Functional analysis of Agaricus bisporus para-aminobenzoic acid synthase in Arabidopsis thaliana

孢蘑菇（Agaricusbisporus）是一种产量高、消费量大、栽培广泛的食用菌。不耐高温是限制其人工栽培的主要因素。因此，深入了解双孢蘑菇的耐温机制和培养新型种质优良的耐高温菌株，具有十分重要的生产价值和理论意义。本实验室通过比较耐温型双孢蘑菇02菌株和常规双孢蘑菇8213菌株之间的转录组表达差异，筛选出一个与耐温相关的基因，对氨基苯甲酸（PABA）合酶基因（Pabs）。该基因编码的PABA合酶（PABS）参与双孢蘑菇中PABA的生物合成。PABA是合成叶酸的一个重要前体，而叶酸是生物体内一碳单位的重要受体和供体，在一碳单位（C1代谢）转运过程中起辅酶作用；PABA还可以显著提高双孢蘑菇的...Agaricus bisporus is a kind of edible mushroom. It is widely cultivated as it has high yield property and is consumed largely. Temperature is the main restricted factor for its growth. It thus has great economic and ecological values to study its thermotolerance mechanism and to breed a novel thermotolerant strain. Comparing the differentially expressed genes between thermal-insensitive A.bisporus...学位：理学博士院系专业：生命科学学院_微生物学学号：2162010015391

Study on the Photocatalytic Degradation of Oxytetracycline by Graphene/TiO2/Zeolite compounds

环氧四环素(Oxytetracycline，OTC)又称土霉素，属于四环素类广谱抗生素，作为抗菌剂和饲料添加剂被广泛地应用于人类和动物，具有潜在的生态毒性，目前尚未找到一种理想而有效的处理方法。本研究采用光催化氧化法对其进行光催化降解实验。TiO2以其化学稳定性、无毒性、价格低廉、光催化活性高等优异性能被广泛研究。但因其颗粒过小，易团聚，不易与水分离，吸附能力差，需紫外光激发而受到限制。 本研究以具有大表面、高吸附性能的沸石为载体，以具有优异的导电性能、较高的比表面积、可最大限度地拓展光催化剂对可见光的响应范围的石墨烯掺杂，采用固态分散法制备石墨烯/二氧化钛/沸石(Graphene/TiO2...Oxytetracycline(OTC), an important member of tetracycline and is popularly used in humans and animals, which is reported to be a toxic threat to the ecosystem. It is important to find the treatment method which has high removal efficiency for actual use since OTC are not biodegradable. In this study, the photocatalysis was used to degrade the OTC. TiO2 is a promising photocatalyst which is stable...学位：理学硕士院系专业：环境与生态学院_生态学学号：3332013115170

对氨基苯甲酸在拟南芥根生长发育中对生长素运输的调控

为探究对氨基苯甲酸(PABA)影响拟南芥(Arabidopsis thaliana)根部生长发育的作用机制,以野生型和DR5∷GUS转基因拟南芥为实验材料,研究了PABA外源处理对根系形态结构和生长素运输的影响.结果表明:200μmol/L PABA可以明显抑制拟南芥幼苗主根生长并促进侧根和根毛的发育.PABA可增强生长素类似物2,4-D对幼苗主根生长的抑制作用和对根毛伸长的促进作用;相反,PABA可在一定程度上减弱生长素运输抑制剂NPA对幼苗主根和根毛生长发育的抑制作用.此外,PABA可诱导拟南芥根尖生长素的积累以及生长素极性运输相关基因PIN1、PIN3和AUX1的表达.综上所述,PABA可调节根部生长素运输和分布,在拟南芥根生长发育过程中起重要作用.国家自然科学基金(30571259

