Preparation,identification and application of monoclonal antibody against urease subuint B of Helicobacter pylori

目的制备抗幽门螺杆菌(HP)的单克隆抗体(MAb),并对该HP MAb进行分析鉴定,建立一种检测患者由于HP感染而在血清中产生HP抗体的竞争ElISA检测方法。方法用灭活的HP免疫bAlb/C小鼠,通过杂交瘤技术制备HP MAb。我们采用HP混合蛋白包括毒素相关蛋白A(CAgA)、空泡毒素A(VACA)和尿素酶以及灭活的HP菌体筛选阳性杂交瘤细胞株,用ElISA和WESTErn blOT等技术对所获得的HP MAb进行鉴定。利用辣根过氧化物酶(HrP)标记所筛选的HP MAb来建立一个可检测患者血清中HP抗体的竞争ElISA。结果通过大规模的杂交瘤筛选,我们选择了1株将其命名为C3 HP MAb,其抗体亚型为Igg2A,腹水效价可达1x107。WESTErn blOT法、ElISA和质谱检测结果显示,该C3 HP MAb能特异地识别HP的尿素酶b亚单位。用这个C3 HP MAb,我们建立了一种可检测患者血清中HP抗体的竞争ElISA。结论成功获得一种可以特异识别HP尿素酶b亚单位的MAb,建立了一种可检测患者血清中HP抗体的竞争ElISA。Objective To prepare and characterize the monoclonal antibody(mAb) against Helicobacter pylori(Hp)and establish a competitive ELISA used for detection of Hp antibodies in the sera of Hp-infected patients.Methods BALB /c mice were immunized with inactivated Hp to generate Hp mAb using the hybridoma technology.Hp mixed proteins including cytotoxin-associated gene A(CagA),vacuolating cytotoxin A(VacA) and urease,as well as inactivated Hp were applied to screen positive hybridoma.Selected Hp mAb was analyzed and characterized with ELISA and Western blotting,and then labeled with horseradish peroxidase( HRP) for establishing a competitive ELISA to detect Hp antibodies in the sera of Hp-infected patients.Results One Hp mAb named as C3 was selected after screening large amount of hybridoma,and the C3 Hp mAb was special for IgG2 a subtype with the affinity titer of 1 × 107.Western blotting,ELISA and mass spectrum analysis indicated that the C3 Hp mAb could recognize Hp urease subunit B specifically.Using the C3 Hp mAb,we developed a competitive ELISA which could be used to detect Hp antibodies in the sera of Hp-infected patients.Conclusion We successfully obtained one mAb that could specifically recognize Hp urease subunit B and developed a competitive ELISA using the Hp mAb.福建省科技重点项目(2011Y0050); 厦门市科技计划项目(3502Z20123009); 国家基础科学人才培养基金(J1310027

Establishment of air pollutant emission inventory in the West Coast of Taiwan Strait

以2009年为基准年,结合污染源普查数据、统计年鉴及工业活动、居民生活等多个方面对海峡西岸经济区包括SO2、nOX、PM2.5、VOCS和nH3在内的大气污染物的排放量进行了估算,建立了海西区大气污染物排放清单.结果发现,上述5类污染物基准年的排放量分别为40.67x104、55.84x104、50.57x104、152.26x104和26.18x104T.其中,SO2、nOX及PM2.5的排放主要来自电厂,占排放总量的比例分别为25.58%、34.89%和38.75%;VOCS和nH3的主要排放源分别来自植被排放和养殖业,其贡献量分别为49.12%和47.07%.采用gIS对排放清单进行网格化处理,得出SO2、nOX及PM2.5的高排放强度区域与固定源的空间分布较为一致.此外,结合国家和地方“十二五“发展规划,采用情景分析方法估算了2015年海西区大气污染物的排放清单.与基准年相比,SO2、nOX和nH3的排放量呈下降趋势,PM2.5和VOCS的排放量呈大幅度增加.基准年排放清单的不确定性分析显示,VOCS排放估算的不确定度最大,为225%.The emission inventories of SO2,NOx,PM2.5,VOCsand NH3 in the Western Taiwan Straits Economic Zone in the base year of 2009 were established based on the pollution source census,statistical yearbooks,industrial activities and residential activities.The total emissions of SO2,NOx,PM2.5,VOCs and NH3 were estimated to be 40.67×104,55.84×104,50.57×104,152.26×104and 26.18×104t,respectively.The power plants were the most important source and contributed to 25.58%,34.89% and 38.75% of the total emissions of SO2,NOxand PM2.5,respectively.However,the major source of VOCs and NH3 was vegetation and livestock breeding,which contributed to 49.12% and 47.07% of the total,respectively.The relative high emission areas of SO2,NOxand PM2.5were consistent with the locations of stationary sources based on GIS gridding techniques.In addition,the emission inventories in the year of 2015 were calculated using scenario analysis method based on the national and regional 12thFive-Year Plans.The emissions of SO2,NOx,and NH3 showed a slight decrease while the emissions of PM2.5and VOCs increase significantly(around 24.18% and 74.42%,respectively) in 2015,as compared to those in 2009.The estimate of VOCs had the highest uncertainty of around 225%.环境保护公益性行业科研专项(No.201009004); 厦门大学山海基金(No.2013SH011)~

