The Application in GAP Production and Quality Control of glycyrrhiza uralensis Fisch.by TLC Fingerprint Spectrum

目的　研究甘草药材的薄层色谱指纹图谱 ,为科学评价及有效控制其质量提供可靠方法。方法　样品经预处理后点于硅胶 GF2 54预制板上 ,以乙酸乙酯 -乙酸 -甲酸 -水 ( 15∶ 1∶ 1∶ 2 )为展开剂 ,于 2 5 4 nm处进行吸收扫描。结果　甘草药材样品得到的薄层色谱指纹图谱有 6个共有峰 ,依此建立的柱状条形码图能快速地对药材品质作出评判。结论　采用 TL C指纹图谱可有效控制 GAP基地甘草的质量。Objective To set up a sensitive and specific TLC method for controlling the quality of glycyrrhiza uralensis Fisch..Method The sample solution was applied to the commercial pre-coated 20×10 cm silica gel F 254 plate (Merck) modified by 1% sodium hydroxide, ethyl acetate-formic acid-ethanoic acid-water(15∶1∶1∶2) as the solvent system, and quantitative analysis was carried out using UV absorptive scanning at 254nm. Result The TLC-FP of glycyrrhiza uralensis Fisch.showed 6 characteristic common peaks. The pole graph based on the height of common peaks can be conveniently used to evaluate the quality of different samples. Conclusion Quality of GAP glycyrrhiza uralensis Fisch. can be controlled effectively by TLC fingerprint.国家自然科学基金 2 0 0 3重点项目 ( 2 0 2 3 5 0 2 0

Determination of amino acids in Alisma orientale by pre-column derivatization of RP-HPLC

目的　分析不同产地泽泻中氨基酸含量及其特征性。方法　采用邻苯二甲醛 (OPA)、氯甲酸芴甲酯(FMOC)联合柱前衍生RP HPLC测定泽泻中的 17种氨基酸。结果　氨基酸质量浓度与峰面积呈良好的线性关系 ,r均在 0 99以上。不同产地泽泻中氨基酸含量有一定差别。结论　该方法适宜泽泻药材中氨基酸含量的测定。Object To determine the contents of amino acids a nd study the characters of Alisma orientale (Sam. Juzep.) from d ifferent habitats. Methods The samples of A. orientale from thr ee different areas were derived with o-phthaldialdehyde (OPA) a nd 9-fluorenylmethyl chloroformate (FMOC) in pre-column, and separated by RP- HPLC. Seventeen kinds of amino aci ds were determined. Results The concentration of amino acids and peak areas achieve d a nice linear relations (r is higher than 0.99). There were differences of the amino acid in A. orientale from diffe rent habitats. Conclusion This method is suitable for the determination of am ino acid in A. orientale.国家自然科学基金 2 0 0 3重点项目 ( 2 0 2 3 5 0 2 0 ) ;; 福建省泽泻GAP研究项目 ( 2 0 0 2Y0 2 4

HPLC指纹图谱应用于炙甘草的炮制研究

目的:应用HPLC指纹图谱规范炙甘草的炮制。方法:采用反相高效液相色谱法,DENALI C18色谱柱(VYDAC238DE5415,120A,5μm,4.6mm×150mm),以3%(v/v)HAc溶液和甲醇为流动相,梯度洗脱,柱温40℃,检测波长252nm。结果:炙甘草的HPLC指纹图谱有20个共有峰。传统炒制法炮制的炙甘草样品有一部分峰的重现性RSD值>3%;烘制法炮制的样品重现性有2个共有峰相对峰面积比的RSD>3%;微波法炮制的样品重现性符合指纹图谱的要求,20个共有峰相对峰面积比的RSD值均小于3%。结论:烘制和微波加热的炮制方法,可控性强,样品的指纹图谱重现性高,可以作为替代传统炒制工艺的新工艺。国家自然科学基金2003重点项目(20235020

微乳薄层色谱法鉴别甘草的研究

国家自然科学基金重点项目(20235020

Determination of Glycyrrhizin and Liquiritin in Licorice(Glycyrrhiza) by HPLC Method