对氨基苯甲酸在拟南芥根生长发育中对生长素运输的调控

为探究对氨基苯甲酸（PABA）影响拟南芥根部生长发育的作用机制，通过以野生型拟南芥和DR5::GUS转基因拟南芥为实验材料,研究了PABA外源处理对根系形态结构和生长素的运输和分布的影响。结果表明：200 μmol/L PABA可以明显抑制拟南芥幼苗主根生长，并促进侧根和根毛的发育。PABA可增强生长素类似物2,4-D对幼苗主根生长抑制的影响，促进根毛伸长；相反，PABA可在一定程度上恢复生长素运输抑制剂NPA对幼苗主根和根毛生长发育的抑制作用。此外，PABA可以诱导拟南芥根尖生长素的积累，并影响生长素极性运输相关基因PIN1、PIN3和AUX1的表达。综上所述，PABA可调节根部生长素运输和分布，在拟南芥根生长发育过程中起重要作用。国家自然科学基金(30571259

Relative stability of the key enamine, oxazolidinone, and imine intermediates in some proline-catalyzed asymmetric reactions

Many proline-catalyzed asymmetric addition reactions with ketones as substrates were assumed to involve a key intermediate, an enamine, produced by the condensation of proline and ketone. In this paper, the key intermediate enamines derived from L-proline and cyclohexanone (or acetone) as well as the corresponding oxazolidinone and imine tautomers have been investigated by means of density functional calculations at the B3LYP/6-311+G** level. The predicted order of stability for these tautomers is oxazolidinones > enamines > imines in gas phase and oxazolidinones > imines > enamines in aprotic THF solvent. This prediction explains why enamine intermediate can not be observed experimentally. The predicted energy/enthalpy difference between the formal oxazolidinone structure and the zwitterionic imine, structures is very small in THF solvent, suggesting the oxazolidinone-to-imine tautomerization can be readily induced in solvent. C-13 NMR chemical shifts of the oxazolidinone and imine structures have been computed and used to explain the experimental NMR spectra observed in oxazolidinone-to-imine tautomerization induced by protic solvent

Intelligent design of hammer mill based on SolidWorks

为适应现代技术发展及产品快速更新的要求,针对机械产品中装配体结构的快速造型设计问题,以SOlIdWOrkS2011为开发平台,运用VC++编程语言,建立了锤片式粉碎机的装配体实体模型,研究了以dll开发方式进行锤片式粉碎机产品智能化与自动化设计的方法,完成了用户界面的开发。以尺寸驱动为特征,参数化驱动实现锤片式粉碎机的重新建模,自动完成了整体模型的变动,并结合实例具体说明程序代码的设计,生成了自主设计的一套锤片式粉碎机参数化建模插件。研究结果表明:该智能化设计系统可以快速、自动修改锤片式粉碎机装配下零件的参数,并且输出形象直观的装配体三维实体模型,从而实现锤片式粉碎机设计的智能化及自动化,加快了产品设计过程,提高了效率。In order to adapt the development of the modern technology and the fast renewal of the product request,aiming at rapid modeling design of the assembly in mechanical product,based on SolidWorks2011 development platform and made use of VC + + programming language embedded in SolidWorks,the solid model of the hammer mill was established,and the intelligent and automatic design method by the DLL of hammer mill was researched,the development of user interface was completed.Characterized by the dimension-driven,the parameter-driven re-modeling of hammer mill was achieved,the whole model was changed automatically,and the procedural codes combined with specific examples were designed.As a result,a parametric modeling plug-in about the self-designed hammer mill was generated.The results show that this intelligent design system can modify the parameters of the fitted parts under hammer mill quickly and automatically,and output intuitive three-dimensional solid model of the assembly,which realizes intellectualization and automation of hammer mill design,speeds up the process of product design and enhances the efficiency

Online Soft-measurement for Thermal Efficiency of Circulating Fluidized Bed Boilers

