Preparation and the Cytotoxicity of Conjugate Composed of Pingyangmycin and Monoclonal Antibody against Human Lung Cancer

用dEXTrAnT40为中介体的方法交联抗人肺腺癌单抗3d3和平阳霉素（A5），每克分子抗体可携带68克分子的药物，交联物中抗体活性仍能达到10-4水平，能较好地与靶细胞l-342结合。体外细胞毒实验，显示交联物和游离A5对靶细胞50%杀伤浓度分别为0.9μMOl/l和4.6μMOl/l，杀伤效果比游离A5强5倍多。单抗对靶细胞几乎没有杀伤作用；无关抗体交联物的作用也很弱。交联物和游离A5对非靶细胞MgC-803的杀伤作用无明显差别。结果提示MCAb3d3具有良好的导向作用，能携带结合的A5特异地杀伤肺腺癌l-342细胞，可作为临床抗肿瘤药物导向治疗的一种载体。The conjugate of pingyangmycin (A5) and monclonal antibody3D3(MCAb3D3) against human lung cancer was prepared with dextran T40 as the mediator.The molar ratio of A5 McAb3D3 in the conjugate was 68:1 the titer of the conjugate was remained at 10-4.In vitro,the cytotoxicity of the conjugate and Free A5 yieded IC50 of 0.9μmol/L and 4.6μmol/L,respectivel.The conjugate was Fivetimes stronger than Free A5 The cytotoxicity of the conjugate on non-target cells MGc-803 was much weaker and similar to that of.Free A5.There is no doubt that MCAb3D3 had targeting activity and could kill or damage tumor cells speciFically and could be taken as a kind of vector in studies of clinical targeting therapy

纳氏测氨法半定量诊断HP感染的方法及其应用

对纳氏测氨法尿素酶试验（ＮＵＴ法）进行半定量标准化后，进一步建立了纳氏测氨半定量诊断人胃粘膜中幽门螺杆幽（ＨＰ）感染程度的方法，使ＮＵＴ法可同时用于定性及半定量诊断ＨＰ感染及感染程度．对经过半定量检测的３５０例患者作分析，显示轻、中、重度感染比率分别为５４．６％、３４．０％及１１．４％，另对其中１８２例慢性浅表性胃炎进行病变程度与ＨＰ感染程度的相关性分析，表明慢性浅表性胃炎的病变程度与ＨＰ的感染程度呈明确的正相关性（ｐ＜０．０５），提示胃部疾病的恶性演变尚与感染程度有关

用荷瘤裸鼠模型研制抗人肝癌单克隆抗体

1985年Watanabe等报道:用去胸腺载瘤小鼠的脾细胞与小鼠骨髓瘤细胞SP 2/0融合获得抗结肠癌单克隆抗体之后,利用荷瘤裸鼠模型成功研制抗人肿瘤单抗的报道已相继出现:但所获单抗多为IgM,IgG类的单抗得率不高,本工作采用多次手术大部切除瘤块的方法延长荷瘤裸鼠的成活期,获得一株抗人肝癌IgG类单抗、其研制过程和结果报道如下

中国东南沿海港口外轮压舱水生物的调查

随机选取东南沿海港口的17艘外来船舶(含8条集装箱船和9条散货船)作为监测对象,进行压舱水浮游植物及动物的物种鉴定和丰度测定,并对监测数据进行统计学分析。检出分属于7个浮游植物门类和5个动物门类的309种外来压舱水生物(包括60种赤潮生物)。外轮压舱水生物的分布及生存状态与水样的水龄和盐度相关。船舶压舱水排放是大家熟知的外来水生生物入侵的主要媒介。本调查结果表明,中国东南沿海外来散货船的压舱水排放所具的潜在生物入侵风险比集装箱船更应受关注

Ultrastructural observation on the relation of H.pylori to the gastric epithelia in chronic gastrictis and in peptic ulcer

目的应用透射电子显微镜(TEM)研究幽门螺杆菌(HP)与慢性胃炎和消化性溃疡粘膜上皮的关系.方法75例患者胃窦部活检标本用戊二醛固定,在锇酸染色之前先用单宁酸处理,常规包埋,TEM观察(每份样品至少做4张半薄切片定位,再行超薄切片).受检患者接受6种其它检测HP方法试验.结果55例感染上HP的患者中有53例胃粘膜内有TEM检查到细菌.在单宁酸特别染色的超薄切片中清楚地显示HP通过糖萼与胃粘膜上皮相连.当HP呈克隆状生长繁殖时,邻近的粘液细胞以及颈粘液细胞变性,其特征是细胞朝腺腔的胞质部分溃烂,细胞空泡样变或大泡形成,甚至整个上皮脱落.综合许多的证据表明:位于腺腔HP吸引中性粒细胞迁移到上皮细胞的间隙中,或者进入腺腔内进行吞噬HP活动.结论用TEM诊断HP感染的敏感和特异性分别是96%和95%.单宁酸能较好地保存细胞的糖萼.呈克隆状生长、多带有质周池的HP是导致粘膜上皮,包括颈粘液细胞,发生一系列变性的原因.若HP感染持续存在,颈粘液细胞变性和再生交替进行.最后再生干细胞受损伤,结果导致慢性萎缩性胃炎发生.AIMS The relationship between Helicobacter pylori (Hp) and gastric epithelia in chronic gastritis and in petic ulcer was studied by transmission electron mi- croscopy (TEM).METHODS Seventy-Five gastric antral biopsy speci- mens From the patients examined by six other methods For Hp were Fixed in glutaraldehyde and treated with tanin acid beFore OsO_4 staining than routinely prooessed For TEM studies (at least 4 semi- thin sections oriented For ultrathin sections in each sample).RESULTS The bacilli were detected by TEM within gastric mucosa in 53 of 55 patients inFected with Hp.Ultrathin sections especially stained with tanin acid re- vealed clearly glycocalyx by which the bacillus was connected with the epithelium.As the bacilli grouped as colony and breed,the adjacent mucous cells degerated and characterized by erosion of the juxtalu- minal cytoplasm,vacuolation or blebs,even desqua- mation of cell.Evidence was accumulated to show that the baoilli were located in the lumen attracted neu- trophils which intended to migrate into intercellular space of epithelia or into the lumen to exert the eFFect of Hp phagocytosis.CONCLUSIONS The sensitivity and speciFicity of Hp diagnosis by TEM is respectively 96% and 95%.Tanin acid is suitable For the preservation of glycocalyx of cell.The colonized bacilli,usually with the wide periplasmic pools,contributed to the spectrum degen- eration of epithelia,including mucous neck cells.IF Hp inFection persists,the degeneration and regeneration of mucous neck cells alternatively carried on and ultimate- ly the generative stem cells were damaged,as the result,the chronic atrophy gastritis could occure.SupportedbyScienceFoundationoFXiamen.No.95801

