DHX32 promotes angiogenesis in colorectal cancer through augmenting β-catenin signaling to induce expression of VEGFA

肿瘤的生长和转移离不开肿瘤血管生成，因此抗血管生成是目前肿瘤治疗的重要途径之一，其中血管内皮生长因子VEGF及其信号通路是抗肿瘤治疗的重要靶点。DHX32是近年来新发现的一种RNA螺旋酶，对其在肿瘤发生发展中的作用研究刚刚起步。我们的前期研究表明DHX32在结肠癌中表达上调，并能够促进结肠癌细胞增殖、迁移和侵袭，抑制细胞凋亡；qPCR芯片的筛选结果显示敲低DHX32抑制了血管生成因子VEGFA的mRNA水平，预示着DHX32可能参与了肿瘤血管生成的调控。本研究发现在结肠癌细胞中DHX32通过与b-catenin结合并稳定其蛋白水平从而上调b-catenin下游靶基因VEGFA的转录；由此提高了...Colorectal cancer (CRC) is the third most common cancer worldwide and accounts for 14% of all new cancer diagnoses. Tumor angiogenesis that is required for cancer growth and metastasis by nourishing cancer cells and helping spread of metastatic cells to distant tissues has been considered as a potential target for CRC treatment. Understanding the molecular mechanism by which CRC cells promote angi...学位：理学博士院系专业：生命科学学院_生物化学与分子生物学学号：201317007

The Optimization Design of Performance Management of BLT Fuzhou Company

随着经济全球化进程的加速和信息技术的飞速发展，所有企业都面临一个充满变数的不确定环境。企业能否获得持续发展，能否在激烈的竞争中确立自己的竞争优势，主要取决于企业的战略管理能力，而企业的战略管理能力体现为企业战略性绩效目标的层层分解、分担和传递。因此，面对瞬息万变的企业内外部环境，如何搭建完善的战略绩效管理体系，并有效利用信息技术辅助企业战略管理过程，借助管理工具将战略目标落实到日常的绩效管理中，是一个优秀的战略人力资源管理系统要解决的问题。 人力资源管理的核心目标是支持企业战略发展。本文的研究目标是基于BLT福州公司目前正在推行的绩效管理优化，从理论高度剖析BLT福州公司如何借助战略绩效管理...As the process of economic globalization is accelerating and information technology is developing rapidly, all enterprises are faced with an uncertain environment full of changes. Whether an enterprise could achieve a sustainable development and establish its own competitive advantages mainly lies on its capability of strategic management, which is embodied in layer-by-layer distribution and trans...学位：工商管理硕士院系专业：管理学院工商管理教育中心（MBA中心）_工商管理硕士(MBA)学号：1792010115081

cAMP/PKA signaling plays a pivatol role in cispaltin-induced apoptosis by controlling Smurf1-mediated stabilization of Nur77

孤儿受体Nur77是早期应答基因表达的产物，属于类固醇/甲状腺受体超家族的成员，在细胞的增殖、分化发育和凋亡等生理过程中发挥了重要的调控作用。顺铂是一种应用广泛的化疗药物，能够通过调控细胞内的凋亡相关蛋白诱导肿瘤细胞凋亡以抑制肿瘤生长。近年来有文献报道，顺铂通过Nur77的介导能诱导直肠癌细胞凋亡从而抑制肠道肿瘤的发生和发展。我们实验室的另一部分工作表明Smurf1可以通过催化Nur77的非典型泛素化来抑制其蛋白酶体降解途径，在顺铂诱导的细胞凋亡中起着重要的作用。 cAMP作为胞内重要的第二信使之一，参与了细胞内多种关键生理功能的调节。目前的研究表明cAMP在不同的细胞中对于细胞的生长和死亡...The orphan nuclear receptor Nur77 is a product of an immediate-early gene and belongs to the steroid/thyroid/retinoid nuclear receptor superfamily. Nur77 regulates diverse cellular activities including cell proliferation, differentiation and apoptosis. Cisplatin is a chemotherapy drug widely used for the treatment of many malignancies, including testicular, bladder, cervical, ovarian, lung cancers...学位：理学博士院系专业：生命科学学院_微生物学学号：2162010015391

Cell-Based High-throughput Screen for Inhibitors of the E3 Ubiquitin Ligase Smurf1

