用价值

地震次生地质灾害的分析及防治对策

本文基于对5.12汶川大地震灾后现场灾情以及灾后抢险、恢复重建工作等方面的调查,分析了地质灾害及造成这些地质灾害的原因,并通过分析,提出防治地质灾害的措施。本文还就减轻地质灾害、提高规划选址水平、增强防灾意识等提出建议,供今后在工程建设时参考

Relative Quantification of Hepcidin Gene Transcripts in Pseudosciaena crocea

以大黄鱼(PSEudOSCIAEnA CrOCEA)管家基因18S rrnA和β-ACTIn作为内参基因,分别比较2个内参基因建立的相对定量曲线,最终确立以18S rrnA为参比基因,定量分析大黄鱼的HEPCIdIn抗菌肽基因.该相对定量分析方法所得结果与nOrTHErn-blOT方法一致.应用建立的HEPCIdIn基因的实时荧光相对定量研究方法,对大黄鱼头肾中的HEPCIdIn基因转录物进行相对定量,为今后开展鱼体内免疫相关基因的表达特性、诱导机制等工作奠定研究基础.同时,克隆得到的大黄鱼β-ACTIn基因和18S rrnA基因片段已提交基因库,并获得登录号.Two house-keeping genes,18S rRNA and β-actin,were cloned as endogenous genes for relative quantification of an antimicrobial peptide hepcidin in large yellow croakers(Pseudosciaena crocea) using the real-time RT-PCR.In comparision of the slopes of the relative standard curves between the two endogenous genes and the target gene,a relative quantification method for hepcidin gene using 18S rRNA as the calibrator gene was established.Results obtained with this method were consistent with the rusults from Northern-blot,suggesting feasibility and effectiveness of the method.This paper provides an effective method to investigate expression patterns and induction profiles of the hepcidin gene in large yellow croakers,and other immunity-related genes in fish bodies.The cloned fragments of 18S rRNA and β-actin genes from the large yellow croaker have been submitted to the GenBank,which have provided accession numbers.福建省科技计划重点资助项目(2007I0022);上海市高校选拔优秀青年教师科研专项基金资助项目(SHU08021);上海大学创新基金资助项目(A10011109006

Construction of SSH library from haemocyte of variously colored abalone challenged with bacteria and differential expression analysis of macrophage expressed protein

以雌性杂色鲍为对象,以大肠杆菌、副溶血弧菌、溶壁微球菌、表皮葡萄球菌、金黄色葡萄球菌的混悬液做为攻毒菌,利用抑制性差减杂交(SSH)技术构建细菌攻毒的杂色鲍血淋巴细胞SSHcDNA文库。随机挑取生长菌落110个克隆子,进行菌液PCR鉴定,计算文库重组率为98.18%,文库容量为1.37×106pfu。将重组子测序,经BLAST一致性搜索比对分析,有一重组片段含有穿孔素(Perforin)保守结构域,为巨噬细胞表达蛋白(MEP)类穿孔素部分cDNA序列,片段大小为1551bp,连续编码517个氨基酸残基,申请GenBank登录号为EU272049。经半定量PCR和荧光定量PCR差异显示分析,发现在细菌感染状态下MEP基因在血淋巴细胞中存在明显的上调表达现象。Abalones are considered to be the most precious delicacy from the sea, and become very important commercial seafood in aquaculture worldwide. Variously colored abalone (Haliotis diversicolor Reeve, 1846) has been widely cultured on the southeast coast for more than twenty years. However, abalone culture frequently suffers from bacterial infection and mass mortality of reared abalones causes serious economic losses. Unfortunately, knowledge of the defense mechanism in this animal is still lacking. In this study, using suppression subtractive hybridization (SSH) technology, a forward SSH li-brary was constructed from haemocytes of H. diversicolor, with the content of 1.37×106 pfu and the recombinant rate of98.18%. After the recombinant plasmids were sequenced, partial cDNA of macrophage expressed protein (MEP) was recognized based on BLAST searches in NCBI, with the size of 1 551 bp, and continuously encoding 517 amino acids. Semi-quantitative PCR and quantitative real-time PCR results showed that MEP cDNA was distinctly up-regulated in haemocytes of the bacterial-challenged group compared to the unchallenged group. The gene information obtained from this library will provide new insights into the immune mechanism of H. diversicolor and facilitate future study of target genes involved in the response to invading microorganisms.国家高技术研究发展计划项目(863计划)(编号:2007AA091406)资助~

The Research on Removal of Agricultural Export Subsidy by Political Economics

农产品贸易是当今世界自由贸易的重灾区，是贸易保护政策最明显的领域。它一直游离于以GATT为核心的多边贸易体制之外。直到乌拉圭回合，《农业协定》才确立了谈判的三大核心内容。作为核心之一的农业出口补贴是改变贸易流向的一个有效手段，是造成贸易扭曲的原因之一。但是农业出口补贴涉及发达国家之间、发达国家与发展中国家之间错综复杂的矛盾，是多哈回合谈判的重要内容，因而谈判过程甚为曲折，成为了各国争论的焦点。但是传统的经济学理论无法做出很好地解释。 传统的经济学理论从福利经济学角度出发，缺乏一种独立的政治决策理论，认为政府在制定和执行公共政策时表现为外生的行动者，认为政治决策是经济决策的外生变量，从而将政治...There are still many trade barriers in agricultural trade which is out of the domination of multinational trade system based on GATT. On the Uruguay Rounds, the members of GATT came to Agreement of Agriculture of which export subsidy became an important part. As an effective way to change the trade flow, export subsidy not only reflects complicated conflicts, but also is an important part of Doha ...学位：经济学硕士院系专业：经济学院国际贸易系_国际贸易学学号：20041301