Relationship of Helicobacter Pylori InFection with Peptic Ulcers and Dyspeptic Symptom

幽门螺杆菌与消化性溃疡、消化不良症的关系1林炳珍杨善民颜江华张长弓（厦门大学抗癌研究中心厦门361005）幽门螺杆菌（HElICObACTErPylOrI，HP）以很高的人群感染率及与慢性胃炎和消化性溃疡的高度相关性引起国内外学者高度重视［1，2］....Bacterial culture,histological examination,rapid urease test, and PCR are used to detect Helicobacter Pylori (HP) in biospy speciments From372 patients with upper gastrointestinal disorder.The patients of positive results tested by more than two methds are considered as HP inFection positive.The results indicate that the presence of HP organisms was detected in 76.6% of patients with only gastritis, 95.4% of those with peptic ulcers,and 100% of those with gastric carinoma.These imply that the presence of HP was strongly associated with chronic gastritis and peptic ulcers.HP colonized in gastric mucous by its spciFic Flagella and high urease activity.It might cause the degeneration and death of epithelial cells by releasing cytotoxin and producing ammonia to cells,and Finally resulted in inFlammation.It is important to prevent From peptic ulcers and gastric carcinoma by controling the prevalence and the spread of HP and eradicating HP inFection in the stomach

EFFect of Natural Antioxidant Isoverbascoside on the Multplication and Ultrastructure of Human Gastric Adenocarcinoma Cells

MgC80-3细胞经20μMOl/l抗氧化剂lSOVErbASCOSIdE处理后，细胞生长曲线与分裂指数显著下降，倍增时间延长，生长抑制率达57.8%.电镜观察结果表明：lSOVErbASCOSIdE处理的细胞，核质比例下降，核形规则，核仁体积缩小，数量减少，核内异染色质减少；线粒体形态结构典型；细胞表面微绒毛减少等显著变化.说明天然抗氧化剂ISOVErbASCOSIdE改变了MgC8O-3细胞的恶性表型特征，具有明显的诱导分化作用.Fter treatment with 20 μmol/L Isoverbascoside,the growth curve and mi-totic index of MGc 8O-3 cells decreased remarkably, cell doubling time prolonged,and cellulargrowth inhibitory rate amounted to 57.8%.The electron microscope revealed that the nuclear-cytoplasmic ratio decreased in treated cells,the shape of nuclei was regular,nucleouls lessened,neterochromatin reduced.In the cytoplasm, mitochondria were typical, the microvilli at cell’ssurFace reduced obviously.It is con Firmed that natural antoxidant Isoverbascoside could reverseMGc 8O-3 cell’s maligant phenotypic characteristics,and had certainly induced and diFFerentiatedrole For gastric adenocarcinoma cells.国家自然科学基金及福建省自然科学基

Analysis of Radioimmunological Image of131I-McAbJH107 in Nude Mice BearingHuman Liver Cancer BEL-7402

用氯胺T碘标技术标记抗人肝癌单克隆抗体JH107（MCAbJH107），观测其在载人肝癌bEl7402裸鼠模型上的分布显像情况，氯胺T碘标法标记率50%。每鼠腹腔注射200CI131Ⅰ-MCAbJH107，放射性物质第24H开始在肿瘤部位浓聚、逐步加强，96H达高峰，肿瘤组织较清楚显像并维持至148H；同时周围组织放射性本底逐步减弱、消失。对照组131Ⅰ-nIgg呈全身均匀分布。无明显放射性物质浓聚及清楚显像。在48H和96H，131Ⅰ-MCAbJH107在12种正常组织（脑除外）的T/nT均值分别为3.38和6.26，而131Ⅰ-nIgg的T/nT均值均低于1.0。he monoclonal antibody JH107,(McAbJH107)against human liver cancerwas labelled by the ch-T131 I-radiolabelling method.Its distribution andimage were detected in the nude mice bearing human liver cancer BEL-7402.Injecting 200 1 131 I-McAbJH107 to each mouse peritoneally the con-centrating of the radioactive substance at the site of the tumor began at24th and strengthened progressively, and reached its top at 96th.Theclearer image of tumor tissue was kept to 148th.At the same time,theradioactive background of the tissues around the tumor gradually disappea-red.In131I-NIgG of the control group well-distributed in the whole body,no signiFicant concentration and no clear image were observed.At 48 and96th, T/NT mean values of 131I-McAbJH107 in normal tissues of 12 kinds(except the brain ) were 3.38 and 6.26 respectively, and ones of 131I-NIgGwere lower than 1.0福建省计委、卫生厅”八五”科技攻关资