A Study of Chinese Teaching at Rotorua Boys' High School Confucius Classroom in New Zealand

本文以新西兰罗托鲁瓦男子高中孔子课堂的汉语教学情况为调查对象，主要运用调查问卷、课堂观察、访谈等研究手段进行调查和研究。 文章共分为六个章节：第一章为绪论；第二章介绍该校的总体情况，并明确学校突出的特点。之后在介绍新西兰中学汉语教学历史的基础上，概括出该校汉语教学历史的三个阶段：初创期、发展期和稳定期。 第三章从教学的四个要素：教学内容（课程和教材）、教师、学生和教学方法入手，阐明和分析该校的汉语教学现状。第四章则调查分析学生的学习行为。这两章是论文的主要章节。此两章得出的主要结论有：1、通过对问卷的统计，得出汉语学科在该校低年级学生中更受欢迎，尤其最受十年级学生的欢迎。2、通过运用“My...This paper takes the Chinese teaching situation of Rotorua boys' high school Confucius classroom as the object. The main means of investigation are survey research, classroom observation, on-the-spot investigation, interviews and other research methods. The article has six chapters. The first chapter is the introduction. The second chapter introduced the general situation of Rotorua boys' high sc...学位：汉语国际教育硕士院系专业：海外教育学院_汉语国际教育硕士学号：2652014115126

正交试验优选当归水提取工艺条件的研究

当归为多年生伞形科草本植物,主产于甘肃、云南、四川等地,根部入药,功能主治补血活血,调经止痛,润肠通便,其主要有效成分是阿魏酸。阿魏酸的化学名称为4-羟基-3-甲氧基肉桂酸,有顺式和反式两种,顺式为黄色油状物。反式为白色至微黄色结晶物,一般系指反式,分子量194.19,熔点174℃,微溶于冷水,可溶于热水。阿魏酸具有很强的抗氧化活性和抗菌活性,除了医药,在食品、化妆品、液晶、饲料添加剂等行业都有很广泛的用途

成人获得性维生素K依赖性凝血因子缺乏症伴多部位出血1例

患者,男,62岁,因“反复牙龈出血,血尿3月余,腹痛,便血3 d。“为主诉入院。患者缘于入院3月余前无明显诱因始出现反复牙龈出血,偶伴血尿,无尿频、尿急、尿痛;也无发热、畏冷;曾在当地治疗,好转后出院,入院3 d前无明显诱因出现腹痛,脐周明显,伴排稀水便,初为黑色,后为鲜红色,2~3次/d,无恶心、呕吐,也无返酸、嗳气。查体:体温36.2℃、脉搏96次/MIn、呼吸20次/MIn、血

Hg、Cd及其共同作用对烟草叶绿素含量及抗氧化酶系统的影响

本文报道了Ｈｇ、Ｃｄ及二者共同作用明显不同程度地影响烟草叶绿素含量及抗氧化酶系统。受Ｈｇ、Ｃｄ胁迫后，随着土壤中Ｈｇ、Ｃｄ浓度的增加，叶绿素含量、叶绿素ａ／ｂ值、ＣＡＴ活性逐渐减小，ＳＯＤ活性先升后降，ＰＯＤ活性则逐渐增加。同时也表现出单一Ｈｇ、Ｃｄ对烟草叶绿素含量及抗氧化酶系统的影响明显大于Ｈｇ、Ｃｄ二者的共同作用

The Acetylation of Thymosin α1 is Independent on RimL in Escherichia coli

目的:考察大肠杆菌乙酰转移酶rIMl对胸腺素α1(Tα1)乙酰化修饰的影响。方法:构建含500bP同源臂的卡那抗性基因打靶片段,利用rEd同源重组系统,使大肠杆菌bl21(dE3)的rIMl基因插入失活,随后导入质粒PCP20去除抗性基因,构建突变菌株rIMl-bl21(dE3);将重组质粒PET-Tα1-l12分别转入出发菌株和突变菌株中进行表达,经固定金属离子亲和层析和反向高效液相层析后,将所得纯品进行质谱分析,精确测定相对分子质量。结果:PCr鉴定结果证明成功敲除rIMl基因;质谱结果表明,rIMl基因敲除菌中所表达的Tα1-l12融合蛋白与出发菌株一样,均有部分乙酰化修饰。结论:Tα1的乙酰化修饰并不依赖于rIMl。Objective:To investigate the effect of RimL,a N-terminal acetyltransferase of E.coli,on thymosin α1(Tα1) acetylation.Methods:A kanamycin cassette with two 500 bp long arms homologous to the regions around rimL as the replacement fragment was electroporated into cells,in which the Red recombinant functions was induced.Subsequently,the plasmid pCP20 was transformed to eliminate the kanamycin resistant gene.The plasmid pET-Tα1-L12 was transformed into the original and mutant strains respectively.The expressed fusion protein Tα1-L12 was purified by IMAC and RP-HPLC.The accurate molecular weight of the fusion protein was measured by Q-Tof-MS.Results:The gene rimL was inactivated by insertion of the replacement fragment successfully.According to the MS results,the fusion protein Tα1-L12 was still partly acetylated when it was expressed in the mutant strains as in the original strains.Conclusion:Tα1 acetylation was independent on RimL