目的测定甘草中甘草酸和甘草苷的含量。方法采用反相高效液相色谱法,DENALIC18色谱柱(VYDAC238DE5415,120A,5μm,4.6 mm×150 mm),梯度洗脱,流动相A∶H2O,流动相B∶1.5%HAc-M eOH,流速1.0 m.lm in-1,检测波长在32.2 m in从330 nm换为252 nm,从35 m in换为330 nm,柱温40℃。结果甘草苷的浓度在10~50μg.m l-1之间与峰面积线性关系良好,平均回收率为99.97%,方法精密度RSD=0.12%(n=5),甘草酸的浓度在50~90μg.m-l1之间与峰面积线性关系良好,平均回收率为100.25%,方法精密度RSD=0.13%(n=5)。结论该方法可用于甘草中甘草酸和甘草苷的同时含量测定。ObjectiveTo determine the content of glycyrrhizin and liquiritin in licorice(Glycyrrhiza).MethodsRP-HPLC method was estabilished.The analytical column was DENALI C18(VYDAC 238DE5415,120A,5μm,4.6 mm×150 mm),and gradient elution with A:H_2O,B:1.5% HAc-MeOH as mobile phase.The flow-rate was 1.0 ml·min~(-1).The detection wavelength was from 330nm to 252 nm at the 32.2 min,and then returned to 252 nm at the 35 min.The column temperature was 40℃.ResultsThe content of liquiritin was good linear related to its peak area in range of 10～50 μg·ml~(-1),and the average recovery of the method was 99.97%,the RSD of precision test was 0.12%(n=5).The content of glycyrrhizin was good linear related to its peak area in range of 50～90 μg·ml~(-1),and the average recovery of the method was 100.25%,the RSD of precision test was(0.13)%(n=5).ConclusionThe method can be used for determing the content of glycyrrhizin and liquiritin in licorice at the same time.国家自然科学基金2003重点项目(No.20235020

Discussion on the Feasibility Applied Fingerprint Chart in Quality Control of Chinese Herbs Pieces

　为促进中药饮片质量的研究,通过对文献资料的归纳、分析,对应用各种方法建立中药标准炮制品的指纹图谱库,并以指纹图谱相似度来评价中药饮片质量的可行性进行综合评述。中药指纹图谱在实现对中药饮片进行科学、客观、公正、有效的质量控制方面将起到重要的作用。In order to promoting the quality research of Chinese herbs pieces, the authors summed up and analyzed many literature information, and synthetically commented the feasibility applied the similarity of fingerprint chart in quality control of Chinese herbs pieces. It would show a important effect in impersonally and effectively controlling the quality of Chinese herbs pieces

中国大陆民办高校的分类与评估

大陆民办高等教育经历了二十多年的快速发展 ,与公办高校相比 ,目前民办高校不仅表现在类型上的多样化 ,其发展特性也有所不同。按大陆相关法规规定 ,公办和民办高校的认证标准是一样的 ,但民办高校各自的质量保障及评估控制体系仍然有着区别。本文对大陆民办高校类型与评估进行了调研及分类分析 ,并指出了民办高校发展亟待解决的尖锐问

Effect of Ti Doping on the Structure and Performance of LiNi_(0.75)Co_(0.25)O_(2)

应用溶胶-凝胶法合成LiNi(0.75-x)Co0.25TixO2(x=0,0.1,0.25)系列正极材料,其结构、形貌、粒度、电化学性能由TG、XRD、SEM和电池充放电测试表征研究表明,材料的电化学性能与钛掺杂量密切相关.在钴含量不变的情况下,随着Ti含量(x)的增加,材料由六方层状结构逐渐向立方结构转变,x=0.25时,出现了立方相与六方相共存.根据实验和理论计算结果简要讨论了钛掺杂对正极材料LiNi0.75Co0.25O2结构和电化学性能的影响.In this study,a series of Co-doped compounds with the formula of LiNi_((0.75-x))Co_(0.25)Ti_(x)O_(2)(x=0,(0.1),0.25)was synthesized by using sol-gel method.Their structure,particle size,electrochemical properties were studied by TG,XRD,SEM and electrochemical tests.It was shown that the structure of materials changed from a hexagonal layered structure to a cubic structure as the x value increased.In particular,a mixture of hexagonal and cubic structure was presented at x =0.25.The electrochemical performance of the materials depended strongly on the amount of Ti doping.Possible effects on the structure and performance of LiNi_((0.75-x))Co_(0.25)Ti_(x)O_(2) due to Ti doping are briefly discussed based upon the experiment and computational results.作者联系地址：厦门大学化学系材料科学与工程系固体表面物理化学国家重点实验室,厦门大学化学系材料科学与工程系固体表面物理化学国家重点实验室,厦门大学化学系材料科学与工程系固体表面物理化学国家重点实验室,厦门大学化学系材料科学与工程系固体表面物理化学国家重点实验室 福建厦门361005,福建厦门361005,福建厦门361005,福建厦门361005Author's Address: *,WANG Shu-fenDepartment of Chemistry,Department of Materials Science & Engineering,State Key Laboratory for Physical Chemistry of Solid Surfaces,Xiamen University,Xiamen 361005,Fujian,Chin