提出循环流化床(Cfb)锅炉热效率的在线软测量方法,该方法将效率分为煤燃烧效率和传热效率两部分,根据Cfb锅炉动态机理模型在线计算锅炉未燃烧碳量,在此基础上计算煤燃烧效率,再通过反平衡法和正平衡法计算锅炉传热效率,最后得到锅炉热效率.应用该方法对某440T/H循环流化床锅炉热效率进行了计算,分析了影响该锅炉热效率的主要因素.结果表明:影响锅炉热效率的最主要因素是烟气中的飞灰含量;该软测量方法适用于Cfb锅炉,能有效指导锅炉调控,从而提高锅炉热效率.An online soft-measuring method for thermal efficiency of the circulating fluidized bed boiler(CFB)is being proposed.The method divides the efficiency into two parts,coal combustion efficiency and heat transfer efficiency.On-line calculation for the unburned carbon content of the boiler is carried out according to the dynamic mechanism model of the CFB boiler,after that combustion efficiency of the coal is calculated.Then heat transfer efficiency is calculated by indirect heat balance and direct heat balance,finally the boiler thermal efficiency is obtained.The thermal efficiency of certain 440 t/h CFB was calculated by the method,and the main factors which influence the thermal efficiency of the boiler were analyzed.Results show that the main factor which influences the thermal efficiency of the boiler is the content of fly ash in the flue gas.The soft-measuring method is suitable for CFB boiler,which can effectively direct the control of the boiler,thus improving the thermal efficiency of the boiler.福建省科技基金资助项目(2007J0165

Effects of Soil Salinity on the Quality of Carrot

采用土壤盆栽试验,设置6个盐度水平(0、500、1000、1500、2000、2500 Mg/kg),研究胡萝卜种植过程中盐度对其叶绿素、维生素C、可溶性糖、可溶性蛋白质及硝酸盐含量的影响。结果表明,种植胡萝卜的土壤盐度控制在500 Mg/kg,可以提高胡萝卜的品质,减少盐分对土壤的损伤。具体表现为,500 Mg/kg盐度处理对胡萝卜叶绿素含量无显著影响,而高盐度使胡萝卜叶绿素总含量降低;500~1000 Mg/kg盐度可增加胡萝卜维生素C和可溶性蛋白质含量;可溶性总糖含量以500 Mg/kg处理的降低幅度最小,500~2000 Mg/kg处理使胡萝卜硝酸盐含量呈降低趋势,但高盐度(2500 Mg/kg)则会增加其硝酸盐含量。A soil pot experiment with different levels of saltnity(0,500,1500,2000,2500 mg/kg) were carried out to study the effects of salinity on the quality of carrot.The results showed that controlling certain salinity 500 mg/kg,could improve the quality of carrot.Because the salinity of 500 mg/kg had no effect on the chlorophyll content of carrot,and high salinity caused total chlorophyll content decreased,low salinity was conducive to the promotion of carrot synthesis of vitamin C and soluble protein,soluble sugar content of carrot was decreased by the increasing of salinity levels,Nitrate content in carrots increased at the high salinity(2500 mg/kg) treatment.厦门市科学技术局项目(3502Z20071098

Online Soft-sensing and Optimization of CFBB Thermal Efficiency

循环流化床锅炉（CirculatingFluidizedBedBoiler，简称CFBB或CFB锅炉）是目前我国主要的清洁燃煤设备，具有良好的市场前景和发展前景。但在现有工业中，中小CFB锅炉普遍存在着锅炉效率偏低而煤耗偏高的现象。与大型CFB锅炉相比，中小型CFB锅炉仍存在较大的优化空间，为了获得更好的节能效果，有必要针对这部分CFB锅炉燃烧效率的优化控制方法进行研究。 目前中小型CFB锅炉存在着热效率偏低，操作人员依照经验调节锅炉参数等问题。文章以河南开封440t/h工业锅炉燃烧系统为研究对象，在本实验室已实现CFB锅炉燃烧过程在线闭环控制和锅炉效率、煤质在线观测的研究基础上，对CFB锅...Nowadays, Circulation Fluidized Bed Boiler (CFB Boiler or CFBB) is becoming the main coal combustion technique all over the world for its attractive advantages of high combustion efficiency and low gas emission. However, industrial CFB Boilers, especially medium and small types have low heat efficiency and they need more coal for burning. Compared to the big types, the medium and small boilers hav...学位：工学硕士院系专业：化学化工学院化学工程与生物工程系_化学工程学号：2062007115088