利用导电涂膜直接电解海水法防护生物腐蚀 1.模拟和测量技术

基于电化 学微探针 和微弱 信号检测 技术， 采用 四电 极体 系、双恒 电 位仪、锁相 相 关测定系统 以及三维 微调装 置建立模 拟和测量 系统，将 首次实 现对利 用导 电涂 膜直 接电 解海 水法 防护生物腐 蚀中微 米尺度内 痕量 Ｃｌ Ｏ － 浓度 的测量．本 文报道 了模拟和 测量实验 的阶段 性进展

Analysis of the Festo CLR Cylinder Performance

本文对Clr气缸工作性能的实验研究,目的是为了满足数控机床气动随行夹具中气缸的旋转夹紧要求。利用PlC控制器、气阀、气缸、传感器等元器件搭建实验平台。对主要实验步骤进行详细分析,通过实验论证fESTO Clr直线摆动夹紧气缸完全满足气动随行夹具工作要求。In this paper,we introduced an experiment to study the performance of pneumatic cylinder.In order to meet the requirements of the line/rotation clamping cylinder in CNC machine tools pneumatic clamp accompanied system.Using PLC controller,valves,cylinders,sensors and other components to build an experimental platform,the main experimental steps have been analyzed in details.Through the different kinds of experimental data demonstrating the Festo CLR line/rotation clamping cylinder is fit for the requirement of practical work

The application and research of the aircraft flexible fixture

主要研究气动、液压与电传技术在航空制造业中装配工艺中的应用。对研制出数控机床气动柔性夹紧系统,及其在飞机上零件加工上的应用进行了详细论述,包括柔性系统的机构组成、工作原理及PlC控制系统。该技术的突破为我国飞机数字化加工装配提供了参考价值,并将促进航空制造业的跨越式发展。The application of the pneumatic,hydraulic and power transmission for assembly process and equipment in aviation manufacturing industry is study.A novel type of CNC Aircraft Flexible Fixture machine system has been made out,and applied in manufacturing the aircraft parts.The system is including in architecture of the composition,working theory,PLC control system.It has a great signification of the manufacturing technology for aircraft digital processing,manufacturing and assembling,and promoting the developing of aircraft manufacturing

类泛素蛋白及其中文命名

泛素家族包括泛素及类泛素蛋白,约20种成员蛋白.近年来,泛素家族领域取得了迅猛发展,并已与生物学及医学研究的各个领域相互交叉.泛素家族介导的蛋白质降解和细胞自噬机制的发现分别于2004和2016年获得诺贝尔奖.但是,类泛素蛋白并没有统一规范的中文译名. 2018年4月9日在苏州召开的《泛素家族介导的蛋白质降解和细胞自噬》专著的编委会上,部分作者讨论了类泛素蛋白的中文命名问题,并在随后的\"泛素家族、自噬与疾病\"(Ubiquitinfamily,autophagy anddiseases)苏州会议上提出了类泛素蛋白中文翻译草案,此草案在参加该会议的国内学者及海外华人学者间取得了高度共识.冷泉港亚洲\"泛素家族、自噬与疾病\"苏州会议是由美国冷泉港实验室主办、两年一度、面向全球的英文会议.该会议在海内外华人学者中具有广泛影响,因此,参会华人学者的意见具有一定的代表性.本文介绍了10个类别的类泛素蛋白的中文命名,系统总结了它们的结构特点,并比较了参与各种类泛素化修饰的酶和它们的生物学功能.文章由45名从事该领域研究的专家合作撰写,其中包括中国工程院院士1名,相关学者4名,长江学者3名,国家杰出青年科学基金获得者18名和美国知名高校华人教授4名.他们绝大多数是参加编写即将由科学出版社出版的专著《泛素家族介导的蛋白质降解和细胞自噬》的专家

双膜传质模型在应用中的某些问题及修正方法

在采用化学吸收法测定气-液系统的传质比表面积和传质系数时,通常都沿用依据双膜理论导出的计算公式。但大量实验结果表明,这种处理方法存在着一定的问题,所得出的一些矛盾的结果无法解释。本文提出了关于相间传质双膜理论的一个修正模型以及相应的计算公式,所得出的结果与实验值符合得很好

利用抑制差减杂交技术分离三角褐指藻在缺氮条件下上调表达的基因

为了阐明硅藻利用氮源的分子机制,以三角褐指藻为材料,利用抑制差减杂交技术,分离鉴定了16个在缺氮诱导条件下上调表达的基因片段。其中,与已知功能基因具有较高相似性的有7种,都是跟氮源的吸收利用相关的。Northern blotting验证其中5个基因,包括硝酸盐转运蛋白基因、铁氧化还原蛋白亚硝酸还原酶基因、铵盐转运蛋白基因、结合ATP盒的转运蛋白基因和嘌呤透过酶基因,在缺氮诱导条件下转录水平有明显上调

基于双重双面法的弧齿锥齿轮双侧齿面同步修形设计

针对弧齿锥齿轮的双重双面法加工方法，以小轮齿槽中点作为双侧齿面修形的参考点，通过预置双侧齿面的啮合参数，构建符合啮合性能要求的目标齿面，建立基于加工参数优化的目标齿面误差数学模型和约束函数，采用非线性约束优化函数反求大轮的加工参数。根据齿面几何接触分析和有限元接触分析，对优化后的加工参数进行验证，结果表明，齿面修形后的啮合性能满足预期需求