Impactfactors and optimization method on the separation of active components in licorice by micellar thin layer chromatography

第一作者Tel: 15012701322; E－mail: [email protected][中文文摘]目的:为甘草的胶束薄层色谱指纹图谱寻找最优胶束流动相。方法:首先采用单因子法,寻找影响甘草胶束薄层色谱的影响因素,在此基础上,采用控制加权可变步长单纯形优化法进行甘草胶束薄层色谱指纹图谱的流动相优化。结果:对甘草的胶束薄层色谱分离条件(表面活性剂的种类和含量、醇和酸改性剂的影响等)进行了实验,表明纯胶束薄层色谱的柱效较低,加入醇和酸类改性剂后柱效有明显提高。通过对改性胶束的进一步优化(控制加权可变步长单纯形优化法),得到优化的甘草改性胶束展开剂组成为:0.23 mol.L-1的SDS+16%(v/v)正丁醇+11%(v/v)甲酸。本研究对胶束薄层色谱的一些分离机理亦进行了探讨。结论:胶束薄层色谱的表面活性剂和各添加剂间存在交互作用,需采用合适的优化方法,才能达到分离中药材复杂活性成分群的目的。[英文文摘]Objective: To seek the best mobile phase of micellar thin-layer chromatography(MTLC)of licorice fingerprint.Method: Single impact factor of mobile phase in licorice MTLC fingerprint was tested individually and controlled weighted centroid simplex method was used finally to optimize the mobile phase of licorice MTLC fingerprint.Results: The selectivity of micellar TLC was studied for the separation of active components in licorice under various operation variables including concentrations of surfactant and co-surfactant,surfactant type,addition of acid modifier,et al.The results show that the low chromatography efficiency of micellar TLC could be improved by the addition of alcohol and acid modifier.Using controlled weighted centroid simplex ethod,optimization micellar TLC developing system of licorice was achieved which consists of 0. 23 mol·L－1 SDS,16%( v /v) n －butanol and 11% ( v /v) methyl acid.In addition,the separation mechanism of micellar TLC was also discussed.Conclusion:he appropriate optimization method should be tried to get the reliable mobile phase for the alternate impact of several parameters in MTLC国家自然科学基金资助项目(No.20235020

Impactfactors and optimization method on the separation of active components in licorice by micellar thin layer chromatography

第一作者Tel: 15012701322; E－mail: [email protected][中文文摘]目的:为甘草的胶束薄层色谱指纹图谱寻找最优胶束流动相。方法:首先采用单因子法,寻找影响甘草胶束薄层色谱的影响因素,在此基础上,采用控制加权可变步长单纯形优化法进行甘草胶束薄层色谱指纹图谱的流动相优化。结果:对甘草的胶束薄层色谱分离条件(表面活性剂的种类和含量、醇和酸改性剂的影响等)进行了实验,表明纯胶束薄层色谱的柱效较低,加入醇和酸类改性剂后柱效有明显提高。通过对改性胶束的进一步优化(控制加权可变步长单纯形优化法),得到优化的甘草改性胶束展开剂组成为:0.23 mol.L-1的SDS+16%(v/v)正丁醇+11%(v/v)甲酸。本研究对胶束薄层色谱的一些分离机理亦进行了探讨。结论:胶束薄层色谱的表面活性剂和各添加剂间存在交互作用,需采用合适的优化方法,才能达到分离中药材复杂活性成分群的目的。[英文文摘]Objective: To seek the best mobile phase of micellar thin-layer chromatography(MTLC)of licorice fingerprint.Method: Single impact factor of mobile phase in licorice MTLC fingerprint was tested individually and controlled weighted centroid simplex method was used finally to optimize the mobile phase of licorice MTLC fingerprint.Results: The selectivity of micellar TLC was studied for the separation of active components in licorice under various operation variables including concentrations of surfactant and co-surfactant,surfactant type,addition of acid modifier,et al.The results show that the low chromatography efficiency of micellar TLC could be improved by the addition of alcohol and acid modifier.Using controlled weighted centroid simplex ethod,optimization micellar TLC developing system of licorice was achieved which consists of 0. 23 mol·L－1 SDS,16%( v /v) n －butanol and 11% ( v /v) methyl acid.In addition,the separation mechanism of micellar TLC was also discussed.Conclusion:he appropriate optimization method should be tried to get the reliable mobile phase for the alternate impact of several parameters in MTLC国家自然科学基金资助项目(No.20235020