DIAGNOSIS OF TRICHINOSIS BY ELISA WITH P49/GST ANTIGEN

目的以纯化的融合蛋白 p49/GST为抗原建立ELISA检测方法。方法对一批试验血清进行间接ELISA检测。结果 19份人工感染鼠血清、5份人工感染猪血清、4份旋毛虫病猪血清、4份病人血清呈IgG抗体阳性 ,2 1份人工感染鼠血清呈IgM抗体阳性 ,而正常对照血清及 30 0份屠宰场待检猪血清均呈阴性反应 ,其结果与常规压片法结果相符。结论融合蛋白p49/GST对于研制旋毛虫病的诊断抗原具有的潜在应用价值Aim To establish ELISA detecting method for the specific antibodies of Trichinella spiralis.Method A series of confirmed trichinosis sera were detected with ELISA with fusion protein p49/GST.Results Positive results of IgG antibody were found in 19sera of experimental infected mouse and 3sera of infected swine and 4 sera of patients;positive results of IgM antibody were found in 21 sera of experimental infected mouse .All normal sera were negative. Conclusion The ELISA with p49/GST can be regarded as a sensitive, specific immunological method for the diagnosis of trichinosis.福建省重点(农医 )项目资助!(项目号 :95 -Z -15 0

A TWO-PARTICLE TURBIDIMETRIC LATEX IMMUNOASSAY FOR THE DETECTION OF SPECIFIC ANTIBODIES OF TRICHINELLA SPIRALIS

目的　建立旋毛虫病的快速检测技术。方法　基因工程抗原包被有色乳胶颗粒，抗抗体包被磁性颗粒，在抗体存在下形成抗原－ 乳胶－ 抗体－ 抗抗体－ 磁性颗粒的复合场，在磁场作用下沉淀下来，从而达到快速检测旋毛虫相关抗体的目的。结果　１９ 份人工感染鼠血清、５ 份人工感染猪血清、３ 份旋毛虫病猪血清、４ 份病人血清均呈阳性反应，而对照血清均为阴性反应；该方法在鼠感染旋毛虫后第五天可测出ＩｇＭ 抗体，第九天可测出ＩｇＧ抗体。结论　本检测技术简便易行，不需专用设备，可望成为一种快速诊断旋毛虫病的有效方法。Aim To establish a rapidly detecting method for the specific antibodies of Trichinella spiralis Method Latex was coated with p49/GST and polystyrene beads(Dynabeads)were coated with anti-antibodies SAM-IgG、SAH-IgG、RAP-IgG or SAM-IgM Then the test serum was incubated with two particles for 30 min at room temperature with slowly shaking After sedimentation of the polystyrene beads with a magnet,the turbidity of the resultant latex suspension was estimated with eyes or measured spectrophotometrically at a wavelength of 400nm Results Positive result of IgG antibody were found in 19 sera of experimental infected mice,3 sera of experimental infected swine and 4 sera of patients The IgM and IgG can be check up in mice after 5 and 9 days after experimental infection The result agreed with those obtained by ELISA Conclusion A two-particle turbidimetric latex immunoassy established by this study is a rapid,easy and precise method for the diagnosis of the trichinosis福建省科委(农医)项目!(95-Z150

威布尔分布随机载荷下风电机组行星轮系疲劳强度分析

为了快速准确地评估风电机组行星轮系疲劳寿命，根据行星轮系输入转矩与风速的分布关系，编制了不同风速下对应的二参数威布尔随机载荷谱，并用雨流计数法对载荷谱进行统计处理；在ANSYS中对行星轮轮系进行有限元分析，得到齿轮结构应力响应；依据Minner线性累积损伤理论，结合nCodeDesignLife对行星轮系进行疲劳寿命估算。着重分析了风电机组在启动风速、额定风速及年平均风速工况下的行星轮系疲劳寿命，获得各齿轮疲劳损伤状况，为风电机组行星轮系疲劳强度设计提供理论依据

Electrorheological Characters of Polyaniline Suspensions

将聚苯胺（ＰＡｎ）作为分散粒子，电绝缘油作为分散介质组成电流变流体（ＥＲＦ），研究了电场强度（Ｅ），温度（Ｔ）等因素与ＥＲＦ的静态屈服应力（τＳ）和电流密度（ｉ）的关系．结果表明：τＳ和ｉ随电场强度的变化皆成指数关系（τＳ∝Ｅ０．７，ｉ∝Ｅ２．１８）．而当施加一个恒电压于ＥＲＦ时，由于极化作用ＥＲＦ将发生介质吸收，电流的衰减速度方程可表示为：ｉ＝（Ｅ／Ｒ）ｅｘｐ（－ｔ／ＲＣｄ）Polyaniline (PAn) as the dispersive particles and insulating oil as the dispersive medium, the electrorheological fluid (ERF) is constituted. Relations between the factors of electric field strength ( E ), temperature ( T ) and the static yield stress ( τ S ), current density ( i ) of ERF are investigated. The results show that the changes of τ S and i with electric field intensity have emerged in the exponential relation, i.e, τ S∝E 0.7 , i∝E 2.18 . Otherwise, when exert a constant voltage on ERF, owing to the polarization, the absorption of medium of ERF have taken place. Therefore, the attenuation rate equation of current can be show as i=(E/R) exp (-t/RC d)作者联系地址：湘潭大学化学系Author's Address: Dept. of Chem., Xiangtan Univ., Hunan 41110

