靶向HIV-1 vif的高效siRNA的筛选及慢病毒介导的体外抗病毒研究

RNAi技术在艾滋病治疗研究中已展现出巨大的潜力,兼具高效抑制特性和保守性的siRNA靶位是其获得成功应用的重要基础。本研究选择以HIV-1 vif基因为靶区筛选高效保守的RNAi序列,共选择设计了30个识别不同位点的siRNA序列,以pSUPER为载体构建了相应的shRNA表达质粒。通过与pNL4-3质粒在293FT细胞中进行共转染抑制实验,以及对初筛获得的高效序列进行保守性分析显示siRNA-vif37序列具有高效抑制效率和较好的保守性特征。通过与pGL3-vif报告质粒的共转染实验证明siRNA-vif37具有vif基因抑制特异性。带有shRNA-vif37表达元件的重组慢病毒转导后的MT-4细胞在HIV-1NL4-3体外攻毒实验中可显示出较有效的抑制病毒复制的能力,本研究进一步对转导后细胞进行克隆化筛选,获得稳定整合shRNA-vif37表达元件的MT-4-vif37细胞克隆,该细胞具有显著的抑制病毒复制的能力,在高攻毒剂量下仍可获得良好的抑制效果。本研究为进一步应用RNAi技术进行新型艾滋病治疗方法研究提供了重要基础

Legislative Defects and Improvement of food safety offenses under the Risk Society

风险社会下,科技与食品的结合、利益追求的激烈化,我国食品安全犯罪在犯罪构成的主观、主体要件上出现了诸多与传统犯罪不一样的特点,食品安全犯罪的归责陷入困境。增设严格责任主义下的"风险犯"和提倡食品安全犯罪团体责任显得十分必要。食品安全犯罪立法体系上犯罪客体划分混乱、法规衔接不协调造成的司法运作困境又要求我国必须明确食品安全犯罪客体标准,整合食品安全犯罪于同一章节,设立危害食品安全罪。合理使刑罚提前介入,适当扩大食品安全犯罪圈,才能真正有效地实现食品安全的保护。Under the risk society, technology and food combination, the fierce pursuit of interests, China＆#39;s food safety crimes and tra-ditional crimes there have been many different features on the subjective, the main elements of the crime constituted a crime imputation troubled food safety .＆quot;Risk committed＆quot;criminal groups and advocates for food safety responsibility for the creation of strict liability under the doctrine is very necessary. Crime legislation on food safety systems division of criminal object confusion, uncoordinated regulatory con-vergence difficulties caused by the functioning of the judiciary and the requirements of the object of the crime must be clear standards for food safety, the integration of food safety crimes in the same chapter, the establishment of food safety hazards sin. Make a reasonable penalty early intervention, appropriate expansion of food safety crime ring in order to achieve a truly effective food safety protection

On the Ambiguity of Victims Self-Responding to Prosecutien Theory in the Criminal Law

被害人自我答责理论模糊性的主要表现:一是被害人自我答责理论的基本内涵不清晰;二是被害人自我答责判断标准不统一;三是被害人自我答责的判断条件缺乏可操作性。发现和正视该理论存在的模糊性是继续完善该理论的首要任务,如果说要继续在中国提倡被害人自我答责理论,就必须继续研究和解决该理论中的各种混乱、不协调。The ambiguity of Self-Responding to Prosecutien Theory of victims exists in the following three aspects： 1. the fundamental connotation of victims Self-Responding to Prosecutien theory is fuzzy; 2. there is no unified criteria in the theory; 3. the condition for judgment lacks maneuverability. It is believed that the top priority to perfect the theory is to find out its ambiguity.Only after the chaos and inconsistence in this theory are solved can it be practiced in China

风险刑法正当性探析

风险社会是当代社会现象的客观现实描述,风险刑法成为应对风险管理和控制的重要工具。作为刑法核心内容的"罪责原则"和"法益保护原则"也因此成为风险刑法正当性的两大重要依据

碱式碳酸镍浆化氢还原制取镍粉的研究

针对硫酸铵溶液体系氢还原处理红土矿存在的问题,提出了从碱式碳酸镍直接浆化氢还原制取镍粉的新工艺。对流程中的主要工序进行了小型试验,对浆化氢还原还进行了50立升高压釜的扩大试验。试验结果表明:文中提出的工艺流程比传统的煅烧法,电解法以及硫酸铵溶液体系氢还原优越。它的主要特点是:流程简单,镍钴回收率高,能耗低,产品质量合格。可以认为采用碱式碳酸镍直接浆化氢还原制镍粉将是红士矿、硅酸镍矿湿法冶金的一个新方向

石墨烯改性重防腐涂料在世界最高输电铁塔的防腐应用

针对世界第一380 m输电铁塔在临海海洋大气腐蚀环境的防腐耐候需求,设计石墨烯改性重防腐涂料体系(包括环氧石墨烯防腐底漆、环氧石墨烯阻隔中间漆和氟碳耐候面漆,其中氟碳面漆采用红白相间颜色,具有防腐和航空标识功能)对输电铁塔进行防护涂装。通过实验室配方优化、石墨烯改性重防腐涂料性能检测和示范工程涂装,最终定型涂料配方体系。同时针对临海环境室外涂装工况,介绍石墨烯改性重防腐涂料在铁塔镀锌管件的施工工艺和施工经验

JUNO Sensitivity on Proton Decay p→νˉK+p\to \bar\nu K^+ Searches

The Jiangmen Underground Neutrino Observatory (JUNO) is a large liquid scintillator detector designed to explore many topics in fundamental physics. In this paper, the potential on searching for proton decay in $p\to \bar\nu K^+$ mode with JUNO is investigated.The kaon and its decay particles feature a clear three-fold coincidence signature that results in a high efficiency for identification. Moreover, the excellent energy resolution of JUNO permits to suppress the sizable background caused by other delayed signals. Based on these advantages, the detection efficiency for the proton decay via $p\to \bar\nu K^+$ is 36.9% with a background level of 0.2 events after 10 years of data taking. The estimated sensitivity based on 200 kton-years exposure is $9.6 \times 10^{33}$ years, competitive with the current best limits on the proton lifetime in this channel