可调控的蛋白质降解在维持细胞的正常生物学功能上有着极其重要的作用，非正常的蛋白质降解会导致包括癌症在内的许多疾病。E3泛素连接酶在特异性调控蛋白质分子的泛素化降解过程中起着至关重要的作用。针对特定的E3泛素连接酶的小分子调节剂可以特异性地调控某些特定底物蛋白的降解，从而调节特定的细胞生物学功能，这将为我们治疗如癌症、老年痴呆症等重大疾病提供有效的手段，从而成为非常有希望的药物先导化合物。因此，以特定的E3泛素连接酶作为靶点蛋白来进行药物筛选越来越受到研究者们的重视。 我们通过应用不同的荧光蛋白和泛素融合蛋白参照技术，提高了从细胞水平上进行药物筛选的信噪比，为建立E3泛素连接酶调节剂的高通量细...Regulated protein degradation is required for many important biological processes such as cell cycle, DNA repair, signal transduction, transcriptional regulation, endocytosis and stress responses[1-2]. Ubiquitin-dependent proteasome pathway is the key regulatory mechanism that controls the protein degradation. Accumulated evidence suggested that a subset of E3 ubiquitin ligases involve in tumorige...学位：理学硕士院系专业：生命科学学院生物学系_微生物学学号：2162007115193

卡铂注射液多疗程化疗致过敏性休克一例并文献复习

过敏性休克是外界某些抗原性物质进入已致敏的人体机体后,通过免疫机制在短时间内发生的一种强烈的多脏器受累症候群,在临床上不可预测的、突然发生的、危及患者生命的严重过敏反应[1]。据统计,首次接受卡铂化疗的患者,前5个疗程卡铂过敏发生率<1%,第6个疗程升高至6.5%,≥7个疗程者发生率高达27%,复发患者或累计

One New Ten-membered Lactone from Phomopsis sp. B27,an Endophytic Fungus of Annona squamosa L.

目的:研究番荔枝内生真菌拟茎点霉B27的化学成分。方法:通过色谱层析柱对提取物进行分离纯化,并通过波谱解析(一维、二维的核磁共振谱和质谱)确定化合物的结构。结果:分离纯化得到5个化合物,鉴定为十元大环内酯phomolide C(1),1-甲基-8-羟基-9,10-蒽醌(2),1,8-双羟基-9,10-蒽醌(3),cytosporone C(4)和Altiloxin A(5)。结论:化合物1是新化合物;化合物1-5首次从拟茎点霉属菌株中分离得到。AIM: To study the chemical constituents of Phomopsis sp. B27, an endophytic fungal strain of Annona squamosa L. METHODS: The extract was isolated and purified by column chromatography. The structures of chemical components were deter-mined by spectroscopic analyses including 1D-, 2D-NMR and MS data. RESULTS: Five compounds were isolated and determined as a ten-membered lactone, namely phomolide C (1), 1-methoxy-8-hydroxy-9,10-anthraquinone (2), 1,8-dihydroxy-9,10-anthra-quinone (1,8-DHA) (3), cytosporone C (4) and altiloxin A (5). CONCLUSION: Compound 1 is a new ten-membered lactone; and compounds 1-5 were isolated from the genus Phomopsis for the first time.the National Science Foundation for Distinguished Young Scholars to Y. Shen. (No.30325044).~

In situ dynamic tracking of heterogeneous nanocatalytic processes by shell-isolated nanoparticle-enhanced Raman spectroscopy