The Cloning of complementary DNA encoding p49 ES antigen of Trichinella spiralis

应用dnA重组技术将编码旋毛虫肌幼虫49kdA抗原的基因克隆于大肠杆菌中.设计特异的PCr引物，用PT-PCr技术直接从旋毛虫肌幼虫总rnA中反转录并扩增出约1.1kb的靶dnA.用bAMHI和ECOrI酶解后将其克隆到质粒载体PuC19中，用X-gAl培养基筛选重组子.该重组子经相同的核酸内切酶水解获得约2.7kb和1.1kb两个片段，分别与PuC19和目的基因的大小相同，目的基因经序列分析并与文献报道的序列比较发现具有97.8%的同源性.Complementary DNA(cDNA) encoding 49KD ES antigen of Trichinella spiralis muscle larvae was cloned in E.coli by recombinant DNA technology.Aabout1.1kb DNA fragment was obtained by RT PCR from total RNA of Trichinella spiralis using a specific 5′ end 3′ end primer.PCR product was cleaved with restriction enzymes EcoRI and BamHI and ligated into the pUC19 vector.The cloning was screened by X gal medium.When the recombinant DNA was cleaved by the same enzynes, 1.1kb and 2.7kb fragments were obtained, which were similar to the langth of PCR product and of pUC19 vcvtor.There is 97.8 percent homologous between the sequence of PCR product and the sequence which reported in literature by sequencing analysis.福建省重点（农医）资

基于魔芋葡甘聚糖微球的胶原覆层型微载体的制备研究

以魔芋葡甘聚糖(KGM)微球为基质,用1,4-丁二醇二缩水甘油醚将KGM微球进行活化,将胶原覆层到微球上,对胶原覆层进行再次交联,得到覆层均匀、稳定的微载体。通过四因素三水平的正交回归组合试验设计,考察了活化时间、蛋白质用量、偶联时间、交联剂用量对微载体细胞培养效果的影响。以Vero细胞培养效果为指标,制备胶原包被微载体的最佳工艺为活化时间5 h、蛋白用量1∶0.1(球∶蛋白)、偶联时间5 h、交联剂用量每1gKGM加入0.5ml交联剂。在最优制备条件下,培养Vero细胞最大细胞密度可达到1.7×106cells/ml,证明了胶原覆层的KGM微球作为动物细胞培养的微载体具有可行性

基于魔芋葡甘聚糖微球的胶原覆层型微载体的制备研究

以魔芋葡甘聚糖(KGM)微球为基质,用1,4-丁二醇二缩水甘油醚将KGM微球进行活化,将胶原覆层到微球上,对胶原覆层进行再次交联,得到覆层均匀、稳定的微载体。通过四因素三水平的正交回归组合试验设计,考察了活化时间、蛋白质用量、偶联时间、交联剂用量对微载体细胞培养效果的影响。以Vero细胞培养效果为指标,制备胶原包被微载体的最佳工艺为活化时间5 h、蛋白用量1∶0.1(球∶蛋白)、偶联时间5 h、交联剂用量每1gKGM加入0.5ml交联剂。在最优制备条件下,培养Vero细胞最大细胞密度可达到1.7×106cells/ml,证明了胶原覆层的KGM微球作为动物细胞培养的微载体具有可行性

基于魔芋葡甘聚糖微球的胶原覆层型微载体的制备研究

以魔芋葡甘聚糖(KGM)微球为基质,用1,4-丁二醇二缩水甘油醚将KGM微球进行活化,将胶原覆层到微球上,对胶原覆层进行再次交联,得到覆层均匀、稳定的微载体。通过四因素三水平的正交回归组合试验设计,考察了活化时间、蛋白质用量、偶联时间、交联剂用量对微载体细胞培养效果的影响。以Vero细胞培养效果为指标,制备胶原包被微载体的最佳工艺为活化时间5 h、蛋白用量1∶0.1(球∶蛋白)、偶联时间5 h、交联剂用量每1gKGM加入0.5ml交联剂。在最优制备条件下,培养Vero细胞最大细胞密度可达到1.7×106cells/ml,证明了胶原覆层的KGM微球作为动物细胞培养的微载体具有可行性