Expression of Fusion Protein of Parathyroid Hormone and Transferrin N-terminal Half-molecule in Pichia pastoris

利用重叠PCR技术将PTH(parathyroidhormone,甲状旁腺激素)基因与TFN(transferrinN_terminalhalf_molecule,转铁蛋白N端半分子)基因在体外融合,融合基因克隆至真核表达载体pPIC9中,转化毕赤酵母GS115。转化子经甲醇诱导后,融合蛋白得到了表达并分泌到发酵上清液中。经SPSepharoseFF阳离子交换层析、PhenylSepharoseFastFlow疏水层析纯化获得了纯度大于95%的PTH_TFN样品。Westernblot分析及腺苷酸环化酶实验证明融合蛋白中的PTH具有与抗PTH抗体结合能力及刺激腺苷酸环化酶的活性,铁饱和实验证明融合蛋白中的TFN和单独的TFN具有相同铁结合能力。因而TFN可望作为PTH的天然运输载体。The fused gene (PTH_TFN) of parathyroid hormone (PTH) gene and transferring N_terminal half_molecule (TFN) gene was amplified by multiple PCR and inserted into pPIC9 vector. The recombinant plasmid pPIC9_PTH_TFN was transformed into Pichia pastoris GS115 by PEG. After methanol induction, the target protein was expressed in fermentation supernatant at high level.The fused protein PTH_TFN with purity being higher than 95% was finally obtained after purification through two_step chromatography : SP Sepharose Fast Flow and Phenyl Sepharose Fast Flow.Western blot analysis and adenylate cyclase assay proved that the fused protein exhibited the bioactivity to stimulate cAMP synthesis and the ability to bind Fe ~3+ in the Fe ~3+ saturation study as the recombinant TFN did indicating that TFN could be used as the transcellar carrier of PTH.国家高技术研究与发展项目基金资助(No.2004AA215172)。~

Green Emission from a Strain-Modulated InGaN Active Layer

Strain-induced quantum dots (QDs) like island formations are demonstrated to effectively suppress pits/dislocation generation in high indium content (26.8%) InGaN active layers. In addition to the strain redistribution in the QD-like islands, strain modulation on the InGaN active layers by using the GaN island capping is employed to form an increased surface potential barrier around the dislocation cores, which inhibits the carrier transport to the surrounding dislocations. Cathodoluminescence shows distinct double-peak emissions at 503 nm and 444 nm, corresponding to the In-rich QD-like emission and the normal quantum well emission, respectively. The QD-like emission becomes dominated in photoluminescence due to the carrier localization effect of In-rich InGaN QDs at relatively low "carrier injection current". Accordingly, green emission may be enhanced by the following origins: (1) reduction in pits/dislocations density, (2) carrier localization and strain reduction in QDs, (3) strain modulation by GaN island capping, (4) enhanced light extraction with faceted GaN islands on the surface.National Natural Science Foundation of China [60876008, 61076091]; Program for New Century Excellent Talents in Fujian Province Universit

Cd、Pb胁迫对烟草叶片中活性氧清除系统的影响

Ｃｄ、Ｐｂ胁迫对烟草叶片活性氧清除系统的影响表明：随着Ｃｄ、Ｐｂ处理浓度的增大，叶绿素含量、叶绿素ａ／ｂ值，ＣＡＴ（过氧化氢酶）活性逐渐减小；ＳＯＤ（超氧化物歧化酶）活性先升后转而下降；ＰＯＤ（过氧化物酶）活性则逐渐增加。对烟草活性氧清除系统的影响，Ｃｄ、Ｐｂ之间存在明显的协合作用。Ｃｄ、Ｐｂ胁迫明显地影响活性氧清除系统，导致烟草叶片活性氧代谢失调和加速叶片的老化

有色金属价格指数关联性的VAR分析

本文采用上海有色金属价格指数,对2009年1月1日至2010年7月2日之间的铝、铜、镍、铅、锡、锌金属价格波动进行研究。结果表明,上述六种主要有色金属价格之间的联动性,是有色金属市场结构本身具有的特性之一。上述六种主要有色金属市场间,的确存在着长期稳定的均衡关系,一个市场价格波动对其他市场将产生显著的影响,其间时滞是短暂的