原位监测纳米催化反应过程对深入认识反应机理、设计高效催化剂具有重要意义。作为一种具有超高灵敏度的表面分析技术，表面增强拉曼光谱（SERS）是可提供反应过程中催化剂表面吸附物种的丰富信息。然而，仅有Au、Ag、Cu等少数金属在形成特定纳米结构时才能提供较强的拉曼增强，且它们会对催化剂的性质及反应过程产生严重干扰。这就极大地限制了SERS在实际多相催化体系中的应用。鉴于此，该论文发展了一种利用壳层隔绝纳米粒子增强拉曼光谱原位监测纳米催化过程的方法。通过将纳米催化剂组装于壳层隔绝纳米粒子表面，形成SHIENRS卫星结构，利用内核Au纳米粒子增强催化剂表面的拉曼信号，SiO2壳层隔绝Au对催化剂及反应的影响，从而直接获得了纳米催化剂表面物种的真实信息。利用这种SHINERS卫星策略，他们实现了CO氧化反应的原位监测，直接观测到了反应条件下催化剂表面吸附物种。并结合DFT计算，对反应机理进行了阐述。该研究表明SHINERS卫星策略可作为原位跟踪催化反应过程的有效方法，为纳米催化的原位研究提供了一种新的思路。 同时，我校也将在今年12月5-9日举办表面增强拉曼光谱国际会议(International Conference on SERS)，讨论SERS领域的最新进展。会议期间，SERS领域的先驱与权威Richard P. Van Duyne、Martin Moskovits、Andreas Otto以及相关学科的顶级学者Peter G. Bruce、Stefan A. Maier等将作大会报告（会议官方网站http://sers2017.xmu.edu.cn/）。【Abstract】Surface molecular information acquired in situ from a catalytic process can greatly promote the rational design of highly efficient catalysts by revealing structure-activity relationships and reaction mechanisms. Raman spectroscopy can provide this rich structural information, but normal Raman is not sensitive enough to detect trace active species adsorbed on the surface of catalysts. Here we develop a general method for in situ monitoring of heterogeneous catalytic processes through shell-isolated nanoparticle-enhanced Raman spectroscopy (SHINERS) satellite nanocomposites (Au-core silica-shell nanocatalyst-satellite structures), which are stable and have extremely high surface Raman sensitivity. By combining operando SHINERS with density functional theory calculations, we identify the working mechanisms for CO oxidation over PtFe and Pd nanocatalysts, which are typical low- and high-temperature catalysts, respectively. Active species, such as surface oxides, superoxide/peroxide species and Pd–C/Pt–C bonds are directly observed during the reactions. We demonstrate that in situ SHINERS can provide a deep understanding of the fundamental concepts of catalysis.This work was supported by the NSFC (21522508, 21427813, 21373167, 21521004, 21573178 and 21673187), Natural Science Foundation of Guangdong Province (2016A030308012), the Fundamental Research Funds for the Central Universities (20720150039 and 20720160046), ‘111’Project (B16029), and the Thousand Youth Talents Plan of China。 研究工作得到国家自然科学基金优秀青年基金、谱学分析创新研究群体和青年千人计划等资助

光子晶体光纤的特性与研究现状

光子晶体光纤(PCF)具有独特的光学特性和灵活的设计,在光通信等领域具有广阔的应用前景。文阐述了PCF的一些独特光学性质,总结了光子晶体光纤的研究成果。福建省自然科学基金资助项目(2006G0410);; 福建省自然科学基金资助项目(2006J0410

Two Epothilones from Sorangium cellulosum Strain So0157-2

目的:对粘细菌SOrAngIuM CEllulOSuM SO0157-2的化学成分进行研究。方法:通过色谱层析对提取物进行分离纯化,并通过波谱解析(一维、二维的核磁共振谱和质谱)确定了化合物的结构。结果:分别鉴定为SECO-EPOTHIlOnEA(1)和1-METHyl-SECO-EPOTHIlOnEA(2)。结论:化合物1和2都是首次从该菌株中分离得到。其中1是首次分离得到的新天然产物。AIM:To study the chemical constituents of Sorangium cellulosum So0157-2.METHODS:The chemical constituents were isolated and purified by column chromatography,and their structures were identified by spectroscopic analyses including 1D-,2D-NMR data and MS analyses.RESULTS:Two epothilones were purified and identified as seco-epothilone A(1) and 1-methyl-seco-epothilone A(2).CONCLUSION:1 and 2 were obtained from this strain for the first time,and 1 was a new natural product.supportedbyKeyProgramofInternationalCooperation;MinistryofScienceandTechnologyofChina(No.2007DFA30970);the863ProgramofChina(No.20060102Z1083)---

Comparison of life and psychological status of the elderly between families with children absent and families with children

目的　比较研究城市空巢老人与非空巢老人的生活和心理状况。方法　以《中脉老年生活质量指数调查问卷》为材料,对7个城市2 225位独居、夫妻同住和非空巢老人的调查数据进行分析。结果　大多数老年人的经济收入偏低,非空巢老人给予子女经济支持和体力付出的压力较大;大多数老年人的心情较好,但超过1 /4的独居老人心情较差;老年人的主观幸福感居中上水平,但独居老人的主观幸福感、与家人交流满意度较低;在养老依靠问题上空巢老人(尤其是独居老人)对于家人的期望较低;对于第三方赡养和老年人再婚的问题,夫妻同住老人比较理解和支持。 结论　夫妻同住老人的生活和心理状况均较好,独居老人的心理状况较差,非空巢老人的经济和体力付出的压力较大